Person: ŞAHİN, SALİHA
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ŞAHİN
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SALİHA
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Publication Interaction of probiotic activity, antioxidative capacity, and gamma- amino butyric acid (gaba) in chestnut milk-fortified yogurt(Wiley-hindawi, 2022-11-07) Yılmaz-Ersan, Lutfiye; YILMAZ ERSAN, LÜTFİYE; Şahin, Saliha; ŞAHİN, SALİHA; Özcan, Tülay; ÖZCAN, TÜLAY; Akpınar-Bayizit, Arzu; AKPINAR BAYİZİT, ARZU; Usta-Görgün, Buse; Ciniviz, Melike; Keser, Gökce; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Gıda Mühendisliği Bölümü.; Bursa Uludağ Üniversitesi/Fen Edebiyat Fakültesi/ Kimya Bölümü.; 0000-0002-0223-3807; AAG-8194-2021; AAH-2892-2021As a novel functional dairy product, yogurt was produced from mixtures of skimmed milk and chestnut milk using probiotic culture. The survival of probiotic bacteria, antioxidant capacity, total phenolic content, and gamma- amino butyric acid (GABA) content of the samples were determined. Results showed that sample with chestnut milk 100% contained a higher dry matter, fat, carbohydrate, and caloric value and lower vitamin C, and E contents. Samples had high K, Ca, P, Na, and Na values. Probiotic bacteria were capable of growing well and over the recommended therapeutic value in yogurt with fortified chestnut milk. The yogurt samples showed excellent 2,2 '-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) and 2, 2 '-diphenyl-1-picrylhydrazyl radicals scavenging activities as well as ferric reducing antioxidant power. However, it was found that as the chestnut milk ratio of samples increased, the antioxidant capacity values increased. GABA values of samples varied from 0.31 to 2.43 mg 100 g(-1). According to hierarchical cluster analysis, the samples with 20%, 30%, 40%, and 50% chestnut milk had similar compositional properties. Novelty impact statement The objective of the present study was to compare the nutritional, microbiological, and antioxidative properties of probiotic yogurts manufactured using skimmed milk and chestnut milk. Based on the findings of this study, the fortification of chestnut milk, in general, had positive effects on the nutritional properties of probiotic yogurt. The present study describes that yogurt with chestnut milk could be considered a potential source of antioxidant compounds as well as GABA. Chestnut milk-fortified dairy products could be contributed to ensure maximum functional benefits from these products and present a good alternative for consumers demanding healthier foods.Publication Development of an edible active chitosan film loaded with Nigella sativa L. extract to extend the shelf life of grapes(Elsevier, 2023-04-27) Karkar, Büşra; Patır, İlkyaz; Eyuboğlu, Serenay; Şahin, Saliha; KARKAR, BÜŞRA; Patır, İlkyaz; Eyuboğlu, Serenay; ŞAHİN, SALİHA; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü.; AAH-2892-2021; KGI-2441-2024; IUY-0382-2023; JCW-2327-2023Environmentally friendly approaches such as orientation towards natural products and zero waste, which consumers have started to adopt in recent years, have also started to cause new explores in food packaging. The ability of edible films produced from natural polymers to be functionalized with active ingredients, not generate waste, and be useable as an alternative to traditional food packaging has led us to develop Nigella sativa extract (ethanolic and methanolic) loaded chitosan active edible film. The films were characterized by FTIR, SEM, physical properties, total phenolic content, and total antioxidant capacity, in vitro digestion profiles were examined. The grapes were covered with films and physical changes were examined. The total phenolic content of the ethanolic and methanolic film was 384.73 & PLUSMN; 0.59, and 424.67 & PLUSMN; 14.71 mg GAE/100 g film respectively. The total antioxidant capacity of the ethanolic and methanolic film was 255.85 & PLUSMN; 0.10, 293.72 & PLUSMN; 0.44 mg TE/100 g film respectively. Thickness of ethanolic and methanolic film was 0.30 & PLUSMN; 0.21, 0.36 & PLUSMN; 0.06 mm, solubility 16.84 & PLUSMN; 1.54, 23.22 & PLUSMN; 0.31 (w/ w %), moisture ratio 4.45 & PLUSMN; 0.61, 7.58 & PLUSMN; 1.98 (%), swelling ratio 5.65 & PLUSMN; 2.06, 14.74 & PLUSMN; 0.78 (%), water vapor permeation 2.39 & PLUSMN; 1.63, 4.94 & PLUSMN; 4.45 (x10-10g/ms kPa) respectively. FTIR and SEM analysis showed that preparation of the films with Nigella sativa extract did not cause any change in the structure of chitosan. As a result of the observations, it was seen that the uncovered grapes spoilage and the grapes covered with films were not spoilage indicating that the developed films can be used as an alternative to food packaging.Publication Optimization of formulation and process conditions of chestnut-based functional beverage using response surface methodology(Springer India, 2022-06-13) Usta-Görgün, Buse; Yılmaz-Ersan, Lütfiye; Şahin, Saliha; Usta-Görgün, Buse; YILMAZ ERSAN, LÜTFİYE; ŞAHİN, SALİHA; Bursa Uludağ Üniversitesi/Fen Bilimleri Enstitüsü.; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Gıda Mühendisliği Bölümü.; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü.; AAH-2892-2021; GHG-7570-2022; AAG-8219-2021Response surface methodology was used to optimize the chestnut beverage production under the effect of the independent variables including dilution rate (x(1)), dilution temperature (x(2)), pasteurization time (x(3)) and pasteurization temperature (x(4)). The experiments were based on a central composite design with linear and quadratic models employed to study the combined effects of four independent variables. The responses were selected with functional properties such as antioxidative attributes and total phenolic content. The optimal conditions (x(1), x(2), x(3) and x(4)) determined for development of chestnut based functional beverage were a dilution rate of 25.19 g/100 mL, a dilution temperature of 37.562 degrees C, a pasteurization time of 24.996 min. and a pasteurization temperature of 84.433 degrees C. After comparing the predicted and experimental results, the multi-response surface methodology was more stable with a good correlation for a functional chestnut beverage.Publication Development of galangin-loaded nano-sized polyelectrolyte liposome: Optimization and characterization(Springer, 2023-05-24) Karkar, Büşra; Patır, İlkyaz; Şahin, Saliha; KARKAR, BÜŞRA; Patır, İlkyaz; ŞAHİN, SALİHA; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü; 0000-0001-6547-5558; 0000-0002-3503-2550; AAH-2892-2021; IUY-0382-2023; KGI-2441-2024Galangin is a natural flavonol with high antioxidant properties and has a wide range characteristics of biological activity spectrum. Galangin has low solubility, permeability, and bioavailability, limiting its therapeutic use like many flavonoids. In this study, nano-sized polyelectrolyte liposomes were developed and characterized to overcome the properties that limit the use of galangin. The various parameters (phospholipid/solvent ratio, cholesterol/solvent ratio, time and galangin/solvent ratio) were optimized with a response surface methodology-central composite design to develop liposomes with maximum encapsulation efficiency using the thin-film hydration method. An optimum liposome formulation was developed with 93.77 +/- 0.05% encapsulation efficiency, a spherical large unilamellar vesicle with a size of 485.5 +/- 128.41 nm, and a zeta potential of - 48 +/- 7 mV. The optimum liposome formulation was coated with polyelectrolyte biopolymer chitosan (CH) and gum arabic (GUA) using the layer-by-layer deposition method to improve its stability and drug release profile. The CH-liposome has a size of 208.05 +/- 73.04 nm and a zeta potential of + 40 +/- 5 mV. The GUA-CH-liposome has a size of 266.60 +/- 8.49 nm and a zeta potential of - 6 mV. Fourier transform infrared spectroscopy analysis showed that galangin was encapsulated without disturbing the liposome structure and the polyelectrolyte coated the surface with electrostatic interaction. At the end of the in vitro release study, GUA-CH-liposome released 23.84% of galangin. Regarding stability, drug loading capacity of GUA-CH-liposome, which was 93.77 +/- 0.05% on day 0, changed to 92.72 +/- 0.51% at + 4 degrees C, 93.09 +/- 0.01% at room temperature and 93.15 +/- 0.01% at - 24 degrees C on day 28.Publication Olea europaea leaf extract improves the treatment response of GBM stem cells by modulating miRNA expression(E-century Publishing Corp, 2014-01-01) Tezcan, Gülçin; Tunca, Berrin; Bekar, Ahmet; Budak, Ferah; Şahin, Saliha; Çeçener, Gülşah; Egeli, Ünal; Taşkapılıoğlu, Mevlut Özgür; Kocaeli, Hasan; Tolunay, Şahsine; Malyer, Hulusi; Demir, Cevdet; Tümen, Gülendam; TEZCAN, GÜLÇİN; TUNCA, BERRİN; BEKAR, AHMET; BUDAK, FERAH; ŞAHİN, SALİHA; ÇEÇENER, GÜLŞAH; EGELİ, ÜNAL; TAŞKAPILIOĞLU, MEVLÜT ÖZGÜR; KOCAELİ, HASAN; TOLUNAY, ŞAHSİNE; MALYER, HULUSİ; DEMİR, CEVDET; Tümen, Gülendam; Uludağ Üniversitesi/Tıp Fakültesi/Beyin Cerrahi Kliniği; Uludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji Bölümü; Uludağ Üniversitesi/Tıp Fakültesi/Patholoji Bölümü; 0000-0002-5956-8755; 0000-0002-1619-6680; 0000-0001-7625-9148; 0000-0002-3820-424X; 0000-0001-7904-883X; 0000-0001-5472-9065; 0000-0002-9381-0410; ABA-2005-2020; F-8554-2017; ABX-9081-2022; AFR-1890-2022; F-4657-2014; AAH-3843-2020; AAI-1612-2021; ABI-6078-2020; IZP-9398-2023; AAW-5254-2020; AAP-9988-2020; AAH-2892-2021; ABB-8161-2020; AAH-1420-2021; ABI-6078-2020The stem-like cells of Glioblastoma multiforme (GBM) tumors (GSCs) are one of the important determinants of recurrence and drug resistance. The aims of the current study were to evaluate the anticancer effect of Olea europaea leaf extract (OLE) on GBM cell lines, the association between OLE and TMZ responses, and the effect of OLE and the OLE-TMZ combination in GSCs and to clarify the molecular mechanism of this effect on the expression of miRNAs related to cell death. The anti-proliferative activity of OLE and the effect of the OLE-TMZ combination were tested in the T98G, U-138MG and U-87MG GBM cell lines using WST-1 assay. The mechanism of cell death was analyzed with Annexin V/FITC and TUNEL assays. The effects of OLE on the expression levels of miR-181b, miR-153, miR-145 and miR-137 and potential mRNA targets were analyzed in GSCs using RT-qPCR. OLE exhibited anti-proliferative effects via apoptosis and necrosis in the GBM cell lines. In addition, OLE significantly induced the expression of miR-153, miR-145, and miR-137 and decreased the expression of the target genes of these miRNAs in GSCs (p < 0.05). OLE causes cell death in GBM cells with different TMZ responses, and this effect is synergistically increased when the cells are treated with a combination of OLE and TMZ. This is the first study to indicate that OLE may interfere with the pluripotency of GSCs by modulating miRNA expression. Further studies are required, but we suggest that OLE may have a potential for advanced therapeutic cancer drug studies in GBM.Publication Optimization of ultrasonic-assisted extraction of polysaccharides from Ulva rigida and evaluation of their antioxidant activity(Elsevier, 2023-12-08) Akbal, Azat; Şahin, Saliha; Güroy, Betül; ŞAHİN, SALİHA; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü; AAH-2892-2021Ultrasonic-assisted extraction (UAE) of sulfated polysaccharides from the cell walls of Ulva rigida green macro-algae was performed with a central composite design using the independent variables (pH 2-10, extraction temperature 40-80 degrees C, time 50-130 min and liquid/solid ratio of raw material 15-75 mL/g) with response surface methodology. Optimum conditions were obtained as pH 4.92, extraction temperature 73 degrees C, time 126 min, and the liquid/solid ratio of the raw material as 68 mL/g. Under these optimum conditions, the experimental yield was obtained as 13.22 %, and the results were close and in harmony with the estimated value of 12.91 %. FT-IR results, especially at the peaks corresponding to wave numbers 850 cm-1 and 1256 cm -1, the tension vibrations of sulfated esters in C-O-S and S-O bonds were more clearly observed in the extracted ulvan components. As a result of the SEM images obtained by considering the surface morphology, it was observed that the sulfated polysaccharides from the cell wall were more prominently removed after the lipids were removed and as a result of the precipitation process. While the extracted sulfated polysaccharides showed higher anti-oxidant activity, although the results were close to each other. The total phenolic content was higher in the extracted sulfated polysaccharides.Publication Evaluation of antioxidant properties and phenolic composition of fruit tea infusions(MDPI, 2013-12-01) Şahin, Saliha; ŞAHİN, SALİHA; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü; AAH-2892-2021The popularity of fruit tea is increasing in the world because of its antioxidant properties and attractive taste. The aim of this study was to determine and compare the antioxidant property and phenolic composition of 16 different fruit teas. The antioxidant property and total phenol content of fruit teas depending on the extraction condition (water temperature) were examined using the ABTS (2,2-azinobis[3-ethylbenzothiazoline6-sulphonic acid]) method and the Folin-Ciocalteu method, respectively. The contents of total flavonoid and total anthocyanin of fruit teas was determined by using the UV/Vis spectrophotometric method. The phenolic composition was determined and quantified by using high performance liquid chromatography and photodiode array detection (HPLC-PDA). The highest total phenol content and antioxidant capacity were determined in pomegranate (I). The highest contents of total flavonoid and total anthocyanin were determined in peach (III) and blackberry (I), respectively. Chlorogenic acid, quercetin, myricetin, rutin, rosmarinic acid and ferulic acid were determined in fruit teas. A water temperature of 100 degrees C was the most effective to extract the highest contents of total phenols, total flavonoids, total anthocyanins and the highest antioxidant capacity in 16 different fruit teas. The purpose of this study was to determine the effect of water temperature on the extraction and quantify the various phenolic compounds in fruit teas by HPLC method for industrial application in producing the extracts.Publication Determination of carotenoids in Chlamydomonas reinhardth by multivariate analysis with chromatographic data(Parlar Scientific Publications (P S P), 2019-01-01) Şahin, Saliha; Yüksel, Çiğdem; ŞAHİN, SALİHA; Yüksel, Çiğdem; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü.; AAH-2892-2021; KHL-4329-2024The carotenoid content of microalgae is an important quality parameter for nutritional and biological value. Determination of carotenoid content by spectroscopic and chromatographic methods is expensive and time-consuming. This study deals with the determination of carotenoids (0-carotene, zeaxanthin, neoxanthin, lutein and a-carotene) in Chlamydomonas reinhardtii by chromatography combination with principal component regression (PCR) and partial least squares (PLS) methods. Although, the chromatographic peaks of zeaxanthin and lutein were not separated by high performance liquid chromatography, PCR and PLS methods were employed successfully for the prediction of carotenoids in Chlamydomonas reinhardtii. It was found that the root mean square errors of prediction (RMSEP) for the validation set (r12=0) of PLS of 0-carotene, zeaxanthin, neoxanthin, lutein and a-carotene was 0.0500, 0.4300, 0.0252, 0.2800, and 0.1320, respectively. PLS method was used to improve resolution and quantitative determination of carotenoid overlapped highly in complicated natural samples.