Person: ÖZARDA, YEŞİM
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Publication Stability of hematological analytes during 48 hours storage at three temperatures using cell-dyn hematology analyzer(Soc Medical Biochemists Serbia, 2021-01-01) Özmen, Sevda Ünalli; Özarda, Yeşim; ÖZARDA, YEŞİM; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyokimya Anabilim Dalı; AAL-8873-2021Background: The complete blood count (CBC) with differential leukocyte count (DLC) is one of the most common tests requested by physicians. The results of this test are affected by storage temperature and time of incubation. This study was designed to evaluate the stability of hematologic parameters in blood specimens stored for 48 h at three temperatures.Methods: K-2-EDTA - blood was collected from 22 healthy adults. The CBC was performed using a hematology analyser immediately; 0 time point and at 4, 8, 12, 16, 20, 24, and 48 h after storage at 4 degrees C, 10 degrees C or 23 degrees C. Changes in values of CBC parameters from the 0 time point were determined and reported as % of the initial value.Results: Red blood cells, platelet, hemoglobin, and mean corpuscular hemoglobin were found stable during 48 h storage at 4 degrees C, 10 degrees C or 23 degrees C. Hematocrite and mean corpuscular volume increased, while white blood cells decreased at 48 h when stored at 23 degrees C. Lymphocytes, neutrophils, eosinophils, and basophils showed significant differences after 12 h of storage at 23 degrees C.Conclusions: Red blood cells, platelet, hemoglobin, and mean corpuscular hemoglobin are the only suitable parameters without refrigeration during 24 h storage. When CBC and DLC are performed, 4 degrees C can be recommended as the most suitable storage temperature for 12 h storage.Publication Relationship with excessive daytime sleepiness and serum substance P levels in OSAS patients and the effect of PAP treatment(Springer Japan, 2019-07-01) Güçlu, Özge Aydın; Ursavaş, Ahmet; Kasapoğlu, Fikret; Özarda, Yeşim; Bozyiğit, Cengiz; Ocakoğlu, Gökhan; Karadağ, Mehmet; AYDIN GÜÇLÜ, ÖZGE; URSAVAŞ, AHMET; KASAPOĞLU, FİKRET; ÖZARDA, YEŞİM; BOZYİĞİT, CENGİZ; OCAKOĞLU, GÖKHAN; KARADAĞ, MEHMET; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Göğüs Hastalıkları Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Kulak Burun Boğaz Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyokimya Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Biyoistatistik Anabilim Dalı.; 0000-0003-1005-3205; 0000-0003-2215-6973; 0000-0002-1114-6051; 0000-0002-9027-1132; AAI-3877-2021; AAI-3169-2021; AAH-5180-2021; AAL-8873-2021; HLG-6346-2023; AAG-8744-2021; AAG-9930-2019; AAE-1623-2022Obstructive sleep apnea syndrome (OSAS) is a commonly seen disorder characterized by repeated episodes of upper airway obstruction during sleep leading to intermittent hypoxemia or arousal. We aim to evaluate the effects of positive airway pressure (PAP) treatment on daytime sleepiness and serum Substance P (SP) levels in OSAS patients. Seventy-one consecutive patients with newly diagnosed OSAS and 19 non-apneic control subjects were enrolled to the study. PAP treatment indicated subjects were re-evaluated after 3 months of treatment. Morning SP levels of OSAS patients and Epworth sleepiness scale (ESS) were assessed at the beginning and then after 3 months of PAP treatment. Of the patients 71 (78.9%) were male and 19 (21.1%) were female, with a median age of 45 [20-62]. The levels of SP in the OSAS group were significantly lower than the snorer group and a significant correlation was not found between serum levels of SP and ESS. SP levels were negatively correlated with AHI. The baseline SP median was 336.1pg/mL [121.6-536.1], while the 3rd month SP median was 213.1pg/mL [103.5-727.6]. Serum SP values were found to have significantly decreased at 3months (p<0.0001). Statistically significant correlation was not found between percentage of ESS change and the percentage of SP change. It can be assumed that the SP level is reduced as part of the compensation mechanism in OSAS cases and supporting this mechanism, the PAP therapy further reduces the SP value by relieving the cases from apnea and the intermittent hypoxia burden.Publication Serum leptin and ghrelin levels and their relationship with serum cortisol,thyroid hormones, lipids, homocysteine and folic acid in dogs with compulsive tail chasing(Cabi Publishing-c A B Int, 2017-01-01) Yalçın, Ebru; Yılmaz, Zeki; Özarda, Yeşim; Denenberg, S; YALÇIN, EBRU; YILMAZ, ZEKİ; ÖZARDA, YEŞİM; Uludağ Üniversitesi/Veteriner Fakültesi/Dahiliye Bölümü; Denenberg, S; 0000-0001-9836-0749; 0000-0002-6697-6079; AAL-8873-2021; ACP-9956-2022; A-9637-2008Publication A multicenter nationwide reference intervals study for common biochemical analytes in turkey using abbott analyzers(Walter De Gruyter Gmbh, 2014-12-01) Ichihara, Kiyoshi; Aslan, Diler; Aybek, Hulya; Ari, Zeki; Taneli, Fatma; Coker, Canan; Akan, Pinar; Şişman, Ali Riza; Bahçeci, Onur; Sezgin, Nurzen; Demir, Meltem; Yücel, Gultekin; Akbas, Halide; Özdem, Sebahat; Polat, Gürbüz; Erbağci, Ayse Binnur; Örkmez, Mustafa; Mete, Nuriye; Evliyaoglu, Osman; Kıyıcı, Aysel; Vatansev, Hüsamettin; Ozturk, Bahadir; Yucel, Dogan; Kayaalp, Damla; Dogan, Kubra; Pinar, Asli; Gurbilek, Mehmet; Çetinkaya, Çiğdem Damla; Akın, Okhan; Serdar, Muhittin; Kurt, Ismail; Erdinç, Selda; Kadiceşme, Özgur; Ilhan, Necip; Atali, Dilek Sadak; Bakan, Ebubekir; Polat, Harun; Noyan, Tevfik; Can, Murat; Bedir, Abdulkerim; Okuyucu, Ali; Değer, Orhan; Agaç, Suret; Ademoğlu, Evin; Kaya, Aysem; Nogay, Turkan; Eren, Nezaket; Gunes, Yeliz; Kawano, Reo; Tezcan, Sehavet; Demirpençe, Özlem; Değirmen, Elif; Özarda, Yesim; ÖZARDA, YEŞİM; Dirican, Melahat; DİRİCAN, MELEHAT; Tuncer, Gül Özlem; Aykuş, Mehmet; Özmen, Sevda Ünalli; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; KYR-0994-2024; AAL-8873-2021Background: A nationwide multicenter study was organized to establish reference intervals (RIs) in the Turkish population for 25 commonly tested biochemical analytes and to explore sources of variation in reference values, including regionality.Methods: Blood samples were collected nationwide in 28 laboratories from the seven regions (>= 400 samples/region, 3066 in all). The sera were collectively analyzed in Uludag University in Bursa using Abbott reagents and analyzer. Reference materials were used for standardization of test results. After secondary exclusion using the latent abnormal values exclusion method, RIs were derived by a parametric method employing the modified Box-Cox formula and compared with the RIs by the non-parametric method. Three-level nested ANOVA was used to evaluate variations among sexes, ages and regions. Associations between test results and age, body mass index (BMI) and region were determined by multiple regression analysis (MRA).Results: By ANOVA, differences of reference values among seven regions were significant in none of the 25 analytes. Significant sex-related and age-related differences were observed for 10 and seven analytes, respectively. MRA revealed BMI-related changes in results for uric acid, glucose, triglycerides, high-density lipoprotein (HDL)-cholesterol, alanine aminotransferase, and.-glutamyltransferase. Their RIs were thus derived by applying stricter criteria excluding individuals with BMI >28 kg/m(2). Ranges of RIs by non-parametric method were wider than those by parametric method especially for those analytes affected by BMI.Conclusions: With the lack of regional differences and the well-standardized status of test results, the RIs derived from this nationwide study can be used for the entire Turkish population.Publication Bone mineral density and the influencing factors in the survivors of childhood all(Blackwell Publishing, 2008-04-01) Can, E.; SAĞLAM, HALİL; Güneş, A. M.; Özarda, Yeşim; ÖZARDA, YEŞİM; Sağlam, Halil; Baytan, B.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Pediatri Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; 0000-0003-1214-0177; 0000-0002-6598-8262; 0000-0002-9375-2855; AAL-8873-2021; C-7392-2019Publication Homocysteine levels in patients with obstructive sleep apnea syndrome(Turkish Assoc Tuberculosis & Thorax, 2008-01-01) İlcöl, Yesim Ozarda; ÖZARDA, YEŞİM; Yavuz, Zekiye; Ege, Ercüment; Ursavaş, Ahmet; URSAVAŞ, AHMET; Gözü, R. Oktay; Uzaslan, Esra; KARADAĞ, MEHMET; UZASLAN, AYŞE ESRA; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Göğüs Hastalıkları Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; 0000-0002-9027-1132; AAL-8873-2021; AAI-3169-2021; AAG-8744-2021; AAI-1004-2021The exact mechanism of development of cardiovascular disease in patients with obstructive sleep apnea syndrome (OSAS) remains to be unknown. The role of homocystein in atherosclerotic disease process has become well established over the past ten years. Our aim was to study to compare homoscysteine levels between OSAS and control levels. Sixty-two subjects with OSAS and twelve similar controls in age, gender, body mass index, smoking and coronary heart disease were included in this prospective study. Serum levels of homocysteine (13.5 +/- 6.0 mu mol/L vs. 10.2 +/- 2.9 mu mol/L, p= 0.03) in the OSAS group were significantly greater than those in the control group. Logistic regression analyses showed that OSAS ( Odds ratio: 9.08 95% CI 2.347-35.120; p= 0.001) was independent risk factors for high levels of serum homocysteine in age, smoking status, diabetes mellitus and coronary heart disease. We conclude that homocysteine may be an important factor for development of cardiovascular disease in patients with OSAS.Publication Big data and reference intervals: Rationale, current practices, harmonization and standardization prerequisites and future perspectives of indirect determination of reference intervals using routine data(Walter De Gruyter Gmbh, 2021-03-10) Martinez-Sanchez, Luisa; Marques-Garcia, Fernando; Blanco, Albert; Brouwer, Nannette; Canalias, Francesca; Cobbaert, Christa; Thelen, Marc; den Elzen, Wendy; Özarda, Yeşim; ÖZARDA, YEŞİM; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyokimya Anabilim Dalı.Reference intervals are commonly used as a decision-making tool. In this review, we provide an overview on "big data " and reference intervals, describing the rationale, current practices including statistical methods, essential prerequisites concerning data quality, including harmonization and standardization, and future perspectives of the indirect determination of reference intervals using routine laboratory data.Publication Leptin concentration in breast milk and its relationship to duration of lactation and hormonal status(Bmc, 2006-01-01) İlcöl, Yeşim Özarda; ÖZARDA, YEŞİM; Hızlı, Z. Banu; HIZLI, MEFAİL; Özkan, Tanju; ÖZKAN, TANJU MUNEVVER; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Pediatri Anabilim Dalı.; 0000-0001-5740-9729; AAL-8873-2021Background: Leptin, a hormone present in breast milk, is involved in energy regulation and metabolism. The objectives of this study were to assess leptin concentrations in breast milk during the first 180 days postpartum, and to determine the relationship between the concentrations of milk leptin and circulating hormone levels in lactating women.Methods: Between April 2005 and January 2006, blood and breast milk samples were collected from 160 breastfeeding women enrolled either in the first three days (n = 37; colostrum), days 414 (n = 27; transitional milk), days 15-30 (n = 16; early mature milk), days 31-90 (n = 37; mature milk) or days 91-180 (n = 43; late mature milk) postpartum. Milk and serum leptin levels were measured by immunoradiometric assay. Cortisol was measured by radioimmunoassay method. Serum insulin, estradiol, prolactin and thyroxine were measured by chemiluminescent immunometric method.Results: Leptin concentrations in breast milk were highest (3.28 +/- 0.41 ng/ml) in colostrum, decreased during the first 180 days of lactation, showing a significant inverse relation (r = -0.694, p < 0.001) with the days of lactation. Colostrum leptin concentrations correlated with maternal serum leptin (r = 0.425, p < 0.01), cortisol (r = 0.549, p < 0.01) and thyroxine (r = -0.530, p < 0.01). Mature milk leptin concentrations correlated with maternal serum leptin (r = 0.547, p < 0.001), insulin (r = 0.331, p < 0.05) and thyroxine ( r = -0.329, p < 0.01). Serum leptin concentrations correlated with serum insulin (r = 0.648, p < 0.001), estradiol (r = 0.639, p < 0.001), prolactin (r = -0.530, p < 0.001) and thyroxine (r = -0.327, p < 0.05) concentrations during days 1-3 postpartum. During 15-180 postpartum days, serum leptin concentrations correlated with serum insulin (r = 0.271, p < 0.01), and thyroxine (r = -0.345, p < 0.001).Conclusion: Leptin concentrations in breast milk decrease with time during lactation and show significant relationships with other maternal hormones.