Person: ÜLGEN, MİHRİBAN
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ÜLGEN
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MİHRİBAN
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Publication Investigation of contagious agalactia by bacteriological and PCR methods in sheep and goats(Kafkas Üniversitesi, 2015-01-01) Göçmen, Hüban; Ülgen, Mihriban; Çarlı, K. Tayfun; Onat, Kaan; Kahya, Serpil; Özdemir, Ümit; Mat, Burak; Göçmen, Hüban; ÜLGEN, MİHRİBAN; CARLI, KAMİL TAYFUN; KAHYA DEMİRBİLEK, SERPİL; Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü/Veteriner-Mikrobiyoloji Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakultesi/Mikrobiyoloji Anabilim Dalı.; 0000-0002-2245-5781; 0000-0002-6307-5179; AAG-8117-2021; B-9095-2018; AAH-2842-2021; E-3867-2010The aim of this study was diagnosis that occurrence of Contagious Agalactia by bacteriological and molecular methods in sheep and goats. A total of 339 samples from sheep and goats in Bursa, Balikesir, Canakkale and Edirne provinces were examined by bacteriological and molecular methods. The samples were 162 milk samples, 147 eye swabs, 15 joint fluids, 11 nasal swabs and 4 lung tissue. In bacteriological examination, 29 isolates were evaluated as Mycoplasma sp.. As a result of biochemical tests and growth inhibition tests, 29 (8.55%) Mycoplasma sp. were identified as 25 (7.37%) Mycoplasma agalactiae, 2 (0.58%) Mycoplasma ovipneumoniae and 2 (0.58%) Mycoplasma arginini. In molecular diagnosis, polC gene-PCR results could be detected M. agalactiae positive with 9.14% rate. As a result of this, 5 milk samples and 1 lung tissue sample were detected positive by polC-PCR while negative by bacteriological examination. The results of polC-PCR detected M. agalactiae positive with 14.19% rate of milk samples, 13.33% rate of joint fluids, 2.72% rate of eye swabs and 50% rate of lung tissue samples but nasal swabs were detected as negative. In this study, presence of Contagious Agalactia were investigated by bacteriological and molecular methods and M. agalactiae was detected as a main agent which cause disease however other Mycoplasma species which cause disease were not observed.Publication The investigation by PCR and culture methods of foulbrood diseases in honey bees in south marmara region(Kafkas Üniversitesi, 2015-01-01) Borum, A. Ebru; Özakin, Cüneyt; Güneş, Ertan; Aydın, Levent; Ülgen, Mihriban; Çakmak, İbrahim; ÖZAKIN, CÜNEYT; Güneş, Ertan; AYDIN, LEVENT; ÜLGEN, MİHRİBAN; ÇAKMAK, İBRAHİM; Uludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji ve Enfeksiyon Hastalıkları Anabilim Dalı.; Uludağ Üniversitesi/Teknik Bilimler Meslek Yüksekokulu.; Uludağ Üniversitesi/Veteriner Fakültesi/Parazitoloji Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.; Uludağ Üniversitesi/Mustafakemalpaja Meslek Yüksekokulu.; 0000-0002-9347-8307; 0000-0001-5428-3630; 0000-0002-6307-5179; AAG-8392-2021; AAG-8117-2021; AAH-2558-2021; AAK-4470-2021; HLG-4450-2023American Foulbrood and European Foulbrood diseases of honeybees were examined in 725 beehives from 23 apiaries located in the South Marmara Region of Turkey. We determined that 19 apiaries were infected and the suspected clinical signs of foulbrood diseases were investigated in 102 beehives by PCR and cultural method. Broods and combs from colonies with suspected clinical symptoms of foulbrood diseases were collected and cultured for bacteriological examination. All of the specimens contaminated with bacteriae and 37 species of bacteriae were isolated such as Stapylococcus epidermidis, Bacillus subtilis, Corynebacterium jeikum, Corynebacterium pseudotuberculosis, Bacillus spp. All of these bacteria are related to human, animal and environmental origins. In this study, Paenibacillus larvae by PCR amplifying the 973-bp region PL1 and PL2 with 1f, Melissococcus plutonius amplifying the 973-bp region EFB-F and EFB-R gene were amplified. American Foulbrood causative agent Paenibacillus larvae and European Foulbrood causative agent Melissococcus plutonius were not detected in any sample examined by PCR and cultural methods. On the other hand, Paenibacillus alvei that is a seconder agent to European Foulbrood was found in two samples by cultural methods. In conclusion, the results showed that P. larvae and M. plutonius are not present in South Marmara Region. In this study, human, animal and environment originated agents were isolated.Publication Bacteriological and cytological findings of bronchoalveolar lavage fluids in foals with lower respiratory tract diseases(Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 2015-05-01) Kasap, Sevim; Göcmen, Huban; Çatık, Serkan; Onat, Kaan; Ülgen, Mihriban; Çetin, Cengiz; Kennerman, Engin; KASAP, SEVİM; Çatık, Serkan; Onat, Kaan; ÜLGEN, MİHRİBAN; Çetin, Cengiz; KENNERMAN, ENGİN; Uludağ Üniversitesi/Veteriner Fakültesi/Dahili Tıp Bölümü; Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobioloji Bölümü; 0000-0002-2245-5781; 0000-0002-3537-354X; AAG-8117-2021; AAP-7998-2020; J-8672-2018; JLJ-9087-2023; FQU-3039-2022; J-8672-2018; FDU-5862-2022Lower respiratory tract disease is one of the most common causes of economic loss in foals. There are several methods used for the diagnosis of this disease. Bronchoalveolar lavage is one of the most important technique for bacteriological and cytological examination. The aim of this study was to evaluate BAL samples' cytologic and bacteriologic examination of foals with lower respiratory tract disease. In the comparison of cytologic examination of BAL samples with bacterial agents and without bacterial agents statistical differences in the percentage of neutophils, macrophages (P<0.001) and lymphocytes (P<0.05) were defined. Also according to the bacteriologic examination results of the BAL samples in 20 foals following agents were mainly detected: Streptococcus equi subsp. zooepidemicus (10), Staphylococcus aereus (7), Escherichia coli (3). Totally 25 aerobic/facultative gram (+) and gram (-) bacils were isolated. In conclusion, the BAL technic is useful for cytological and bacteriological examination in horses with lower respiratory tract diseases.Publication Clinical, microbiological and pathological findings in the genital tract of infertile mares(Indian Veterinary Journal, 2003-08-01) Sönmez, Gürsel; Seyrek-İntaş, K; Ülgen, Mihriban; SÖNMEZ, GÜRSEL; Seyrek-İntaş, K; ÜLGEN, MİHRİBAN; Uludağ Üniversitesi/Veteriner Fakültesi/Pataholoji Bölümü; Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobioloji Bölümü; 0000-0002-2524-3274; AAG-8117-2021; AAP-7233-2020; AAH-7292-2019