Person: SARIL, AHMET
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Publication Identification of serum proteins in dogs naturally infected with Anaplasma phagocytophilum and Borrelia burgdorferi: A pilot study(Hellenic Veterinary Medical Soc, 2021-10-01) Martinez, L. F.; Escribano, D.; Schanilec, P.; Martinez-Subiela, S.; Tvarijonaviciute, A.; Ceron, J. J.; Yilmaz, Z.; Kocatürk, M.; Levent, P.; Sarıl, A.; SARIL, AHMET; Cihan, H.; Yilmaz, Zeki; YILMAZ, ZEKİ; Yalcin, E.; YALÇIN, EBRU; Veteriner Fakültesi; 0000-0001-9836-0749; 0000-0002-6697-6079; ; ACP-9956-2022; A-9637-2008; ACP-9956-2022Serum proteomic analysis would aid in better understanding the pathophysiology of several diseases. The aim of this study was to identify the serum proteomes of dogs with anaplasmosis and Lyme disease using a pro-teomic approach. Diseases were diagnosed by a commercial rapid in-clinic ELISA. Borrelia antibodies were evaluated by IFAT. Four groups were designated: symptomatic dogs with anaplasmosis (n=5), dogs with Lyme disease (n=5), dual-positive dogs (n=5), and healthy control dogs (n=5). Serum samples were collected before treatment. Two-dimen-sional electrophoresis of pooled samples in each group were run in triplicate. Ten out of 57 differentially expressed spots between groups were evaluated for identification by mass spectrometry. Compared to those of controls, levels of vitamin D-binding protein (VDBP), glycoprotein-9 (GP9) and kininogen-1 (KGN-1) decreased, while haptoglobin (Hp) and immunoglobulin (Ig) heavy chain levels increased in dual infection group. Serum apolipoprotein-A1 (Apo-A1) levels decreased in dogs with anaplasmosis, Lyme disease and dual in-fections compared to those in control dogs. Serum clusterin levels decreased in dogs with anaplasmosis but were not differentially expressed in dogs with Lyme disease or dogs with dual infections compared to those in control dogs. Calpain-3 decreased in dogs with anaplasmosis and Lyme disease. This study showed that many protein levels might be changed in dogs with naturally acquired anaplasmosis and Lyme disease. Understanding the role of these proteins in different biological processes can provide information of interest for diagnostic and therapeutic approaches for these clinical conditions.Publication Effect of pimobendan in the treatment of pulmonary hypertension in a cat with giant right atrium(Kafkas Univ, Veteriner Fakultesi Dergisi, 2020-09-01) Tanaka, Ryou; Levent, Pınar; Sarıl, Ahmet; SARIL, AHMET; Kocatürk, Meriç; KOCATÜRK, MERİÇ; Yilmaz, Zeki; YILMAZ, ZEKİ; Veteriner Fakültesi; Dahiliye Ana Bilim Dalı; 0000-0002-2849-1222; 0000-0001-9836-0749; V-5578-2017; A-9637-2008Publication Serum proteomic changes in dogs with different stages of chronic heart failure(Mdpi, 2022-02-01) Sarıl, Ahmet; Kocatürk, Meriç; Shimada, Kazumi; Uemura, Akiko; Akgün, Emel; Levent, Pınar; Baykal, Ahmet Tarık; Prieto, Alberto Munoz; Agudelo, Carlos Fernando; Tanaka, Ryou; Ceron, Jose Joaquin; Koch, Jorgen; Yılmaz, Zeki; SARIL, AHMET; KOCATÜRK, MERİÇ; Levent, Pınar; YILMAZ, ZEKİ; Veteriner Fakültesi; İç Hastalıkları Ana Bilim Dalı; 0000-0001-9836-0749 ; T-4585-2019; V-5578-2017; DDR-1481-2022; A-9637-2008Simple Summary Canine MMVD is a progressive chronic disease with variable clinical signs, with some patients remaining completely asymptomatic while others develop CHF. Here, the aims of the pilot study were to evaluate serum proteins by proteomic analysis in dogs at different stages of chronic heart failure (CHF) due to degenerative mitral valve disease (MMVD), and how these proteins can change after a conventional treatment. Study revealed 157 different proteins; 11 were up- and 21 down-regulated at a statistically significant level in dogs with CHF compared to controls. Based on the bioinformatic analysis, protein-protein interactions between complement proteins, fibrinogen subtypes and others (albumin precursor, serpins, inter-alpha-trypsin inhibitor heavy chain, fetuin, clusterin, apolipoproteins, and alpha-glycoproteins) showed that pathophysiology of CHF seems to be more sophisticated than we had thought. These proteins are associated with several cellular, biologic, and metabolic processes such as immune-inflammatory responses, hemostasis, oxidative stress, and energy metabolism, which might be detrimental in the progression of canine CHF. Their molecular and biological functions as well as roles in the signaling pathways, such as inflammation, cadherin signaling, nicotinic acetylcholine receptor signaling and Wnt signaling make them possible biomarkers and therapeutic targets for the diagnosis and treatments in dogs with different stages of CHF. MMVD, the most common cause of CHF in dogs, is a chronic disease with variable clinical signs, with some patients remaining asymptomatic while others develop CHF. Here, we aimed to evaluate serum proteins by proteomic analysis in dogs at different stages of CHF due to MMVD, and proteome behaviors after conventional treatment. A total of 32 dogs were divided equally into four groups-stage A (healthy/controls), stage B2 (asymptomatic), stage C and stage D (symptomatic)-according to the ACVIM consensus. Serum proteomes were evaluated using LC/MS-based label-free differential proteome analysis. The study revealed 157 different proteins; 11 were up- and 21 down-regulated in dogs with CHF compared to controls. In stage B2 dogs, angiotensinogen (AGT) was up-regulated, but immunoglobulin iota chain-like, lipopolysaccharide-binding protein, and carboxypeptidase (CPN) were down-regulated. In stage C dogs, complement C3 (C3) and inter-alpha-trypsin inhibitor heavy chain were up-regulated, but hemopexin, and actin-cytoplasmic-1 (ACT-1) were down-regulated. In stage D dogs, AGT was up-regulated, whereas tetranectin, paraoxonase-1, adiponectin and ACT-1 were down-regulated. A decrease in CPN, C3 and AGT and an increase in ACT-1 were observed after treatment of dogs in stage C. This pilot study identified that dogs at different stages of CHF show different serum protein composition which has potential to be biomarker for diagnose and treatment monitorization.Publication Ventricular septal defect and pulmonic stenosis in a dog(Kafkas Univer, Veteriner Fakultesi Dergisi, 2019-09-01) Uemura, Akiko; Tanaka, Ryou; Yılmaz, Zeki; YILMAZ, ZEKİ; Levent, Pınar; Sarıl, Ahmet; SARIL, AHMET; Kocatürk, Meriç; KOCATÜRK, MERİÇ; 0000-0001-9836-0749; 0000-0003-2671-5074; 0000-0002-2849-1222; 0000-0001-9948-6490; V-5578-2017; M-3078-2019; T-1481-2019; A-9637-2008