2012 Cilt 31 Sayı 2

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Simultaneous detection of six different groups of antimicrobial drugs in milk, meat, urine, and feed matrices
(Uludağ Üniversitesi, 2013-01-29) Rumbeiha, Wilson K.; Ensley, Steve; Schrunk, Dwayne E.; Oruç, Hasan H.; Uludağ Üniversitesi/Veteriner Fakültesi/Farmakoloji ve Toksikoloji Bölümü.
Antimicrobials are widely used for animal health in food-producing animals. Therefore, antimicrobial residues are of food safety concern. Simultaneous detection of different antimicrobial residues in the same matrice is important. Therefore, the objective of this study was to detect six different group antimicrobials in milk, meat, urine, and feed matrices under experimental conditions, and to evaluate its use for routine analysis. Biochip array-based immunoassay is currently used for simultaneous detection and quantitation of different groups of six antimicrobials in milk, urine, meat, honey, and feed. Results showed that of the six target drugs, norfloxacin, ceftiofur, florfenicol, streptomycin, tylosin and tetracycline could be detected in milk, meat, urine, and in feed matrices. In conclusion, this assay can effectively detect all target antibacterials from different groups in milk, meat, urine, and feed matrices, and this assay can be used for routine detection of these antibacterial residues in the stated matrices.