Person: SUSURLUK, İSMAİL ALPER
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SUSURLUK
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İSMAİL ALPER
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Publication Comparison of hermaphrodites of hybrid Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH strain and its parents on reproduction capacity(Ege Üniversitesi, 2021-01-01) Ulu, Tufan Can; Özbudak, Gizem; Düzenli, Elif Özge; Çakır, Şeyrna Hümeyra; Susurluk, İsmail Alper; Özbudak, Gizem; Düzenli, Elif Özge; Çakır, Şeyrna Hümeyra; SUSURLUK, İSMAİL ALPER; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Bitki Koruma Bölümü; 0000-0003-3640-1474; B-6308-2011; AAG-7131-2021; FPE-3049-2022; CNN-6967-2022Entomopathogenic nematodes (EPNs) are microscopic roundworms used in biocontrol. EPNs are obligate insect parasites, they live in soil, and they are especially effective against soilborne insects. They are a good alternative to chemical pesticides thanks to their advantages, such as prolonged longevity, broad host range and mass production suitability. However, EPNs cannot compete with chemical pesticides due to high production costs and short shelf life. The aim of this study was to determine the reproduction capacity of the Turkish hybrid Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH strain and then compare it with its parents to improve its liquid culture yield. In this way, it is aimed to reveal the effects behind the high reproduction potential of the hybrid HBH strain. All experiments were performed at Bursa Uludag University, Faculty of Agriculture, Department of Plant Protection, in 2020. All cadavers were periodically dissected, hermaphrodites were counted and their body lengths were measured. Compared to its parents, the hybrid HBH strain had greater hermaphrodite counts, with mean 66 individuals within 12 days, and hermaphrodite body length, with mean 3.88 mm. The results obtained from this study should provide information for commercial EPN production development.Publication Optimization of in vitro solid culture of Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH hybrid strain(Ege Üniversitesi, 2021-01-01) Ulu, Tufan Can; Susurluk, İsmail Alper; SUSURLUK, İSMAİL ALPER; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Bitki Koruma Bölümü.; 0000-0002-0699-1752; AAG-7131-2021Entomopathogenic nematodes are soil-dwelling biocontrol agents. EPNs need an insect host to complete their life cycle, and they kill their host during its development. The major disadvantage of EPNs is the high cost of commercial products. Thus, there are many studies focused on reducing production costs by optimization of mass production. In a previous project, Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH hybrid strain was developed from local isolates. This hybrid strain was patented due to superior bioecological characteristics. This study aimed to optimize in vitro solid mass production of hybrid strain. All laboratory trials were performed between 2017 and 2018, in Nematology Laboratory of Bursa Uludag University, Faculty of Agriculture, Department of Plant Protection. For optimization, additional supplementary ingredients (soy lecithin and egg yolk), temperature (24, 28 and 32 degrees C) and medium pH (5, 7 and 9) were selected as production parameters. Optimization was evaluated based on hermaphrodite egg numbers, total infective juveniles (IJs), IJ body length and IJ virulence. Based on results, best production combination was found as agar containing soy lecithin, 28 degrees C and pH 7. Also, agar media with pH 9 markedly reduced production yield. Consequently, optimum values for some important in vitro solid production parameters of HBH hybrid strain were determined.Publication Effects of insecticides and synergistic chemicals on the efficacy of the entomopathogenic nematode steinernema feltiae (Rhabditida: Steinernematidae) against leptinotarsa decemlineata (Coleoptera: Chrysomelidae)(Elsevier, 2021-03-14) Özdemir, Esengül; İnak, Emre; Evlice, Emre; Yüksel, Ebubekir; Delialioğlu, Rabia Albayrak; Susurluk, İsmail Alper; SUSURLUK, İSMAİL ALPER; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Bitki Koruma Bölümü; AAG-7131-2021The Colorado potato beetle (CPB), Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae), is considered as one of the most important pests of potato plants. Control of CPB is mainly based on chemical insecticides. However, control failure is often reported because of the rapid resistance development ability of CPB; therefore, there is an urgent need for integrated control methods. In this study, we evaluated the combined usage of insecticides chlorantraniliprole, spinosad, and spinetoram, which are registered for CPB control, with the entomopathogenic nematode Steinernema feltiae (Rhabditida: Steinernematidae).In addition, the efficacy of S. feltiae on a chlorantraniliprole-resistant CPB population exposed to synergistic chemicals PBO (Piperonyl butoxide), DEM (Diethyl maleate) and DEF (S,S,S-tributylphorotrithioate) inhibiting certain detoxification enzyme groups was determined. The tested insecticides showed no adverse effect on the survival and infectivity of S. feltiae and the isolate was considered compatible with integrated CPB control. This study also showed that pre-treatment with synergistic chemicals (particularly PBO and DEM) increased the efficacy of S. feltiae against CPB. To our knowledge, this is the first study determining the usage of entomopathogenic nematode + synergist combination. Therefore, further studies on the potential use of synergistic chemicals in combination with entomopathogenic nematodes should be performed under both laboratory and field conditions.Publication Biocontrol potential of Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH hybrid strain against the beet webworm, Loxostege sticticalis L., 1761 (Lepidoptera: Pyralidae)(Ege Üniversitesi, 2022-10-10) Dede, Emre; Bütüner, Alperen Kaan; Susurluk, İsmail Alper; Dede, Emre; BÜTÜNER, ALPEREN KAAN; SUSURLUK, İSMAİL ALPER; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Bitki Koruma Bölümü.; 0000-0002-2121-3529; 0000-0001-9017-9187; 0000-0002-0699-1752; GXV-0837-2022; AAG-7131-2021; HNE-4934-2023With limits on the use of pesticides, biological control has become increasingly important. Consequently, entomopathogenic nematodes (EPN) are now used widely in biological control. EPNs can potentially be used against beet webworm, Loxostege sticticalis L., 1761 (Lepidoptera: Pyralidae), which established in sunflower-growing areas in Turkiye in 2022. Therefore, the hybrid EPN strain, Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH, was assessed for this purpose. The study was conducted in Bursa Uludag University, Faculty of Agriculture, Plant Protection Department, Nematology Laboratory in 2022. Four nematode doses (2, 5, 10 and 20 IJs) were applied to the last instars of L. sticticalis at three temperatures (20, 25 and 30 degrees C). The highest mortality was 97% with 20 IJs dose nematodes at 30 degrees C. LD50 and LD90 of the nematode were determined at all tested temperatures. The lowest LD50 and LD90 were at 30 degrees C; 4.37 and 11.0 IJs, respectively. These results indicated that the HBN strain has potential for control of L. sticticalis.Publication Efficiency of temperature and storage duration on some morphological measurements and reproductive capacity of the entomopathogenic nematode Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae)'s Turkish HBH hybrid strain(Entomological Soc Turkey, Ege Univ, 2023) Bütüner, Alperen Kaan; Susurluk, İsmail Alper; BÜTÜNER, ALPEREN KAAN; SUSURLUK, İSMAİL ALPER; 0000-0002-2121-3529; GXV-0837-2022; JVR-3089-2024Entomopathogenic nematodes (EPNs) are successfully used in the biological control of agricultural insect pests. This study aims to determine the body length of hermaphrodite individuals, egg diameter and reproductive capacity obtained from Infective Juveniles (IJs) stored at different temperatures and durations. Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae)'s Hybrid Strain HBH was used in the study. IJs stored at 15, 25 and 35 degrees C for 7, 14 and 21 days were inoculated onto Galleria mellonella L., 1758 (Lepidoptera: Pyralidae) last instar larvae at a dose of 100 IJs. On the 2nd day of infection, hermaphrodite individuals and eggs were obtained by dissecting the larvae. The reproductive capacity was determined 10-12 days after infection. The study was conducted in Bursa Uludag University, Faculty of Agriculture, Plant Protection Department, Nematology Laboratory in 2023. In conclusion, the longest hermaphrodite individuals and egg diameter were obtained as 6207.22 mu m and 55.65 mu m, respectively from the IJs stored for 7 days at 15 degrees C. The highest reproductive capacity was also observed as 167.500 IJs per G. mellonella larva in IJs stored under the same conditions with respect to temperature and time. This study is important for assessing the morphological effects of different temperature values and storage durations on EPNs.