Person: ELMACI, CENGİZ
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ELMACI
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CENGİZ
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Publication Genome-wide association studies of preweaning growth and in vivo carcass composition traits in esme sheep(Wiley, 2021-07-22) Yılmaz, Onur; Kızılaslan, Mehmet; Arzık, Yunus; Behrem, Sedat; Ata, Nezih; Karaca, Orhan; Elmacı, Cengiz; Cemal, İbrahim; ELMACI, CENGİZ; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Zootekni ve Hayvan Besleme Bölümü.; 0000-0003-4819-0221; V-7882-2019Sheep are considered as a major contributor of global food security. Moreover, sheep preweaning growth traits as well as in vivo carcass composition traits such as ultrasonic measurements of Longissimus dorsi muscle depth (UMD) and back-fat thickness (UFD) are crucially important indicators of meat yield and hot carcass composition. Despite their relative importance for productivity and profitability of a sheep production system, detected QTL for these traits are quite scarce. Therefore, we implemented GWAS for these traits using animal mixed model-based association approach provided by GenABEL in Esme sheep. Three genome-wide and 14 individual chromosome-wide associated SNPs were discovered. As a result, ESRP1, LOC105613082, ZNF641, DUSP5, TEAD1, SMOX, PTPRT, RALYL, POM121C, PHIP, LOC101106051, ZIM3, PEG3, TRPC7, FBXL4, LOC105610397, LOC105616489 and DNAAF2 were suggested as candidates. Some of the discovered genes and involved pathways were already annotated to contribute growth and development in various species including human, mice and cattle. All in all, the results of this study are expected to strongly contribute to shed a light on the underlying molecular mechanisms behind growth and carcass composition traits, with potential implications on studies aiming faster genetic improvement, targeted low-resolution SNP panel designs and genome-editing studies.Publication Growth hormone gene polymorphism in pekin ducks reared in Turkey(Namik Kemal Üniversitesi, 2022-12-01) Eriş, Candan; Elmacı, Cengiz; Eriş, Candan; ELMACI, CENGİZ; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Zootekni Bölümü; 0000-0003-4819-0221; HNL-1511-2023; DWS-5803-2022Advances in molecular genetics have led to the identification of genes or markers that affect many morphological and physiological characteristics of farm animals. The molecular basis of these traits is revealed by functional genomic methods and provides opportunities to develop genetic improvement programs in farm animals through marker assisted selection (MAS). Several candidate genes are being studied with the aim of increasing various yield and reproductive performances in animals through MAS. One of these candidate genes is the growth hormone gene. The main purpose of this study was to investigate polymorphisms in the 2nd, 3rd and 4th introns of the growth hormone gene in Pekin ducks. For this purpose, blood samples taken from a total of 117 adult Pekin ducks of both sexes (79 males and 38 females) raised in Bursa Uludag University Faculty of Agriculture Agricultural Application and Research Farm were used as material. Genetic polymorphism in the 2nd, 3rd and 4th introns of the growth hormone gene was investigated using the BsmF1 restriction enzyme in the PCR-RFLP method with three primer pairs. Samples were carried out in 2% agarose gel electrophoresis to separate the DNA fragments obtained after the digestion. As a result of the researches, polymorphism was determined only in the fragment amplified from the 2nd intron region. As a result of the digestion of the amplified fragment from the 2nd intron region with BsmF1 restriction enzyme, three genotypes, namely TT (765 bp), CT (765, 593 and 172 bp) and CC (593 and 172 bp) were determined in this region and the frequencies of the T and C alleles were calculated as 0.75 and 0.25, respectively. Since the difference between the values observed in the 2nd intron region of the growth hormone gene and the expected values in the studied population was not statistically significant (P>0.05), it was understood that the population in studied was in Hardy-Weinberg equilibrium. No genetic variation was observed in the 3rd and 4th introns. PCR products belonging to the 442 bp and 1378 bp regions of the 3rd and 4th introns of the growth hormone gene, respectively, were also treated with BsmF1 restriction enzyme, but the cleavage region of this enzyme was not found, so there was only one genotype was obtained in the 3rd and 4th introns of the studied population. Therefore, it was understood that these two regions are monomorphic in terms of BsmF1 locus. The genotypic variation shown in the 2nd intron region of the growth hormone gene in this and previous studies also reveals the need for further studies on the relationships between this region and various yields.Publication The estimation of live weight from body measurements in different meat-type lambs(Galenos Yayınevi, 2021-01-01) Esen, Vasfiye Kader; Elmacı, Cengiz; ELMACI, CENGİZ; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Zootekni Bölümü.; 0000-0003-4819-0221 ; DWS-5803-2022The present study aimed to analyze the live weight (LW) and some body measurements to estimate the LW of lambs in five different meat-type sheep breeds reared in the same flock under intensive conditions. A total of 202 head of lambs, including both genders of Kivircik (K), Bandirma (B), Karacabey Merino (KM), Hampshire Down x Merino crossbreed (HM), and Ramlic (R), were conducted in this study. Birth weights ranged between 3.94-5.07 kg in those breeds and were affected significantly by breed and birth types (P<0.001). The effect of sex was clearly seen with the weaning period; males lambs were 2.61 kg heavier than female lambs (P<0.001). The importance of breed differences on chest circumference (CC) increased as the lambs grew older. On the other hand, sex had a significant effect on all body measurements at the pre- and post-weaning period (P<0.01 and P<0.001); however, did not on body length (BL) at the pre-weaning period (P>0.05). In contrast, the importance of birth type on BL and CC decreased as the lambs grew older (P>0.05). The results of regressing LW and body measurements show that LW was very highly (P<0.001) correlated with body measurements (r=0.682-0.892). The highest correlations were observed between LW and CC (r=0.802-0.892) in B, KM, and R lambs. These results suggest that using body measurements as a correction factor has a great benefit in those breeds, such as withers height and CC are used to estimate LW in KM and R lambs, effectively.Publication Distributions of CYP19, ERα and PGR allele frequencies between fertile and subfertile Holstein-Friesian heifers(Kafkas Üniversitesi, 2015-11-01) Keskin, Abdulkadir; Öner, Yasemin; Yılmazbaş-Mecitoğlu, Gülnaz; Güner, Barış; Karakaya, Ebru; Elmacı, Cengiz; Gümen, Ahmet; KESKİN, ABDULKADİR; ÖNER, YASEMİN; MECİTOĞLU, GÜLNAZ; Güner, Barış; Karakaya, Ebru; ELMACI, CENGİZ; GÜMEN, AHMET; Uludağ Üniversitesi/Veteriner Fakültesi/Jinekoloji Anabilim Dalı.; Uludağ Üniversitesi/Ziraat Fakültesi/Hayvan Bilimleri Biyometri ve Genetik Bölümü.; 0000-0002-2904-8986; 0000-0003-2817-3221; 0000-0001-6414-6752; 0000-0003-4837-1858; 0000-0003-4819-0221; 0000-0002-3607-4365; V-7882-2019; AIF-3815-2022; ABB-3181-2020; AAH-3831-2021; AAH-1406-2021; AAG-4440-2019; AGY-3496-2022; ABG-2047-2020The aim of this study was to investigate the gene and genotype distributions of some mutations in the aromatase cytochrome P450 (CYP19), estrogen receptor alpha (ER alpha), and progesterone receptor (PGR) genes in fertile and subfertile Holstein-Friesian heifers using the PCR-RFLP method and comparing the distributions between groups. A total of 106 heifers were included the study, and the heifers that became pregnant after the first artificial insemination (n=51) were used as a fertile group. Heifers (n=55) with equal and more than 3 AIs were accepted as a subfertile heifers. Blood samples from all of the heifers were obtained for DNA isolation. While two alleles and three genotypes were found at the PGR and ER alpha loci, two alleles and two genotypes were detected at the CYP19 locus. The A allele and AA genotype, G allele and GG genotype, and C allele and CT genotype were found to be predominant in CYP19, ER alpha and PGR, respectively. According to the chi-square test (chi(2)), two of the groups investigated were in Hardy-Weinberg equilibrium for all gene loci. There were no differences detected in allele or genotype frequencies between the fertile and subfertile heifers.Publication Effects of beta vinasse supplementation on performance, meat quality and iliocaecal microflora in quail rations(Hellenic Veterinary Medical Soc, 2022-10-01) Çetin, I.; Cengiz, S. S.; Çetin, E.; Sarpasar, M.; YEŞİLBAĞ, DERYA; ELMACI, CENGİZ; Bursa Uludağ Üniversitesi/Veteriner Fakültesi; 0000-0003-0979-8402The aim of this study was to evaluate the effects of Beta (beta)-vinasse supplementation on the live weight (LW), live weight gain (LWG), feed intake (FI), feed conversion ratio (FCR), carcass yield, meat quality, and ilio-caecal bacteriological flora of quails. A total of 240 5-d-old Japanese (Coturnix coturnix Japonica) quails, including both males and females, were divided into 3 groups 80 quails and treated as follow: a control group (1) with 0 g beta-vinasse/ kg ration; (2) 15 g/kg beta-vinasse and (3) 30 g/kg beta-vinasse. The study lasted for 6 weeks. At the end of the experiment, supplementation with beta-vinasse did not have a significant effect on FI and FCR. Dietary supplementation with 30 g/ kg beta-vinasse significantly (P < 0.05) increased LW (21 d)and LWG (5 to 21 d). The dietary treatment of quails with different levels of beta- vinasse did not affect hot carcass weight, cold carcass weights, hot and cold carcass yields, and breast and thigh pH.The lightness (L*) and yellowness (b*) of breast values were significantly (P<0.05) increased by 30 g/kg supplementation. Different levels of beta-vinasse significantly increased Lactobacillus spp. in faeces. As a result, it was concluded that beta-vinasse (by-product obtained from molasses) can be used in quail diets as an alternative feed source that will meet the nutritional needs of the animal and have positive effects on the digestive system, especially on the intestinal health (an increase in Lactobacillus spp. counts).Publication Genotypic characterization of meat-type lambs expressing the callipyge gene in turkey: I. carcass characteristics and retail yield(Tübitak Bilimsel ve Teknolojik Araştırma Kurumu Türkiye, 2022-01-24) Esen, Vasfiye Kader; Esen, Selim; Karadağ, Orhan; Onenc, Alper; Elmacı, Cengiz; ELMACI, CENGİZ; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Zootekni Bölümü; 00000-0003-4819-0221; DWS-5803-2022This study is set out to evaluate the effects of callipyge (CLPG) gene, attributed to sheep affecting growth and meat quality, on carcass and sensory trait of most preferred meat-type lamb (Kivircik, Karacabey Merino, Ramlic, German Black-Head Mutton x Kivircik, Hampshire Down x Merino) in the western part of Turkey. Two datasets were used: (i) 177 lambs (66 males, 111 females) to determine genotype and allele frequencies; (ii) 48 genotypically identified male lambs for the assessment of carcass and meat sensory quality. It was found that homozygous (NN) and heterozygous (MN) conditions of the CLPG gene were observed in all sheep breeds, except Ramlic which exhibits only NN. No significant differences were observed in CLPG genotypes for slaughter weight, cold carcass weight, and cold dressing percentage (p > 0.05). On the other hand, the effect of genotype on neck percentage was significant (p < 0.01), and lambs expressing CLPG genotype (MN) had a higher neck percentage. Not significantly, but numerically the MN genotype had a higher percentage of the shoulder, rack, and leg than the NN genotype, while the NN genotype had a higher percentage of loin (p > 0.05). Similarly, the effect of genotype on meat quality assessment was not significant (p > 0.05); however, shear force, water holding capacity, and cooking loss of the NN genotype were higher than the MN genotype. Indeed, no interaction between genotype and time was observed on the color parameter of the Longissimus thoracis et lumborum muscle at various storage periods (p > 0.05). The findings suggest that all meat-type sheep breeds (except Ramlic) were polymorphic, suggesting that heterozygous individuals who received the CLPG mutation from the sire should be adopted in selection programs to improve carcass traits.Publication Genotypic characterization of meat-type lambs expressing the callipyge gene in Turkey: II. effect on body indexes(Elsevier, 2022-02-04) Kader-Esen, Vasfiye; Esen, Selim; Karadağ, Orhan; Elmacı, Cengiz; ELMACI, CENGİZ; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Zootekni Bölümü.; 0000-0003-4819-0221; V-7882-2019This study is set out to examine the relationship between the callipyge (CLPG) gene, attributed to sheep affecting growth and meat quality, and body indexes of meat-type sheep breeds to assess its feasibility in using it genomic selection programs. For this purpose, blood samples were collected from 177 lambs (66 male, 111 female) of five different sheep breeds (Kivircik, Karacabey Merino, Ramlic, German Black-Head Mutton x Kivircik, Hampshire Down x Merino) to identify M (mutant) and N (normal) alleles and genotypes. Live weight (LW) and morphometric measurements were taken on the 90th, 180th, and 360th day of the study. The principal component analysis results show that four morphometric measurements (body length, BL; chest circumference, CC; chest width, CW; withers height, WH) and two body indexes (index of body weight, IBF; Baron-Crevat index, BCI) with LW could be used to the morphometric characterization. It was found that the cannon bone perimeter (CBP) was the differentiation point of CLPG genotypes. While a lower to moderate correlation was observed between CBP to CC, LW, CW, chest depth (CD), and rump width (RW) in the NN genotype, no significant correlation was observed in the MN genotype. This investigation also revealed that BCI and IBF were the two body indexes that should be adopted into breeding schemes as a performance and etiological comparison of animals, especially in marker-assisted selection schemes due to well-reflecting animal current status.