Person: ERDOST, HATİCE
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ERDOST
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HATİCE
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Publication Toxicity assessment of chronic exposure to common insecticides and bee medications on colony development and drones sperm parameters(Springer, 2021-04-12) Ben Abdelkader, Faten; Çakmak, İbrahim; Çakmak, Selvinar Seven; Nur, Zekariya; İncebıyık, Ece; Aktar, Ahmet; Erdost, Hatice; Ben Abdelkader, Faten; ÇAKMAK, İBRAHİM; NUR, ZEKARİYA; İncebıyık, Ece; Aktar, Ahmet; ERDOST, HATİCE; Bursa Uludağ Üniversitesi/Arıcılığın Geliştirilmesi-Uygulama ve Araştırma Merkezi.; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Zootekni Bölümü.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.; 0000-0003-4063-5521; 0000-0002-1438-221X; 0000-0002-2975-2594; AAH-2635-2021; GAF-2568-2022; KFW-7236-2024; CWI-2986-2022; CBC-1744-2022; ETW-1227-2022The effect of agrochemicals and beekeeping treatments on drones have not been widely investigated compared to workers or queens. In the present study, we investigated the chronic exposure of chemicals set (deltamethrin, acetamiprid, oxalic acid, fumagillin, and amitraz) on some sperm parameters and on the histomorphology of seminal vesicles. We also assessed the colony development and nosema load before and after the exposure. Thirty native Apis mellifera anatolica honeybee colonies with sister queens equalized with brood and total frame of bees were used for this experiment. Five colonies were used for each group. Deltamethrin, acetamiprid and fumagillin were given as oral chronic exposure at final concentrations of 25.10(-6) mg L-1, 0.01 m L-1 and 50 mg L-1 respectively in syrup solution (50/50). Colonies were exposed to oxalic acid by spraying 5 mL per frame space of 3% (w/v) of oxalic acid dihydrate. Finally, the amitraz was applied based on the manufacturer's instructions. The concentrations chosen represented the field-realistic concentrations and those used by beekeepers in the region. Results showed that deltamethrin reduced brood production. In the same group, we found a high increase in nosema load. All treatments decreased sperm count except for fumagillin but this compound increased sperm mortality and increased the percentage of sperm with defected acrosome integrity. The amitraz exhibited a high sperm mortality and high percentage of sperm with defected membrane integrity function. The sperm parameters such as the count, the motility, the acrosome integrity, the membrane function of sperm, and the histomorphology of seminal vesicles of drones exposed to oxalic acid were the most affected. Bee medications commonly used such as oxalic acid and fumagillin should be more investigated and should be considered by beekeepers and particularly queen breeders.Publication The effect of rat adipose-derived stem cells in bone tissue regeneration(Kafkas Univ, Veteriner Fakultesi Dergisi, 2022-04-05) İlhan, Tuncay; İLHAN, TUNCAY; SALCI, HAKAN; Akkoç, Cansel G. Özgüden; Çetin, Melike; Incebıyık, Ece; Canatan, Uygur; CANATAN, UYGUR; Salcı, Hakan; ERDOST, HATİCE; Erdost, Hatice; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Genel Cerrahi Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı; 0000-0003-0712-0892; JDC-5958-2023; F-2822-2017Stem cell approaches has been increasing in the conventional medicine. This study was planned to determinate the efficacy of adipose-derived stem cells (ADSCs) on the bone fracture's regeneration by histologically, morphologically and radiologically. Primarily isolated cells were cultivated and identified for the obtaining of the third passage mesenchymal stem cells in the cell culture laboratory. The right tibias (applicated ADSCs) were planned as experimental group and the left tibias were studied as control on the clinically healthy 10 rats. The middle diaphysis of the right and left tibias was transversally cut by a thin saw and then fixed by intramedullary pin. Before surgical closure of the incised area, 106 ADSCs were injected on an absorbent material, which covered the cut site of the right tibia, in experimental group. At postoperative 45th day, cranio-caudal and lateral radiographs of the tibias pointed out that there was callus formation in the caudal and lateral parts of the right tibia. After sacrification of the animals, preparations were made for histological examinations. The results indicate that there were clear differences in the mitotic activity; and also, the experimental tibias were found to be rich in blood vessel network. As a conclusion, it was found that ADSCs can have a positive potential effect on osteogenesis in bone tissue.Publication Phenotypic characterization and differentiation of mesenchymal stem cells originating from adipose tissue(TÜBİTAK, 2019-01-01) Çerçi, Ece; Erdost, Hatice; Çerçi, Ece; ERDOST, HATİCE; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.; 0000-0002-2740-4262; AAH-9216-2021; FYC-9982-2022Stem cells are known as undifferentiated cells that are capable of divisions and self-renewal over an extended period. Adipose tissue-derived mesenchymal stem cells (ADSCs) are known to differentiate into many different organ and tissue cells. The purpose of this study was to characterize adipose tissue-derived mesenchymal stem cells in detail based on the expression status of certain surface markers and differentiation stages for clinical and laboratory applications. The migrating phase and mitotic property of the cells from adipose tissue were assessed under a microscope. Cell counting was performed during the first, second, and third passages. The cells were differentiated into osteogenic, chondrogenic, and adipogenic lineages. Stem cells were characterized by immunostaining of CD90, CD105, CD11b, and CD45 surface markers. ADSC growth kinetics were determined at the first, second, and third passages. In conclusion, ADSCs were reported in detail here based on surface markers and differentiation potentials, growth curves, and population doubling times during the first, second, and third passages.