Person: İPEK, AHMET
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Publication Development of aflp markers associated with zucchini yellow mosaic virus resistance in cucumber (Cucumis sativus L.)(Tübitak Bilimsel ve Teknolojik Araştırma Kurumu, 2015-01-01) Sigva, Hasan Ozgur; Fırat, Ahmet Fikret; Hazarhun, Gulden; İpek, Ahmet; İPEK, AHMET; Ziraat Fakültesi; Bahçe Bitkileri Bölümü; 0000-0001-5821-2426; AAK-4655-2021Zucchini yellow mosaic virus (ZYMV) is one of the most important pathogens that cause significant yield losses in many cucurbit crops including cucumber (Cucumis sativus L). ZYMV resistance in cucumber is inherited by a single recessive gene. The purpose of this study was to identify molecular markers linked to the gene conferring ZYMV resistance in cucumber. We developed a population of 188 F-2 plants derived from inbred cucumber lines. Individual F-2 plants were self-pollinated to generate F-3 populations. Ten randomly selected plants from each F-3 population were tested for ZYMV resistance. We used a bulk segregant analysis method to identify putative molecular markers linked to ZYMV resistance. Using bulked DNA samples with parental lines and F-1, a total of 170 sequence-related amplified polymorphism (SRAP), 586 simple sequence repeat (SSR), and 308 amplified fragment length polymorphism (AFLP) primer combinations were screened. Neither polymorphic SRAP nor SSR markers were linked with ZYMV resistance. Among the 308 AFLP primer combinations tested, an AFLP marker in the E-ACA/MCA primer combination showed significant association among parental lines, F-1, and resistant and susceptible plants. The combination of E-ACA/M-CA was achieved on parental lines, F-1, and 188 F-2 individuals for confirmation of the marker segregation on the F-2 population. We found that the combination of E-ACA/M-CA was linked to the zym locus with 6.91 cM.Publication Determination of hybrid potential of carrot germplasm(Int Soc Horticultural Science, 2019-01-01) Lokoğlu, N.; Gökce, A. F; İpek, A.; İPEK, AHMET; Ziraat Fakültesi; Gokce, AFThe use of the seed of hybrid cultivars for carrot production in Turkey has reached to 90% and this hybrid carrot cultivar seed has been imported from abroad in Turkey, 2 t of seeds from open-pollinated cultivars and 18 t of seeds from hybrid cultivars were used for carrot production. While the seed of open-pollinated cultivars was sold for 150-200 TL kg(-1), the price of one kg of seed of hybrid cultivars was 7501,500 TL. Hybrid cultivars were preferred by producers due to the above mentioned reasons. The reasons why hybrid cultivars have been preferred for carrot production are high yield and uniform root size, shape, color and taste. In this research project, potential to convert open pollinated cultivars collected from Turkey and abroad to hybrid cultivars will be determined. Petalloid cytoplasmic male sterility has been preferred because it is easy to observe male sterility from the morphology of carrot flowers. Therefore, for hybrid seed production, maintainer lines have to be identified to propagate male sterile female parent. This Project began at 2013 in Transitional Zone Agricultural Research Institute. This is a project of the General Directorate of Agricultural Research and Policies.Publication Genetic variation and relationships between Azerbaijani and Turkish olive genetic resources(Springer, 2022-06) Duran, Sevin Teoman; Aghayeva, Saltanat; Akparov, Zeynal; Mammadov, Ayaz; Asgarova, Rana; Uslu, Osman Yaşar; Kırıkoğlu, Osman; Duran, Ufuk Tan; İpek, Meryem; Barut, Erdogan; Ercişli, Sezai; İlhan, Gülce; İpek, Ahmet; TEOMAN DURAN, SEVİN; İPEK, MERYEM; İPEK, AHMET; BARUT, ERDOĞAN; Kırıkoğlu, Osman; Duran, Ufuk Tan; Karacabey Meslek Yüksekokulu; Süt ve Besi Hayvancılığı Bölümü; Organik Tarım Programı; 0000-0003-1469-6777; 0000-0001-5821-2426; AAE-4675-2019; AAH-3233-2021; AAK-4655-2021; AAE-6913-2019; FCC-3703-2022; IDH-4027-2023Olive (Olea europaea L.) is one of the most economically important crop from east to the west around the world. The aim of this research was to investigate the genetic relationship among 41 olive genotypes, including 11 well-known Turkish cultivars and 30 Azerbaijani olive genotypes using simple sequence repeat (SSR) markers. In this study, 19 SSR markers were amplified 115 polymorphic SSR alleles. The number of polymorphic alleles ranged from 3 to 10 with an average of 6.05. The observed heterozygosity (Ho) varied from 0.05 to 0.93 with an average of 0.63 and expected heterozygosity (He) differed from 0.26 to 0.86 with an average of 0.72. The polymorphism information content (PIC) ranged from 0.23 to 0.85 with a mean of 0.68. A UPGMA cluster analysis grouped olive genotypes into two distinct clusters and both clusters were divided into two subgroups. Similarly, STRUCTURE analysis assigned olive genotypes into two different gene pools (K = 2) and four gene pools were identified representing the two subgroups by STRUCTURE analysis for K = 4. The genetic similarity of olive genotypes ranged from 0.36 to 0.95. These results revealed that there was a high genetic variation among 30 Azerbaijani olive genotypes. 'Ayvalik 1'and 'Ayvalik 2' from Azerbaijani olive genotypes were different from Turkish local olive cultivar, "Ayvalik" indicating homonymy. This research also highlighted that Azerbaijani olive genotypes were totally distinct from Turkish olive cultivars demonstrating that these olive genotypes might have been imported to Azerbaijan from different countries other than Turkey. The outcomes of this study indicated that these diverse olive genotypes could be useful for development of new olive varieties in Azerbaijan and future breeding programs between two countries could be enhanced by means of these results.Publication Characterization of phytochemical and functional properties of endemic edible Allium tuncelianum and comparison with commonly cultivated garlic, onion and leek crops(Springer, 2023-01-13) İPEK, MERYEM; ŞAHAN, YASEMİN; CANSEV, ASUMAN; Cansev, Asuman; İPEK, AHMET; Ipek, Ahmet; Altınseker Acun, Deniz Zahide; Ziraat Fakültesi; AAH-3233-2021; ABE-6748-2020; AAH-4255-2019; AAK-4655-2021Edible Allium tuncelianum ([Kollman] Ozhatay, Matthew and Siraneci), endemic to Turkey, has received increased attention in recent years because of its appealing characteristics, such as garlic (A. sativum L.)-like odor and single-clove solid bulb structure with few peels, which could be advantageous in the food processing industry. The aims of this study were to determine chemical composition, phytochemicals (alliin, allicin, phenolics), antioxidant capacity, and bioaccessibility of total phenolic content and antioxidant capacity in A. tuncelianum and to compare with the values in commonly cultivated Allium species: garlic, onion (A. cepa L.), and leek (A. porrum L.) to reveal its nutritional and health beneficial properties. The chemical composition of A. tuncelianum was comparable with that of garlic, and its dietary fiber content was three times higher than that of garlic. Alliin and allicin organosulfur compounds, which give garlic its distinct flavor and odor, were detected in all analyzed A. tuncelianum genotypes although their values lower than the amounts detected in garlic genotypes. However, alliin and allicin were not detected in any onion and leek genotypes. A. tuncelianum genotypes had higher total phenolic contents with over 78 percent bioaccessibility rates than garlic, onion, and leek. The antioxidant capacity of A. tuncelianum was comparable to those of cultivated Allium species. Our results suggested that A. tuncelianum can be considered a promising new health-enhancing crop for human nutrition.(GRAPHICS)Publication Selection of salt tolerant lines at cell level using gamma ray with callus and suspension culture techniques in black carrots (Daucus carota L. ssp. sativus var. atrorubens Alef.)(Pergamon-elsevier Science Ltd, 2022-10-27) Büyükdinç, Damla Turan; Kantoğlu, K. Yaprak; Kuşvuran, Şebnem; İpek, Ahmet; Karataş, Arzu; Ellialtıoğlu, Şebnem; İPEK, AHMET; Ziraat Fakültesi; Bahçe Bitkileri Bölümü; 0000-0001-5821-2426 ; AAK-4655-2021The main objective was to select salt tolerant lines at the cell level of Hatay region's black carrot (Daucus carota L. ssp. sativus var. atrorubens Alef.) using callus and suspension culture techniques combined with gamma rays. Hypocotyl explants of in vitro grown plantlets was used for callus induction. Effective mutation dose was determined by gamma radiation treatment at various doses (0, 5, 10, 20, 30, 40, 50, 60 Gy) to black carrot calli after in vitro optimization steps. According to regression analysis, the number of plants regenerated from calli was found 8.36 Gy as effective dose. In the ongoing study, calli with 7 Gy, 8 Gy and 9 Gy gamma rays were multiplied by subculture for 5 times. Shoot induction was achieved in medium containing 1 mg L-1 BAP concentration. Average plant height, root length and branching number parameters of plants regenerated from calli were determined. Salt stress was applied to the plants acclimatized from in vitro to the climate chambers. changes in the amount of peroxidase (POD) and superoxide dismutase (SOD) activities of antioxidant enzymes and the changes in lipid peroxidation were revealed in leaf samples taken from plants that continued to live in a salty environment after the 14 days of the treatment. At the end of the study, salt tolerance increased in mutant plants have the plant number of 8-21, 9-19, 7-9, 9-2 and 9-8 compared to the control, and these were determined as possible mutant plants.