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İPEK, AHMET

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İPEK

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AHMET

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2015 - 201912020 - 20221
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    Publication
    Genetic variation and relationships between Azerbaijani and Turkish olive genetic resources
    (Springer, 2022-06) Duran, Sevin Teoman; Aghayeva, Saltanat; Akparov, Zeynal; Mammadov, Ayaz; Asgarova, Rana; Uslu, Osman Yaşar; Kırıkoğlu, Osman; Duran, Ufuk Tan; İpek, Meryem; Barut, Erdogan; Ercişli, Sezai; İlhan, Gülce; İpek, Ahmet; TEOMAN DURAN, SEVİN; İPEK, MERYEM; İPEK, AHMET; BARUT, ERDOĞAN; Kırıkoğlu, Osman; Duran, Ufuk Tan; Bursa Uludağ Üniversitesi/Karacabey Meslek Yüksekokulu/Süt ve Besi Hayvancılığı Bölümü/Organik Tarım Programı; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü; 0000-0003-1469-6777; 0000-0001-5821-2426; AAE-4675-2019; AAH-3233-2021; AAK-4655-2021; AAE-6913-2019; FCC-3703-2022; IDH-4027-2023
    Olive (Olea europaea L.) is one of the most economically important crop from east to the west around the world. The aim of this research was to investigate the genetic relationship among 41 olive genotypes, including 11 well-known Turkish cultivars and 30 Azerbaijani olive genotypes using simple sequence repeat (SSR) markers. In this study, 19 SSR markers were amplified 115 polymorphic SSR alleles. The number of polymorphic alleles ranged from 3 to 10 with an average of 6.05. The observed heterozygosity (Ho) varied from 0.05 to 0.93 with an average of 0.63 and expected heterozygosity (He) differed from 0.26 to 0.86 with an average of 0.72. The polymorphism information content (PIC) ranged from 0.23 to 0.85 with a mean of 0.68. A UPGMA cluster analysis grouped olive genotypes into two distinct clusters and both clusters were divided into two subgroups. Similarly, STRUCTURE analysis assigned olive genotypes into two different gene pools (K = 2) and four gene pools were identified representing the two subgroups by STRUCTURE analysis for K = 4. The genetic similarity of olive genotypes ranged from 0.36 to 0.95. These results revealed that there was a high genetic variation among 30 Azerbaijani olive genotypes. 'Ayvalik 1'and 'Ayvalik 2' from Azerbaijani olive genotypes were different from Turkish local olive cultivar, "Ayvalik" indicating homonymy. This research also highlighted that Azerbaijani olive genotypes were totally distinct from Turkish olive cultivars demonstrating that these olive genotypes might have been imported to Azerbaijan from different countries other than Turkey. The outcomes of this study indicated that these diverse olive genotypes could be useful for development of new olive varieties in Azerbaijan and future breeding programs between two countries could be enhanced by means of these results.
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    Publication
    Development of aflp markers associated with zucchini yellow mosaic virus resistance in cucumber (Cucumis sativus L.)
    (Tübitak Bilimsel ve Teknolojik Araştırma Kurumu, 2015-01-01) Sigva, Hasan Ozgur; Fırat, Ahmet Fikret; Hazarhun, Gulden; İpek, Ahmet; İPEK, AHMET; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü; 0000-0001-5821-2426; AAK-4655-2021
    Zucchini yellow mosaic virus (ZYMV) is one of the most important pathogens that cause significant yield losses in many cucurbit crops including cucumber (Cucumis sativus L). ZYMV resistance in cucumber is inherited by a single recessive gene. The purpose of this study was to identify molecular markers linked to the gene conferring ZYMV resistance in cucumber. We developed a population of 188 F-2 plants derived from inbred cucumber lines. Individual F-2 plants were self-pollinated to generate F-3 populations. Ten randomly selected plants from each F-3 population were tested for ZYMV resistance. We used a bulk segregant analysis method to identify putative molecular markers linked to ZYMV resistance. Using bulked DNA samples with parental lines and F-1, a total of 170 sequence-related amplified polymorphism (SRAP), 586 simple sequence repeat (SSR), and 308 amplified fragment length polymorphism (AFLP) primer combinations were screened. Neither polymorphic SRAP nor SSR markers were linked with ZYMV resistance. Among the 308 AFLP primer combinations tested, an AFLP marker in the E-ACA/MCA primer combination showed significant association among parental lines, F-1, and resistant and susceptible plants. The combination of E-ACA/M-CA was achieved on parental lines, F-1, and 188 F-2 individuals for confirmation of the marker segregation on the F-2 population. We found that the combination of E-ACA/M-CA was linked to the zym locus with 6.91 cM.