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MECİTOĞLU, GÜLNAZ

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MECİTOĞLU

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GÜLNAZ

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2015 - 201912020 - 20211
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    Publication
    Distributions of CYP19, ERα and PGR allele frequencies between fertile and subfertile Holstein-Friesian heifers
    (Kafkas Üniversitesi, 2015-11-01) Keskin, Abdulkadir; Öner, Yasemin; Yılmazbaş-Mecitoğlu, Gülnaz; Güner, Barış; Karakaya, Ebru; Elmacı, Cengiz; Gümen, Ahmet; KESKİN, ABDULKADİR; ÖNER, YASEMİN; MECİTOĞLU, GÜLNAZ; Güner, Barış; Karakaya, Ebru; ELMACI, CENGİZ; GÜMEN, AHMET; Uludağ Üniversitesi/Veteriner Fakültesi/Jinekoloji Anabilim Dalı.; Uludağ Üniversitesi/Ziraat Fakültesi/Hayvan Bilimleri Biyometri ve Genetik Bölümü.; 0000-0002-2904-8986; 0000-0003-2817-3221; 0000-0001-6414-6752; 0000-0003-4837-1858; 0000-0003-4819-0221; 0000-0002-3607-4365; V-7882-2019; AIF-3815-2022; ABB-3181-2020; AAH-3831-2021; AAH-1406-2021; AAG-4440-2019; AGY-3496-2022; ABG-2047-2020
    The aim of this study was to investigate the gene and genotype distributions of some mutations in the aromatase cytochrome P450 (CYP19), estrogen receptor alpha (ER alpha), and progesterone receptor (PGR) genes in fertile and subfertile Holstein-Friesian heifers using the PCR-RFLP method and comparing the distributions between groups. A total of 106 heifers were included the study, and the heifers that became pregnant after the first artificial insemination (n=51) were used as a fertile group. Heifers (n=55) with equal and more than 3 AIs were accepted as a subfertile heifers. Blood samples from all of the heifers were obtained for DNA isolation. While two alleles and three genotypes were found at the PGR and ER alpha loci, two alleles and two genotypes were detected at the CYP19 locus. The A allele and AA genotype, G allele and GG genotype, and C allele and CT genotype were found to be predominant in CYP19, ER alpha and PGR, respectively. According to the chi-square test (chi(2)), two of the groups investigated were in Hardy-Weinberg equilibrium for all gene loci. There were no differences detected in allele or genotype frequencies between the fertile and subfertile heifers.
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    Publication
    Effects of beta-carotene administration on fertility in lactating dairy cows
    (Agricultural Research Communication Centre, 2021-02-01) Bilen, Ebru Karakaya; ; Mecitoglu, Gulnaz Yilmazbas; MECİTOĞLU, GÜLNAZ; Bursa Uludağ Üniversitesi/Veterinerlik Fakültesi.; 0000-0003-2817-3221; AAG-4440-2019
    Background: Beta-(beta-) carotene, is the precursor to vitamin A, in particular, has some potential benefits on reproduction. The main objective of this study was to investigate the efficacy of beta-carotene administration on fertility following either prostaglandin F2 alpha (PGF) induced estrus or Ovsynch protocol in lactating dairy cows.Methods: Cows with at 47 +/- 3 postpartum days were divided into two groups: beta-carotene group (beta C, n=139) was treated with injectable beta-carotene while untreated cows served as control (CON, n=227). In both groups, PGF was administered and heatmount detectors were applied at 54 +/- 3 days postpartum. Cows detected in estrus after PGF were inseminated. Cows that had not been detected in estrus were divided into two groups 7 days after PGF administration; beta C-ovs (n=137) and CON-OVS (n=89). Ovsynch protocol was initiated 4 days after beta-carotene administration.Result: The estrus detection rate was similar between the beta C and CON groups (P = 0.19). Pregnancy per AI (P/AI) on d 31 was also similar between groups (P = 0.93). In the Ovsynch protocol, ovulation to the first GnRH and ovulatory follicle diameter at the time of insemination did not differ between groups. No difference was observed in P/AI at d 31 (P = 0.13). The results of this study indicated that beta-carotene administration had no effect on fertility either PGF induced estrus or Ovsynch protocol in dairy cows.