2023 Cilt 42 Sayı 1

Permanent URI for this collectionhttps://hdl.handle.net/11452/41030

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Browsing by Department "Veteriner Fakültesi"

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    Comparison of beta-hydroxybutyrate levels in the first three weeks after calving in four healthy different breeds of cows
    (Bursa Uludağ Üniversitesi, 2023-01-18) CİHAN, HÜSEYİN; KASAP, SEVİM; ŞENTÜRK, SEZGİN; Veteriner Fakültesi; İç Hastalıkları Ana Bilim Dalı; 0000-0003-1544-6438; 0000-0001-9501-9983; 0000-0002-2465-9913
    The objective of this study was to determine the differences between the healthy Holsteın-Friesian, Jersey, Simmental, and Aberdeen Angusbreeds Beta-hydroxybutyrate (BHBA) levels at the first three weeks after calving. The blood samples were collected for analysis between 10-20 days postpartum. In this study, 80 animals were used in different regions in total. BHBA was analyzed by Ketosite® (Ketosite®, Stanbio LabrotoryInc., Boerne, TX USA). Milk fat and protein levels were immediately measured by an electronic milk analyzer (Milkoscan FT1 FOSS Industries, Denmark). Statistical analyses of the study were performed using Sigma Plot 12 (Systat Software Inc., San Lose, CA). The means of BHBA levelswere compared by One Way Anova. As in Holstein cows, blood BHBA levels increased in the early lactation period and also in other breeds. Theresults of this study presented that serum BHBA levels were significantly higher in Holsteın-Friestein cows than in Simmental and AberdeenAngus cows.
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    Comparison of glucose levels in blood samples taken from coccygeal, jugular and mammary vein of cows in lactation
    (Bursa Uludağ Üniversitesi, 2023-01-23) Akgül, Gülşah; Cihan, Hüseyin; Kasap, Sevim; Evci, Fatma Zehra; Şentürk, Sezgin; Veteriner Fakültesi; İç Hastalıkları Bölümü; 0000-0003-1544-6438; 0000-0001-9501-9983; 0000-0002-2767-7690; 0000-0002-2465-9913
    Blood glucose level is one of the most commonly used parameters in assessing the energy metabolism in dairy cows. The presented study aimed to compare the glucose concentrations in blood samples taken from the coccygeal vein, jugular vein, and mammary vein from the sameanimals at the same time. Healthy Holstein-Friesian dairy cows (n=25) in their second and third lactation were selected for the study. Theblood samples were taken from the coccygeal vein, jugular vein, and V. subcutanea abdominis (mammary vein) at the same time from eachanimal. To analyze blood glucose, Free Style Optium Blood Glucose Test Strip (Abbott, Istanbul, TURKIYE) was used. In comparison to theblood taken from both the jugular vein and coccygeal vein, glucose levels in blood samples taken from the mammary vein were significantly low (P<0.001). As a result, the determination of glucose levels in blood samples taken from the mammary veins may cause erroneous interpretations.The refore, the mammary vein for blood glucose must not be used as a sampling site.
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    Doğu Friz ırkı koyunlarda farklı tohumlama dozlarının gebelik başarısı üzerine etkisi
    (Bursa Uludağ Üniversitesi, 2023-06-12) Aktar, Ahmet; Özkan, Hasan; Bağcı, Kemal; Uzun, Ümit Can; Akkaşoğlu, Mustafa; Alişan, Selvi; Sağırkaya, Hakan; Alçay, Selim; Veteriner Fakültesi; Klinik Bilimler Bölümü; 0000-0002-2975-2594; 0000-0001-6664-1378; 0000-0003-0535-4089; 0000-0002-9231-8619; 0000-0001-6876-0821; 0000-0001-7688-2298; 0000-0001-6619-3229; 0000-0002-2472-8157
    Saha şartlarında sperma almak ve tohumlama yapmak her zaman aynı koşullarda ve temiz şartlarda yapılamamaktadır. Bu çalışmada saha şartlarında üç farklı dozda taze sperma ile intraservikal yolla suni tohumlama uygulaması sonucunda gebelik oranlarının araştırılması amaçlandı. Araştırmada Doğu Friz ırkı 60 adet koyun 3 gruba ayrıldı (n=20) ve 3 adet koç kullanıldı. Koyunlarda kızgınlıkların senkronize edilmesi amacıyla 20 mg flugeston asestat emdirilmiş süngerler 3 gruba da 12 gün süreyle yerleştirildi. Süngerlerin çıkarılacağı gün ovulasyonları senkronize etmek için tüm koyunlara 500 IU PMSG enjeksiyonu yapıldı ve 24 saat içerisinde tüm koyunların östrus gösterdiği tespit edildi. Aynı çiftlikteki aynı ırk 3 koçtan alınan spermalarla östrustaki koyunlar intraservikal yolla tohumlandı. Tohumlama dozları1.grup için 100x10⁶ spermatozoa/ml, 2.grup için 200x10⁶ spermatozoa/ml, 3.grup için 400x10⁶ spermatozoa/ml olarak belirlendi. Tohumlamaların ardından toplamda 28 adet (%47) koyunda fertilizasyon olmadığı ve tekrar kızgınlık gösterdiği, diğer 32 adet (%53) koyunun gebe kaldığı tespit edildi. Gerçekleştirilen istatistiksel analiz sonucunda 3.Grup (400x10⁶) gebelik oranı 1. Gruba (100x10⁶) göre anlamlı olarakdaha başarılı bulunmuştur (P<0.05). Sonuç olarak tohumlama dozları kıyaslandığında saha koşullarında 400x10⁶ spermatozoa/ml dozda sulandırılmış taze sperma ile tohumlamanın yüksek gebelik oranlarının elde edilmesinde etkili olduğu belirlendi.
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    Gender determination by PCR assay for the sex-determining region Y(SRY) gene amplification in linnaeus’s two-toed sloth (Choloepus Didactylus)
    (Bursa Uludağ Üniversitesi, 2023-06-15) Bozkurt, Berkay; Vatansever, Ezgi; ARDIÇLI, SENA; Veteriner Fakültesi; Genetik Ana Bilim Dalı; 0000-0003-2758-5945
    In Linnaeus’s two-toed sloths (Choloepus didactylus), there is no distinct sexual dimorphism. It is an obstacle for gender determination fromthe external genitalia, especially in newborns or young sloths. Hence, easy, rapid, and reliable genetics-based methods for gender identificationof the sloths are needed to continue captive breeding more successfully. In this study, a PCR-based technique that allows gender determinationof two-toed sloths by using a sex-determining region Y (SRY) gene marker was described. The hair samples from young (suspectgender) and adult sloths (known gender) were used in genetic analysis. Initially, genomic DNA was isolated from hair root samples using Roche high pure PCR template preparation kit. The SRY primers were specifically designed based on the NCBI and Ensembl databases, andthey were verified with the BLAST program concerning the two-toed sloth genome. PCR amplification with the SRY-specific primers wascarried out by a programmable thermal cycler device using FastStart High Fidelity PCR System, Roche dNTPack. The samples were then electrophoresedon 2% agarose gels and were visualized by a gel documentation and analysis system. A specific band in the electrophoresis patternis diagnostic for a male individual with a partial SRY region. Hence, the analysis demonstrated that the samples belonged to a male two-toedsloth. Two-toed sloth species are commonly preferred animals in zoos. Gender determination is inevitable for these animals in captivity tobe raised successfully and healthily. Molecular genetic techniques allow high efficiency in taxonomic evaluations and gender identification inspecies that do not display sexual dimorphism. The PCR assay described here may be helpful for a rapid genetic analysis that can be widelyused in gender determination for two-toed sloths.