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YILMAZTEPE ORAL, ARZU

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YILMAZTEPE ORAL

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    Publication
    Unfolded protein response is involved in trans-platinum (ii) complex-induced apoptosis in prostate cancer cells via ros accumulation
    (Bentham Science Publ Ltd, 2019-01-01) Karakaş, Didem; Cevatemre, Buse; Oral, Arzu Y.; YILMAZTEPE ORAL, ARZU; Yılmaz, Veysel T.; YILMAZ, VEYSEL TURAN; Ulukaya, Engin; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; Bursa Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Kimya Bölümü.; 0000-0002-3781-6834; 0000-0002-8962-9758; 0000-0002-2849-3332; AHD-2050-2022; KMA-2321-2024; A-5841-2017; L-7238-2018; L-6682-2018
    Background: Prostate cancer is one of the most common cancer types and it is the sixth leading cause of cancer-related death in men worldwide. Even though novel treatment modalities have been developed, it still a lifethreatening disease. Therefore novel compounds are needed to improve the overall survival.Methods: In our study, it was aimed to evaluate the anti-cancer activity of newly synthesized Platinum (II) [Pt(II)] complex on DU145, LNCaP and PC-3 prostate cancer cell lines. The cytotoxic activity of Pt(II) complex was tested by SRB and ATP cell viability assays. To detect the mode of cell death; fluorescent staining, flow cytometry and western blot analyses were performed.Results: The Pt(II) complex treatment resulted in a decrease in cell viability and increasing levels of apoptotic markers (pyknotic nuclei, annexin-V, caspase 3/7 activity) and a decrease in mitochondrial membrane potential in a dose dependent manner. Among cell types, tested PC-3 cells were found to be more sensitive to Pt(II) complex, demonstrating elevation of DNA damage in this cell line. In addition, Pt(II) complex induced Endoplasmic Reticulum (ER) stress by triggering ROS generation. More importantly, pre-treatment with NAC alleviated Pt(II) complex-mediated ER stress and cell death in PC-3.Conclusion: These findings suggest an upstream role of ROS production in Pt(II) complex-induced ER stressmediated apoptotic cell death. Considering the ROS-mediated apoptosis inducing the effect of Pt(II) complex, it warrants further evaluation as a novel metal-containing anticancer drug candidate.
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    Publication
    Impact of preventive actions on rejection rates in the preanalytical period
    (Walter De Gruyter Gmbh, 2020-02-01) Odabaşı, Merve Sena; Dirican, Melahat; Oral, Arzu Yılmaztepe; YILMAZTEPE ORAL, ARZU; Özkaya, Guven; ÖZKAYA, GÜVEN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; 0000-0003-3774-4241; 0000-0002-8962-9758; 0000-0003-0297-846X; AAG-6985-2021; A-5841-2017; A-4421-2016
    Background: It is responsibility of medical laboratories to determine and reject nonconforming samples as well as take preventive actions. In this study, we examined reasons and percentages of rejected samples. We also investigated impact of the preventive actions on decreasing the rejection rates.Materials and methods: Reasons for rejection were determined by Pareto analysis. Sigma analysis was used for each month to evaluate the ratios and compare with other studies. Some preventive actions were taken to reduce the rejection rates. Pearson's chi square test was used to evaluate effects of preventive actions. Significance level was determined as p <0.05.Results: Most of the rejected samples consisted of samples not received by the laboratory, haemolysed and insufficient samples. The percentages of samples not received by the laboratory and insufficient samples were reduced from 3.80% to 1.94% and 0.33% to 0.31% respectively, while haemolysed samples percentage was increased from 2.83% to 3.37% after the improvement actions. Also, sigma levels for samples not received by the laboratory and haemolysed samples were at the minimum while insufficient samples were at a reasonable level.Conclusion: Improvement actions achieved statistically significant decreases for samples not received by the laboratories for a long-term.