2018 Cilt 37 Sayı 1

Permanent URI for this collectionhttps://hdl.handle.net/11452/14439

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    Analysis of IGf1 C472t and TG C422t polymorphisms in Turkish grey steppe and holstein crossbred bulls
    (Uludağ Üniversitesi, 2018-03-01) Ardıçlı, Sena
    The aim of the study was to determine genotypic distribution of bovine insulin-like growth factor 1 and thyroglobulin genes in Turkish Grey Steppe (Boz) and Holstein crossbred bulls. In the study, unrelated 30 Turkish Grey and 26 Holstein crossbred cattle were used. Genomic DNA was isolated from whole blood using standart phenol-chloroform extraction method. The polymerase chain reaction-restriction fragment length polymorphism technique was used for genotyping of C472T and C422T polymorphisms in insulin-like growth factor 1 and thyroglobulin genes, respectively. Hardy-Weinberg equilibrium was calculated by using PopGene software program. In addition the population indexes including heterozygosity, number of effective alleles and polymorphism information content were determined on the basis of allelic distribution. For insulin-like growth factor 1 gene, A allele frequency was higher and BB genotype was not present in both breed groups. For thyroglobulin gene, TT genotype was not present and moreover C allele was almost fixed in both Turkish Grey and Holstein crossbred bulls. In addition, extremely low minor allele frequencies in resulted in low genetic variabilities of population indexes. The present results may be useful for future studies conducted on bovine genome with respect to insulin-like growth factor 1 and thyroglobulin genes.
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    Determination biochemical differences and rapid ıdentification of yersinia ruckeri and lactococcus garvieae ısolated from aquaculture
    (Uludağ Üniversitesi, 2018-03-19) Satıcıoğlu, İzzet Burçin; Duman, Muhammed; Altun, Soner; Uludağ Üniversitesi/Veteriner Fakültesi.
    Aquaculture has growing much more than 100% at the last decade. Rainbow trout is the most economically important fish among the aquatic species. Bacterial agent, especially, Yersinia ruckeri and Lactococcus garvieae have limited freshwater fish production all over the world. There are many reports about identification of bacterial pathogens in aquaculture but most of them should not supported with detailed biochemical features. Therefore, we aimed in this study, determination of biochemical differences and identify two bacteria in a short time by using rapid test kits. 30 isolates of each Y. ruckeri and L. garvieae that are represent different regions of Turkey were used and biochemical characteristics were determined by conventional microbiologic test and API RAPID test kits. Y. ruckeri is the more heterogenetic biochemical structure comparing to L. garvieae. All isolates were successfully identified within 5-7 hours with rapid test kits while there are some differences between each other. The comprehensive biochemical differences of Y. ruckeri and L. garvieae were shown in the present study
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    Distribution of hoof lesions and hoof health strategies on a robotic milking farm
    (Uludağ Üniversitesi, 2018-03-12) Çeçen, Göksen; İlhan, Gürkan; Orman, Abdülkadir; Uludağ Üniversitesi/Veteriner Fakültesi.
    In Turkey, the use of robotic milking technology is still very new. The scientific data showing the effects on animal health are important as well as the studies related to the economic performance of milking robots. The aim of this study was to evaluate the hoof diseases encountered on dairy cattle farms working with robotic milking system and to determine the causes and to provide the necessary precautions to prevent and control hoof health problems. In the study, 93 Holstein dairy cattle aged between 35-54 months were evaluated in a dairy farm with two robotic milking system (system capacity of 120 cows/day). The locomotion scores were recorded and the cows’ feet were examined in hoof trimming chute and claws of all cows were trimmed according to biomechanical characteristics of foot. The treatment method was determined according to the condition of the lesion in cases of hoof disease and the results of recovery were followed. In the herd, cases of claw deformation (6.4%; n=6), sole ulcer (4.3%; n=4), white line disease (3.2%; n=3), digital dermatitis (8.6%; n=8), heel lesion (6.4%; n=6), foot rot (1.0%; n=1), limax (2.1%; n=2), hoof crack (2.1%; n=2) and coroner inflammation (1.0%; n=1) were recorded. Evaluated etiologically, it has been found that mistakes in the application of bath solutions used in the single footbath at the exit of the robotic milking unit in the formation of infectious foot diseases, feeding mistakes made without regard to cow traffic in the case of non infectious foot diseases and exposure of the foot to traumas due to not paying attention to comfort in the feeding areas are effective. As a result, to prevent management malfunctions causing metabolic problems, stress and traumas in animal, the use of senseless technology should be avoided in order to ensure sustainable hoof health on robotic milking farms.
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    Efficacy of enrofloxacin against transmissible resistance gene (Qnrs and aac (6’)-Ib-Cr) - containing escherichia coli ısolates and clones
    (Uludağ Üniversitesi, 2018-02-22) Cengiz, Murat; Arslan, Erdem; Uludağ Üniversitesi/Veteriner Fakültesi.
    Aim of this study was to investigate the effects of qnrS1 and aac (6’)-Ib-cr on susceptibility, some pharmacodynamics of enrofloxacin against molecularly constructed and natural isolates of E. coli from animals. The MPCs and mutation frequencies of the drug were determined using the agar dilution method. Time-kill assays were used to determine the antimicrobial activity of the drug against E. coli strains. MPCs of enrofloxacin for qnrS1- and aac(6´)-Ib-cr-containing transformants increased from 0.128 µg/ml to 2 and 4 µg/ml. MPC:MIC ratio of enrofloxacin for transformants and E. coli isolates were 4 and 8, respectively. Mutation frequencies were markedly higher for transformants and isolates compared to control strain. Mutation frequencies diminished up to 6.2x10-18 by increasing the concentrations used in the assay. The results of this study showed that enrofloxacin is less active against E. coli strains in case of additional mutations in QRDR. The concentration-dependent bactericidal effect of enrofloxacin is only observable by the eight times the MIC and increasing concentrations did not alter the bactericidal activity. The combination therapy can be considered to fight with resistant E. coli instead of monotherapy and artificially constructed E. coli strains, which exhibit similar reactions against ENR with natural E. coli isolates, can be used to test the pharmacological efficacy of the combinations