Person: VATAN, ÖZGÜR
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VATAN
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ÖZGÜR
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Publication Evaluation of cytotoxic and genotoxic potential of avobenzone and octocrylene on human skin fibroblast cells(Taylor & Francis Ltd, 2023-12-31) Vatan, Özgür; Sevinç, Duygu; Yılmaz Çelik, Dilek; Aurelie Allounan, A. Carine; Teksoy, Özgün; Oral, Neylan; Hüriyet, Hüzeyfe; Çavaş, Tolga; Çinkılıç, Nilüfer; VATAN, ÖZGÜR; Sevinç, Duygu; Yılmaz Çelik, Dilek; Aurelie Allounan, A. Carine; Teksoy, Özgün; Oral, Neylan; Hüriyet, Hüzeyfe; ÇAVAŞ, TOLGA; ÇİNKILIÇ, NİLÜFER; Fen Edebiyat Fakültesi; Biyoloji Bölümü; 0000-0002-7687-3284; 0000-0003-1620-1918; AAH-5296-2021; ISV-0209-2023; O-7508-2015; JOX-7778-2023; JOR-9588-2023; JOL-2743-2023; EDJ-3505-2022; JOX-5573-2023; CVI-9578-2022; EQD-6980-2022We investigated the cytotoxic and genotoxic effects of the organic UVA filter avobenzone (AVB), and the UVB filter octocrylene (OCT), found alone or in combination in different sunscreen formulations, on normal human skin fibroblast cells (CCD-1118Sk). To achieve this purpose, we used XTT viability assay, single cell gel electrophoresis (comet) assay and reactive oxygen species (ROS) assay. For cytotoxicity, CCD-1118Sk cells were exposed to various concentrations of AVB (32-800 mu M), and OCT (130-6500 mu M). The IC50 values were 100.2 and 1390.95 mu M for AVB and OCT, respectively. IC12.5, IC25 and IC50 concentrations were chosen for genotoxicity testing. The comet assay revealed DNA damage at the two highest concentrations of AVB and all OCT concentrations. The rate of DNA damage was significantly higher than in the control groups. Co-treatment with AVB and OCT increased the level of DNA damage and intracellular oxidative stress compared with AVB and OCT treatment alone. Finally, our study showed that the UVA filter AVB, with the UVB filter OCT, may induce cytotoxic and genotoxic effects on human skin fibroblast cells.Highlights Avobenzone and octocrylene are UV filters used in sunscreen products. Healthy human skin fibroblast cell line CCD-1118Sk was used for cytotoxic and genotoxic assays. Cotreatment with avobenzone and octocrylene caused more severe DNA damage than their treatment alone. Our findings will contribute to the limited number of cytotoxicity and genotoxicity studies and lead to more detailed studies on the use of these two chemicals.Publication Evaluation of in vitro cytotoxic, genotoxic, apoptotic, and cell cycle arrest potential of iron-nickel alloy nanoparticles(MDPI, 2022-09-01) Vatan, Özgür; VATAN, ÖZGÜR; Fen Edebiyat Fakültesi; Biyoloji Bölümü; 0000-0002-7687-3284; O-7508-2015; ISV-0209-2023The use of iron-nickel alloy nanoparticles (Fe-Ni ANPs) is increasing daily in various fields. People are increasingly exposed to these nanoparticles for occupational and environmental reasons. Our study determined some of the effects of Fe-Ni ANP exposure and impacts on human health at the cellular level. The cytotoxic and genotoxic potentials of Fe-Ni ANPs were investigated by XTT, clonogenic, comet, and GammaH2AX analyses using Beas-2B cells. Annexin V, multicaspase, and cell cycle arrest methods were used to understand the apoptotic mechanism of action. The intracellular ROS method was used to determine the primary mechanism that leads to cytotoxic and genotoxic activity. The Fe-Ni ANPs showed cytotoxic activity with the XTT and clonogenic methods: they had genotoxic potential, as demonstrated via genotoxicity methods. It was determined that the cytotoxic effect was realized by the caspase-dependent apoptotic pathway, and the cells were stopped at the G0/G1 stage by Fe-Ni ANPs. Increased intracellular ROS due to Fe-Ni ANPs led to cytotoxic, genotoxic, and apoptotic activity. Potential risks to human health due to Fe-Ni ANPs were then demonstrated at the cellular level.Publication Genotoxic and cytotoxic effects of the aglepristone, a progesteron antagonist, in mid-gestation pregnancy termination in rabbits(Kafkas Univ, Veteriner Fakultesi Dergisi, 2015-03-01) Vatan, Özgür; Bağdaş, Deniz; Çinkılıç, Nilüfer; Wehrend, Axel; Özalp, Gözde Rabia; VATAN, ÖZGÜR; Bagdas, Deniz; ÇİNKILIÇ, NİLÜFER; ÖZALP, RABİA GÖZDE; Fen Edebiyat Fakültesi; Biyoloji Bölümü; 0000-0002-7687-3284; 0000-0002-3595-6286; 0000-0003-4694-6937; ISV-0209-2023; O-7508-2015; AAH-5296-2021; AAE-3607-2019; EOB-5882-2022Aglepristone is an antiprogestin using for pregnancy termination in veterinary medicine. The information about side effects of aglepristone is limited. The aim of the study was to investigate cytotoxicity and genotoxicity of aglepristone in mid-gestation pregnancy termination in rabbits. Fifteen New Zealand White rabbits were used and pregnant does were randomly divided into three groups. Group I (n=5) was treated with saline as the control. The does in group II (n=5) and group III (n=5) were treated with aglepristone (10 mg/kg) on 15th day and 15th-16th days of pregnancy, respectively. The rabbits were sacrificed by guillotine 24 h after last treatment. Bone marrow and blood samples were immediately collected. Cytotoxic and genotoxic potential were tested by micronucleus and Comet assays. No genotoxicity and cytotoxicity were found in micronucleus test with single aglepristone administration. In contrast, two consecutive treatments of aglepristone showed high genotoxic and cytotoxic effects on bone marrow in animals. While comet assay of blood samples did not show any significant difference between groups; the results from comet assay of bone marrow cells showed the single injection of aglepristone did not induce any DNA damage but two injections group increased the DNA damage.Publication Synthesis, structural characterization and anticancer activity of 3(3,5-dinitrobenzoyl)-1H-imidazolidine-2-thione(Natl Inst Science Communication & Policy Research-niscpr, 2022-02-01) Genç, Murat; Keskin, Özlem; Kumbıçak, Zübeyde; Vatan, Özgür; ÇAVAŞ, TOLGA; VATAN, ÖZGÜR; Hüriyet, Huzeyfe; Çavaş, Tolga; Fen Edebiyat Fakültesi; Biyoloji Ana Bilim Dalı; CVI-9578-2022; AAH-3508-2021; ISV-0209-2023The present study describes the synthesis, characterization and in vitro anticancer potential evaluation of a novel 3-(3,5-dinitrobenzoyl)-1H-imidazolidine-2-thione compound. In the first step, structure analysis of the compound has been elucidated by NMR and FT-IR techniques. Theoretical NMR, FT-IR spectra, HOMO and LUMO orbital energies and MEP analyses have been used to determine the activity of the molecule by Gaussian 09 package program using DFT techniques. Furthermore, docking calculations have been performed for the BRCA2 (PDB Code: 3EU7) active side to foresee the possible mechanism of action of the synthesized compound. In the second step of the study, the synthesized compound has been screened for its potential in vitro anticancer activity against MCF-7 human breast cancer cell line using the cell proliferation (XTT), apoptosis, cell cycle arrest and intracellular ROS production assays. The results of XTT test revealed significant dereases in MCF-7 cell viability with the 24h IC50 value of 7.57 mu M. It has been also observed that treatment with the IC50 concentration of the compound can significantly induce apoptosis, intracellular ROS production and G2/M phase arrest in MCF-7 cells.Publication In vitro cytotoxic and genotoxic effects of donkey milk on lung cancer and normal cells lines(Czech Academy Agricultural Sciences, 2019-01-01) Akça, Çetin; YILMAZ, DİLEK; Vatan, Özgür; VATAN, ÖZGÜR; Yılmaz, Dilek; Hüriyet, Huzeyfe; Cinkılıç, Nilufer; ÇİNKILIÇ, NİLÜFER; Çavaş, Tolga; ÇAVAŞ, TOLGA; Fen Edebiyat Fakültesi; 0000-0002-7687-3284; 0000-0001-7269-8493; 0000-0002-3595-6286; 0000-0003-1620-1918; AAH-3508-2021; O-7508-2015; ISV-0209-2023; AAH-5296-2021In vitro cytotoxic and genotoxic effects of donkey milk on cancer (A549) and normal (BEAS-2B) lung cell lines were investigated. The XTT and WST-1 tests as well as clonogenic assays were used to evaluate cytotoxicity. The comet assay and micronucleus test were used as genotoxicity endpoints. Donkey milk showed lower cytotoxic effects against normal lung cell line BEAS-2B in comparison to the tumor cell line A549. Genotoxicity experiments revealed dose dependent increases in the frequencies of micronuclei and single stranded DNA breaks in A549 cells whereas no significant damage was observed in BEAS-2B cells. The results indicate that donkey milk has anti-proliferative and genotoxic effects on lung cancer cells at concentrations which are non-toxic to normal lung cells.Publication In vitro evaluation of electrospun polysaccharide based nanofibrous mats as surgical adhesion barriers(Ege Univ, 2020-01-01) Şafak, Şerife; Vatan, Özgür; VATAN, ÖZGÜR; Cinkılıç, Nilufer; ÇİNKILIÇ, NİLÜFER; Karaca, Esra; KARACA, ESRA; Fen Edebiyat Fakültesi; Tekstil Ana Bilim Dalı; 0000-0002-7687-3284; 0000-0002-3595-6286; 0000-0003-1777-3977; AAH-5296-2021; O-7508-2015; AAS-8480-2020; ISV-0209-2023Post-operative adhesions are one of the most important problems faced by patients and surgeons. In this study, nanofibrous mats were produced as novel surgical adhesion barrier from polysaccharide-based polymers, hyaluronic acid, carboxymethyl cellulose and sodium alginate, via electrospinning. The produced nanofibrous mats were crosslinked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysulfosuccinimide. Furthermore, the morphology, in vitro degradation, cytotoxicity and cell adherence potentials of the nanofibrous mats aimed to be used as adhesion barriers were evaluated and compared with a commercial adhesion barrier. Results of the in vitro experiment showed that the nanofibrous mats have maintained their physical structures during the critical period for adhesion formation, and had non-adherent cell feature and non-cytotoxic nature required for an ideal adhesion barrier.