Person: NAK, DENİZ
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NAK
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DENİZ
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Publication Evaluation of serum anti-Mullerian hormone concentrations following treatment with vitamin D in Holstein Friesian heifers(Wiley, 2023-10-03) Koca, Davut; Nak, Yavuz; Şendağ, Sait; Nak, Deniz; Avcılar, Talha; Şahin, Mustafa Eren; Arslan, Talha; Özyiğit, Musa Özgür; Wehrend, Axel; NAK, YAVUZ; NAK, DENİZ; Avcılar, Talha; Şahin, Mustafa Eren; ÖZYİĞİT, MUSA ÖZGÜR; Veteriner Fakültesi; Patoloji Ana Bilim Dalı; IZN-9713-2023; LRU-4161-2024; EMG-5035-2022; IOB-6085-2023; JJX-7351-2023Anti-Mullerian hormone (AMH) levels in the blood of dairy cattle are associated with the count of antral follicles (AFC), ovarian function. Numerous studies, particularly in human medicine, have shown that vitamin D3 (Vit D3) has a positive effect on AMH levels. To our knowledge, the effects of Vit D3 on serum AMH levels in heifers have not been investigated. The goal of the current study was to evaluate the concentrations of serum AMH in dairy heifers following Vit D3 treatment. The study included 20 healthy non-pregnant Holstein Friesian heifers. These animals came to estrus at least once. All heifers received a single intramuscular dosage of 5 million IU Vit D3 (Cholecalciferol). Blood samples were collected from the coccygeal veins of all animals before and after Vit D3 injection (days 7, 14, and 28) for the measurement of AMH and 25(OH)D concentrations. In our analysis, we observed that after exogenous Vit D3 administration, circular 25(OH)D increased constantly (up to day 28 after Vit D3 injection) in all animals. On day 28, AMH concentrations saw a 10% increase in comparison with those measured the day before the Vit D3 injection. However, a comparison of AMH concentrations measured across days did not reveal statistically significant differences between Day 0, 7, 14, and 28 levels (p = .10). Furthermore, no statistical correlation was observed between the pairs (age-AMH) and (weight-AMH). Considering all times, no correlation was found between 25(OH)D and blood AMH levels. These findings demonstrated that exogenous Vit D3 did not affect serum AMH in Holstein Friesian heifers.Publication Evaluation of ovsynch and cidr plus ovsynch protocols to improve reproductive efficiency in lactating dairy cows(Zoological Soc Pakistan, 2019-10-01) Shahzad, Abid Hussain; Sattar, Abdul; Ahmad, Nasim; Ahmad, Ijaz; Nak, Yavuz; NAK, YAVUZ; Nak, Deniz; NAK, DENİZ; Tıp Fakültesi; Kadın Hastalıkları ve Doğum Ana Bilim Dalı; 0000-0002-8623-8292; 0000-0002-8153-0075; 0000-0002-4280-6673; AAH-5494-2021Appropriate postpartum reproductive management plays a vital role in dairy farm economics. Primary objective of the present study was to compare the efficiency of standard Ovsych protocol (OVP0) and its modified forms (OVP5 and OVP7) as postpartum reproductive management tools in cyclic dairy cows. In total, 167 Holstein cows were randomly divided into three treatment groups. The OVP0 group was comprised of 58 cows. Other two groups, OVP5 (n=55) and OVP7 (n=54), were similar to OVP0 except the intravaginal insertion of controlled internal drug release (CIDR) inserts for 5 or 7 days, respectively. Pregnancy was diagnosed using ultrasonography on d30, d60 and d90 post AI. Ovulatory follicle diameter was measured at timed AI and progesterone profile (ng/mL) on d30 and d60 post AI. Pregnancy rate was analyzed by Chi-square procedure while ovulatory follicle diameter and Progesterone profile by one way ANOVA (alpha=0.05). Ovulatory follicle diameter (Mean +/- SEM) was 15.19 +/- 0.17 (OVP0), 15.30 +/- 0.21 (OVP5) and 15.24 +/- 0.19 (OVP7), respectively. The P4 concentration has significant (P<0.05) difference among OVP0 (6.52 +/- 0.32), OVP5 (7.75 +/- 0.38) and OVP7 (7.58 +/- 0.26) on d30 post AI. This difference was non-significant (P > 0.05) on d60 post AI in OVP0 (6.37 +/- 0.49), OVP5 (6.75 +/- 0.36) and OVP7 (6.80 +/- 0.41), respectively. On d30 post AI, pregnancy rate was 39.70, 42.60 and 45.50% in OVP0, OVP5 and OVP7 groups, respectively (P=0.48). Corresponding pregnancy rate on d60 (P=0.39) and d90 (P=0.61) was 36.20, 32.80% in OVP0, 43.80, 41.80% in OVP5 and 37% in OVP7 group. Overall pregnancy loss was 17 (OVP0), 08 (OVP5) and 13% (OVP7), respectively (P=0.62). In conclusion, although pregnancy rate has non-significant difference among all three breeding protocols but numerically, improved pregnancy rate and reduced pregnancy loss was observed in OVP5 group.Publication Effect of butylated hydroxytoluene on post-thawed semen quality of beetal goat buck, capra hircus(Zoological Soc Pakistan, 2015-02-01) Iqbal, Z.; Ijaz, A.; Aleem, M.; Shahzad, A. H.; Sohail, M. U.; Nak, Deniz; Nak, Yavuz; Abbas, S.; NAK, DENİZ; NAK, YAVUZ; Tıp Fakültesi; Doğum ve Jinekoloji Bölümü; AAH-5494-2021; FPJ-3706-2022Present study was conducted to evaluate the effect butylated hydroxytoluene (BHT) on post-thawed semen quality of Beetal goat buck semen. Semen was obtained from six bucks using artificial vagina and cryopreserved in tris egg yolk extender and semen quality was assessed on the basis of post-thaw sperm motility, viability, plasma membrane integrity, and acrosomal membrane status. Ejaculates were pooled and extended to the concentration of 2 x 10(9) spermatozoa per mL in tris egg yolk extender (300 mOsmol/L). Tris egg yolk extender containing various concentrations of BHT (0.0, 2.0 and 5.0 mM) was prepared. French straws (0.5 mL) were manually filled with semen, gradually cooled from 39 degrees C to 4 degrees C and finally, cryopreserved in liquid nitrogen at -196 degrees C. Five straws from each treatment [BHT (0.0, 2.0 and 5.0 mM)] were thawed and evaluated under phase-contrast microscope (40 x) for sperm motility, whereas sperm viability, plasma membrane integrity and acrosomal integrity were assessed by the supravital staining, hypo-osmotic swelling test (HOST) and normal acrosomal reaction, respectively. The results showed that only acrosomal integrity was improved (P < 0.05) by the addition of BHT in semen extender. Motility was suppressed (P < 0.05) by increasing BHT concentration. The maximum motility of sperm was achieved with 0.0 mM BHT. The HOST response and viability of spermatozoa were increased by addition of 2 and 5 mM BHT, but this increment was not statistically significant. In conclusion, the addition of BHT to semen extender can partially improve semen quality of Beetal goat.Publication Sry-negative xx sex reversal in an english cocker spaniel: A case report(Czech Academy Agricultural Sciences, 2015-01-01) Shahzad, A. H.; Nak, D.; NAK, DENİZ; Gülten, T.; Karkucak, M.; Yılmaz, R.; Nak, Y.; NAK, YAVUZ; Şimşek, G.; Veterinerlik Fakültesi; 0000-0002-0944-2230; 0000-0002-8623-8292; ABG-8940-2020; AAH-5494-2021A SRY-negative XX male describes a female karyotype with testicular tissue resulting from abnormal gonadal development. In such cases male gonad formation takes place in the absence of a Y chromosome which supports the hypothesis that some other genes are also responsible for testicular development. The present case report describes a one-year-old dog brought to the hospital with a complaint of no oestrus cycle. Apparently normal masculine bitch had an enlarged clitoris with an os on radiograph. A gonado-hysterectomy was performed. Instead of ovarian structures both testicular masses were present and a normal complete bicornuate uterus with a normal cervix was observed. The histology of both gonads showed two testes, each with its ductus deferens and the uterus with typical histological structure. Cytogenetic evaluation of peripheral blood lymphocytes revealed a female with a normal karyotype (78, XX). Polymerase chain reaction (PCR) analysis of genomic DNA showed that the SRY gene was absent.Publication Evaluation of some systemic inflammatory biomarkers in canine malignant mammary tumors [i](Kafkas Univ, Veteriner Fakultesi Dergisi, 2023-09-01) Koca, Davut; Ekici, Zeynep Merve; Özyiğit, Musa Özgür; ÖZYİĞİT, MUSA ÖZGÜR; Nak, Deniz; NAK, DENİZ; Avcı Küpeli, Zehra; Kuruoğlu, Fikriye Ecem; Avcılar, Talha; Gümüş, Oğulcan; Nak, Yavuz; NAK, YAVUZ; Tıp Fakültesi; 0000-0002-6715-8529; 0000-0002-7962-6959; 0000-0001-7034-9644The aim of this study is to investigate whether neutrophil-to-lymphocyte ratio (NLR), lymphocyte-to-monocyte ratio (LMR), platelet-to-lymphocyte ratio (PLR), systemic immune-inflammation index (SII), albumin-to-globulin ratio (AGR), and prognostic nutritional index (PNI) parameters could be used as biomarkers for canine malignant mammary tumors (MMTs), and the changes in these parameters according to different tumor (T), lymph node (N), and metastasis (M) stages (TNM I-II-III, TNM IV, TNM V) and the number of affected mammary glands (single, multiple). Thirty-seven with MMT and 20 healthy dogs were used in this study. Complete blood count and biochemistry analysis were performed in all dogs. Tumor material is removed by tru-cut and sent to the pathology laboratory for diagnosis. NLR, PLR, and SII values increased, and LMR and PNI values decreased in dogs with MMT. Median NLR values increased and median LMR and PNI values decreased as the TNM stage progressed. In dogs with a single MMT, median NLR, and PLR values were found to be lower than in dogs with multiple MMTs, and median LMR, SII, AGR, and PNI values were higher. The present results indicated that NLR, LMR, PLR, SII, and PNI parameters could be used as biomarkers for canine MMT. Also, NLR, LMR, PLR, SII, PNI, and AGR parameters may be valuable biomarkers that reveal the degree of systemic immune response according to different TNM stages and the number of affected mammary glands.Publication Ascorbic acid inclusion in semen extender improves the post-thawed semen quality of sahiwal cattle (Bos indicus)(Zoological Society of Pakistan, 2015-12-01) Sohail, M. U.; Shahzad, A. H.; Iqbal, S.; Shabbir, M. Z.; Iqbal, Z.; Abbas, S.; Younus, M.; Nak, D.; Nak, Y.; Arshaad, T.; NAK, DENİZ; NAK, YAVUZ; FPJ-3706-2022; AAH-5494-2021The antioxidant effects of ascorbic acid at different inclusion rates were evaluated upon semen (n=150) from mature Sahiwal cattle bulls (Bos indicus, n = 6) in summer season. The semen was diluted in Tris-citrate egg yolk extender containing different levels (0.0, 1.0, 2.0, 3.0, and 4.0 mg/mL) of ascorbic acid at 37.0 degrees C. Semen was cryopreserved at -196 degrees C in 0.5 mL French straws. Semen straws were thawed at 37 degrees C to assess the spermatozoa indices in terms of motility, viability, plasma membrane and acrosomal integrity under phase-contrast microscope. Supravital staining, hypo-osmotic swelling test and normal acrosomal reaction analysis tests were also performed for viability, plasma membrane and acrosomal integrity, respectively. The data were subjected to one way ANOVA. The results revealed significant improvement (P< 0.05) in post thaw sperm quality in terms of motility, vitality, acrosomal and plasma membrane integrity by the increasing concentrations of ascorbic acid (1.0, 2.0, 3.0, 4.0 mg/mL) in semen extender. Quality parameters were higher (P< 0.05) when 3.0 mg/mL of ascorbic acid was added in semen extender followed by 2.0 and 4.0 mg/mL. In conclusion the addition of ascorbic acid at the rate of 3.0 mg/mL in the semen extender may improve the semen quality of Sahiwal cattle bull.Publication Evaluation of synchronization protocols and methods of early pregnancy diagnosis in dairy cattle(South African Journal of Animal Sciences, 2020-01-01) Shahzad, A. H.; Sattar, A.; Ahmad, N.; Ahmad, I; Yousaf, M. S.; Abbas, S.; Blouch, R. S.; Nak, D.; Nak, Y.; Ray, D. L.; Silvia, W. J.; NAK, YAVUZ; NAK, DENİZ; Veteriner Fakültesi; Jinekoloji Ana Bilim Dalı; LRU-4161-2024; DKG-3473-2022The studies aimed to evaluate the pregnancy rate (PR) for timed artificial insemination (TAI) after G7G-Ovsynch, modified G7G-Ovsynch (MG7G-Ovsynch) and Ovsynch protocols and to assess the accuracy of using pregnancy-associated glycoproteins (PAGs) and plasma progesterone (P-4) in pregnancy diagnosis compared with ultrasonography (US). In study 1, Holstein cows (n = 37) were bred by TAI following the G7G-Ovsynch protocol (n = 19) or MG7G-Ovsynch (n = 18). Pregnancy was evaluated by US at days 31, 59, and 87 after breeding. The PR was not different for the G7G-Ovsynch and MG7G-Ovsynch. Blood and milk samples were collected on day 3 after insemination and then weekly through day 59 post TAI in cows diagnosed as not pregnant on day 31 and through day 87 in pregnant cows. PAGs were measured using ELISA and P-4 by radioimmunoassay (RIA). In the second study, Holstein cows (n = 212) were bred by TAI following G7G-Ovsynch protocol (n = 110) or standard Ovsynch (n = 102). Cows were subjected to pregnancy diagnosis on days 30, 60, and 90. A subset (n = 15 in each group) was subjected to blood and milk samples on days 30, 45, 60, 75, and 90 to measure PAGs and P-4. In study 2, PR was not significantly different between synchronization protocols on days 30, 60, and 90. Pregnancy loss averaged 15% between day 30 and day 90. The use of PAGs and P-4 proved equally effective in diagnosis of pregnancy. Thus, G7G-Ovsynch was deemed the protocol of choice in postpartum cows, and PAGs assayed in milk or plasma could be used to diagnose pregnancy.Publication Evaluation of serum anti-Mullerian hormone concentrations following treatment with vitamin D in dairy heifers(Wiley, 2023-02-01) Koca, D.; Nak, Y.; Sendag, S.; Nak, D.; Arslan, T.; Wehrend, A.; NAK, DENİZ; NAK, YAVUZ; Veteriner Fakültesi; Jinekoloji Ansbilim Dalı; LRU-4161-2024; DKG-3473-2022Publication Efficacy and toxicity of doxorubicin and cyclophosphamide for the neoadjuvant treatment of locally advanced stage canine mammary tumors(Kafkas Univ, Veteriner Fakultesi Dergisi, 2020-11-01) Shahzad, Abid Hussain; Kuruoglu, Fikriye Ecem; Özyiğit, Musa Özgür; ÖZYİĞİT, MUSA ÖZGÜR; Nak, Deniz; NAK, DENİZ; Küpeli, Zehra Avcı; Ekici, Zeynep Merve; Koca, Davut; KOCA, DAVUT; Avcılar, Talha; Nak, Yavuz; NAK, YAVUZ; Sahin, Mustafa Eren; ; Veteriner Fakültesi; Doğum ve Jinekoloji Ana Bilim Dalı; 0000-0002-6715-8529; 0000-0003-1853-4679; 0000-0002-7962-6959; 0000-0001-7034-9644; 0000-0002-8623-8292; AAH-5494-2021; AAH-2873-2021; AAI-6709-2021; AAR-6478-2021The aim of this study was to investigate the effect and toxicity of a doxorubicin cyclophosphamide combination for neoadjuvant use in dogs with locally advanced mammary tumors. A total of 16 dogs with locally advanced mammary tumors aged 8-14 years was used in this study. Samples were taken from all the dogs via Tru-Cut biopsy and fine needle aspiration biopsy of the lymph nodes prior to neoadjuvant chemotherapy. The samples were sent to the pathology laboratory for histopathological examination. Based on the histopathology results, mammary tumors were diagnosed as adenocarcinoma (n=13), tubulopapillary carcinoma (n=2) and malignant mixed tumors (n=1). For the neoadjuvant chemotherapy, doxorubicin (25-30 mg/m(2)) and cyclophosphamide (100 mg/m(2)) were administered via slow IV injection 4 times at 3-week intervals. Clinical findings such as fever, vomiting, anorexia, lethargy, weight loss, alopecia, enteritis, hematuria, and skin ulceration and laboratory findings such as a decrease in neutropenia and hematocrit values were observed. Based on the longest diameter of the tumors before and after four weeks of chemotherapy, a complete response was detected in 2 dogs (12.5%), a partial response was observed in 9 dogs (56.25%) and the disease was stable in 5 dogs (31.25%). According to results, the toxicity, and efficacy of a doxorubicin and cyclophosphamide combination for neoadjuvant chemotherapy in dogs with locally advanced mammary tumors is acceptable, and the treatment is promising.