Person: İPEK, AYDIN
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İPEK
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AYDIN
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Publication Targeting the epithelial-mesenchymal transition (emt) pathway with combination of wnt inhibitor and chalcone complexes in lung cancer cells(Wiley, 2023-07-14) Coşkun, Demet; Arı, Ferda; ARI, FERDA; Ertürk, Elif; ERTÜRK, ELİF; Onur, Ömer E.; Akgün, Oğuzhan; Aydın, İpek; İPEK, AYDIN; Bursa Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Biyoloji Bölümü.; Bursa Uludağ Üniversitesi/Tıbbi Labaratuvar Teknikleri .; 0000-0002-8410-1786; 0000-0002-6729-7908; A-5608-2019; JQI-3400-2023; AAG-7012-2021; IUO-8513-2023Non-small cell lung cancer (NSCLC) is the most common type of the lung cancer. Despite development in treatment options in NSCLC, the overall survival ratios is still poor due to epithelial and mesenchymal transition (EMT) feature and associated metastasis event. Thereby there is a need to develop strategy to increase antitumor response against the NSCLC cells by targeting EMT pathway with combination drugs. Niclosamide and chalcone complexes are both affect cancer cell signaling pathways and therefore inhibit the EMT pathway. In this study, it was aimed to increase antitumor response and suppress EMT pathway in NSCLC cells by combining niclosamide and chalcone complexes. SRB cell viability assay was performed to investigate the anticancer activity of drugs. The drugs were tested on both NSCLC cells (A549 and H1299) and normal lung bronchial cells (BEAS-2B). Then the two drugs were combined and their effects on cancer cells were evaluated. Fluorescence imaging and enzyme-linked immunosorbent assay were performed on treated cells to observe the cell death manner. Wound healing assay, real-time quantitative polymerase chain reaction, and western blot analysis were performed to measure EMT pathway activity. Our results showed that niclosamide and chalcone complexes combination kill cancer cells more than normal lung bronchial cells. Compared to single drug administration, the combination of both drugs killed NSCLC cells more effectively by increasing apoptotic activity. In addition, the combination of niclosamide and chalcone complexes decreased multidrug resistance and EMT activity by lowering their gene expressions and protein levels. These results showed that niclosamide and chalcone complexes combination could be a new drug combination for the treatment of NSCLC.Publication Changes in ph of digestive tract and cecal microflora composition in broilers fed with probiotic and prebiotic supplementation (SynerAll).(Oxford Univ, 2016-10-01) İpek, Aydın; Sözcü, Arda; İPEK, AYDIN; SÖZCÜ, ARDA; Uludağ Üniversitesi/Ziraat Fakültesi/Zootekni Bölümü; FCM-0798-2022; HXQ-7280-2023Publication The effects of different eggshell temperatures between embryonic day 10 and 18 on broiler performance and susceptibility to ascites(Facta-fundacio Arnco Ciencia Tecnologia Avicolas, 2015-07-01) İpek, Aydın; İPEK, AYDIN; Şahan, U.; ŞAHAN, ÜMRAN; Sözcü, A.; SÖZCÜ, ARDA; Bursa Uludağ Üniversitesi/Ziraat Fakültesi.; AAH-2966-2021; AAH-3973-2021; AAA-5866-2020; AAH-2551-2021The aim of the current study was to determine the effects of low (33.3-36.7 degrees C), control (37.8-38.2 degrees C) and high (38.9-40.0 degrees C) eggshell temperatures (ESTs) between days 10-18 of incubation on broiler performance and susceptibility to ascites. The effects of different ESTs on hatchling weight were significant. The highest residual yolk sac weight (7.7 g) and relative yolk sac weight (18.7%) were observed in high EST group, whereas the highest yolk-free body weight (36.22 g) was obtained in the control group. Relative post-hatch heart weight was lower in the high EST group (0.64%) compared with the control (0.84%) and low (0.88%) EST groups. At 6 weeks of age, body weights of broilers of the low, control, and high EST groups were 2172.6, 2543.9, 2282.6 g respectively. During the 6-week life span, the feed conversion ratio of broilers in the low (1.86) and high (1.83) EST groups was significantly worse than of those in the control EST group (1.68). Higher packed cell volume (PCV) and red blood cell (RBC) values were obtained in the high EST group relative to the other groups, whereas hemoglobin (Hb) level was lower in the control group than in the others. The relative heart weights and right ventricular to total ventricular (RV: TV) ratio for low, control, and high EST group were 0.46, 0.40, 0.37 g and 0.27, 0.28, 0.33% respectively. In conclusion, incubation length, yolk sac absorption, residual yolk sac weight, yolk-free BW, post-hatch performance, and heart, blood parameters, abdominal fluid accumulation at slaughter age are affected by changes in EST between incubation days 10 to 18.Publication The effects of high setter and hatcher temperatures during incubation on slaughter weight and carcass yield in broilers(Tübitak Bilimsel ve Teknolojik Araştırma Kurumu, 2015-01-01) İpek, Aydın; Sözcü, Arda; İPEK, AYDIN; SÖZCÜ, ARDA; Uludağ Üniversitesi/Ziraat Fakültesi/Zootekni Bölümü; AAH-2551-2021; AAA-5866-2020; AAH-3973-2021This study was carried out with the aim of determining the effects of higher setter and hatcher temperatures on slaughter weight and carcass yield. In experiment 1, setter temperatures were applied as control (37.8-38.2 degrees C) and high (38.9-40.0 degrees C) between days 10 and 18 during incubation. In experiment 2, hatcher temperatures were applied as control (36.8-37.0 degrees C) and high (38.8-39.0 degrees C) during the hatching period. A total of 240 chicks from each experiment were randomly selected after the hatching process was complete. At the end of the growing period, a total of 120 broilers from each experiment were weighed and slaughtered at 42 days of age. The carcass weight was lower in the higher temperature groups in both experiments. In the higher setter temperature group, the breast weight was lower with a value of 981.4 g, but the percentage of breast was higher with a value of 45.59%. In experiment 2, the weight and the percentage of breast was similar in the control and high hatcher temperature groups. In conclusion, slaughter weight and carcass yield are affected by higher setter and hatcher temperatures.Publication The effects of hatching time and feed access time on chick quality, organ development, blood parameters, and intestinal morphology in broilers(TÜBİTAK, 2020-05-26) Sözcü, Arda; İpek, Aydın; Kahraman, Mujdat Müfit; İpek, Volkan; İPEK, AYDIN; KAHRAMAN, MÜJDAT MÜFİT; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Zootekni Bölümü.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; AAH-2551-2021; CYB-9080-2022The current study was performed to investigate the effects of hatching time and feed access time on chick quality, organ development, blood parameters, and intestinal morphology in broilers. A total of 1935 hatching eggs were incubated at 37.8 degrees C with 55 to 60% RH conditions. Chicks were classified according to the hatching time (HT) as follows: chicks hatched before 482 h (early HT), between 482-496 h (medium HT) and 496-510 h (late HT). The hatched chicks were randomly divided into two groups to create two feed access (FA) time: early FA and late PA. Chicks with early PA started to consume feed at the end of each HT, whereas chicks with late FA started to feed at the end of the hatching period (at 510 h). Higher chick weight was observed in chicks in early HT and medium HT groups (P = 0.002). The chicks with late PA were heavier and taller than other chicks with FA. A higher Tona score (86.2) was observed for the chicks in medium HT (P = 0.001). Chicks in late HT group had a higher residual yolk weight, a lower yolk absorption, and a lower yolk free body mass. The chicks with late FA had a lower residual yolk weight, higher yolk absorption and, yolk free body mass (P < 0.01). Development of gizzard, heart, small intestine, and bursa of Fabricius were affected by both hatching time and feed access time. The lowest concentration of glucose was observed in chicks obtained from early HT with late FA (P = 0.002). Villus morphometric parameters in duodenum, jejunum, and ileum were affected by HT and FA time (P < 0.05). The current findings showed that the HT of chicks with regard to FA time markedly affected the development and growth pattern of chicks.Publication Comparison of performance, egg quality, and yolk fatty acid profile in two Turkish genotypes (Atak-s and Atabey) in a free-range system(Mdpi, 2021-05-15) Sözcü, Arda; İpek, Aydın; Oğuz, Züleyha; Gunnarsson, Stefan; Riber, Anja B.; SÖZCÜ, ARDA; İPEK, AYDIN; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Zootekni Bölümü.; 0000-0002-0955-4371; DUR-2892-2022 ; FCM-0798-2022Simple SummaryIn recent years, consumers have shown increased interest in healthy and safe food produced under improved animal welfare standards. Therefore, production systems proving outdoor access have gained popularity, increasing the need for knowledge on genotypes suitable for free-range systems. This study aimed to investigate the suitability of two Turkish layer genotypes, Atak-S (brown) and Atabey (white), in a free-range system. We evaluated laying performance, egg quality parameters, and yolk fatty acid profile. The egg production was higher in Atabey than Atak-S, whereas the eggs from Atak-S hens tended to be heavier and had a stronger shell structure than eggs from Atabey hens. Furthermore, eggs from Atabey hens had improved egg yolk and albumen content compared to eggs from Atak-S hens. The total saturated fatty acid content in yolk was higher in Atabey eggs than in Atak-S eggs at 56 weeks of age, whereas a higher yolk color score was observed in Atak-S eggs than in Atabey eggs. Our results could help free-range egg producers to improve production, as well as satisfy consumer demands regarding egg quality in organic production.Consumer interest in buying eggs from animal welfare-friendly systems with outdoor access is increasing, leading to an increase in the need for knowledge on genotypes suitable for free-range systems. Two Turkish laying hen genotypes, Atak-S (brown, n = 210) and Atabey (white, n = 210), were reared in a free-range system from 19-72 weeks of age, and their suitability for the system was assessed based on laying performance, egg quality, and yolk fatty acid profile. Mean hen-day and hen-housed egg production were found to be higher in Atabey than Atak-S (p < 0.01). The brown eggs from Atak-S hens tended to be heavier than the white eggs from Atabey hens (p < 0.01). Brown eggs obtained from Atak-S hens had a stronger shell structure (p < 0.01), while white eggs from Atabey hens had higher mean yolk index, albumen index, and Haugh unit than brown eggs (p < 0.05). At 56 weeks of age, total saturated fatty acid content in yolk was higher in white eggs than in brown eggs (p < 0.01). These findings related to genotype could help free-range egg producers in their choices for more profitable production and for meeting consumer demands on egg quality and egg yolk fatty acid levels.Publication Cytotoxic potential of rare plant salvia candidissima subsp candidissima on breast cancer cells(Inst Tecnologia Parana, 2023-01-01) Özel, Mustafa Zafer; Fırat, Mehmet; Ertürk, Elif; ERTÜRK, ELİF; Onur, Ömer Enes; Aydın, İpek; İPEK, AYDIN; Arı, Ferda; ARI, FERDA; Bursa Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Biyoloji Bölümü.; 0000-0001-8707-5918; 0000-0002-6729-7908; IUO-8513-2023; AAG-7012-2021; JQI-3400-2023Breast cancer is the leading cause of cancer-related deaths in women throughout the world. Research on natural anti-cancer products from plants has gained traction. Salvia L. species and their derivatives are rare in Turkey and have suggested for their potential anti-cancer effects. The aim of this study is to assess the potential cytotoxic/apoptotic activities of methanol extract of Salvia candidissima Vahl. subsp. candidissima (SCE) on MCF-7 and MDA-MB-231 breast cancer cells. A GCxGC-TOF/MS system and a dual stage commercial thermal desorption injector were used to determine the chemical components of SCE. MTT and ATP viability tests were used to investigate the anti-growth activity. The apoptosis-inducing effect was assessed using a fluorescence staining method. Caspase-cleaved keratin 18 (ccK18, M30-antigen) levels measured by M30-CytoDeath ELISA Kit. The results showed that SCE suppressed the survival of the MCF-7 and MDA-MB-231 breast cancer cells in a dose-dependent manner, based on the findings of both MTT and ATP cell viability tests and pyknotic cell nuclei were observed via fluorescent staining in both cell lines after 48 h of treatment. The treatment group had greater levels of caspase-cleaved keratin 18 in the MCF-7 cells than the untreated group. These results showed that SCE triggers apoptosis, causes cell death in MCF-7 and MDA-MB-231 cell lines. SCE may become promising therapeutic strategy in the treatment of breast cancer with further in vitro and in vivo studies.Publication New copper(ii) complexes containing tryptophan based schiff bases as promising antiproliferative agents on breast cancer cells(Elsevier, 2023-12-28) Zorlu, Yunus; Arı, Ferda; ARI, FERDA; Gültekin, Büşra; Özbağcı, Duygu Inci; İNCİ ÖZBAĞCI, DUYGU; Aydın, İpek; İPEK, AYDIN; Aydın, Rahmiye; AYDIN, RAHMİYE; Bursa Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Kimya Bölümü.; Bursa Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Biyoloji Bölümü.; 0000-0002-0443-1129; 0000-0002-0483-9642; 0000-0003-4944-0181; 0000-0002-6729-7908; 0000-0003-2811-1872; G-2201-2019; IUO-8513-2023; AAG-7012-2021Three new copper(II) complexes, [Cu(5-ClSal-Trp)(H2O)2] (1), [Cu(5-ClSal-Trp)(phen)] & sdot;C2H5OH (2) and [Cu (3,5-ClSal-Trp)(phen)] (3) (5-ClSal-Trp: Schiff base derived from 5-chlorosalicylaldehyde and L-tryptophan, 3,5-ClSal-Trp: Schiff base derived from 3,5-dichlorosalicylaldehyde and L-tryptophan, phen: 1,10-phenanthroline), have been synthesized and characterized by electronic absorption spectroscopy, CHN analysis, FTIR, ESI-MS and XRD techniques. Interaction of the complexes 1-3 with biomolecules {calf thymus DNA (CT-DNA) and bovine serum albumin (BSA)} has been investigated by electronic absorption and fluorescence spectroscopy. The results show that the complexes 1-3 can bind to CT-DNA via a moderate intercalation mode. Moreover, the fluorescence quenching mechanism between the complexes 1-3 and BSA is a static quenching process. Radical scavenging activity studies reveal that the complexes 1-3 show a moderate activity. Antiproliferative effects of the complexes 1-3 on both breast cancer cells (MCF-7 and MDA-MB-231) and healthy breast epithelial cells (MCF-10A) were also investigated using the Sulforhodamine B (SRB) viability assay. The results demonstrated that the complexes 1-3 exhibited a more pronounced cytotoxic effect on cancer cells compared to normal breast epithelial cells. Among the complexes, the best cytotoxic activity was obtained for the complex 3 against both human breast cancer cell lines. Further analysis indicated that the complex 3 induced apoptosis, as evidenced by fluorescent staining, positive Annexin-V-FITC staining, and the involvement of caspase. Subsequent to the administration of the complex 3, an evaluation of intracellular reactive oxygen species (ROS) generation was conducted through the utilization of dihydroethidium (DHE) fluorescent staining.Publication Changes in ph of digestive tract and cecal microflora composition in broilers fed with probiotic and prebiotic supplementation, synerAll.(Oxford Univ Press Inc, 2016-10-01) İpek, Aydın; Sözcü, Arda; İPEK, AYDIN; SÖZCÜ, ARDA; Uludağ Üniversitesi/Ziraat Fakültesi/Zootekni Bölümü; FCM-0798-2022; HXQ-7280-2023Publication Effect of hatch weight on performance of Japanese Quails (Coturnix coturnix japonica) during growth and egg production period(Eugen Ulmer Gmbh Co, 2004-12-01) İpek, Aydın; Şahan, Ümran; Yılmaz, Bilgehan; İPEK, AYDIN; ŞAHAN, ÜMRAN; YILMAZ DİKMEN, BİLGEHAN; Uludağ Üniversitesi/Hayvancılık Bilimleri Bölümü; 0000-0002-4397-5428; AAH-2966-2021; AAH-2551-2021; G-3725-2017Japanese Quails were seperated into 3 weight groups depending on hatching weight (1(st) group 5.5-6.2 g, 2(nd) group 6.3-7.0 g and 3(rd) group 7.1-7.8 g). The effect of hatching weight on mean live weight, weight gain, feed consumption and feed conversion during the rearing period was found significant (P < 0.05). The effect of hatching weight on mortality was not significant. Females in each group were placed in cages at the end of the rearing period (5(th) week). The effect of hatching weight on age and weight at sexual maturity was found to be significant (P < 0.01). Mean age of females at sexual maturity was found as 43.6, 42.0 and 41.6 days in the 1(st), 2(nd) and 3(rd) groups, respectively. Sexual maturity weight of females was found as 181.4 +/- 5.12, 194.7 +/- 5.68 and 203.4 +/- 6.94 g, respectively. The highest rate of lay and mean egg weights were obtained with the highest hatch weight group.