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BÜYÜKCANGAZ, ESRA

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BÜYÜKCANGAZ

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    Publication
    A 13-Plex PCR for high-resolution melting-based screening of clinically important Staphylococcus species in animals
    (Elsevier, 2019-09-01) Ata, Zafer; Büyükcangaz, Esra; BÜYÜKCANGAZ, ESRA; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.; AAL-2323-2020
    A single-tube multiplex real-time PCR targeting the nuclease (nuc) gene and subsequent high-resolution melting analysis (HRMA) were used to identify 13 different Staphylococcus species. The nuc gene was targeted due to its low intraspecies variation and the greater interspecies variation than the 16S rRNA gene in Staphylococcus. We used HRMA software that can store and compare HRMA profiles from different runs as long as the runs contain the same reference reaction. Thus, we reduced the 14 PCRs to 2 different PCRs, one targeting the unknown sequence and the other targeting the reference sequences to screen 13 different Staphylococcus species. The specificity of the developed method was tested on 16 different Staphylococcus reference strains and 115 different field strains that were isolated from the milk of cattle with subclinical mastitis. We conclude that the method can be used to quickly and cost-effectively differentiate Staphylococcus aureus (S. aureus) from other Staphylococcus species (S. epidermidis, S. lugdunensis, S. schleiferi, S. hyicus, S. chromogenes, S. lentus, S. haemolyticus, S. xylosus, S. saprophyticus, S. warneri, S. simulans and S. hominis).
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    Postmortem findings on a group of Pica pica (Passeriformes: Corvidae)
    (Ankara Üniversitesi, 2019-01-01) Girişgin, Ahmet Onur; Alasonyalılar Demirer, Aylin; Büyükcangaz, Esra; Khider, Mohammed; Birlik, Sezen; İpek, Volkan; GİRİŞGİN, AHMET ONUR; ALASONYALILAR DEMİRER, AYLİN; BÜYÜKCANGAZ, ESRA; Khider, Mohammed; BİRLİK, SEZEN; İpek, Volkan; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Parazitoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.; 0000-0002-0020-2708; 0000-0001-5874-7797; AAL-2323-2020; B-5286-2017; ABZ-7197-2022; AAB-9963-2020; EKS-2415-2022; DAZ-7569-2022
    Common magpies (Corvidae: Pica pica) distribute through rural and urban areas of Turkey. Because of their distribution in urbanised regions, magpies may have some potential infectious agents which may relate to domestic animals and humans. In this study, eight common magpies brought to the animal hospital in need of medical intervention were examined for endoparasites and bacteria in a one-year period. Additionally, histopathologic examinations with related organs were carried out along with endo-parasitical, cytological and microbial examination the following necropsy. As results of the necropsies, three parasite species including two helminths and one protozoan (Passerilepis sp., Brachylaima sp., Isospora rochalimai, respectively) were identified, while Staphylococcus xylosus, S. sciuri, Escherichia coli, Klebsiella pneumonia, Salmonella spp. were isolated after microbiological examination. Histopathology revealed that subacute focal mycotic pneumonia, chronic nonpurulent granulomatous gastroenteritis, verminous enteritis, and the presence of paratyphoid nodules in liver. Both of the parasites and bacteria are the first records for Turkey's helminth/bacterial fauna in wild birds.