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ÇAVAŞ, TOLGA

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ÇAVAŞ

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TOLGA

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2019 - 201912020 - 20235
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    Publication
    Evaluation of cytotoxic and genotoxic potential of avobenzone and octocrylene on human skin fibroblast cells
    (Taylor & Francis Ltd, 2023-12-31) Vatan, Özgür; Sevinç, Duygu; Yılmaz Çelik, Dilek; Aurelie Allounan, A. Carine; Teksoy, Özgün; Oral, Neylan; Hüriyet, Hüzeyfe; Çavaş, Tolga; Çinkılıç, Nilüfer; VATAN, ÖZGÜR; Sevinç, Duygu; Yılmaz Çelik, Dilek; Aurelie Allounan, A. Carine; Teksoy, Özgün; Oral, Neylan; Hüriyet, Hüzeyfe; ÇAVAŞ, TOLGA; ÇİNKILIÇ, NİLÜFER; Fen Edebiyat Fakültesi; Biyoloji Bölümü; 0000-0002-7687-3284; 0000-0003-1620-1918; AAH-5296-2021; ISV-0209-2023; O-7508-2015; JOX-7778-2023; JOR-9588-2023; JOL-2743-2023; EDJ-3505-2022; JOX-5573-2023; CVI-9578-2022; EQD-6980-2022
    We investigated the cytotoxic and genotoxic effects of the organic UVA filter avobenzone (AVB), and the UVB filter octocrylene (OCT), found alone or in combination in different sunscreen formulations, on normal human skin fibroblast cells (CCD-1118Sk). To achieve this purpose, we used XTT viability assay, single cell gel electrophoresis (comet) assay and reactive oxygen species (ROS) assay. For cytotoxicity, CCD-1118Sk cells were exposed to various concentrations of AVB (32-800 mu M), and OCT (130-6500 mu M). The IC50 values were 100.2 and 1390.95 mu M for AVB and OCT, respectively. IC12.5, IC25 and IC50 concentrations were chosen for genotoxicity testing. The comet assay revealed DNA damage at the two highest concentrations of AVB and all OCT concentrations. The rate of DNA damage was significantly higher than in the control groups. Co-treatment with AVB and OCT increased the level of DNA damage and intracellular oxidative stress compared with AVB and OCT treatment alone. Finally, our study showed that the UVA filter AVB, with the UVB filter OCT, may induce cytotoxic and genotoxic effects on human skin fibroblast cells.Highlights Avobenzone and octocrylene are UV filters used in sunscreen products. Healthy human skin fibroblast cell line CCD-1118Sk was used for cytotoxic and genotoxic assays. Cotreatment with avobenzone and octocrylene caused more severe DNA damage than their treatment alone. Our findings will contribute to the limited number of cytotoxicity and genotoxicity studies and lead to more detailed studies on the use of these two chemicals.
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    In vitro cytotoxic and genotoxic effects of donkey milk on lung cancer and normal cells lines
    (Czech Academy Agricultural Sciences, 2019-01-01) Akça, Çetin; YILMAZ, DİLEK; Vatan, Özgür; VATAN, ÖZGÜR; Yılmaz, Dilek; Hüriyet, Huzeyfe; Cinkılıç, Nilufer; ÇİNKILIÇ, NİLÜFER; Çavaş, Tolga; ÇAVAŞ, TOLGA; Fen Edebiyat Fakültesi; 0000-0002-7687-3284; 0000-0001-7269-8493; 0000-0002-3595-6286; 0000-0003-1620-1918; AAH-3508-2021; O-7508-2015; ISV-0209-2023; AAH-5296-2021
    In vitro cytotoxic and genotoxic effects of donkey milk on cancer (A549) and normal (BEAS-2B) lung cell lines were investigated. The XTT and WST-1 tests as well as clonogenic assays were used to evaluate cytotoxicity. The comet assay and micronucleus test were used as genotoxicity endpoints. Donkey milk showed lower cytotoxic effects against normal lung cell line BEAS-2B in comparison to the tumor cell line A549. Genotoxicity experiments revealed dose dependent increases in the frequencies of micronuclei and single stranded DNA breaks in A549 cells whereas no significant damage was observed in BEAS-2B cells. The results indicate that donkey milk has anti-proliferative and genotoxic effects on lung cancer cells at concentrations which are non-toxic to normal lung cells.
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    Evaluation of radiosensitivity and dna repair with comet test in patients with stat3-lof and znf341 deficiency
    (Wiley, 2022-09-01) Özen, Ahmet; Aydıner, E. Karakoç; Barış, S.; KILIÇ GÜLTEKİN, SARA ŞEBNEM; Kilic, Sara Şebnem; DEMİRÖZ ABAKAY, CANDAN; Çekic, Şükrü; ÇEKİÇ, ŞÜKRÜ; Huriyet, Huzeyfe; Hortoglu, Melika; BEKTAŞ HORTOĞLU, MELİKA; ÇAVAŞ, TOLGA; Çavas, Tolga; 0000-0002-9574-1842; 0000-0002-5617-8313; 0000-0001-5380-5898; 0000-0003-1620-1918; L-1933-2017; CVI-9578-2022; JBJ-7521-2023; AAH-3855-2021; AAH-3508-2021; FEK-8430-2022
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    Publication
    Increased radiosensitivity and impaired DNA repair in patients with STAT3-LOF and ZNF341 deficiency, potentially contributing to malignant transformations
    (Oxford Univ Press, 2022-05-02) Çekiç, Şükrü; Hüriyet, Hüzeyfe; Hortoğlu, Melika; Kasap, Nurhan; Özen, Ahmet; Karakoç-Aydıner, Elif; Metin, Ayşe; Ocakoğlu, Gökhan; Demiröz Abakay, Candan; Temel, Şehime G.; Özemri Sağ, Şebnem; Barış, Safa; Çavaş, Tolga; Şebnem Kılıç, Sara; ÇEKİÇ, ŞÜKRÜ; Hüriyet, Hüzeyfe; BEKTAŞ HORTOĞLU, MELİKA; OCAKOĞLU, GÖKHAN; DEMİRÖZ ABAKAY, CANDAN; TEMEL, ŞEHİME GÜLSÜN; ÖZEMRİ SAĞ, ŞEBNEM; ÇAVAŞ, TOLGA; KILIÇ GÜLTEKİN, SARA ŞEBNEM; Tıp Fakültesi; Biyoistatistik Ana Bilim Dalı; Hücre Kültürü ve Genetik Toksikoloji Laboratuvarı; 0000-0002-9574-1842 ; 0000-0002-5617-8313 ; 0000-0001-5380-5898 ; 0000-0003-1620-1918 ; L-1933-2017; CVI-9578-2022; JBJ-7521-2023; HLG-6346-2023; AAH-3855-2021; AAG-8385-2021; AAH-8355-2021; AAH-3508-2021; IMT-6140-2023
    STAT3 plays an important role in various complex and sometimes contradictory pathways such as proliferation, differentiation, migration, inflammation, and apoptosis. The transcriptional activity of the STAT3 gene is controlled by a transcription factor called ZNF341. There is insufficient data on radiation sensitivity and post-radiation DNA repair in STAT3- loss-of-function (LOF) patients. We aimed to investigate the radiosensitivity in patients with STAT3-LOF and ZNF341 deficiency. Twelve patients with STAT3-LOF and four ZNF341-deficiency patients were recruited from three clinical immunology centers in Turkey and evaluated for radiosensitivity by the Comet assay, comparing to 14 age- and sex-matched healthy controls. The tail length (TL) (mu m), percentage of DNA in the tail (TDNA%), and olive tail moment (OTM) (arbitrary units) were evaluated at the same time for baseline (spontaneous), initial (immediately after 2 Gy irradiation), and recovery (2 h after irradiation) periods by using a computerized image-analysis system, estimating DNA damage. Except for a patient with ZNF341 deficiency who developed nasal cell primitive neuroendocrine tumor and papillary thyroid cancer during the follow-up, there was no cancer in both groups. During the recovery period of irradiation, TL, TDNA%, and OTM values of healthy controls decreased rapidly toward the baseline, while these values of patients with STAT3-LOF and ZNF341 deficiency continued to increase, implying impaired DNA repair mechanisms. Increased radiosensitivity and impaired DNA repair were demonstrated in patients diagnosed with STAT3-LOF and ZNF341 deficiency, potentially explaining the susceptibility to malignant transformation.This study demonstrates for the first time that patients with STAT3-LOF and ZNF341 deficiency had increased radiation sensitivity and DNA repair defects compared with healthy controls. Our results highlight the need for close monitoring of these patients for the development of malignancy.
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    Publication
    The evaluation of radiosensitivity in patients with STAT3 deficiency
    (Springer, 2021-04-01) Çekiç, Şükrü; Hüriyet, Hüzeyfe; Hortoğlu, Melika; Barış, Safa; Metin, Ayşe; Özen, Ahmet; Aydıner, Elif Karakoç; Abakay, Candan; Çavaş, Tolga; Kılıç, Sara; ÇEKİÇ, ŞÜKRÜ; Hüriyet, Hüzeyfe; BEKTAŞ HORTOĞLU, MELİKA; DEMİRÖZ ABAKAY, CANDAN; ÇAVAŞ, TOLGA; KILIÇ GÜLTEKİN, SARA ŞEBNEM; Tıp Fakültesi; Çocuk Sağlığı ve Hastalıkları Ana Bilim Dalı; Çocuk İmmunoloji Bilim Dalı; 0000-0002-9574-1842; 0000-0001-8494-601X; 0000-0003-4150-5200; AAA-4154-2022; JBJ-7521-2023; R-6749-2017; L-1933-2017; HKN-1599-2023
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    Publication
    Cancer tendency in a patient with ZNF341 deficiency
    (Springer/Plenum Publishers, 2020-01-20) Çekiç, Şükrü; Hartberger, Julia Maria; Frey-Jakobs, Stefanie; Hüriyet, Hüzeyfe; Hortoğlu, Melika Bektaş; Neubauer, Johanna Charlotte; Karalı, Yasin; Abakay, Candan Demiröz; Saraydaroğlu, Özlem; Çavaş, Tolga; Grimbacher, Bodo; Kılıç, Sara Şebnem; ÇEKİÇ, ŞÜKRÜ; Huriyet, Huzeyfe; BEKTAŞ HORTOĞLU, MELİKA; KARALI, YASİN; DEMİRÖZ ABAKAY, CANDAN; SARAYDAROĞLU, ÖZLEM; ÇAVAŞ, TOLGA; KILIÇ GÜLTEKİN, SARA ŞEBNEM; Tıp Fakültesi; Radyasyon Onkolojisi Ana Bilim Dalı; 0000-0002-9574-1842; 0000-0003-1620-1918; 0000-0002-6897-6806; 0000-0001-8571-2581; 0000-0002-5617-8313; L-1933-2017; JBJ-7521-2023; AAH-3855-2021; AAA-4154-2022; AAH-1658-2021; AAH-9701-2021; AAH-3508-2021; CVI-9578-2022; FFS-1974-2022