2015 Cilt 9 Sayı 26

Permanent URI for this collectionhttps://hdl.handle.net/11452/31801

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    Optimization of phytase production by new isolate bacillus sp. EBD 9-1 strain using statistical experimental design
    (Uludağ Üniversitesi, 2015-10-08) Murat, Dilek; Demirkan, Elif; Sevgi, Tuba; Baygın, Eren; İktisadi ve İdari Bilimler Fakültesi; Biyoloji Bölümü
    In this study, face centered central composite design (FCCCD) of response surface methodology (RSM) was applied to describe the relationship between the tested variables, pH, temperature, rpm, incubation period and phytase production by novel isolate Bacillus sp. EBD 9-1. The design was employed by selecting pH, temperature, rpm and incubation period as the model factors and to achieve maximum yield, interaction of these factors was studied by RSM. A second order quadratic model and response surface method showed that the optimum conditions for phytase production were pH, 8.0; temperature, 38.13°C; rpm, 113.64 and incubation period, 45 h. Under these conditions, phytase activity was found to be about 228 Uml-1.