Person: ÇOBANOĞLU, ÖZDEN
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ÇOBANOĞLU
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ÖZDEN
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Publication Genetic variation in the bovine myogenic determination factor 1 (g.782g> A polymorphism) and its influence on carcass traits in Turkish grey steppe cattle(TUBİTAK, 2021-01-01) Ardıçlı, Sena; Çobanoglu, Özden; ARDIÇLI, SENA; ÇOBANOĞLU, ÖZDEN; Veteriner Fakültesi; Genetik Ana Bilim Dalı; 0000-0003-2758-5945; O-3394-2019MyoD1 gene is a member of the myogenic differentiation gene family, which plays a crucial role in growth and development. There is a lack of information about the MyoD1 variants and their phenotypic influences in Turkish Grey Steppe cattle. Therefore the present study aimed to determine the genetic variability of MyoD1 g.782G>A polymorphism and to evaluate its effects on carcass characteristics in Turkish Grey Steppe cattle. Analyses were conducted on a total of 142 Turkish Grey Steppe bulls. The PCR-RFLP technique was used for genotyping of the g.782G>A polymorphism in exon 1 of the MyoD1 gene. The population genetic parameters including compatibility with Hardy-Weinberg equilibrium, heterozygosity, number of effective alleles, the polymorphism information content, and the fixation index were calculated. Statistical analysis was carried out using the least square methods of the general linear model procedure. Results revealed that BB was the preponderant genotype (41.55%) but the AA genotype exhibited a remarkable close frequency (39.44%). Accordingly, allelic frequencies were very close to each other (A:0.49; B:0.51). The chi 2 test revealed that the corresponding MyoD1 locus did not conform to Hardy-Weinberg equilibrium (p < 0.001). Heterozygosity, number of effective alleles, and the polymorphism information content values were 0.4998, 1.9992, and 0.3749, respectively. Moreover, the fixation index value was found to be 0.6195. These results indicated that MyoD1 g.782G>A polymorphism is a mildly informative genetic marker for Turkish Grey Steppe cattle. Statistical analysis indicated that the studied MyoD1 locus was significantly associated with chilling loss, carcass bone content, and carcass length (p < 0.05). The AA genotype was characterized by a higher chilling loss percentage but lower carcass length and bone content compared to BB and heterozygous genotypes. This study may provide valuable information regarding cattle carcass assessment and improvement through marker-assisted selection.Publication Influence of some environmental factors on milk yield and milk components traits in jersey cows(Parlar Scientific Publications (P S P), 2019-01-01) Kul, Ertuğrul; Gürcan, Eser Kemal; Çankaya, Soner; Abacı, Samet Hasan; Ülker, Mehmet; Çobanoğlu, Özden; ÇOBANOĞLU, ÖZDEN; Veteriner Fakültesi; 0000-0001-9633-634X; AAM-4544-2020This study was conducted to assess the effects of stage of lactation, parity and sampling season on milk yield and milk components in Jersey cows in Karakoy State Farm, Turkey. The data set consisted of 2657 milk records of 204 Jersey cows collected from September 2011 to December 2013. For this purpose, test day milk yield (TDMY) records and milk samples were taken once a month for 10-month period after parturition. The effect of stage of lactation, parity and sampling season on TDMY, fat percentage, protein percentage, fat yield and protein yield were found statistically significant (P<0.01). TDMY were correlated with fat percentage (-0.235) and protein percentage negatively (-0.254) but with fat yield (0.755) and protein yield positively (0.950) (P<0.01).In conclusion, these results suggest animal breeders that the effects of lactation stage, parity and sampling season may be taken into account in husbandry management to improve the milk yield and milk quality in Jersey cows.Publication Genetic variation at the olr1, anxa9, myf5, ltf, igf1, lgb, csn3, pit1, mbl1, cacna2d1, and abcg2 loci in Turkish Grey Steppe, Anatolian Black, and East Anatolian Red Cattle(Tubitak Scientific & Technological Research Council Turkey, 2022-01-01) ÇOBANOĞLU, ÖZDEN; ARDIÇLI, SENA; Veteriner Fakültesi; Genetik Ana Bilim Dalı; 0000-0003-2758-5945; O-3394-2019Native breeds are excellent sources of genetic variation. Anatolian native breeds are relatives of the first cattle domesticated and are ancestors of many European breeds. Therefore, this study aimed to evaluate the genetic variation of OLR1, ANXA9, MYF5, LTF, IGF1, LGB, CSN3, PIT1, MBL1, CACNA2D1, and ABCG2 markers in Turkish Grey Steppe, Anatolian Black, and East Anatolian Red cattle. The analysis included 367 cattle and the genotyping was performed by the PCR-RFLP. Population genetics indices including heterozygosity, the number of effective alleles, the polymorphism information content, fixation index, and the level of possible variability realization, and moreover, the genetic diversity parameters including Shannon???Weaver diversity index and Simpson dominance index were estimated. Hardy???Weinberg equilibrium was evaluated based on the number of individuals per genotype. Native breeds exhibited admissible population genetics and diversity levels. There was no animal with the MYF5-AA, IGF1-CC, LGB-BB, CSN3-BB, CACNA2D1- GG, and ABCG2-AC genotypes in the Anatolian Black breed. The frequencies of the genotypes/alleles favorable for milk production traits were remarkably low in all breeds. These findings could provide useful information on the genetic variation of Anatolian native cattle and the genetic investigations of resistance and health traits in bovine breeding and genetics.Publication Determination of the genetic polymorphism for DGAT1 gene in Holstein, Jersey and native cattle breeds of Turkey(Agricultural Research Communication Centre, 2019-02-01) Gürcan, E. K.; Çobanoğlu, Özden; Kul, E.; Abacı, H. S.; Çankaya, S.; ÇOBANOĞLU, ÖZDEN; Veteriner Fakültesi; Genetik Ana Bilim Dalı; AAM-4544-2020This study was to determine the genetic polymorphism in terms of the DGAT1 gene among Holstein and Jersey and indigenous Native Black, East Anatolian Red and Grey Steppe in Turkey. The frequencies of K allele for the DGAT1 gene were calculated as 0.59, 0.54, 0.53, 0.62, 0.52, and 0.54 for Jersey, Holstein (Black Sea Region), Holstein (Marmara Region), and Grey Steppe, East Anatolian Red, and Native Black breeds, respectively. The F-IS values of the populations for the DGAT1 gene were detected all negative values. In overall population, F-IS value was found as 76%. The expected deviations from the Hardy-Weinberg Equilibrium in terms of the DGAT1 locus were found significant in these six different populations (P<0.01). The genetic distance values among the populations were calculated between 0.0001 and 0.0089. Based on the cluster analysis, Holstein, Native Black and East Anatolian Red were located in close cluster; however, Jersey and Grey Steppe were grouped in different cluster.Publication Establishment of AA-NAT, SCD, PROP1, and IGFBP3 gene frequency distributions in Karacabey merino and kivircik sheep breeds(Hellenic Veterinary Medical Soc, 2023-07-01) Dinçel, Deniz; Çobanoğlu, Özden; DİNÇEL, DENİZ; ÇOBANOĞLU, ÖZDEN; Veteriner Fakültesi; Genetik Ana Bilim Dalı; JKT-4511-2023; ESN-8515-2022The aim of the current study was to investigate the genotypic dispersion of AA-NAT, SCD, PROP1, and IGFBP3 genes in the target population. Primarily, the genomic DNA was isolated from blood samples by the phenol/chloroform method. The genomic analyses were performed on a total of 160 Karacabey Merino (KM) (n=80) and Kivircik sheep (n=80). The PCR-RFLP method was used to determine the genotype of the SNPs. Deviation from Hardy-Weinberg equilibrium (HWE) and population genetic assays such as observed (Ho) and expected (He) hetero-zygosities, effective allele numbers (Ne), and the polymorphism information content (PIC) were calculated for each gene in the population. As a result, there was no deviation from HWE was determined except SCD and PROP1 gene in KM. The most frequent genotypes were found AA (66,25% and 87,50%) for AA-NAT gene, CC (93,75% and 91,25%) for SCD gene and CC genotype (70,00% and 56,25%) for PROP1 gene in KM and Kivircik sheep. Both breeds were detected monomorphic in terms of the IGFBP3 gene. In conclusion, the other regions of the IGFBP3 gene which were determined as monomorphic in the studied flock could be researched. Moreover, further studies should be necessary for defining the effects of investigated genes on related traits in KM and Kivircik sheep in terms of polymorphic genes specified.Publication Genetic Polymorphism of STAT1 and STAT5A Genes in Holstein, Jersey, and Indigenous Cattle Breeds in Turkey(Kafkas Univ, Veteriner Fakultesi Dergisi, 2020-03-01) Kul, Ertuğrul; Abacı, Samet Hasan; Gürcan, Eser Kemal; Çankaya, Soner; Çobanoğlu, Özden; ÇOBANOĞLU, ÖZDEN; Veteriner Fakültesi; Genetik Ana Bilim Dalı; 0000-0001-9633-634X; AAM-4544-2020This study aimed to determine genetic polymorphism in STAT1 and STAT5A genes for dairy cattle and some native cattle breeds in Turkey. 283 Jersey and a total of 472 Holstein cows from two different herds and 93 Grey Steppe, 85 Anatolian Black Cattle, and 66 East Anatolian Red cattle were used in this research. Generally, C allele gene frequency was higher than T allele for STAT1 in all breeds whereas C allele gene frequency was detected higher than G allele for STAT5A in Jersey and East Anatolian Red. On the other hand, G allele gene frequency was higher than C allele in Holstein, Grey Steppe, and Anatolian Black Cattle breeds. The expected deviations from the Hardy-Weinberg Equilibrium were significant only for Jersey breeds for STAT1 gene. Meanwhile, the expected deviation from equilibrium was also significantly different for Holstein in Black Sea Region (BSR), Anatolian Black Cattle and Grey Steppe for the STAT5A gene. FIS values were determined to STAT1 gene as negative for all breeds except for Holstein in Marmara Region (MR). Similarly, this value was determined to STAT5A gene as positive for all breeds except for Holstein in BSR. The genetic distances for two loci were calculated between 0.0029 and 0.1599 among all populations. Depending on the cluster analysis, Holstein in BSR and MR, Anatolian Black Cattle, East Anatolian Red were closely clustered to each other, while Grey Steppe and Jersey were located in completely different clusters. As a conclusion, based on the detected genetic diversity in STAT1 and STAT5A genes, it is possible to make a genetic improvement among bovine breeds raised in Turkey.Publication Determination of the association of ghr / alul gene polymorphisms with milk yield traits in holstein and jersey cattle raised in Turkey(Copernicus Gesellschaft Mbh, 2021-09-23) Kul, Ertugrul; Gurcan, Eser K.; Abaci, Samet H.; Cankaya, Soner; Cobanoglu, Ozden; ÇOBANOĞLU, ÖZDEN; Tıp Fakültesi; Genetik Mühendisliği Ana Bilim Dalı; 0000-0002-1341-4056; GPX-7921-2022; JFS-8031-2023; ABA-4511-2020This research was carried out to determine the effect of a specific single nucleotide polymorphism (SNP) region in exon 10 of the growth hormone receptor (GHR) gene on milk production traits in Jersey and Holstein cows raised in Turkey. Milk samples were recorded as a test day milk yield (TDMY) and an adjusted based 305 d milk yield (305-DMY). Also, milk component traits were detected. Based on the scope of this study, a total of 748 dairy cows, including 305 Holsteins raised in the Marmara Region and 163 Holstein and 280 Jersey raised in the Black Sea Region, were genotyped for the GHR gene using the RFLP-PCR technique. Jersey cows carrying the GG genotype (5.24 %) were associated with higher fat content (P < 0.05). Jersey cows with GG and AG also had a higher protein content (3.44 % and 3.38 %, respectively) (P < 0.05). Similarly, the protein content was the highest in Holstein cows with the GG genotype (3.46 %) (P < 0.01), whereas Holstein cows having AA genotypes displayed higher TDMY (24.64 kg/d) (P < 0.05) and 305-DMY (8472.4 kg) (P < 0.01). The estimated increase in milk protein and fat contents due to the G allele was 0.07 % and 0.22 % in the Jersey breed, respectively. On the other hand, allele A was highly related to an increase in protein yield and 305-DMY of 0.04 and about 675 kg in the Holstein breed, respectively. The GHR gene should be considered as a potential candidate gene in marker-assisted selection programs to improve the performance of milk and related traits in Turkey dairy cattle populations.Publication Individual identification and assessment of genetic diversity using microsatellite markers in racing pigeons raised in Turkiye(Bursa Uludağ Üniversitesi, 2024-06-05) Şentürk, Nursen; Taşkın, Sebahat Dilara; Çobanoğlu, Özden; Ardıclı, Sena; Şentürk, Nursen; Taşkın, Sebahat Dilara; ÇOBANOĞLU, ÖZDEN; ARDIÇLI, SENAThe implementation of a swift and economical molecular genetic approach, ensuring both efficacy and cost-effectiveness and facilitating population certification, is of utmost significance for breeders and the conservation of Turkiye's native pigeon biodiversity. In this study, we aimed to examine the genetic structure of racing pigeons (Columba livia domestica) raised in Turkiye using a genetic marker panel consisting of eight short tandem repeat (STR) loci. For this purpose, DNA was isolated from the shed feathers of 216 pigeons. Genomic DNA was amplified using the multiplex allele-specific PCR and subsequent capillary electrophoresis with ABI PRISM 3130XL Genetic Analyzer. Next, PCR products were analyzed in the GeneMapper Software program (Applied Biosystems). For parent testing, paternity index (PI), combined paternity index (CPI), and cumulative probability of paternity (CPP) were calculated. Furthermore, population genetic diversity was evaluated using heterozygosity (He), polymorphism information content (PIC), and Hardy–Weinberg equilibrium (HWE) testing. Results revealed that the total number of alleles is 81 and the number of alleles per locus varies between 4 and 19. The similarity rate between parent and offspring was calculated as 99.99% and above. Since no pedigree information was given when the samples were analyzed, obtaining this similarity ratio demonstrates the reliability of the panel. He values range from 0.362 to 0.919, and the PIC values range from 0.295 to 0.909. Loci PG-1, PG-2, and PG-3 show significant genetic diversity, with moderate to high PIC values reflecting varied allele frequencies in the population. Consequently, the set of seven STR markers (+ one sex marker) can be applied to identify and confirm parentage on a regular basis, thereby facilitating efficient breeding programs and ensuring genetic diversity conservation. This panel enables efficient pedigree analysis and gender determination, optimizing cost-effectiveness. The methodology presented in this study is ideal for pedigree analysis and breed certification in the Turkish pigeon breeding industry. Consequently, we affirm that the study data carries considerable national importance.