2015 Cilt 9 Sayı 26

Permanent URI for this collectionhttps://hdl.handle.net/11452/31801

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    Regeneration of two sweet cherry cultivars cvs. "bing" and "dovomras" in vitro
    (Uludağ Üniversitesi, 2015-10-08) Zamanipour, Mahbube; Tehranifar, Ali; Moghadam, Ebrahim Ganji; Abedi, Bahram
    In this article we will present the main aspects regarding the behavior of two Sweet cherry cultivars in the process of meristem culture in vitro. The effect of three growth media including WPM, MS and QL and three different hormone combinations contains BAP (0, 1 and 2 mgL-1 ), 0.5 GA3 and 0.1 IBA were investigated on the multiplication of two Sweet cherry cultivars "Bing" and "Dovomras". Cultures were maintained at 26°C under a 16 hr-light/8 hr-dark with a light intensity of 2000-3000 lux from white fluorescent light. After six weeks, the number and length of new shoots, plantlets quality and leaf number were recorded. This experiment was arranged in a completely randomized designed. The result showed that there were significant differences between cultivars for the number and the length of shoots. Also there was negative relationship between shoot number and length. The highest average new number (5.66) of shoot multiplication and Plantlets quality was obtained in WPM supplemented with 2 mgL-1 BAP in "Bing" cultivar and the highest average length of shoots (2.23 cm) and leaf number induced when was cultured on MS media obtained with 1 mgL-1 BAP in "Dovomras" cultivar. In general, Plantlets had better quality on the WPM medium in comparison with other media. The results showed that Sweet cherry was more stable and had a higher regeneration in micropropagation process based on morphological traits.