Person: KAHYA DEMİRBİLEK, SERPİL
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KAHYA DEMİRBİLEK
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SERPİL
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Publication Comparison of mycoplasma gallisepticum infection in different samples and ages of chicken breeder flocks(Facta-fundacio Arnco Ciencia Tecnologia Avicolas, 2020-01-01) Demirbilek, Serpil Kahya; KAHYA DEMİRBİLEK, SERPİL; Ardıçlı, Özge; ARDIÇLI, ÖZGE; Çarlı, Kamil Tayfun; CARLI, KAMİL TAYFUN; Veteriner Fakültesi; Mikrobiyoloji Ana Bilim Dalı; 0000-0001-6077-0478; 0000-0001-6045-8644; AAG-7421-2021; AAH-2842-2021This study aimed to compare method-based and newly developed sample-based methods for Mycoplasma gallisepticum (MG) detection in different samples of breeder flocks suffering from respiratory disease problems by using culture, real-time PCR (rPCR) and ELISA from chicks and embryonated eggs. Overall, 450 samples of 19-day-old chicken embryo's trachea, 450 samples of 8-day-old chicken tracheal swabs and 900 blood samples of 20-, 27-, 34-, 40- and 46-week-old breeder chickens from 5 flocks were sampled for 26 weeks, and were all tested for MG by culture, MG-rPCR and MG-ELISA. Culturing assays and rPCR were applied to 450 mixture samples from 19-day-old chicken embryo's trachea and 450 tracheal swab samples (each pooled into groups of 3) from 8-day-old chicks from the same flocks. Also, 900 blood samples from the same 5 breeder flocks suffering from respiratory disease problems were tested by MG-ELISA.In individual sample-based analyses, 55 (18.3%) of the 300 pooled swab samples were positive for MG using culture methods, and 106 (35.3%) of the same samples were found positive by rPCR (sensitivity, specificity). The ELISAs indicated that 252 (28%) of the 900 breeding blood samples were MG seropositive. Using age-based analyses, the most positive period was 46 weeks, followed by 40 weeks, 34 weeks, 27 weeks and at least 20 weeks, in order of decreasing seropositivity. When comparing the culture and rPCR results of the two different sampling methods, chicken embryo's trachea yielded more positive results than did tracheal swabs from the same flocks. In conclusion, rPCR is a highly specific, sensitive and reliable method for MG identification.Publication Investigation of videovaginoscopic, cytological and microbiological examination results of the vagina in bitches(Mezogazda Kiado Kft, 2023-11-01) Salcı, Emsal Sinem Özdemir; ÖZDEMİR SALCI, EMSAL SİNEM; Kahya-Demirbilek, Serpil; KAHYA DEMİRBİLEK, SERPİL; AAH-5294-2021Background: Different reproductive problems can be seen in bitches at any stage of reproductive life. These pathologies sometimes progress insidiously and are not noticed by the owners as the animals do not show any clinical signs; thus, detailed genital canal examinations should be performed routinely.Objectives: We aimed to investigate videovaginoscopic, cytological and microbiological examination findings of the bitches presented gynecologically healthy by their owners and to emphasize the necessity of routine gynecological examination.Materials and Methods: Totally, 30 bitches of different age and breed were examined gynecologically, and videovaginoscopy was performed following that cytological and microbiological specimens were taken. The sexual stages of bitches were determined according to videovaginoscopic and cytological findings.Results and Discussion: Videovaginoscopy revea'ed a cauli'lower-like mass suspected as transmissible venereal tumor (TVT) in a case, pink mucosal color in 28 cases and pale pink mucosal color in 2 cases. Five cases had serous discharge. Cervical tubercles were invisible in 8 cases and the longitudinal dorsomedian fold was invisible in one case. Videovaginoscopically, estrous cycle stages of intact bitches were proestrus in 2 cases, anestrus in 7 cases, diestrus in 6 and transition from estrus to diestrus in 3 cases. Cytology po'nted out the same with videovaginoscopic results. Microbiologically, Candida spp., EscheKchia coil, Proteus spp., Staphylococcus intermedius and Streptococcus aciOominimus were identified in cases in prepubertal stages. In the intact cases, Citrobacter freundii and S. schleiferi, Enterococcus faecalis, S. kloosii, S. saprophyticus we -c the other cultured microorganism. Pseudomonas aeruginosa was cultured in spayed cases. Videovaginoscopic, cytological and microbiological examinations should be routinely recommended to the owners of the bitches even though they have no genital problem, but it is important to interpret all results together. since the presence of bacteria/white blood cells does not always mean a pathological concition.Publication Effect of Mentofin application on the clearance of Mycoplasma gallisepticum (MG) from naturally infected layer chickens' trachea(Ankara Üniversitesi, 2015-01-01) Kahya, Serpil; Onat, Kaan; Erköse, Evren; Temelli, Seran; Eyigör, Ayşegül; Çarlı, Kamil Tayfun; KAHYA DEMİRBİLEK, SERPİL; Erköse, Evren; TEMELLİ, SERAN; Eyigör, Ayşegül; CARLI, KAMİL TAYFUN; Veteriner Fakültesi; Gıda Hijyeni ve Teknolojisi Ana Bilim Dalı; AAI-1092-2021; AAH-2842-2021; AAI-1101-2021; JPM-2439-2023; E-3867-2010Aim of this study was to determine if Mentofin would have any effect on Mycoplasma gallisepticum (MG) clearance from the tracheal epithelium of chickens in commercial layer flocks, which were naturally infected with MG. Results indicated that, compared to the control group, there was a significant and continuous decline in MG infection in chickens of Mentofin group determined by culture and Real-Time Polymerase Chain Reaction (MGrPCR) (P<0,05). Serology results in the control group indicated an increase in MG positivity from 25% to 40% (P>0,05), while there was no change in the Mentofin group (P>0,05). Culture results for MG positivity decreased from 85% to 5% in the Mentofin group, while this decrease was from 80% to 35% in the control group (P<0,05). There was a prominent decrease from 100% to 20% in MGrPCR positives in the Mentofin group (P<0,05) compared to a non-significant change observed from 95% to 80% in the control group (P>0,05). Results of this study indicate that Mentofin clearly had an effect on MG clearance from the tracheal epithelium, supported by detection of decline in MG infection in layers.Publication Detection of mycoplasma gallisepticum and mycoplasma synoviae by real-time pcrs and mycoplasma gallisepticum-antibody detection by an elisa in chicken breeder flocks(Kafkas Univ, Veteriner Fakultesi Dergisi, 2015-05-01) Kahya, Serpil; Yılmaz, Özge; Eyigör, Ayşegül; Temelli, Seran; Çarlı, K. Tayfun; KAHYA DEMİRBİLEK, SERPİL; Yılmaz, Özge; Eyigör, Ayşegül; TEMELLİ, SERAN; CARLI, KAMİL TAYFUN; Veteriner Fakültesi; Mikrobiyoloji Ana Bilim Dalı; 0000-0002-9415-2106; AAI-1092-2021; AAI-1101-2021; AAG-7421-2021; AAH-2842-2021; HHL-6717-2022; E-3867-2010This study aimed to determine the prevalence of Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) in breeder flocks showing respiratory symptoms. A total of 77 flocks (2153 tracheal swabs and blood samples) were sampled and all were tested by MG real time PCR (MG-rPCR) and MG-ELISA, and 32 flocks were tested by MS real time PCR (MS-rPCR). In the first part of this study covering 28 flocks, all samples from chickens with marked clinical symptoms and high MG-antibody levels gave negative results with MG-rPCR(1). Therefore, the MG-lipoprotein gene-specific primers (MG-rPCR(1)) of this PCR were replaced with MG-16S rRNA primers (MG-rPCR(2)), as were the MS-16S rRNA primers (MS-rPCR), thus the study was pursued accordingly. All of the first 28 flocks, which were 100% positive by MG-ELISA, were MG-rPCR(1) negative, whereas in the second part of the study, other 49 flocks, which were 87.8% MG seropositive, were found 42.9% positive by MG-rPCR(2). In addition, 5 selected flocks from the first 28 were negative, whereas 7.4% of the 27 selected flocks from the second 49 were positive by MS-rPCR. Overall, 81 out of 432 MG-rPCR(1-2) (18.7%) performed from 77 flocks, and 13 out of 187 MS-rPCRs (6.9%) in 32 flocks were determined as positive. ELISA results indicated that there could be significantly high false-positives in serological tests, thus results should not be relied upon one test system. Also, this study revealed that, for the confirmation of Mycoplasma-infected flocks in laboratories, rPCR is a reliable method as long as suitable primers are selected, and that MG and MS prevalence is considerably high in winter season.Publication Evaluation of fungi isolated in the veterinary microbiology laboratory in terms of human health(Agricultural Research Communication Centre, 2022-10-01) Demirbilek, S. Kahya; ARDIÇLI, ÖZGE; KAHYA DEMİRBİLEK, SERPİL; Ardıçlı, Özge; Kurnaz, Havva; Veteriner Fakültesi; Mikrobiyoloji Ana Bilim Dalı; 0000-0001-6077-0478; HOC-6049-2023; AAG-7421-2021Background: The importance of zoonotic fungal infections is increasing today and continues to increase due to changing living conditions.Methods: Between 2012 and 2019 evaluated of fungi species isolated from 51 (20.07% ) of 254 samples from 10 different animal species (dog, cat, horse, cow, goat, sheep, camel, penguin, bird, rabbit) with 10 different sample types Thus, the risk of those with zoonotic characteristics was assessed. Despite fungal hyphae and/or spores were seen in 63 (24.8%) of 254 samples in the direct microscopic examination, the isolation of fungi on Sabouraud dextrose agar occurred in only 51 of these samples (20.07%).Conclusion: Of all the 51 samples, 9 (17.64%) samples had more than one fungal agent. The predominant isolate was Aspergillus spp. with an isolation rate of 24 (47.05%), followed by Malassezia spp. 11 (21.56%), Alternaria spp. 6 (11.76%), Penicillium spp. and various yeasts 4 (7.84%), Microsporum spp. 3 (5.88%), Candida spp., Mucor spp., Geotrichum spp. 2 (3.92%) and Trichophyton spp. and Rhizopus spp. 1 (1.96%). Besides, samples were examined for the presence of bacteria and one or more of the bacteria were also isolated from 14 (27.4%) of 51 samples. This assessment in the veterinary microbiology laboratory has shown that the isolated fungi agents pose a significant risk of infection for people who take care of these animals or consume animal products and pet owners.Publication Comparison of bacterial profile and antibiotic susceptibility isolated from surgical site after ventral midline and lateral flank approaches of ovariohysterectomy in queens and bitches(TÜBİTAK, 2023-01-01) Bilen, Ebru Karakaya; Demirbilek, Serpil Kahya; Yılmaz, Öznur; Karadağ, Muhammet Ali; Aner, Havva; KAHYA DEMİRBİLEK, SERPİL; Aner, Havva; Veteriner Fakültesi; Mikrobiyoloji Ana Bilim Dalı; CNE-1191-2022; JEN-1583-2023The aim of the study was to identify the bacterial profile and antimicrobial susceptibility from surgical site cultures in queens and bitches undergoing ovariohysterectomy via ventral midline and lateral flank approaches. Healthy 22 queens and 21 bitches were assigned randomly either ventral midline or lateral flank approach for routine ovariohysterectomy. Surgical site samples were collected before the surgery and on the 1st, 2 nd,and 3 rd days after the surgery for microbiological analysis. A total of 70 different strains were isolated from 50 (29%) of 172 samples. In both queens and bitches the total number of bacteria isolated from the midline approach (n = 50) was found to be higher than in the flank approach (n = 20). The most frequently isolated bacteria were Staphylococcus intermedius group (SIG) (38.5%), followed by Enterococcus faecium (10%) and Staphylococcus lentus (8.5%). Of the isolated Staphylococcus spp. strains were positive 64% for beta-lactam resistance while 52% of that strains were also methicillin-resistant. Multidrug resistance to methicillin, beta-lactamase, and clindamycin was determined in two Staphylococcus spp. isolates. A high level of streptomycin resistance was observed in three Enterococcus faecium isolates. Overall, this study revealed that choosing the surgical site for ovariohysterectomy affected the bacterial profile and more than half of the isolates were resistant to antimicrobials. Thus, the lateral flank approach might be better than the ventral midline approach to prevent possible complications such as a surgical site infection in queens and bitches undergoing ovariohysterectomy.Publication Investigation of contagious agalactia by bacteriological and PCR methods in sheep and goats(Kafkas Üniversitesi, 2015-01-01) Göçmen, Hüban; Ülgen, Mihriban; Çarlı, K. Tayfun; Onat, Kaan; Kahya, Serpil; Özdemir, Ümit; Mat, Burak; Göçmen, Hüban; ÜLGEN, MİHRİBAN; CARLI, KAMİL TAYFUN; KAHYA DEMİRBİLEK, SERPİL; Veteriner Fakultesi; Veteriner Mikrobiyoloji Ana Bilim Dalı; 0000-0002-2245-5781; 0000-0002-6307-5179; AAG-8117-2021; B-9095-2018; AAH-2842-2021; E-3867-2010The aim of this study was diagnosis that occurrence of Contagious Agalactia by bacteriological and molecular methods in sheep and goats. A total of 339 samples from sheep and goats in Bursa, Balikesir, Canakkale and Edirne provinces were examined by bacteriological and molecular methods. The samples were 162 milk samples, 147 eye swabs, 15 joint fluids, 11 nasal swabs and 4 lung tissue. In bacteriological examination, 29 isolates were evaluated as Mycoplasma sp.. As a result of biochemical tests and growth inhibition tests, 29 (8.55%) Mycoplasma sp. were identified as 25 (7.37%) Mycoplasma agalactiae, 2 (0.58%) Mycoplasma ovipneumoniae and 2 (0.58%) Mycoplasma arginini. In molecular diagnosis, polC gene-PCR results could be detected M. agalactiae positive with 9.14% rate. As a result of this, 5 milk samples and 1 lung tissue sample were detected positive by polC-PCR while negative by bacteriological examination. The results of polC-PCR detected M. agalactiae positive with 14.19% rate of milk samples, 13.33% rate of joint fluids, 2.72% rate of eye swabs and 50% rate of lung tissue samples but nasal swabs were detected as negative. In this study, presence of Contagious Agalactia were investigated by bacteriological and molecular methods and M. agalactiae was detected as a main agent which cause disease however other Mycoplasma species which cause disease were not observed.Publication Comparison of cytological, microbiological and histopathological findings of genital tracts in cows with different degree perineal conformation disorder(Sivar-soc Italiana Veterinari Animali Reddito, 2021-02-01) Intas, Kamil Seyrek; Salci, Emsal Sinem Ozdemir; ÖZDEMİR SALCI, EMSAL SİNEM; Yavas, Ozkan; YAVAŞ, ÖZKAN; Ardicli, Ozge Yilmaz; Sonmez, Gursel; SÖNMEZ, GÜRSEL; Demirbilek, Serpil Kahya; KAHYA DEMİRBİLEK, SERPİL; Ardicli, Sena; ARDIÇLI, SENA; Veteriner Fakültesi; Mikrobiyoloji Ana Bilim Dalı; 0000-0001-6077-0478; 0000-0003-2758-5945; KHD-4075-2024; O-3394-2019; AAG-7421-2021; AAH-5294-2021The aim of this study is to investigate cytological, microbiological and histopathological findings of the genital tracts in cows with different degree perineal conformation (PC) disorder. Totally, 28 cows brought the slaughterhouse were materials of the study. Information about to age, body weight, reproductive status, number of parturitions, days in milk (DIM) and the reason for slaughtering of the cows was obtained in the anamnesis learned from the owners. Before slaughtering, cows were evaluated in terms of PC and then they were divided into four groups with equal numbers of cows according to PC disorders: group I (GRI) normal; group II (GRII) mild; group III (GRIII) moderate and group IV (GRIV) severe. PC disorders of the cows were graded on a scale in terms of vulvar angle, vulvar length over ischial arch, depth of anus and perineal length. A body condition score (BCS) was also evaluated in the cows. The perineal region and vagina were inspected for vaginal discharge and vaginal mucosal appearance, respectively. Pneumovagina was classified as negative, suspicious and positive. Sterile swab samples were taken from vagina, cervix and uterus for bacteriological culture and identification. For cytological examination, smears were taken from vagina, cervix and uterus. Tissue samples were taken from vagina, cervix and uterus for histopathological examination. Statistically, Pearson's correlation, Fisher's exact tests and regression analysis were performed for all data. Clinically, characters of the vaginal discharge (serous, foamy, mucous, purulent and urine-mixed) and vaginal hyperemia increased in GRIII and GRIV. Microbiologically, as parallel to the PC disorder, E. coli in Enterobacteriaceae family was the most common bacterium in Group III and Group IV. As PC disorder in the groups increased, cytological examination findings were found to be significant, similar to clinical and microbiological examination. However, histopathological examination gave more meaningful results in groups. Statistic results pointed out that difference between the groups in terms of vaginal mucosa color, cervical and uterine microbiology was significant. In conclusion, PC disorder in cows constitutes predisposition for many genital canal diseases that may be the cause of infertility. The presence of genital canal diseases can be suspected in cattle using the PC scale. Therefore, it is appropriate to evaluate the health status of the genital canal with other diagnostic methods (microbiology, cytology and histopathology), especially in cows with PC disorder.Publication Presence of Salmonella in retail grade a eggs determined by the International Organization for Standardization 6579 method and a LightCycler polymerase chain reaction system(Ankara Üniversitesi, 2015-01-01) Temelli, Seran; Kahya, Serpil; Ata, Zafer; Çarlı, Kamil Tayfun; Eyigör, Ayşegül; TEMELLİ, SERAN; KAHYA DEMİRBİLEK, SERPİL; Ata, Zafer; CARLI, KAMİL TAYFUN; Eyigör, Ayşegül; Veteriner Fakültesi; Mikrobiyoloji Ana Bilim Dalı; AAI-1092-2021; W-7994-2019; AAH-2842-2021; AAI-1101-2021; AAH-2842-2021This study aims to determine the presence of Salmonella in naturally contaminated grade A eggs by the standard culture method International Organization for Standardization Method 6579 (ISO) and a specific real-time PCR system (LightCycler PCR-LCPCR) to complement ISO. A total of 1635 eggs pooled into 101 samples were randomly collected within one year period from 20 different retail markets in Bursa, Turkey, carrying eggs of 16 large egg producers/suppliers of 5 cities with intensive layer production. Preparation of the egg and shell for analyses, Salmonella isolations and identifications, and detections were performed according to ISO 6887-4:2003, ISO 6579 and LCPCR, respectively. Overall Salmonella detection rate by ISO and LCPCR were 15.8 % (16/101) and 46.5 % (47/101), respectively. Out of 101 inner parts, Salmonella was detected in 11 (10.9 %) samples by ISO, and in 31 (30.7 %) samples by LCPCR. Six of 101 shell samples (5.9 %) were found to harbor Salmonella by ISO, while 18 (17.8 %) shells were positive by LCPCR. All isolates were determined as Salmonella enterica subsp. enterica serovar Enteritidis. These findings indicate considerably high Salmonella contamination in retail grade A eggs. This should be under routine monitoring by rapid methods such as PCR, complemented by standard culture to evaluate and assess the significance of risk for public health.Publication First isolation of salmonella duisburg from quail flock(Sivar-soc Italiana Veterinari Animali Reddito, 2021-06-01) ARDIÇLI, ÖZGE; ARDIÇLI, ÖZGE; KAHYA DEMİRBİLEK, SERPİL; KAHYA DEMİRBİLEK, SERPİL; Kurnaz, Havva; Carli, Kamil Tayfun; CARLI, KAMİL TAYFUN; ÇARLI, KAMİL TAYFUN; Veteriner Fakültesi; 0000-0001-6077-0478; HOC-6049-2023; AAG-7421-2021The first isolation of Salmonella enterica subsp. enterica serovar Duisburg (S. Duisburg) (4,12,[27]:d:e,n,z(15)) from quails was presented in this case report. Internal organs and ileocecal parts of intestines were collected from quails at 20-day old age in the flock (total of 150 quails) located in South Marmara region of Turkey. Isolation was performed according to International Organization for Standardization Method 6579. Regarding the identification of Salmonella-suspected colonies, API 20E test strips and Phoenix 100 ID/AST system were used. Serotyping of the isolate was undertaken using the slide serum agglutination test. Minimum inhibitory concentration results showed that Salmonella isolate was susceptible to all the tested antimicrobials. Although the prominent species is chicken in poultry, quail breeding increases its importance and extensiveness. Therefore this study may be useful not only for current antibiotic practices in quail breeding but also for further studies on avian microbiology.