Browsing by Author "Seyidoğlu, Nilay"
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Item The effect of saccharomyces cerevisiae and spirulina platensis on glutathione and leucocytes count in rabbits(Bursa Uludağ Üniversitesi, 2018-02-22) Seyidoğlu, Nilay; Galip, Nurten; Serdar, Zehra; Savaş, Nilgün; Bursa Uludağ Üniversitesi/Veteriner Fakültesi.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyokimya Anabilim Dalı.; 0000-0003-1714-410X; 0000-0002-0909-618XGlutathione is the important antioxidant agent that is used for body detoxification system. Because of the fact that ıt is crucial for protecting health. A feeding trial was conducted to evaluate the effect of natural additives such as live yeast culture Saccharomyces cerevisiae (SC) and microalgae Spirulina platensis (SP) on the glutathione and leukocytes counts of rabbits. Forty male New Zealand white rabbits, aged 5-6 weeks, were studied in 4 groups. Treatments were control group, SC (added 3 g/kg diet), SP (added 5% of the diet) and, SC and SP (added 3 g/kg diet and added 5% of the diet) respectively. The experiment lasted for 90 days and the blood samples were obtained by ear venipuncture on the 90th day. In conclusion, according to the results of this study, although not statistically significant, supplementing rabbit with S. cerevisiae or S. platensis had increased on glutathione values. Glutathione tend to be positively correlated with the addition of SC or SP. No significant difference in white blood cell counts was evidenced, even if lymphocyte counts tended to increase and neutrophil counts to decrease in rabbits fed SC or SC+SP. The determination of biological consequences (antioxidant potential, resistance to diseases, and improvement of nutritional status) requires further investigations.Item Effect of yeast culture on growth performance, haematological and biochemical ındices of new zealand white rabbits(Uludağ Üniversitesi, 2014-04-30) Seyidoğlu, Nilay; Galip, Nurten; Sonat, Füsun Ak; Uludağ Üniversitesi/Veteriner Fakültesi.Effect of Sacchoromyces cerevisiae live yeast culture on growth performance, haematological and biochemical indices of rabbits was studied with 6-7 weeks old New Zealand white rabbits. Thirty male rabbits were divided equally into 3 groups; I.control group (basal diet), II.group (added S. cerevisiae, 2 g/kg diet) and III.group (added S. cerevisiae, 4 g/kg diet). Blood samples were obtained via ear venipuncture on the 85th day of feeding yeast of rabbits. No significant difference was observed in blood composition and growth performance by S. cerevisiae. Leukocyte, haematocrit and haemoglobin concentration slightly increased while serum cholesterol slightly decreased by S. cerevisiae (p>0.05). However, monocyte count (p<0.05) increased in groups fed 2 g/kg and 4g/kg S. cerevisiae. Yeast culture has a positive effect on immune system. Increased monocyte value by S. cerevisiae is beneficial in improving the immunity. On the other hand, other parameters of natural and induced immune system may be expressed to determine. So, more studies would be necessary to elucidate the effects of supplementing yeast on immunity and determine the optimum dietary concentration in animals.Item The effects of different monochromatic colors on oxidant antioxidant balance in broilers(Bursa Uludağ Üniversitesi, 2020-02-13) Seyidoğlu, Nilay; Belenli, Deniz; Merhan, Oğuz; Bozukluhan, KadirPoultry management is associated with light due to positive effects of photoreceptors on animal’s growth, immunity and homeostasis. Colors influence the efficiency of poultry performance and behavior. Light-emitting diode (LED) can use for providing monochromatic light source. In this trial, two hundred Ross 308 broiler chicks were studied in four different light groups as follows; White (Control), Red (RL), green (GL) and blue (BL) during 42 trial days. At the end of the study, the serum Superoxide dismutase (SOD), Catalase (CAT), Glutathione Peroxidase (GPx) and malondialdehyde (MDA) enzyme activities were measured to evaluate the oxidant-antioxidant balance. The oxidant marker MDA was found the highest value in group RL (p:0.0001 ; 9.24±0.33). On the other hand, in group GL, all antioxidant parameters were found the highest value (p<0.05). Also, SOD and GPx values were increased in group GL (SOD: 150.61±9.14 ; GPx: 186.96±10.50) than BL (SOD: 139.29±5.19; GPx: 160.02±3.15) statistically (p<0.05). The results showed that red light may be a stressor, and on the contrary, green light may be a positive monochromatic color for growth, oxidant-antioxidant balance and homeostasis for broilers.Item Effects of Saccharomyces cerevisiae and Spirulina platensis on growth performances and biochemical parameters in rabbits(Kafkas Univ, 2014-05) Seyidoğlu, Nilay; Galip, Nurten; Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Anabilim Dalı.; AAS-2344-2020; 55921065100; 6603186991A feeding trial was conducted to evaluate the effect of live yeast culture Saccharomyces cerevisiae (SC) and Spirulina platensis (SP) on the growth performance and serum biochemical parameters in rabbits. Forty, male New Zealand white rabbits, aged 5-6 weeks, were studied in 4 groups. The groups; I. Control (basal diet), II. SC (added 3 g/kg diet), III. SP (added 5% of the diet), IV. SC and SP (added 3 g/kg SC and 5% SP of the diet), respectively. The experiment lasted for 90 days. Blood samples were obtained by ear venipuncture on the 90th day. Also final body weight, total weight gain, total feed intake and feed conversion ratio were evaluated at the each month of the 90th day trial. There were no significant differences occurred in growth performances and biochemical parameters, but serum globulin value decreased and albumin globulin ratio increased in SP and SC+SP groups (P<0.05). More studies would be necessary to elucidate the effects of supplementing spirulina on growth and determine the optimum dietary concentration in animals.Item The effects of Spirulina platensis (Arthrospira platensis) and Saccharomyces cerevisiae on the distribution and cytokine production of CD4+ and CD8+ T-lymphocytes in rabbits(Univ Austural Chile, 2017) Seyidoğlu, Nilay; Galip, Nurten; Budak, Ferah; Uzabacı, Ender; Uludağ Üniversitesi/Veteriner Fakültesi/Fizyoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/İmmünoloji Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Biyoistatistik Anabilim Dalı.; 0000-0001-7625-9148; 0000-0002-9634-0055; F-4657-2014; 6603186991; 6701913697; 55347697800RESUMEN. Los aditivos naturales se han convertido en uno de los mayores potenciadores alternativos de inmunidad actualmente. Spirulina platensis (SP) y Saccharomyces cerevisiae (SC) se han usado para mejorar el sistema inmunológico y la calidad de vida. El objetivo de este estudio fue regular el efecto inmune de la combinación de S. cerevisiae y S. platensis (A. platensis). Se analizaron 40 conejos blancos machos de Nueva Zelanda, de 5 a 6 semanas de edad, en 4 grupos: Control (dieta basal); SC (añadido 3 g / kg de dieta); SP (añadido 5% de la dieta); SC y SP (se añadieron 3 g/kg SC y 5% SP de la dieta), respectivamente. El experimento completo duró 90 días. Muestras de sangre fueron obtenidas mediante punción venosa de oído en el día 90. Los valores de linfocitos T CD4+ y CD8+ se determinaron mediante citometría de flujo y las citoquinas (IFN-γ e IL-4) se determinaron por ELISA. De acuerdo con los resultados, no hubo diferencias significativas en la expresión de citoquinas, pero el suero CD4+/CD8+ aumentó en los animales alimentados con SP y SC + SP dietas suplementadas (3 g/kg y 5% de la dieta, respectivamente). Se concluye que S. platensis (A. platensis) se puede utilizar como un potenciador immune, aunque se necesitan más estudios para aclarar los efectos de la suplementación de espirulina en la inmunidad.Item The effects of supplemental saccharomyces cerevisiae and phytase on growth performances and plasma biochemical parameters of broiler chickens(Uludağ Üniversitesi, 2015-03-20) Suzer, Bayram; Altınbaş, Burçin; Seyidoğlu, Nilay; Arıcan, İlker; Orman, Abdulkadir; Yıldız, Hüseyin; Yalçın, Murat; Uludağ Üniversitesi/Veteriner Fakültesi.Saccharomyces cerevisiae and phytase can be used as a feed supplement in combination or alone for broiler feeding. An experiment was performed to study the effect of supplemental different ratio S. cerevisiae and phytase combination on growth performances, plasma biochemical parameters and plasma enzyme activities of broiler chickens. Animal were feed ad libitum through the study. Sixty hundred 1 day-old broiler chicks were randomly selected and distributed into eight groups as control (C); phytase (P) (added 200 g Pyhase to 1000 kg of diet); S. cerevisiae (Y1) (added 0.1% S. cerevisiae of the diet); Y1+P (combination of group Y1 and P); S. cerevisiae (Y2) (added 0.2% S. cerevisiae of the diet); Y2+P (Combination of Y2 and P); Y4 (added 0.4% S. cerevisiae of the diet); Y4+P (Combination of Y4 and P) respectively. At the end of the study, blood samples were collected from 15 randomly selected broiler chickens from each group through the brachial vein on the 42nd day of the experiment. Plasma were separated and used for measurement of plasma biochemical parameters and enzyme activities. S.cerevisiae alone and combination of Phytase and S. cerevisiae increased body weight and body weight gain of broiler, especially in 6th week, except in group Y4. Although, the broiler chickens supplemented with just phytase or/and S. cerevisiae and their combination had lower plasma triglyceride, total cholesterol, LDL-cholesterol, glucose, calcium and GGT enzyme activity , they had higher HDL-cholesterol, phosphorus and ALT enzyme. Moreover, supplementation with just phytase or/and S. cerevisiae and their combination did not change the hematocrit, plasma total protein and AST enzyme levels of the broiler chickens. These results demonstrated that phytase and S. cerevisiae alone or/and their combination improved growth performance and body weight gain of the broiler chickens. So, S.cerevisiae and phytase combination may be used as an growth enhancer. However, more studies would be necessary to obtain the effects of supplementing yeast and phytase combination on growth.Publication Role of essential oils in antioxidant capacity and immunity in a rat model of mixed stress(South African Journal of Animal Sciences, 2021) Seyidoğlu, Nilay; Köşeli, Eda; Gurbanlı, Rovshan; Aydın, Cevat; Gurbanlı, Rovshan; Aydın, Cevat; Köşeli, Eda; 0000-0002-4812-4024; CRR-4475-2022; GRJ-2026-2022; EKK-5666-2022Animal wellbeing is a balance between environmental stress and nutrition that regulates homeostasis. Augmentation of animal feed with essential oils can promote homeostasis. The present study was designed to observe the biochemical, immunological, and biological effects of daily administration of a mixture of essential oils (EOM) in a stressed rat model. Forty-eight adult male Sprague Dawley rats were randomly allocated to four groups, namely a control group (C), a stressed group (S), a treated group (Tr), and a stressed group that received the treatment (TrS). The treatment was applied by adding EOM to the water (0.2 ml/l) three days per week for 28 days. Two chronic stressors (isolation and crowding) were applied to animals in groups S and TrS. Total oxidant status (TOS) increased in the S group compared with C, whereas it decreased when fed with EOM. Although TOS was the same in S and C, it increased in Tr compared with C. There was a significant increase in interleukin 4 (IL-4) in S compared with C, and EOM reversed the IL-4 level. Nevertheless, an increase was seen in the weights of the liver, intestine, brain, and testes in TrS compared with S. The increase in water intake was a result of stress, but feeding with EOM decreased water consumption gradually. This study showed that 0.2 ml/l EOM had protective effects on antioxidant status, immunity and liver function, and decreased water consumption under stress conditions.Item Tavşanlarda spirulina platensis ve canlı maya kültürü Saccharomyces Cerevisiae'nin bağışıklık sistemi ve büyüme performansı üzerine etkisi(Uludağ Üniversitesi, 2015-10-22) Seyidoğlu, Nilay; Galip, Nurten; Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü/Fizyoloji Anabilim Dalı.Günümüzde doğal katkı maddelerinden Spirulina platensis, Saccharomyces cerevisiae ve bunların içerikleri, büyüme performansı ve bağışıklığı ve böylece yaşam kalitesini arttırıcı olarak kullanılmaktadır. Bu çalışmada S. cerevisiae (SC) ve S. platensis (SP) kombinasyonunu büyüme performansı ve bağışıklığın düzenlemesi üzerine etkileri araştırılmıştır. Çalışmada 40 adet, 5-6 haftalık, erkek beyaz Yeni Zellanda tavşanı kullanılmıştır. Gruplar sırasıyla; I. Kontrol (bazal yem), II. SC (kg yeme 3 g ilave), III. SP (kg yeme %5 oranında ilave), IV. SC ve SP grubu (kg yeme 3 g SC ve %5oranında SP ilave). 90 günlük çalışma süresince, 15 günde bir tavşanlar tartılarak canlı ağırlık ve canlı ağırlık artışları belirlenmiştir. Yemliklerdeki artan yemler de tartılarak bu dönemlere ait yem tüketim değerleri ve yemden yaralanma oranları saptanmıştır. Kan örnekleri, 90. günde kulak venasından alınarak hematokrit değer, hemoglobin miktarı, alyuvar sayımı, akyuvar sayımı ve formül lökosit değerleri belirlenmiştir. Alınan kan örneklerinden serum ayrılarak CD4+, CD8+ ve CD4+/CD8+ T lenfosit değerleri flow sitometri ile sitokinler (IFN-γ and IL-4) ise ELİSA yöntemiyle belirlenmiştir. Deneme sonunda, her gruptan ortalama canlı ağırlığa yakın olan 7 tavşan seçilerek toplam 28 hayvanda hizmet alımı şeklinde et analizleri yaptırılmıştır. Sonuçlara göre büyüme performansında, hematolojik parametrelerde ve tavşan eti analizlerinde istatistiksel önemde bir farklılık saptanmamıştır. Bununla beraber, serum sitokin ekspresyonlarında herhangi bir farklılık gözlenmezken, SP ve SC+SP gruplarındaki hayvanlarda CD4+/CD8+ T lenfosit değerinin arttığı gözlenmiştir (p<0.05). Bu nedenle S. platensis'in immun arttırıcı olarak kullanımı önerilebilir. Ancak bu katkı maddelerinin farklı özellikte hayvan materyallerinde kullanımı ile ilgili daha derin ve ayrıntılı çalışmalara gereksinim vardır.Publication The preventive role of spirulina platensis (Arthrospira platensis) in immune and oxidative insults in a stress-induced rat model(Walter, 2021-06-01) Seyidoğlu, Nilay; Köşeli, Eda; Gurbanlı, Rovshan; Aydın, Cenk; Köşeli, Eda; Gurbanlı, Rovshan; AYDIN, CENK; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Fizyoloji Bölümü; 0000-0002-4812-4024; DWC-5118-2022; CRR-4475-2022; GRJ-2026-2022Introduction: There is a balance between oxidative stress, antioxidant capacity and immune response. Their roles in physiological and behavioural mechanisms are important for the maintenance of the organism's internal equilibrium. This study aimed to evaluate the antioxidant effects of the exogenous alga Spirulina platensis (Arthrospira platensis) in a stress-induced rat model, and to describe its possible mechanism of action. Material and Methods: Thirty-six adult male Sprague Dawley rats were separated into four groups: control (C), stress (S), S. platensis (Sp), and S. platensis + stress (SpS). The rats in groups Sp and SpS were fed with 1,500 mg/kg b.w./day Spirulina platensis for 28 days. All rats were exposed to prolonged light phase conditions (18 h light:6 h dark) for 14 days. The SpS and S groups were exposed to stress by being kept isolated and in a crowded environment. Blood samples were obtained by puncturing the heart on the 28th day. The effect of stress on serum corticosterone, oxidative stress markers (TOS, TAC, PON1, OSI) and immunological parameters (IL-2, IL-4, IFN-gamma) were tested. Also, the brain, heart, intestines (duodenum, ileum, and colon), kidney, liver, spleen, and stomach of the rats were weighed. Results: Serum corticosterone levels were higher in the S group than in the C group, and significantly lower in the SpS group than in the S group. Mean total antioxidant capacity were lower in the S group than in the C group, and Spirulina reversed this change. Although not significantly different, IL-2 was lower in the S group than in the C group. However, in the SpS group, IL-2 increased due to Spirulina platensis mitigating effects of stress. Conclusion: Male rats fed a diet with Spirulina platensis could experience significantly milder physiological changes during stress, although stress patterns may be different. Exogenous antioxidant supplements merit further investigation in animals and humans where the endogenous defence mechanism against stress may not be sufficient.CPublication The relationship between spirulina platensis and selected biomechanical indicators of tibiae in rats(Czech Academy Agricultural Sciences, 2020-01-01) Süzer, Bayram; Seyidoğlu, Nilay; Tüfekçi, Kenan; İnan, Sevda; SÜZER, BAYRAM; TÜFEKCİ, KENAN; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Anatomi Anabilim Dalı; Bursa Uludağ Üniversite/Mühendislik-Mimarlık Fakültesi,/Makine Mühendisliği Bölümü; 0000-0002-2687-1221; 0000-0001-5358-1396; AAG-7076-2021; GLV-3407-2022There are several dietary supplements, particularly herbal foods, that have been used in an attempt to improve bone growth. In this study, we aim at determining the effects of low- and high-doses of Spirulina platensis, a "Superfood", on the bone growth and biomechanical indicators. Thirty Wistar rats, weighing 250 g, at the age of 7-8 weeks were assigned to three groups: The Control group (basal diet), Low-dose group (LDG; 500 mg/kg) and High-dose group (HDG; 1 000 mg/kg) of S. platensis. S. platensis was given daily by oral gavage in a 45-day-trial. At the end of the study, the right tibiae were collected and subjected to bone biomechanical tests (bone weight, bone length, maximum load, stiffness, breaking deflection, fracture toughness, post-yield displacement and yield load). Serum samples were also analysed for the calcium and phosphorus concentrations. There were significant increases in bone weight, bone length, maximum load, breaking deflection, work to fracture, post-yield displacement and yield load (P = 0.025, P = 0.019, P = 0.030, P = 0.015, P = 0.031, P = 0.028, P = 0.049, respectively), whereas stiffness non-significantly increased. However, there were no significant differences (P > 0.05) for any variables between the LDG and the HDG. Although the serum phosphorus concentrations showed no differences among any of the groups, the serum calcium concentration increased significantly in LDG compared to Control group (P = 0.009; 7.14 +/- 0.47 and 9.45 +/- 0.67, respectively). However, no differences were observed in HDG in terms of serum calcium. In conclusion, S. platensis had positive effects on the bone growth and biomechanical bone features. Therefore, our study supports the use of S. platensis as an alternative food additive for bone growth and health in growing animals.