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DEMİREL, SADETTİN

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DEMİREL

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SADETTİN

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2021 - 20222
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    [Pyr1]apelin-13 relaxes the rat thoracic aorta via APJ, NO, AMPK, and potassium channels
    (Aepress Sro, 2021-01-01) Şahintürk, Serdar; Demirel, Sadettin; Özyener, Fadıl; İşbil, Naciye; ŞAHİNTÜRK, SERDAR; DEMİREL, SADETTİN; ÖZYENER, FADIL; İŞBİL, NACİYE; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Anabilim Dalı; 0000-0002-7612-0055; 0000-0002-3629-5344; 0000-0002-4606-6596; AAH-3460-2021; ACQ-9887-2022; AAH-1641-2021; FBW-7104-2022
    In this study, the effect and effect mechanisms of [Pyr1]apelin-13, the dominant apelin isoform in the human cardiovascular tissues and human plasma, on vascular contractility were investigated. The vascular rings obtained from the thoracic aortas of the male Wistar Albino rats were placed in the isolated tissue bath system. After the equilibration period, [Pyr1]apelin-13 (10(-9) to 10(-6) M) was applied cumulatively to the aortic rings pre-contracted with phenylephrine in the plateau phase. The protocol was repeated in the presence of specific signaling pathway inhibitors (F13A, L-NAME, dorsomorphin, TEA, U0126, or indomethacin) to determine the effect mechanisms of [Pyr1]apelin-13. [Pyr1]apelin-13 induced a dose-dependent relaxation in the pre-contracted aortic rings. APJ, eNOS, AMPK, and potassium channel inhibition statistically significantly decreased the vasodilator effect of [Pyr1]apelin-13. MAPK and COX inhibition didn't statistically significantly changed the vasodilator effect of [Pyr1]apelin-13. In conclusion, [Pyr1]apelin-13 relaxes the rat thoracic aorta via APJ, NO, AMPK, and potassium channels.
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    Physiological role of K+ channels in irisin-induced vasodilation in rat thoracic aorta
    (Elsevier Science, 2022-01) Demirel, Sadettin; Şahinturk, Serdar; İşbil, Naciye; Özyener, Fadıl; DEMİREL, SADETTİN; ŞAHİNTÜRK, SERDAR; İŞBİL, NACİYE; ÖZYENER, FADIL; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Bölümü; 0000-0002-7612-0055; 0000-0002-4606-6596; 0000-0002-3629-5344; JAQ-7571-2023; ACQ-9887-2022; FBW-7104-2022; AAH-1641-2021
    Irisin, an exercise-induced myokine, has been shown to have a peripheral vasodilator effect. However, little is known about the mechanisms underlying its effects. In this study, it was aimed to investigate the vasoactive effects of irisin on rat thoracic aorta, and the hypothesis that voltage-gated potassium (KV) channels, ATPsensitive potassium (KATP) channels, small-conductance calcium-activated potassium (SKCa) channels, largeconductance calcium-activated potassium (BKCa) channels, intermediate-conductance calcium-activated potassium (IKCa) channels, inward rectifier potassium (Kir) channels, and two-pore domain potassium (K2P) channels may have roles in these effects. Isometric contraction-relaxation responses of isolated thoracic aorta rings were measured with an organ bath model. The steady contraction was induced with both 10-5 M phenylephrine and 45 mM KCl, and then the concentration-dependent responses of irisin (10-9-10-6 M) were examined. Irisin exerted the vasorelaxant effects in both endothelium-intact and -denuded aortic rings at concentrations of 10-8, 10-7, and 10-6 M (p < 0.001). Besides, KV channel blocker 4-aminopyridine, KATP channel blocker glibenclamide, SKCa channel blocker apamin, BKCa channel blockers tetraethylammonium and iberiotoxin, IKCa channel blocker TRAM-34, and Kir channel blocker barium chloride incubations significantly inhibited the irisin-induced relaxation responses. However, incubation of K2P TASK-1 channel blocker anandamide did not cause a significant decrease in the relaxation responses of irisin. In conclusion, the first physiological findings were obtained regarding the functional relaxing effects of irisin in rat thoracic aorta. Furthermore, this study is the first to report that irisin-induced relaxation responses are associated with the activity of KV, KATP, SKCa, BKCa, IKCa, and Kir channels.