Person: ÇAKIR, CİHAN
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ÇAKIR
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CİHAN
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Publication Expression of the ionotropic glutamate receptors on neuronostatin neurons in the periventricular nucleus of the hypothalamus(Via Medica, 2022-01-01) Kocoğlu, S. Serter; Çakir, Cihan; ÇAKIR, CİHAN; Eyigör, Özhan; MİNBAY, FATMA ZEHRA; EYİGÖR, ÖZHAN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.; 0000-0002-8332-7353; 0000-0003-3463-7483; ABC-1475-2020; ABE-5128-2020; AAH-5249-2021; ABQ-8779-2022Background: Neuronostatin, a newly identified peptide, is accepted as an anorex- igenic peptide since it suppresses food intake when given intracerebroventricularly. Although the effect mechanisms of neuronostatin have been shown in different studies, there are no reports in the literature describing the mechanisms controlling neuronostatin neurons. In this study, we aimed to determine the presence of the ionotropic glutamate receptor subunits (iGluRs) in neuronostatin neurons in the periventricular nucleus of the hypothalamus. Materials and methods: The presence of glutamate receptors in neuronostatin neurons was investigated by dual immunohistochemistry. Immunohistochemistry was performed on 40 mu m thick coronal brain sections with antibodies against AMPA (GluA1-4), kainate (GluK1/2/3, and GluK5), and NMDA (GluN1 and GluN2A) receptor subunits. Results: The results showed that the neuronostatin neurons expressed most of the NMDA and non-NMDA receptor subunits. The neuronostatin neurons in the anterior hypothalamic periventricular nucleus were particularly immunopositive for GluA1, GluA4, GluK1/2/3, GluK5 and GluN1 antibodies. No expression was observed for GluA2, GluA3 and GluN2A antibodies. Conclusions: For the first time in the literature, our study demonstrated that the neuronostatin neurons express glutamate receptor subunits which may form homomeric or heteromeric functional receptor complexes. Taken together, these results suggest that multiple subunits of iGluRs are responsible for glutamate transmission on neuronostatin neurons in the anterior hypothalamic periventricular nucleus.Publication Fertilisation and early embryonic development of immature and rescue in vitro-matured sibling oocytes(Taylor & Francis, 2020-01-16) Avcı, Berrin; Kasapoğlu, Işıl; Çakir, Cihan; Özbay, Aysun; Ata, Barış; Uncu, Gürkan; AVCI, BERRİN; KASAPOĞLU, IŞIL; ÇAKIR, CİHAN; Özbay, Aysun; UNCU, GÜRKAN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; 0000-0002-8332-7353; 0000-0001-8135-5468; AAT-3479-2021; GQR-0770-2022; AAH-5249-2021; CXJ-7203-2022 ; ABE-6685-2020The objective of this study was to assess the effect of rescue in vitro maturation and immediate intracytoplasmic sperm injection (ICSI) application on fertilisation success and early embryonic development of metaphase I (MI) oocytes. This was a retrospective cohort study including 2425 sibling oocytes in 259 ICSI cycles. ICSI was performed on 104 GV (germinal vesicle) oocytes which had reached the metaphase II (MII) stage (Group 1) and 231 MI oocytes which had reached the MII stage (Group 2) following IVM (in vitro maturation). Immediate ICSI was applied following oocyte aspiration on 292 MI stage (Group 3) and 1798 MII stage oocytes (Group 4). Normal fertilisation rates in Groups 1, 2, 3 and 4 were 51.9%, 39%, 30.1% and 59.5%, respectively. The rates of blastocyst development per oocyte and per zygote were calculated as 3.8%, 3.0%, 6.8%, 14.1% and 7.4%, 7.7%, 22.7%, 23.6% for Groups 1, 2, 3 and 4, respectively. The blastocyst development rate was significantly higher in the MI-ICSI group compared with other immature oocytes. Even though performing ICSI on the oocytes at the MI stage on the day of oocyte aspiration resulted in lower fertilisation rates, it was associated with significantly higher rates of blastocyst development.Publication Evaluation of endometrioma pathophysiology and related ovarian damage by PTEN / AKT apoptosis signaling pathway(Elsevier Science, 2020-09-01) Şen, Furkan; Aslan, Kiper; Kuşpınar, Göktan; Çakır, Cihan; Kasapoglu, Işıl; Avcı, Berrin; Uncu, Gürkan; ŞEN, HAMZA FURKAN; ASLAN, MÜNİR KİPER; KUŞPINAR, GÖKTAN; ÇAKIR, CİHAN; KASAPOĞLU, IŞIL; AVCI, BERRİN; UNCU, GÜRKAN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi.; 0000-0002-9277-7735; 0000-0002-8332-7353; AAH-5119-2021; AAT-3479-2021; HTQ-5866-2023; AER-7173-2022; AAH-9694-2021; AAH-5249-2021; KEU-2073-2024; CXJ-7203-2022; ELU-2357-2022Publication The kisspeptin and kisspeptin receptor in follicular microenvironment: Is that really necessary for oocyte maturation and fertilisation?(Taylor & Francis Inc, 2022-08-19) Kuşpinar, Göktan; KUŞPINAR, GÖKTAN; Çakır, Cihan; ORAL, HALUK BARBAROS; ÇAKIR, CİHAN; KASAPOĞLU, IŞIL; Sarıbal, Seda; BUDAK, FERAH; Budak, Ferah; Avcı, Berrin; Uncu, Gürkan; UNCU, GÜRKAN; AVCI, BERRİN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/İmmunoloji Anabilim Dalı.; 0000-0002-8332-7353; 0000-0001-7625-9148; IZP-9398-2023; AAH-5249-2021; F-4657-2014; HTQ-5866-2023The aim of this study was to determine whether Kisspeptin and Kisspeptin receptor in the follicular microenvironment is necessary for human oocyte maturation and fertilisation. The cumulus cell (CC) and follicle fluids (FF) obtained from the first aspirated follicles (n = 52) from 32 patients were divided into three groups considering nuclear maturation and fertilisation results of oocytes: (1) Metaphase I or germinal vesicle stage oocytes (incomplete nuclear maturation, n = 10), (2) unfertilised metaphase II oocytes (incomplete cytoplasmic maturation, n = 16), and (3) fertilised metaphase II oocytes (completed nuclear-cytoplasmic maturation, n = 26). The gene expression levels were assessed by RT-PCR. The levels of Kisspeptin (KISS1) and Kisspeptin receptor (KISS1R) were measured by ELISA. There were no significant efficacy KISS1 and KISS1R gene expressions in cumulus cells in terms of oocyte nuclear maturation stage (Group 1, vs Group 2 + Group 3) (respectively p = .49; p = .45). In terms of the cytoplasmic maturation stage (Group 2, vs Group 3); KISS1 and KISS1R expressions in CCs were comparable (respectively p = .07; p = .08). In FFs, KISS1 and KISS1R concentrations were similar between all groups (respectively p = .86; p = .26). In conclusion, the relative KISS1 and KISS1R expressions in CC and also KISS1 and KISS1R level of FF were independent of oocytes nuclear and/or cytoplasmic maturation. Impact statement What is already known on this subject? It has been demonstrated that Kisspeptin is an essential regulator of reproductive function and plays a key role in the modulation of GnRH secretion and gonadotropin release. Still, no information is available about the link between gene expression or concentration in the follicular microenvironment and oocyte development. What do the results of this study add? The study has shown that the relative Kisspeptin (KISS1) and Kisspeptin receptor (KISS1R) and expressions in cumulus cell (CC) and also KISS1 and KISS1R levels of follicle fluids (FF) were independent of oocytes nuclear and/or cytoplasmic maturation. What are the implications of these findings for clinical practice and/or further research? Based on the findings, it is difficult to establish a concept that kisspeptin can directly induce oocyte maturation. Nevertheless, to confirm these findings, further studies with a larger sample size are needed.Publication Luteal oestradiol for patients with serum oestradiol levels lower than expected per oocyte(Taylor, 2021-03-15) Kasapoğlu, Işıl; Düzok, Nergis; Şen, Esra; Çakır, Cihan; Avcı, Berrin; Uncu, Gürkan; KASAPOĞLU, IŞIL; DÜZOK, NERGİS; Şen, Esra; ÇAKIR, CİHAN; AVCI, BERRİN; UNCU, GÜRKAN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı; 0000-0002-8332-7353; AAT-3479-2021; AAH-5249-2021; CXJ-7203-2022; JLC-5688-2023; FVL-9509-2022; ELU-2357-2022Although the efficiency of progesterone in providing luteal phase support has been established, the role of oestradiol supplementation during the luteal phase remains controversial. We evaluated pregnancy outcomes of patients who had a ratio of serum E2 levels on the hCG day to the number of oocytes retrieved (oestradiol/oocyte ratio - EOR) levels of <100 pg/ml by supporting them with additional oestradiol during the luteal phase. In total, 150 patients with an EOR < 100 pg/ml of oestradiol undergoing antagonist intracytoplasmic sperm injection (ICSI) cycles were randomly assigned into two groups to receive either oral oestradiol (4 mg/d) plus vaginal progesterone (90 mg, 2 x 1/day) (group 1) or vaginal progesterone (90 mg, 2 x 1/d) alone (group 2). Implantation rate following transfer of a single embryo did not differ between the two groups (group 1 = 33.3%; group 2 = 34.9%; p = 0.85). Similarly, both groups gave comparable clinical pregnancy rates per embryo transfer with 31.7% in group 1 compared with 28.6% in group 2 (p = 0.69). In conclusion the study suggests that the addition of 4 mg oral E2 to progesterone does not increase the probability of pregnancy.Publication Low molecular weight heparin-aspirin-prednisolone combination does not increase the live birth rate in recurrent implantation failure: A retrospective cohort study(Springer Heidelberg, 2023-05-30) Aslan, Kiper; Kasapoğlu, Işıl; Çınar, Ceren; Çakır, Cihan; Avcı, Berrin; Uncu, Gürkan; ASLAN, MÜNİR KİPER; KASAPOĞLU, IŞIL; ÇINAR, CEREN; ÇAKIR, CİHAN; AVCI, BERRİN; UNCU, GÜRKAN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.; 0000-0002-9277-7735; 0000-0002-8332-7353; AER-7173-2022; CXJ-7203-2022; IUD-1217-2023; AAH-5249-2021; ELU-2357-2022; AAT-3479-2021This study investigates the triple combination of adjuvants (low molecular weight heparin (LMWH)-aspirin-prednisolone) whether it improves the live birth rates of IVF&ICSI patients with previous implantation failure. This retrospective study included 1095 patients with >2 failed either fresh or frozen single embryo transfer cycles between 2014 Jan and 2021 Jan. Patients were divided into two subgroups. Group A consisted of patients with only vaginal progesterone for luteal phase support. Group B consisted of patients with triple (daily subcutaneous LMWH, daily 150 mg aspirin, and daily 16 mg prednisolone) luteal phase supplementation to vaginal progesterone. Demographic parameters, cycle characteristics, embryology, and pregnancy outcomes were compared, and the study's primary outcome was the live birth rate. Demographic parameters were similar between the groups. Positive b-hCG, miscarriage, and live birth rates were similar between groups as Group A vs. Group B, positive b-hCG 30.8% (190/617) vs. 35.4% (169/478), miscarriage rates 4.4% (27/617) vs. 6.7% (32/478), and live birth rates 20.4% (126/617) vs. 23.8% (114/478), respectively. When patients were stratified according to previous failures, live birth rates were still similar. Pregnancy outcomes were significantly improved in only patients with diminished ovarian reserve (Group A vs. Group B, positive b-hCG 24.2% vs. 34.3%, live birth rate 12.1% vs. 21.9%, p < 0.01). Whether the embryo transfer was fresh or frozen-thawed did not affect the results. A combined supplementation of LMWH, aspirin, and prednisolone in the luteal phase does not improve live birth rates of IVF&ICSI patients with previous implantation failure except potentially for patients with diminished ovarian reserve.Publication Comparison of ICSI outcomes with hypo-osmotic swelling (HOS) reactive sperm and motile sperm on sibling oocytes in patients with severe asthenospermia(Oxford Univ Press, 2018-07-01) Çakır, C.; Kasapoğlu, Işıl; Sarıbal, Seda; Uncu, Gürkan; Avcı, Berrin; ÇAKIR, CİHAN; KASAPOĞLU, IŞIL; Sarıbal, Seda; UNCU, GÜRKAN; AVCI, BERRİN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları ve Doğum Üreme Biyolojisi; ABE-6685-2020; AAT-3479-2021; JMI-6129-2023; CXJ-7203-2022; FTW-2214-2022Publication What is the effect of embryo morphology on serum β-hCG levels?(Elsevier, 2019-02-01) Kuşpınar, Göktan; Kasapoğlu, Işıl; Çakır, Cihan; Ata, Barış; Uncu, Gürkan; Avcı, Berrin; KUŞPINAR, GÖKTAN; KASAPOĞLU, IŞIL; ÇAKIR, CİHAN; UNCU, GÜRKAN; AVCI, BERRİN; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; 0000-0002-8332-7353; 0000-0002-0338-8368; HTQ-5866-2023; ABE-6685-2020; AAT-3479-2021; AAH-5249-2021; CXJ-7203-2022 ; AAH-5119-2021Objective: To determine the effect of embryonic factors on serum beta human chorionic gonadotropin (beta-hCG) levels in pregnancy and live birth resulting after a single fresh cleavage embryo and blastocyst transfer.Study design: This was a retrospective cohort study conducted at a tertiary care hospital. All fresh single embryo transfers (sETs) between September 2011 and December 2016 were included. The correlation analysis was performed to determine the association of embryo morphological parameters on mean serum beta-hCG levels on day 12 after the transfer of a fresh single cleavage embryo and a fresh single blastocyst embryo.Results: Out of a total of 455 fresh sETs, 60 positive beta-hCG results after the transfer of a single fresh cleavage-stage embryo and 82 after the transfer of a single fresh blastocyst. The mean beta-hCG level resulting from a single fresh blastocyst ET was 371.7 +/- 52.7 IU/L, which was similar to the mean beta-hCG level resulting from a cleavage ET (314.5 +/- 36.9 IU/L) (p=.70). Interestingly, serum beta-hCG levels resulting from a single fresh blastocyst ET showed a correlation with day 5 blastocoele expansion, trophectoderm cell number and blastocyst quality score in ongoing pregnancy (r = .33, p = .02; r = .29, p = .04; and r = .31, p = .03, respectively). Moreover, day 5 blastocoele expansion and blastocyst quality score showed a correlation with the serum beta-hCG levels resulting from a single fresh blastocyst ET in live birth (r = .36, p = .02; r = .31, p = .04, respectively).Conclusion: Our study suggests that serum beta-hCG levels resulting from a single fresh blastocyst ET showed a correlation with day 5 blastocoele expansion and blastocyst quality score in both ongoing pregnancy and live birth. (C) 2018 Elsevier B.V. All rights reserved.Publication The effects of endometriosis on oocyte morphology and embryo development(Oxford Univ Press, 2022-07-01) Avcı, Berrin; Kuspınar, Göktan; KUŞPINAR, GÖKTAN; UNCU, GÜRKAN; Çakır, Cihan; Aslan, Kiper; ASLAN, MÜNİR KİPER; Uncu, Gürkan; Kasapoğlu, Işıl; AVCI, BERRİN; ÇAKIR, CİHAN; KASAPOĞLU, IŞIL; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.; 0000-0002-8332-7353; 0000-0002-9277-7735; HTQ-5866-2023; AER-7173-2022; AAH-5249-2021