2012 Cilt 6 Sayı 17

Permanent URI for this collectionhttps://hdl.handle.net/11452/16963

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Browsing by Subject "Contamination"

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    Disinfecting effects of nano silver fluids in gerbera (Gerbera jamesonii) capitulum tissue culture
    (Uludağ Üniversitesi, 2012) Fakhrfeshani, Masoud; Bagheri, Abdolreza; Sharifi, Ahmad
    Microbial contamination is one of the most important problems in plant tissue culture and various methods are employed to reduce it. Microbial contamination is an important barrier in the development of Gerbera tissue culture and micropropagation procedure even after disinfecting with normal methods. In this experiment the antifungal and antibacterial activity of nano-silver fluids was investigated in gerbera tissue culture and the effects of four different nano-silver concentration (25, 50, 100 and 200mg L-1) in four soaking time of explants (15, 30, 60 and 180 min) were compared with two control treatments that included disinfecting explants with soaking them in Sodium Hypochlorite and Sodium Hypochlorite followed by Mercury Chloride aqueous solution. The explants were cultured on dedicated MS medium to evaluate the effects of NS on viability and other apparent properties. The analysis of variance resulted that there are significant differences in contamination rate, both among treatments and between control and treatments. The 200 mg L-1 nano-silver solution had successfully controlled bacterial and fungal contamination and had no undesired effects on regeneration of plantlets. According to reports on high levels of contamination in Gerbera tissue culture and also the hazardous environmental effects of mercury chloride, so nano-silver solution can be used as a low risk fungicide and bactericide in Gerbera capitulum tissue culture.
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    Evaluation the potential of Nano silver for removal of bacterial contaminants in valerian (Valeriana officinalis L.) tissue culture
    (Uludağ Üniversitesi, 2012) Abdi, Gholamreza
    An experiment was conducted to evaluate the potential of nano silver to remove bacterial contaminants of valerian tissue culture. Treatments were using nano silver at two stages (before and after surface sterilization along with control) with three rates (30, 60 and 120 mg l-1) at three times of soaking (30, 60 and 180 min). Nodal explants were cultured on MS medium supplemented with different concentration of Kinetin (0, 2, 4 and 5 mg l-1) and 0.1 and 0.2 mg l-1 of NAA. Optimum result for plant regeneration was obtained in media containing 4 mg l-1 Kin and 0.2 mg l-1 NAA. Results showed that using 120 mg l-1 of nano silver solution after surface sterilization resulted in the highest percentage (89%) of disinfected explants. Nano silver solution did not affect the characters measured. On the basis of the data obtained in this experiment, it was concluded that nano silver had a good potential for removing of bacterial contaminants in plant tissue culture procedures. As this is the first report on application of nano silver in in vitro culture techniques, further investigations on other plant species are needed to clarify the effectiveness of nano silver for removal of bacterial contaminants in tissue culture of other crops.