Person: ERSOY, SEMİHA
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ERSOY
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SEMİHA
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Publication The effects of fat graft and platelet-rich fibrin combination after epineurectomy in rats(Assoc Medica Brasileira, 2023-01-01) Kastamoni, Menekşe; KASTAMONİ, MENEKŞE; Yavaş, Senem Esin; Ersoy, Semiha; ERSOY, SEMİHA; Özgenel, Güzin Yeşim; ÖZGENEL, GÜZİN YEŞİM; Bursa Uludağ Üniversitesi/Tıp FakültesiOBJECTIVE: One of the most important factors that adversely affects the outcome of peripheral nerve surgery is the formation of epineural and extraneural scar tissue after surgery. Many surgical methods and pharmacological and chemical agents have been used to prevent the formation of epineural scar tissue, but satisfactory results have not been achieved in clinical applications. The purpose of this study was to investigate the combined effect of fat graft and platelet-rich fibrin on the formation of epineural scar tissue and on nerve healing in the mature rat model.METHODS: A total of 24 female Sprague-Dawley rats were used. A circumferential segment of epineurium was excised from both bilateral sciatic nerves. The epineurectomized right nerve segment was wrapped with a combination of fat graft and platelet-rich fibrin (experimental group), while the left nerve segment did not receive any surgical procedure other than the epineurectomy (sham group). Notably, 12 randomly selected rats were sacrificed in the fourth week for histopathological examination of early results. The other 12 rats were sacrificed in the eighth week for late results.RESULTS: The formation of fibrosis, inflammation, and myelin degeneration were less common in the experimental group, while nerve regeneration was found to be higher at both 4 and 8 weeks.CONCLUSION: The intraoperative application of a combination of fat graft and platelet-rich fibrin appears to be effective on nerve healing after surgery at both the early and late periods.Publication Identification of mast cells and immunophenotypic subtypes in peripheral nerve damage caused by epineurotomy and systemic inflammation(Wolters Kluwer Medknow Publications, 2023-01-01) Yavaş, Özkan; YAVAŞ, ÖZKAN; Yavaş, Senem Esin; Kavruk, Kaan; KAVRUK, KAAN; Özbek, Serhat; ÖZBEK, SERHAT; Ersoy, Semiha; ERSOY, SEMİHA; Bursa Uludağ Üniversitesi/Veteriner Fakültesi; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Plastik Cerrahi Anabilim Dalı.; KHD-4075-2024Purpose: This study aims to investigate the numerical increase, localization, granulation status, and immunophenotypic properties of mast cells (MCs) in epineurectomy-induced nerve damage and lipopolysaccharide (LPS)-induced systemic infection models. Materials and Methods: In this study, the animals were divided into three groups of 6 each. One of the groups was determined as the control group, epineurectomy was applied to one group, and systemic inflammation was created by regular LPS injections in the other group. Then, the obtained nerve tissues were stained histochemically with Hematoxylin and Eosin toluidine blue, and the increase, localization, and granulation status of MCs were examined. Immunohistochemically, antitryptase and antichymase staining were performed to determine the immunophenotypes of MCs. Results: As a result, while the number of MCs increased in both groups compared to the control group, MCs in the LPS group were in the epineurium, and MCs in the epineurotomy group were located between the nerve fibers. While MCs in the LPS group showed very severe degranulation, mild degranulation was observed in the epineurotomy group, and almost no degranulated MCs were observed in the control group. Conclusion: This study is critical because it is one of the first studies to compare MCs in different nerve damage types and examine the expression of chymase and tryptase.Publication Application of several special staining methods for paraffin sections on epon-embedded semithin sections(Duzce Univ, Fac Medicine, 2023-01-01) Yavaş, Senem Esin; BAŞAR, DORUK; Ersoy, Semiha; ERSOY, SEMİHA; Başar, Doruk; Akbas, Ayse; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.Aim: This study aimed to compare several specific staining protocols recommended for paraffin sections and toluidine blue and light green double staining combination to be tried for the first time with routine toluidine blue staining on semithin epon sections. Material and Methods: Samples of 1x1x1 mm were taken from the liver, skin, and aorta tissues of Wistar albino adult rats. Tissue samples were fixed with 5% glutaraldehyde at +4 degrees C overnight, postfixed with 1% osmium tetroxide for one hour, and then, blocked with Epon 812 after processing. Semithin sections of 1 mu m thickness were obtained from the epon blocks. Sections were stained with Altmann's method (for mitochondria), Verhoeff's method (for elastic fibers), Gordon&Sweets' silver impregnation method (for type III collagen), toluidine blue and light green double staining combination (for type I collagen) and routine toluidine blue method. Results: In liver sections, mitochondria in hepatocytes were differentiated by the Altmann method, and stromal type III collagen fibers were distinguished with Gordon&Sweets' method. Elastic lamellar structures were easily observed in black in the aortic sections stained with the Verhoeff method. Successful results were obtained in the staining of dermal type I collagen with toluidine blue and light green double staining in skin sections. Conclusion: Since the specific staining tried for the first time gave positive results in epon sections, it was concluded that these methods can be used to determine the localization of cellular and intercellular components that are aimed to be examined at the ultrastructural level.