Person: KAYGISIZ, ONUR
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KAYGISIZ
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ONUR
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Publication Investigation of VHL gene associated with miR-223 in clear cell renal cell carcinoma(Springer, 2021-11-26) Ünal, Ufuk; Çeçener, Gülşah; Ünlü, Havva Tezcan; Vuruşkan, Berna Aytaç; Erdem, Ecem Efendi; Egeli, Ünal; Nazlıoğlu, Hülya Öztürk; Kaygısız, Onur; Tunca, Berrin; Vuruşkan, Hakan; Ünal, Ufuk; ÇEÇENER, GÜLŞAH; Ünlü, Havva Tezcan; AYTAÇ VURUŞKAN, BERNA; Erdem, Ecem Efendi; EGELİ, ÜNAL; ÖZTÜRK NAZLIOĞLU, HÜLYA; KAYGISIZ, ONUR; TUNCA, BERRİN; VURUŞKAN, HAKAN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Patoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Üroloji Anabilim Dalı.; 0000-0003-4913-3616; 0000-0002-3820-424X; 0000-0002-0910-4258; 0000-0001-7904-883X; 0000-0002-9790-7295; 0000-0002-1619-6680; AAH-1420-2021; L-9439-2019; GYU-0252-2022; ABC-1357-2020; AAP-9988-2020; GYU-0252-2022; EEJ-1452-2022; ESY-2704-2022; FLN-9596-2022; ABI-6078-2020; EFH-9523-2022Background Clear cell type renal cell carcinoma (ccRCC) is the most common renal cell carcinoma (RCC). In this study, we examined the expressions of VHL and miR-223 in ccRCC patients' tissues to investigate the possible role in the development of ccRCC.Methods and results This study collected five expression profiles (GSE36139, GSE3, GSE73731, GSE40435, and GSE26032) from Gene Omnibus Data. Expressions of VHL and miR-223 in paraffinized tumor and normal tissues of 100 Turkish patients' ccRCC tissues were determined by bioinformatic data mining and real-time quantitative polymerase chain reaction (qRT-PCR). The VHL gene was subjected to mutational analysis by DNA sequencing, and pVHL was analyzed using western blotting. Our study's t-test and Pearson correlation analysis showed that VHL gene expression in tumoral tissues with a - 0.39-fold decrease was not significantly lower than normal tissues (p = 0.441), and a 0.97-fold increase miR-223 (p = 0.045) was determined by real-time PCR. Also, as a result of DNA sequence analysis performed in the VHL gene, it was found that 26% of the patients have mutations. The mutations for (VHL):c.60C>A (p.Val20=) and (VHL):c.467delA (p.Tyr156Leu) was detected for the first time in Turkish patients.Conclusions The present study demonstrated that the differences in the expression levels of miR-223 have the potential to be biomarkers to determine the poor prognosis in ccRCC.Publication Ureteral wall thickness at the stone site: A critical predictor of success and complications in children undergoing semi-rigid ureteroscopy(Elsevier, 2021-10-07) Kırlı, Elif Altınay; Bülbül, Emre; Kaygısız, Onur; Yeni, Sezgin; Can, Günay; Tutar, Onur; Onal, Bülent; KAYGISIZ, ONUR; YENİ, SEZGİN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Üroloji Anabilim Dalı.; 0000-0001-5143-6507; 0000-0002-9790-7295; L-9439-2019 ; GFT-1275-2022IntroductionWe retrospectively assessed the role of ureteral wall thickness (UWT) at the ureteral stone site in predicting the stone-free status and the complication rates in children undergoing semi-rigid ureterorenoscopy (URS).Patients and methodsThe children who underwent URS and had undergone non-contrast abdominal computerized tomography before the URS were included in the study. The following protocol was used to determine the outcome. Immediately before removing the stent, all children were evaluated by ultrasound (US) and plain film (KUB) for residual stones in the upper urinary tract and after removing the double J stent, the presence of stone fragments in the ureter was checked with URS. The children were considered stone-free if no residual fragments were identified in radiologic imaging and the evaluation of the ureter by URS. The case was accepted as a failure if any fragments were seen on immediate US, KUB, and/or during URS just after the stent removal. The patients who could not complete the standard primary URS procedure due to stone-related reasons (patients for whom we could not pass the safety guidewire behind the stone and/or the procedure was terminated due to pyuria during the procedure) were also accepted as a failure. The possible factors related to the patient, stone, ureter, and the operation that could affect the outcome and the complications following the URS were evaluated.ResultsThe children's median age was six years (1-17 years). Among the 89 children included in the study, 69 (78%) were stone-free, and 20 (22%) presented residual stone after the first URS session. The ROC analysis revealed that a UWT value of 4.5 mm (sensitivity 60%, specificity 92%) was the optimal cut-off value predictive of the URS outcome. The regression analysis revealed UWT >4.5 mm (p = 0.006) and multiple stone presentation (p = 0.005) as independent risk factors for residual stone. Complications were detected in 15 (17%) children. Thick ureteral wall (p = 0.012) and longer operative time (p = 0.016) were defined as the independent risk factors for complications.DiscussionIncreased UWT is associated with the adverse outcomes of URS due to tissue hypertrophy, edema, and mucosal bleeding may cause difficulty in removing the stone. The thick ureteral wall might increase the risk of complications due to the necessity of manipulating the instruments or the involuntary forceful use of instruments while removing the stone.ConclusionUWT was the only independent variable affecting both increased failure and complication rates in children undergoing URS.Publication Effect of operation timeline on frequency of surgical complication in deceased donor kidney transplantation(Frontiers Media SA, 2019-10-01) Ersoy, Alparslan; Düger, Hakan; Dizdar, Oğuzhan Sıtkı; Yıldız, Abdülmecit; Akgür, Suat; Kaygısız, Onur; Oruç, Ayşegul; Ünsal, Oktay; Coşkun, Burhan; Kordan, Yakup; Türker, Gürkan; Vuruşkan, Hakan; ERSOY, ALPARSLAN; Düger, Hakan; Dizdar, Oğuzhan Sıtkı; YILDIZ, ABDULMECİT; AKGÜR, SUAT; KAYGISIZ, ONUR; ORUÇ, AYŞEGÜL; Ünsal, Oktay; COŞKUN, BURHAN; Kordan, Yakup; TÜRKER, YUNUS GÜRKAN; VURUŞKAN, HAKAN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Dahiliye Anabilim Dalı/Nefroloji Bilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Dahiliye Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Üroloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Anestezi ve Reanimasyon Anabilim Dalı.; 0000-0001-5478-3192; 0000-0002-9790-7295; 0000-0002-0342-9692; 0000-0002-8242-9921; 0000-0002-9947-848X; 0000-0002-3019-581X; AAM-9726-2020; AAH-5054-2021; AAI-6642-2021; AAH-4002-2021; IZP-9361-2023; AAH-9704-2021; BBE-2157-2022; HIG-9032-2022; EJA-1761-2022; JJY-8484-2023; AAH-9704-2021; GAF-0095-2022; EFH-9523-2022Publication RNA-based markers in biopsy cores with atypical small acinar proliferation: Predictive effect of T2E fusion positivity and MMP-2 upregulation for a subsequent prostate cancer diagnosis(Wiley, 2019-02-01) Eryılmaz, I. Ezgi; Vuruşkan, Berna Aytaç; Kaygısız, Onur; Egeli, Ünal; Tunca, Berrin; Kordan, Yakup; Çeçener, Gülşah; ERYILMAZ, IŞIL EZGİ; AYTAÇ VURUŞKAN, BERNA; KAYGISIZ, ONUR; TUNCA, BERRİN; ÇEÇENER, GÜLŞAH; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Patoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Üroloji Anabilim Dalı.; 0000-0002-9790-7295; 0000-0001-7904-883X; 0000-0002-1619-6680; 0000-0002-9947-848X; 0000-0002-3820-424X; ABI-6078-2020; AAP-9988-2020; AAH-1420-2021; L-9439-2019; AAM-9726-2020; AAH-9746-2021; GWV-3548-2022Background Atypical small acinar proliferation (ASAP) is a precursor lesion of prostate cancer (PC), and PC develops from this suspicious focus or an unsampled malignant gland nearby. However, PC-related molecular alterations that could guide the timing of repeat biopsies and help monitor PC risk in ASAP-diagnosed patients have not been investigated. The purpose of this study was to first investigate the expression of seven different PC-related RNAs that included serine 2 (TMPRSS2): erythroblastosis virus E26 oncogene homolog (ERG) gene (TMPRSS2-ERG, T2E) fusion, alpha-methylacyl-CoA racemase (AMACR), kallikrein related peptidase 3 (KLK3), androgen receptor (AR), prostate cancer specific antigen 3 (PCA3), and matrix metalloproteinases (MMP)-2 and 9. Methods PC-related RNAs were evaluated using a real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) system in pathologically ASAP-diagnosed prostate biopsy cores from 55 patients presenting with a normal digital rectal examination and a PSA level of 4-10 ng/mL. Results We detected that positive T2E fusion status (P = 0.013) and the expression of AMACR (P = 0.016), AR (P = 0.016) and MMP-2 (P = 0.013) were independently and significantly associated with PC risk in ASAP patients. There were also several statistically significant correlations between expression levels. Additionally, we demonstrated that T2E fusion positive ASAP patients with higher MMP-2 expression were more likely to be diagnosed with PC at a subsequent biopsy during the follow-up period (P = 0.003). Conclusions Although, more clinical validations are needed for the stratification of PC risk in ASAP-diagnosed biopsy cores, our current results indicate that the coexistence of T2E fusion positivity with MMP-2 upregulation may help clinicians adjust their biopsy timetable and/or assessment of PC risk in ASAP-diagnosed patients with a PSA level of 4-10 ng/mL.Publication The effects of laparoscopic urologic surgery on cardiac functions: A pulse wave velocity study(Aves, 2020-07-01) Çiçek, Mehmet Çağatay; Kaynak, Yurdaer; Gunseren, Kadir Ömur; Kaygısız, Onur; Vuruşkan, Hakan; ÇİÇEK, MEHMET ÇAĞATAY; GÜNSEREN, KADİR ÖMÜR; KAYGISIZ, ONUR; VURUŞKAN, HAKAN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Üroloji Anabilim Dalı.; 0000-0002-0471-5404; 0000-0001-8673-3093; 0000-0002-9790-7295; ABC-9924-2020; L-9439-2019; HGM-5995-2022 ; EFH-9523-2022Objective: The aim of this study is to evaluate the effects of laparoscopic urologic surgery on cardiac functions by the parameter pulse wave velocity (PWV), a noninvasive method.Material and methods: Between July 2012 and February 2013, a total of 47 patients were included in this prospective controlled study. Patients who have been scheduled for laparoscopic surgery (LS) (n=30) and open surgery (n=17) were enrolled in the study. Preoperative, perioperative, and postoperative cardiovascular parameters were measured by a PWV instrument, and the results were compared between laparoscopic (L) group and open (C) group.Results: In the L group, compared to preoperative values, perioperative systolic arterial pressure, diastolic arterial pressure, and mean arterial pressure increased considerably, by 2.6%, 7.9%, and 4.7%, respectively. This was in contrary to reductions in these parameters by 9.5%, 5.7%, and 10%, respectively, in the C group. For the I. group, cardiac output (CO) and cardiac index (CI) were increased in the perioperative period and decreased in the postoperative period. For the C group, there were no changes in measurements of perioperative and postoperative CO and CI. However, these changes in CO and CI were not significantly different between the L and C groups. Postoperative large artery elasticity index decreased in both groups. However, these changes did not represent significant difference between groups.Conclusion: Compared to open surgery, LS may cause increases in perioperative blood pressures. In addition, increased blood pressures may last even on the first postoperative day. These effects may be more important for patients with high cardiovascular risk.Publication Which frequency is better for pediatric shock wave lithotripsy? Intermediate or low: A prospective randomized study(Springer, 2021-04-22) Kaygısız, Onur; Çiçek, Mehmet Çağatay; Mert, Ahmet; Akesen, Selcan; Sarandöl, Emre; Kılıçarslan, Hakan; KAYGISIZ, ONUR; ÇİÇEK, MEHMET ÇAĞATAY; AKESEN, SELCAN; SARANDÖL, EMRE; KILIÇARSLAN, HAKAN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Üroloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Anesteziyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; 0000-0002-9790-7295; 0000-0002-0471-5404; 0000-0003-4236-3646; 0000-0002-2593-7196; L-9439-2019; HGM-5995-2022; ELR-9087-2022; DXM-3644-2022; FGY-3115-2022Purpose Shock wave lithotripsy (SWL) is the first option in the treatment of pediatric kidney stones; however, optimal frequency is still uncertain. The aim of this study was to compare low frequency [60 shocks per minute (SWs/min)] and intermediate frequency [90 SWs/min] in terms of lithotripsy success, complications, cardiac arrhythmia, anesthesia time, secondary procedures, and efficiency quotient (EQ) in children. Methods Seventy-eight consecutive children who received SWL for radiopaque renal stones between July 2016 and January 2020 were randomly divided into two groups: Group 60 (SWL frequency: 60 SWs/min) and Group 90 (SWL frequency: 90 SWs/min). After exclusion (remaining 71 children), Group 60 (n = 38) and Group 90 (n = 33) were compared using univariate analysis. Results The median age of children (37 girls, 34 boys) was 5 (1-16) years. Patient demographics and stone features were similar between the groups. Success rate after the last SWL session was 81.6% (n = 31) for Group 60 and 87.9% (n = 29) for Group 90 (p = 0.527). Stone-free rate after the first, second, and third sessions was 42.1%, 18.4%, and 21.1% for Group 60 and 48.5%, 27.3%, and 12.1% for Group 90, respectively. Additional treatment rate was similar between the groups. In Group 60, the EQ was 57.83, and it was 64.07 in Group 90. Median total anesthesia time was significantly longer in Group 60 (74.5 min) than in Group 90 (32 min; p < 0.001). Conclusion Intermediate frequency and low-frequency pediatric SWL have similar success rates; however, intermediate-frequency SWL has a shorter anesthesia time.Publication The relationship between surgical complications and graft outcomes after kidney transplantation(Wiley, 2019-10-01) Düger, Hakan; Ersoy, Alparslan; Dizdar, Oğuzhan Sıtkı; Yıldız, Abdulmecit; Oruç Ayşegül; Ünsal, Oktay; Akgür, Suat; Kordan, Yakup; Kaygısız, Onur; Coşkun, Burhan; Vuruskan, Hakan; Düger, Hakan; ERSOY, ALPARSLAN; Dizdar, Oğuzhan Sıtkı; YILDIZ, ABDULMECİT; ORUÇ, AYŞEGÜL; Ünsal, Oktay; AKGÜR, SUAT; Kordan, Yakup; KAYGISIZ, ONUR; COŞKUN, BURHAN; VURUŞKAN, HAKAN; Uludağ Üniversitesi/Tıp Fakültesi/Dermatoloji Bölümü; Uludağ Üniversitesi/Tıp Fakültesi/Nefroloji Bölümü; Uludağ Üniversitesi/Tıp Fakültesi/Üroloji Bölümü; 0000-0001-5478-3192; 0000-0002-0342-9692; 0000-0002-9947-848X; 0000-0002-9790-7295; 0000-0002-8242-9921; AAM-9726-2020; AAH-5054-2021; AAH-4002-2021; AAH-9704-2021; IZP-9361-2023; JCO-5169-2023; L-9439-2019; D-6213-2013; GPK-6118-2022Publication Effects of obesity on the perioperative results and continence status in laparoscopic radical prostatectomy(Galenos Yayıncılık, 2018-01-16) Kaygısız, Onur; Özmerdiven, Cağdaş Gökhun; Vuruşkan, Berna Aytaç; Coşkun, Burhan; Günseren, Kadir Ömür; Kordan, Yakup; Yavasçaoğlu, İsmet; Vuruşkan, Hakan; KAYGISIZ, ONUR; AYTAÇ VURUŞKAN, BERNA; COŞKUN, BURHAN; GÜNSEREN, KADİR ÖMÜR; VURUŞKAN, HAKAN; YAVAŞCAOĞLU, İSMET; Uludağ Üniversitesi/Tıp Fakültesi/Üroloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı.; 0000-0002-9790-7295; AAH-9704-2021; ABC-9924-2020; AAH-9746-2021; AAM-9726-2020; JHZ-8017-2023; EFH-9523-2022Objective: To assess the effects of obesity on the surgical success and perioperative results and continence status in laparoscopic radical prostatectomy (LRP).Materials and Methods: The results of 300 consecutive patients undergoing LRP between April 2004 and January 2014 were analyzed retrospectively. Twenty patients were excluded from the study, thus, 280 patients remained. The patients were separated into 3 groups according to their body mass index. Based on this classification, group 1 (< 25 kg/m(2)) was normal, group 2 (25- 30 kg/m(2)) was overweight, and group 3 (> 30 kg/m(2)) was obese. The demographic data, intraoperative results, pathological results, and states of continence were compared among the groups.Results: There were 81 patients in group 1, 152 patients in group 2, and 47 patients in group 3. There were no significant differences when the groups were compared according to age and prostate specific antigen values. The intraoperative blood loss was high in group 3 only. Moreover, the estimated blood loss, transfusion, operative time, bilateral nerve- sparing rate, hospitalization days, and complication rate were similar between the groups. There were no significant differences when the pathological results were compared according to the positive surgical margins and Gleason scores. Although the continence rates in group 3 were significantly low 6 months after the operation (p< 0.05), the results were similar at 1 year (p= 0.738).Conclusion: LRP can be applied confidently in obese patients as well as normal and overweight patients.Publication Factors influencing lymphocele development after kidney transplant: Single center experience(Başkent Üniversitesi, 2023-03-01) Sayılar, Emel Işıktaş; Ersoy, Alparslan; Ayar, Yavuz; Aydın, Mehmet Fethullah; Şahin, Ahmet Bilgehan; Coşkun, Burhan; Kaygısız, Onur; Yıldız, Abdülmecit; Kordan, Yakup; Vuruşkan, Hakan; Sayılar, Emel Işıktaş; ERSOY, ALPARSLAN; Ayar, Yavuz; Aydın, Mehmet Fethullah; ŞAHİN, AHMET BİLGEHAN; COŞKUN, BURHAN; KAYGISIZ, ONUR; YILDIZ, ABDULMECİT; Kordan, Yakup; VURUŞKAN, HAKAN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Nefroloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/İç Hastalıkları Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Üroloji Anabilim Dalı.; 0000-0003-4607-9220; 0000-0002-5665-7402; 0000-0002-7846-0870; 0000-0002-8242-9921; 0000-0002-9790-7295; 0000-0002-9947-848X; AAJ-8220-2020; O-9948-2015; AAH-9704-2021; L-9439-2019; JCO-5169-2023; AAM-4927-2020; HYA-4372-2023; CPX-5894-2022; HIG-9032-2022; GAF-0095-2022; EFH-9523-2022Objectives: Lymphocele is a well-known postoperative surgical complication after kidney transplant. In this study, our aim was to analyze incidence, risk factors, and outcomes of posttransplant lymphocele in a large cohort.Materials and Methods: This observational study included 395 consecutive patients (219 males and 176 females) who underwent kidney transplant procedures from 183 living and 212 deceased donors in our center between January 2007 and 2014. A lymphocele was diagnosed with ultrasonography.Results: The incidence of lymphoceles in our cohort was 31.9% (n = 126). There were no significant differences with regard to body mass indexes, age of donors, deceased donor ratios, acute rejection episodes, and history of abdominal surgery between those with and without lymphoceles. The pretransplant serum albumin levels (3.29 +/- 0.67 vs 3.48 +/- 0.69 g/dL; P = .009) in the lymphocele group and diabetes mellitus ratios (15.9% vs 4.5%; P < .001) in the nonlymphocele group were lower than levels shown in the other group. The lymphocele ratio in patients who received cyclosporine was higher than that shown in patients who did not received it (37.5% vs. 27.4%; P = .032). There was no difference in lymphocele incidence between patients who were taking and those who were not taking mammalian target of rapamycin inhibitors, mycophenolate mofetil, or mycophenolate sodium. In regression analysis, presence of diabetes mellitus, transplant from deceased donors, older age of donors, and lower albumin levels were independent risk factors for posttransplant lymphocele occurrence.Conclusions: Posttransplant lymphocele was a relatively common surgical complication in our cohort. We concluded that diabetes mellitus, use of kidneys from deceased donors, older donor age, and hypoalbuminemia were independent risk factors for lymphocele development.Publication Comparison of ureteroscopic laser lithotripsy with laparoscopic ureterolithotomy for large proximal and mid-ureter stones(Karger, 2015-01-01) Kaygısız, Onur; Coşkun, Burhan; Kılıçarslan, Hakan; Kordan, Yakup; Vuruşkan, Hakan; Özmerdiven, Gökhun; Yavacaoğlu, İsmet; KAYGISIZ, ONUR; COŞKUN, BURHAN; KILIÇARSLAN, HAKAN; Kordan, Yakup; VURUŞKAN, HAKAN; Özmerdiven, Gökhun; Yavacaoğlu, İsmet; Uludağ Üniversitesi/Tıp Fakültesi/Üroloji Bölümü; 0000-0002-9790-7295; 0000-0002-8242-9921; 0000-0002-9947-848X; AAH-9704-2021; L-9439-2019; JCO-5169-2023; AAM-9726-2020; JIT-9412-2023; GAF-0095-2022; EFH-9523-2022; JIF-9579-2023; EIN-0828-2022Objectives: To compare the effectiveness and complications of ureteroscopic laser lithotripsy with laparoscopic ureter laparoscopic ureterolithotomy in mid- or proximal portion of large ureteral stones. Material and Methods: We reviewed patients with large (>15 mm) ureteral stone and those who underwent ureteroscopic laser lithotripsy CURS group) or laparoscopic ureterolithotomy (LU group). The first attempt was considered successful in patients who had residual fragments smaller than 2 mm and no conversion of the primary procedure to another. Results: Sixty patients (URS group 29, LU group 31) met inclusion criteria. FURS was used as an adjunctive procedure in one patient for URS group and in two patients for LU group in the same season. LU had a higher success rate and the first-day stone-free rate when compared with URS. Number of procedures was also significantly higher in URS group. There was no difference in stone-free rates at the first and third months, and length of hospitalization and operation were higher in the LU group. Only two patients in the LU group and one patient in the URS group had major complications. Conclusions: Laparoscopy is an effective option of large proximal and mid-ureter stone treatment; however, URS provides similar stone-free rates at three months as a minimal invasive procedure.