Person: ARDIÇLI, SENA
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ARDIÇLI
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SENA
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Publication Evaluation of some environmental and genetic factors csn3 and agpat6 gene on milk yield and composition in saanen goats(Univ Zagreb Vet Faculty, 2021-01-01) Dincel, Deniz; DİNÇEL, DENİZ; Ardicli, Sena; ARDIÇLI, SENA; Samli, Hale; ŞAMLI, HALE; Ogan, M. Mustafa; Balci, Faruk; BALCI, ÖMER FARUK; Bursa Uludağ Üniversitesi/Veteriner Fakültesi.; 0000-0002-8015-9032; 0000-0003-2758-5945; 0000-0003-2382-1330; AAH-6192-2021; O-3394-2019This study was designed to determine the effect of some environmental factors on milk yield and composition in Saanen goats and the effect of CSN3 and AGPAT6 gene polymorphisms on milk production traits. Saanen goats were genotyped for milk traits, and the samples were collected during the lactation, and the milk yield/compositions of each goat were specified. In terms of the CSN3 and AGPAT6 genes, the genotypes were defined by PCR-RFLP. The milk yield (MY), lactation length (LL), protein, fat, total solid (TS), solid not fat (SNF), casein, lactose rates and yields were as follows; 388.9 +/- 17.5 kg, 243 +/- 5.81 days, 3.29 +/- 0.05%, 3.59 +/- 0.07%, 11.60 +/- 0.13%, 8.43 +/- 0.06%, 2.57 +/- 0.04%, 4.31 +/- 0.03%, 11.21 +/- 0.47 kg, 12.79 +/- 0.64 kg, 40.95 +/- 1.82 kg, 29.53 +/- 1.31 kg, 8.82 +/- 0.37 kg, 15.40 +/- 0.74 kg respectively. The determined genotype and allele frequencies of CSN3 and AGPAT6 gene were as follows; FF (%99.20), MF (%0.80), F (0.996), M (0.004); GG (%12), GC (%43.20), CC (%44.80), G (0.336), C (0.664). The impacts of lactation length on all milk yield parameters (protein, fat, TS, SNF, casein, lactose. MY) (P<0.001); the age on all milk yield parameters (P<0.001) and the protein, fat, SNF, casein rates (P<0.05), the TS rate and LL (P<0.01) were found to be significant. The birth type was not found to significantly affect any milk yield parameters. Although Saanen goats showed the variation for the AGPAT6 gene (g.9263C>G), the herd was found to be monomorphic (FF) for the CSN3 gene. The effect of the AGPAT6 gene on milk traits in Saanen goats was determined not to be statistically significant. Environmental factors, such as maternal age and lactation length, were found to significantly affect some milk traits in Saanen goats. So consideration of factors such as maternal age or lactation length could be useful for improving breeding strategies for dairy goats.Publication Genetic variation in the bovine myogenic determination factor 1 (g.782g> A polymorphism) and its influence on carcass traits in Turkish grey steppe cattle(TUBİTAK, 2021-01-01) Ardıçlı, Sena; Çobanoglu, Özden; ARDIÇLI, SENA; ÇOBANOĞLU, ÖZDEN; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı.; 0000-0003-2758-5945; O-3394-2019MyoD1 gene is a member of the myogenic differentiation gene family, which plays a crucial role in growth and development. There is a lack of information about the MyoD1 variants and their phenotypic influences in Turkish Grey Steppe cattle. Therefore the present study aimed to determine the genetic variability of MyoD1 g.782G>A polymorphism and to evaluate its effects on carcass characteristics in Turkish Grey Steppe cattle. Analyses were conducted on a total of 142 Turkish Grey Steppe bulls. The PCR-RFLP technique was used for genotyping of the g.782G>A polymorphism in exon 1 of the MyoD1 gene. The population genetic parameters including compatibility with Hardy-Weinberg equilibrium, heterozygosity, number of effective alleles, the polymorphism information content, and the fixation index were calculated. Statistical analysis was carried out using the least square methods of the general linear model procedure. Results revealed that BB was the preponderant genotype (41.55%) but the AA genotype exhibited a remarkable close frequency (39.44%). Accordingly, allelic frequencies were very close to each other (A:0.49; B:0.51). The chi 2 test revealed that the corresponding MyoD1 locus did not conform to Hardy-Weinberg equilibrium (p < 0.001). Heterozygosity, number of effective alleles, and the polymorphism information content values were 0.4998, 1.9992, and 0.3749, respectively. Moreover, the fixation index value was found to be 0.6195. These results indicated that MyoD1 g.782G>A polymorphism is a mildly informative genetic marker for Turkish Grey Steppe cattle. Statistical analysis indicated that the studied MyoD1 locus was significantly associated with chilling loss, carcass bone content, and carcass length (p < 0.05). The AA genotype was characterized by a higher chilling loss percentage but lower carcass length and bone content compared to BB and heterozygous genotypes. This study may provide valuable information regarding cattle carcass assessment and improvement through marker-assisted selection.Publication Gender determination by PCR assay for the sex-determining region Y(SRY) gene amplification in linnaeus’s two-toed sloth (Choloepus Didactylus)(Bursa Uludağ Üniversitesi, 2023-06-15) Bozkurt, Berkay; Vatansever, Ezgi; ARDIÇLI, SENA; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı; 0000-0003-2758-5945In Linnaeus’s two-toed sloths (Choloepus didactylus), there is no distinct sexual dimorphism. It is an obstacle for gender determination fromthe external genitalia, especially in newborns or young sloths. Hence, easy, rapid, and reliable genetics-based methods for gender identificationof the sloths are needed to continue captive breeding more successfully. In this study, a PCR-based technique that allows gender determinationof two-toed sloths by using a sex-determining region Y (SRY) gene marker was described. The hair samples from young (suspectgender) and adult sloths (known gender) were used in genetic analysis. Initially, genomic DNA was isolated from hair root samples using Roche high pure PCR template preparation kit. The SRY primers were specifically designed based on the NCBI and Ensembl databases, andthey were verified with the BLAST program concerning the two-toed sloth genome. PCR amplification with the SRY-specific primers wascarried out by a programmable thermal cycler device using FastStart High Fidelity PCR System, Roche dNTPack. The samples were then electrophoresedon 2% agarose gels and were visualized by a gel documentation and analysis system. A specific band in the electrophoresis patternis diagnostic for a male individual with a partial SRY region. Hence, the analysis demonstrated that the samples belonged to a male two-toedsloth. Two-toed sloth species are commonly preferred animals in zoos. Gender determination is inevitable for these animals in captivity tobe raised successfully and healthily. Molecular genetic techniques allow high efficiency in taxonomic evaluations and gender identification inspecies that do not display sexual dimorphism. The PCR assay described here may be helpful for a rapid genetic analysis that can be widelyused in gender determination for two-toed sloths.Publication Evaluation of candidate gene effects and environmental factors on reproductive performance of holstein cows(South African Journal Of Animal Sciences, 2019-01-01) Ardıçlı, Sena; ARDIÇLI, SENA; Şamlı, Hale; ŞAMLI, HALE; Dinçel, Deniz; DİNÇEL, DENİZ; Soyudal, B.; Balcı, F.; BALCI, ÖMER FARUK; Bursa Uludağ Üniversitesi/Veteriner Fakültesi; 0000-0003-2758-5945; 0000-0002-8015-9032; 0000-0003-2382-1330; O-3394-2019; AAH-6488-2021; AAH-6192-2021This study investigated the impact of nine polymorphisms located in the CSN2, CSN3, CSN1S1, CSN1S2, and parity on reproductive traits. The analysis was conducted on 165 Holstein-Friesian cows. Genotypes were identified using PCR-RFLP. The data of reproductive traits for four lactations were evaluated. Statistical analysis was carried out using least squares of the GLM procedures. Results indicated that CSN2 had significant effects on days before first insemination and first insemination to pregnancy interval. The SNP at the CSN3 was significantly associated with gestation length. A novel effect of OLR1-C223A on age at first calving was observed in the present study. Moreover, DGAT1 and LGB markers were significantly associated with calving interval and days before first insemination, respectively. In addition, significant environmental effects were as follows: calving year with days before first oestrus, days open, and first insemination to pregnancy interval; season with days open, first insemination to pregnancy interval, and calving interval; parity with days before first oestrus and days open. The present results and novel associations may therefore be useful and indicative for future studies on a genetic basis of cattle reproduction traits.Publication Association of the caprine calpastatin Msp polymorphism with growth and reproduction traits in Saanen goats(Hellenic Veterinary Medical Soc, 2021-07-01) Ardıçlı, Sena; Üstüner, Hakan; Arslan, Oğuzhan; ARDIÇLI, SENA; ÜSTÜNER, HAKAN; ARSLAN, OĞUZHAN; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Zootekni Anabilim Dalı; 0000-0003-2758-5945; 0000-0002-1439-8502; O-3394-2019; GRY-9670-2022; JHO-8192-2023This study was designed to evaluate the effects of calpastatin (CAST) Mspl polymorphism on some growth and reproduction traits, including birth weight, first breeding weight, litter size, and average daily weight gain in Saanen goats. In this sense, blood samples obtained from 73 purebred female Saanen goats were used for genotyping. Genomic DNA was isolated by the phenol-chloroform method and used to determine CAST genotypes, including MM, MN, and NN, by means of the PCR-RFLP method. The population genetic parameters were estimated based on allelic distribution and the data were statistically analysed using analysis of variance (ANOVA) using a general linear model (GLM). Results revealed that N allele frequency was remarkably high (0.64) and the MM genotype was not present. The frequency of the heterozygous genotype was 59.62%. Concerning ANOVA results, significant differences were found between genotypes of the CAST locus concerning birth weight (P<0.05). In this respect, animals with the NN genotype were associated with higher birth weight means (2.85 +/- 0.29 kg) compared to heterozygous animals (2.53 +/- 0.24 kg). There was no significant association between the CAST marker and any of the remaining phenotypic traits evaluated. The present results suggest that focusing on this genomic region may be particularly useful in improving birth weight in goats which can be considered as an early indicator of post-weaning animal growth and survival.Publication Determination of the alterations in quality parameters and consumer preference of dry-aged beef based on different periods of ageing using a purposive incubator(Hellenic Veterinary Medical Soc, 2021-01-01) Üstüner, Hatice; Ardıçlı, Sena; Arslan, O.; Üstüner, Hatice; ARDIÇLI, SENA; Arslan, O.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Zootekni Anabilim Dalı; O-3394-2019; GRY-9670-2022; O-3394-2019; JHO-8192-2023The aim of this study was to evaluate beef quality traits including pH, water holding capacity, cooking loss, meat colour, and Warner-Bratzler shear force in dry-aged beef and to determine the most favorable ageing time based on beef quality parameters and consumer acceptability. In this respect, longissimus dorsi samples were obtained from Simmental bulls and stored for an ageing period up to 21-days, and thus, the beef quality evaluation was performed at 0-day, 7-day, 14-day, and 21-day of the experimental period. Results revealed that the lowest shear force value was observed in beef samples on day-14 whereas the lowest pH value was determined in samples on day 21. Moreover, water holding capacity and beef colour values were significantly differentiated based on dry-ageing (P<0.05). In sensory panel evaluation, a significant difference is found only in meat colour rating (P<0.05). There was no significant difference between ageing periods and cooking loss. The most important technical point is that increasing dry-ageing time from 14 to 21 days did not desirably affect quality traits and sensory scores. Hence, dry-ageing for 14 days seemed to be the most economically efficient application. Taken altogether, the present results suggest that the potential for use of dry-ageing should be considered as an alternative method to produce high-quality beef with respect to the optimum ageing process.Publication Comparison of cytological, microbiological and histopathological findings of genital tracts in cows with different degree perineal conformation disorder(Sivar-soc Italiana Veterinari Animali Reddito, 2021-02-01) Intas, Kamil Seyrek; Salci, Emsal Sinem Ozdemir; ÖZDEMİR SALCI, EMSAL SİNEM; Yavas, Ozkan; YAVAŞ, ÖZKAN; Ardicli, Ozge Yilmaz; Sonmez, Gursel; SÖNMEZ, GÜRSEL; Demirbilek, Serpil Kahya; KAHYA DEMİRBİLEK, SERPİL; Ardicli, Sena; ARDIÇLI, SENA; Bursa Uludağ Üniversitesi/Veteriner Fakültesi.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı.; 0000-0001-6077-0478; 0000-0003-2758-5945; KHD-4075-2024; O-3394-2019; AAG-7421-2021; AAH-5294-2021The aim of this study is to investigate cytological, microbiological and histopathological findings of the genital tracts in cows with different degree perineal conformation (PC) disorder. Totally, 28 cows brought the slaughterhouse were materials of the study. Information about to age, body weight, reproductive status, number of parturitions, days in milk (DIM) and the reason for slaughtering of the cows was obtained in the anamnesis learned from the owners. Before slaughtering, cows were evaluated in terms of PC and then they were divided into four groups with equal numbers of cows according to PC disorders: group I (GRI) normal; group II (GRII) mild; group III (GRIII) moderate and group IV (GRIV) severe. PC disorders of the cows were graded on a scale in terms of vulvar angle, vulvar length over ischial arch, depth of anus and perineal length. A body condition score (BCS) was also evaluated in the cows. The perineal region and vagina were inspected for vaginal discharge and vaginal mucosal appearance, respectively. Pneumovagina was classified as negative, suspicious and positive. Sterile swab samples were taken from vagina, cervix and uterus for bacteriological culture and identification. For cytological examination, smears were taken from vagina, cervix and uterus. Tissue samples were taken from vagina, cervix and uterus for histopathological examination. Statistically, Pearson's correlation, Fisher's exact tests and regression analysis were performed for all data. Clinically, characters of the vaginal discharge (serous, foamy, mucous, purulent and urine-mixed) and vaginal hyperemia increased in GRIII and GRIV. Microbiologically, as parallel to the PC disorder, E. coli in Enterobacteriaceae family was the most common bacterium in Group III and Group IV. As PC disorder in the groups increased, cytological examination findings were found to be significant, similar to clinical and microbiological examination. However, histopathological examination gave more meaningful results in groups. Statistic results pointed out that difference between the groups in terms of vaginal mucosa color, cervical and uterine microbiology was significant. In conclusion, PC disorder in cows constitutes predisposition for many genital canal diseases that may be the cause of infertility. The presence of genital canal diseases can be suspected in cattle using the PC scale. Therefore, it is appropriate to evaluate the health status of the genital canal with other diagnostic methods (microbiology, cytology and histopathology), especially in cows with PC disorder.Publication Determination the effect of CSN1S1, CSN3 and AGPAT6 genes and lactation rank on physicochemical properties of goat milk(Sivar-soc Italiana Veterinari Animali Reddito, 2020-08-01) Deniz, Dincel; DİNÇEL, DENİZ; Sena, Ardicli; ARDIÇLI, SENA; Hale, Samli; ŞAMLI, HALE; Faruk, Balci; BALCI, ÖMER FARUK; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı.; 0000-0002-8015-9032; 0000-0003-2758-5945; AAH-6192-2021; O-3394-2019The physiochemical properties of milk are important factors in terms of the process of dairy production, quality, and profitability. However, the knowledge about the effect of genetic factors such as CSN1S1, CSN3, and AGPAT6 genes on these traits is insufficient. The objective of this study was to determine the effect of these genes and lactation rank (LR) on total acidity, citric acid, density (density), free fatty acid (FFA), freezing point degree (FPD) and urea parameters. A total of fifty (n=50) Saanen goats, which is known as the highest milk-producing breed within the goats were genotyped using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) methods. For genetic analysis, the blood samples were taken from the jugular vein by aseptic conditions. The DNA isolations were performed by the phenol-chloroform method from blood samples. Milk samples were collected during the lactation and evaluated by Fourier transform infrared (FTIR) spectroscopy for the physicochemical properties of Saanen goat milk. The statistical analysis was carried out using the least-squares of the GLM procedures. The results indicated that no significant differences were confirmed between the investigated genes with the physicochemical properties of goat milk. However, the effect of the AGPAT6 gene on density and the effect of LR on citric acid composition was found tended to be significant. Moreover, we determined the significant correlations between the physicochemical properties in the present study. According to the results, acidity was correlated with the citric acid (-0.304), density (0.641), FFA (-0.332) significantly. Other significant correlations were observed between the citric acid and FPD (0.275), LR (-0.313); the density and FFA (-0.315), FPD (0.436); the urea and LR (-0.369). These results and novel correlations may be useful for future studies on evaluating the potential impact of AGPAT6 gene on these traits to achieve breeding and commercial goals in the goat production and dairy industry.Publication Fattening performance and carcass traits of imported simmental bulls at different initial fattening age(Sivar-soc Italiana Veterinari Animali Reddito, 2020-08-01) Ustuner, Hakan; ÜSTÜNER, HAKAN; Ardicli, Sena; ARDIÇLI, SENA; Arslan, Oznur; Brav, Fahir Cankat; Bursa Uludağ Üniversitesi/Veteriner Fakültesi; 0000-0003-2758-5945; O-3394-2019; AAG-9127-2021Fattening performance is one of the most important traits in cattle breeding. These traits are closely associated with adequate initial weights and slaughter endpoints. In this context, the aim of this study was to investigate optimum initial fattening age of imported Simmental young bulls and to provide a detailed analysis of initial fattening age effects on fattening performance and carcass characteristics. Seventy five animals were purchased from a single commercial farm where they had been reared under identical production conditions and were allocated into four groups according to age and live-weight as follows: Group I (4 months of age), Group II (6 months of age), Group III (8 months of age), and Group IV (10 months of age). All animals were housed in semi-open pens and were fed ad libitum with the same appropriate diet for 12 months. The animals were slaughtered in a commercial abattoir according to standard routines and carcass traits including hot and chilled carcass weights, dressing percentage, and chiling loss were determined. Morever, performance traits including total weight gain, feed conversion rate, average daily weight gain, and dry matter intake were estimated. Results revealed that the initial fattening age of bulls showed significant effects on total weight gain and chilled carcass dressing. In this context, Group I was characterized by the highest total weight gain, whereas, Group IV had the highest values for chilled carcass dressing. During early fattening periods, first three periods, average daily weight gain was significantly different among the treatments. This study pointed out that, concerning importation, younger Simmentals with the initial fattening age of four months may be more suitable and profitable for medium-term fattening. The present results may have a potential to influence adequate management practices in Simmental farms, and moreover, the recent strategies for importation of cattle.