Browsing by Author "Sevimli, Alper"
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Item Case of systemic actinobacillosis in a dog - A case report(National Veterinary Research Institute, 2006) Sevimli, Alper; Kennerman, Engin; Salcı, Hakan; Uludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Cerrahi Anabilim Dalı.; T-4623-2019; 16031244000; 16032228500A 4-year-old male German Shepherd dog was presented with a history of compromised respiratory function, lack of appetite, and weight loss. A physical examination suggested intra-thoracic pathological lesions, and was later confirmed by radiography and Computed tomography. Intrathoracic granulomatous mass as well as lesions in other organs were found in the autopsy.. Microbiological analysis revealed Actinobacillus lignieresii as a causative agent of the disease.Item Chronic amyloid arthropathy and increased serum amyloid levels in brown layers(Natl Veterinary Research Institute, 2006) Sevimli, Alper; Uğuz, Cevdet; Alasonyalılar, Aylin; Mısırlıoğlu, Deniz; Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; 8529950700; 6507455482Serum amyloid-A (SAA) levels were investigated in chickens with experimentally induced amyloid arthropathy in comparison with healthy Counterparts. Forty-eight 5-week-old chickens were allocated into two equally numbered groups. Enterococcus faecalis was injected intraarticularly at concentrations of 10(9) cfu/ml, to induce amyloid arthropathy in one of the groups, whereas the other one was kept as a control and injected intraarticularly only with 0.9% NaCl (1 ml). All the chickens were necropsied at the 13(th) week after the injections. Joint sections were examined histopathologically and immunohistochemically. Blood samples were collected and SAA levels were determined by ELISA. Amyloid accumulation in joints was only seen in the experimental group (18/24). The SAA levels found were 154 +/- 20 ng/ml and 419 +/- 27 ng/ml in the control and experimental groups, respectively, and the differences were highly significant at (P < 0.001). In conclusion, SAA plasma concentrations are influenced by amyloid arthropathy. Consequently, SAA may be a sensitive variable to assess the physical welfare in chicks; and increases in these values can be suggestive of chronic inflammatory processes, including amyloid arthropathy.Item Effects of capsaicin on nitric oxide synthase isoforms in prepubertal rat ovary(Taylor & Francis, 2012-04) Altunbaş, Korhan; Tütüncü, Şerife; Özden, Osman; Sevimli, Alper; Zik, Berrin; Peker, Sabire; Akkoç, Cansel Güzin Özgüden; Uludağ Üniversitesi/Veteriner Fakültesi/Histoloji-Embriyoloji Anabilim Dalı.; F-2822-2017; AAH-9810-2021; 6507763192; 55109615900; 36138936300Nitric oxide (NO) has emerged as an important intra-ovarian regulatory factor. We investigated effects of low dose capsaicin (CAP) treatment on the different NOS isoforms in prepubertal rat ovaries. Fifteen 21-day-old female Sprague-Dawley rats were divided randomly into three groups. The first group received no treatment, the second group received 0.5 mg/kg/day CAP dissolved in the vehicle, and the third group was treated with the vehicle only. The animals were euthanized by ether inhalation after 15 days and their ovaries were excised. Ovaries were fixed in 10% neutral buffered formalin and embedded in paraffin. Sections were processed for standard immunohistochemistry using the labeled streptavidin-biotin technique for expression of nNOS, eNOS and iNOS. We demonstrated that CAP induced expression of NOS isotypes including eNOS, iNOS and nNOS in prepubertal rat ovaries. CAP may lead to release of NO either directly from nerves or indirectly by evoking release from other cells via the action of neuropeptides that are released from afferent terminals and are involved in regulating female reproductive function.Item The effects of vitamin A, pentoxyfylline and methylprednisolone on experimentally induced amyloid arthropathy in brown layer chicks(Taylor & Francis, 2005-04) Sevimli, Alper; Uğuz, Cevdet; Deniz, Mısırlıoğlu; Polat, Ümit; Yalçın, Murat; Akkoç, A. N.; Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Psikoloji Ana Bilim Dalı.The effects of vitamin A, pentoxyfylline and methylprednisolone on experimentally induced amyloid arthropathy were investigated. In this study, 175 1-day-old brown layer chicks were used. Throughout the study Group II ( vitamin A) received high doses of vitamin A ( 75 000 IU/kg), whereas Group I ( negative control), Group III ( positive control), Group IV ( pentoxyfylline) and Group V ( methylprednisolone) received normal levels of vitamin A in the diet. At the fifth week, the experimental Groups II, III, IV and V were injected with Freund's adjuvant intra-articularly to induce amyloid arthropathy. Group IV received pentoxyfylline and Group V received methylprednisolone ( 10 mg/kg, intramuscularly) once. Joint and blood samples were examined 13 weeks after the injections. The values in Groups I, II, III, IV and V, respectively, were as follows: amyloid arthropathy formation (%), 0, 100, 87, 76, 66; serum amyloid A (ng/ml), 1669/17, 607 +/- 40, 423 +/- 39, 342 +/- 27, 293 +/- 22; serum retinol (mu g/dl): 59.75 +/- 3.8, 42.72 +/- 3, 59.24 +/- 3.6, 102 +/- 9.1, 101.3 +/- 12.3; heterophil/lymphocyte ratio: 0.504, 0.75, 0.75, 0.087, 0.44. In conclusion, it was observed that vitamin A enhanced the development of amyloid arthropathy and there were positive associations between amyloidosis, increased levels of serum amyloid A and increased numbers of tissue infiltrating macrophages. Methylprednisolone had a more successful inhibitory effect on amyloid arthropathy than pentoxyfylline.Item Enterococcus faecalis ile enfekte edilen yumurtacı piliçlerde A vitamini'nin amiloid artropati oluşumunu artırıcı etkisi(TÜBİTAK, 2004) Sevimli, Alper; Mısırlıoğlu, Deniz; Özakın, Cüneyt; Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji Anabilim Dalı.; 0000-0001-5428-3630; AAG-8392-2021; 6507915874; 6507455482; 57200678942The enhancing effect of vitamin A on the occurrence of amyloid arthropathy in Brown layer chickens is reported. In this study, 36 female and 36 male chickens were used. The chickens were allocated into 3 groups. Group I (Vitamin A group) was fed a diet which contained high levels of Vitamin A (75,000 I.U/kg), whereas group II (positive control) and group III (negative control) were fed a diet containing normal levels of vitamin A. In the fifth week of the experiment Enterococcus faecalis (10(9) cfu/ml) was injected into the left femoro-tibial joint in groups I and II (in order to produce amyloid arthropathy) and 0.9% NaCl (0.25 ml) in group Ill. At the end of the study, the amyloidosis occurrence rates and the severity of amyloid accumulation were compared. The occurrence of amyloid arthropathy in the vitamin A, the positive control and the negative control groups was 19/20 (95%), 18/24 (75%) and 0/24 (0%), respectively, and the differences among the groups were statistically significant (P < 0,01). Severe amyloid accumulation was observed in 8/20 (42.1 %) chickens in the vitamin A group, while no severe accumulation was found in the positive control group (P < 0,05). In conclusion, these data showed that high levels of vitamin A in the diet can enhance the occurrence and the severity of amyloid arthropathy induced with E. faecalis in Brown layer chickens.Item Importance of reproductive management on fertility of mares in Turkey(Ecole Nationale Veterinaire Toulouse, 2003-05) Ülgen, Mihriban; İntaş, Kamil Seyrek; Mısırlıoğlu, Deniz; İntaş, Deniz Seyrek; Kocabıyık, Ali Levent; Sevimli, Alper; Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Mikrobiyoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Patoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Genel Cerrahi Anabilim Dalı.; 0000-0002-6307-5179; AAH-7292-2019; AAG-8117-2021; 56259664500; 6603409870; 6507455482; 6506990178; 6506905121; 6507915874This study was performed with three-hundred and fifteen mares; 110 of them were used as the examination group and 205 of them served as control. The mares in the examination group were regularly inspected and gynecologic and ultrasonographic examinations were done. In suspected cases, swab samples were taken from clitorises and / or uteries ; and biopsy samples were taken from uteri if necessary. Control group was examined only to monitor pregnancy and foaling rates. According to the clinical examination, various clinical findings were observed in 63 of 110 (57 %) mares in the examination group. Sixty three mares were examined microbiologically, 59 mares were examined cytologically and 16 mares were investigated histologically. A total of 144 microorganisms belonging to various genera and species were isolated from 56 of 63 (89 %) mares in microbiological investigation. 43 of 59 (73 %) cytologically and / or histopathologically investigated mares were found positive with regard to uterus disease and 16 (27 %) of them were found negative. Infectious cases were treated with antibiotics or antifungal drugs; chirurgical or hormonal treatment were applied in other pathological cases. The mares in the examination and control groups were compared according to their fertility parameters. The foaling rates were significantly different (74 % and 38 %) in examination and control groups, respectively.Item Investigation of serum amino acid and serum amyloid A concentrations in chickens with amyloid arthropathy(Taylor & Francis, 2012) Sevimli, Alper; Yalçın, Murat; Cangül, İ. Taci; Aydın, Sami; Uludağ Üniversitesi/Veteriner Fakültesi/Fizyoloji Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji Anabilim Dalı.; 0000-0002-5600-8162; 0000-0003-0537-2113; AAG-6956-2021; AAB-4360-2021; 57192959734; 55237918600; 7005387015Background: Increased proteolytic cleavage of serum amyloid A (SAA) may potentially contribute to the development of AA amyloid deposition Objective: To study the possible relationship between amyloid artropathy and expression of SAA and some serum amino acids. Animals and methods: Values of 15 serum amino acids and SAA were investigated in chickens with experimentally induced amyloid arthropathy. Thirty-four, 5-week-old chicks were allocated into two groups: one group was injected intra-articularly with 0.25mL complete Freund's adjuvant at the left tibio-metatarsal joint to induce amyloid arthropathy, whereas the other group served as control. All pullets were necropsied 13 weeks after injection. Collected tissue samples were examined histopathologically. Blood samples were collected and SAA concentrations were measured with enzyme-linked immunosorbent assay. High-performance liquid chromatography was used to assess the amino acid concentrations in serum. Results: Amyloid accumulation in joints occurred only in the experimental group (89%). SAA concentrations of 166 +/- 17 and 423 +/- 39 (SD) ng/mL were found in the control and experimental groups, respectively (p < 0.001). In the experimental group, an increase was observed in all examined amino acid concentrations except for citrulline. The most significant (p < 0.001) increases were noticed in serine (from 159 +/- 15 to 360 +/- 29 mu mol/L), glycine (from 151 +/- 20 to 279 +/- 16 mu mol/L), isoleucine (from 48 +/- 2 to 80 +/- 6 mu mol/L), and phenylalanine (from 49 +/- 2 to 90 +/- 3 mu mol/L). Conclusion: The results of this study suggest that there is a positive correlation between some serum amino acid values, especially serine, glycine, isoleucine, and phenylalanine, and the high concentrations of SAA in chickens with amyloid arthropathy.Item Kahverengi yumurtacı piliçlerde amiloid artropati'nin deneysel oluşturulmasında vitamin A ile immun sistemin rollerinin incelenmesi(Uludağ Üniversitesi, 2003-07-01) Sevimli, Alper; Mısırlıoğlu, Deniz; Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü/Patoloji Anabilim Dalı.Bu çalışmada amiloid artropatinin oluşturulmasında A vitamininin ve immun sistemin rolleri incelendi. Çalışmada kullanılan 175 adet günlük "Iso Brown" ırkı kahverengi yumurtacı civciv 5 gruba ayrıldı. Çalışma süresince ILgrup (A vitamini) yüksek düzeyde (75.000 1.U/kg), I. (negatif kontrol), JR. (pozitif kontrol), IV. (pentoksifilin) ve V. (metilprednizolon) gruplar ise normal düzeyde A vitamini içeren yemle beslendi. Çalışmanın 5. haftasında piliçlerin sol tibio-metatarsal eklemlerine, I. gruba %0.9'hık NaCl (0.25ml), diğer gruplara ise amiloid artropati oluşturmak amacıyla complete Freund's adjuvant (0.25ml) enjeksiyonu uygulandı. Ayrıca IV. gruba pentoksifilin, V. gruba metilprednisolon birer doz (l0mg/kg-IM) olacak şekilde enjekte edildi. Enjeksiyonu takiben 13. haftanın sonunda piliçlerin nekropsileri yapılarak tibio-metatarsal eklemleri, karaciğer, dalak, böbrek, bezli mide ve ince bağırsak, dokuları ve kan örnekleri alındı. Formaldehit tesbiti ve parafin bloklamayı takiben doku kesitleri amiloid birikiminin varlığı, şiddeti ve dokuya infiltre olan yangı hücrelerinin tip ve yoğunluğu bakımından değerlendirildi. Kan örneklerinde SAA (serum amiloid A), A vitamini ve formül lökosit değerleri ölçüldü. I., n., m, IV. ve V. gruplarda amiloid artropati oluşum oranlan (%) sırasıyla; 0, 100, 87,76, 66, SAA değerleri; (ng/ml) 166±17, 607±40, 423±39, 342±27, 293±22, serum retinol değerleri (ug/dl); 59.75±3.8, 42.72±3, 59.24±3.6, 102±9.1, 101.3± 12.3, formül lökosit değerleri (%); 0.504, 0.75, 0.75, 0.087, 0.44 olarak saptandı. Sonuç olarak, A vitamininin SAA miktarım ve dokulara makrofaj göçünü artırmak suretiyle amiloid oluşumunu artırdığı gözlendi. Sadece TNF-a (Tumor necrosis factor-a)'yı baskılayıcı özelliği olan pentoksifiline kıyasla TNF-a, IL-1 (Interleukin-1), IL-2 ve IL-6 yi baskılayan metilprednizolonun amiloid oluşumunu azaltmada daha başarılı olduğu, dolayısıyla bu sitokinlerin amiloid oluşumunda önemli rol oynadıkları sonucuna varıldı.Item The role of chicken IL-1 beta, IL-6 and TNF-alpha in the occurrence of amyloid arthropathy(Springer, 2008-10) Yağcı, Artay; Bülbül, Aziz; Oral, Arzu Yılmaztepe; Altunbas, Korhan; Sevimli, Alper; Mısırlıoğlu, Deniz; Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; 25029441600; 6507915874In this study, the roles of IL-1 beta, IL-6 and TNF-alpha in amyloid arthropathic chickens with variable amounts (severe, moderate and mild) of amyloid accumulation were investigated. The presence and the levels of cytokines were evaluated in serum and in joint tissues by using ELISA and immunohistochemistry, respectively. One hundred brown layer chicks were allocated into four groups and intra-articular injections of Freund's adjuvant were used to induce amyloid arthropathy in Groups II, III and IV. Vitamin A in group II, and methylprednisolone in Group IV were added to enhance and to reduce the severity of amyloidosis, respectively. At the end of the study, a positive correlation was observed among the incidence and severity of amyloidosis, the serum amyloid A levels and the IL-1 beta values both in the serum and tissues. Elevation in the tissue TNF-alpha levels in parallel with the severity of amyloidosis has also been noted. As a conclusion, IL-1 beta appears to play an important role in avian AA amyloidosis either alone or in combination with TNF-alpha. Further investigation is needed for understanding the role of the pro-inflammatory cytokines in avian AA amyloidosis.Item Sığırlarda digital ve interdigital dermatitis’lerde klinik tanı, sağaltım ve histopatolojik bulgular(Uludağ Üniversitesi, 2002-11-05) Görgül, Sacit O.; Kahraman, M. Müfit; Çeçen, Göksen; Akkoç, Ahmet; Gül, Nihat Y.; Sevimli, Alper; Uludağ Üniversitesi/Veteriner Fakültesi.Sığırlarda (DD) Digital dermatitis ve (IDD) İnterdigital dermatitis olgularında klinik tanı, sağaltım ve bu süreçle ilgili histopatolojik bulguların değerlendirilmesi amaçlanmıştır. Çalışma materyalini U.Ü. Araştırma, Uygulama, Sağlık ve Üretim Merkezine ve Cerrahi Kliniğine getirilen, DD ve IDD saptanılan 20 Holştayn süt sığırı oluşturmuştur. Klinik olarak topallık gösteren sığırlarda topallığın derecesinin saptanması ve nedeni oluşturan lezyonun bulunduğu ayakların mekanik temizliğinin yapılmasını takiben, DD ve IDD lezyonları; form, boyut, uni ya da bilateral oluş yönlerinden değerlendirilmiştir. Bu olgularda 0.5 cm çaplı biopsi trefini (punch biopsi) ile 0., 7., 15., 21., 28. ve 36. günlerde toplam 47 biopsi örneği alınmış ve U.Ü. Patoloji Anabilim Dalı Laboratuarında Hematoksilen Eosin ve Warthin-Starry boyama teknikleri ile histopatolojik incelemeleri yapılmıştır. Olguların tamamında antibiyoterapi, mastitis sağaltımında kullanılan mastit tüp antibiyotikler uygulanarak yapılmış ve ayaklar pansumanla korunmuştur. Olguların 11’inde hafif, 8’inde orta ve bir olguda şiddetli topallık gözlenmiştir. DD olgularının 8’inde aynı zamanda IDD saptanmıştır. Olguların 5’inde verrükoz, 4’ünde papilliform, 2’sinde hiperplazik ve 9’unda ülseratif karakterli DD belirlenmiştir. DD ve IDD olguların 14’ünde unilateral, 6’sında ise bilateral olarak saptanmıştır. Lateral ve medial tırnaklarda oluşma yönünden bir farklılık bulunmamıştır. Lezyonların boyutları ortalama olarak; 5 olguda 0. 5 x 1.0 cm (küçük), 6 olguda 1.0 x 3.0 cm (orta) ve 9 olguda 3.0 x 6.0 cm (büyük) olarak değerlendirilmiştir. Sağaltım süresi küçük lezyonlarda ortalama 10 gün, orta dereceli lezyonlarda 16 gün ve büyük dereceli lezyonlarda 22 gün olarak saptanmıştır. Histopatolojik muayene sonuçlarına göre 0. günde; tüm biopsilerde epidermal hiperplazi, hiperkeratosis, subkorneal geniş retiküler dejenerasyon alanları ve vesikül oluşumları, kıl-siğil benzeri hiperkeratolitik kitleler ve SpirochetTreponema benzeri etkenlerin varlığı saptanılmıştır. Takip eden günlerde benzer yapılar görülmüştür. Sağaltım sürecinde etkenler görülmemiştir. Dermiste perivasküler yerleşimli mononükleer hücre infiltrasyonları, kollajenden zengin genç granülasyon dokusu gözlenmiştir. Klinik olarak hastalığın tanısı, histopatolojik bulgularla desteklenmiştir. Lezyonlarda saptanılan etkenlerin, 0. günde sağaltıma başlanmasını takiben diğer histopatolojik muayenelerde görülmemesi, sağaltım sürecindeki histopatolojik kesitlerde granülasyon dokusunun gelişmesinin saptanması yapılan sağaltımın başarılı sonuç verdiğinin kanıtı olarak değerlendirilmiştir. Takip edecek çalışmalarla etken identifikasyonu yapılması ve yöremizde DD ve IDD nedeni olan spesifik bir Treponema türü olup olmadığının saptanılmasının doğru olacağı kanısına varılmıştır.Item Soluble vascular endothelial growth factor receptor-1 (sVEGFR-1) is decreased in lung cancer patients showing progression: A pilot study(Taylor & Francis, 2007) Yağcı, Artay; Sevimli, Alper; Akgöz, Semra; Yılmaztepe, Arzu O.; Ulukaya, Engin; Zık, Berrin; Yılmaz, Meryem; Erdoğan, Beril Bahadır; Koç, Melike; Tokullugil, Asuman Hatice; Karadaǧ, Mehmet; Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Göğüs Hastalıkları ve Verem Anabilim Dalı.; 0000-0002-9027-1132; 0000-0003-0463-6818; AAG-8744-2021; K-5792-2018; A-5841-2017; 8606136100; 6602927353; 6507763192; 57197051873; 8833423500; 13906271900; 6507662010; 6601970351Tumor growth and metastasis depend on angiogenesis, and the vascular endothelial growth factor (VEGF) is known to be one of the most important angiogenic factors although the knowledge about its receptors is limited. We, therefore, investigated the treatment-related changes both in the level of the soluble vascular endothelial growth factor receptor-1 ( sVEGFR-1) in the serum by ELISA and the expression of VEGFR-1 in cancer tissues by immunohistochemistry. The serum levels were studied in 38 lung cancer patients, and 55 control subjects ( 21 benign disease and 34 healthy subjects) before the chemotherapy. The treatment-related changes in serum sVEGFR-1 were evaluated in 15 patients 24 and 48 hours after treatment. In addition to serum analysis, the tissue expressions were evaluated in 32 patients before treatment. The treatment-related changes in tissue VEGFR-1 expressions were evaluated in only 12 patients 24 hours after treatment. We observed no significant difference in terms of serum sVEGFR-1 levels between malignant and nonmalignant groups (p > 0.05). There were no significant differences in the levels of sVEGFR-1 before and after treatment (p > 0.05). However, there was a significant difference between sVEGFR-1 levels in the groups (regressive, stable, progressive) classified according to the response to therapy (p = 0.043). A significant difference also was present between the expression levels of tissue VEGFR-1 in the same groups (p = 0.037). As a conclusion, we suggest that prechemotherapy sVEGFR- 1 can be helpful for prediction of long-term response to therapy, but it should be studied in larger groups to elucidate its benefit in clinics.Item Steroid receptor expression and HER-2/neu (c-erbB-2) oncoprotein in the uterus of cats with cystic endometrial hyperplasia-pyometra complex(Wiley-Blackwell Publishing, 2006) Sevimli, Alper; Mısırlıoğlu, Deniz; Nak, Deniz; Nak, Yavuz; Özyiğit, Musa Özgür; Akkoç, A. N.; Cangül, Taci; Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; 0000-0003-0537-2113; AAR-6478-2021; AAH-2873-2021; AAH-5494-2021; AAB-4360-2021; 6507455482; 9280090000; 8615464000; 55584229300; 6602486125The presence of oestrogen-alpha receptor (ER), progesterone receptor (PR), and HER-2/neu (c-erbB-2) oncoprotein in the uterine walls of 10 healthy cats and 20 subjects with cystic endometrial hyperplasia-pyometra (CEH-P) were evaluated. Lesions were graded according to the severity of cystic dilation, hyperplasia and inflammation, and were classified as normal, mild uterine hyperplasia and severe uterine hyperplasia. The ER, PR and c-erbB-2 expression in the endometrium, glandular epithelium, stromal fibroblasts and myometrial smooth muscle cells was quantified by immunohistochemistry. The ER, PR and c-erbB-2 staining patterns differed between normal uteri and uteri with CEH-P. The ER expression was tended to be higher in the endometrial surface and glandular epithelium in the severe hyperplasia group (P > 0.05) and significantly lower in the mild hyperplasia cases compared with normal endometrium (P < 0.05), whereas the PR expression in both severe and mild hyperplasia cases tended to be higher in stromal cells and glandular epithelium than those in the normal uteri. C-erbB-2 immunoreactivity was observed only in the endometrial surface and glandular epithelium of the uterine wall and immunostaining was found to be highest in cases with severe hyperplasia. As a conclusion, we suggest that c-erbB-2 oncoprotein may play a role in the pathogenesis of the CEH together with the ER and PR in cats, and that ER does not have a role in the mechanism of pyometra, whereas PR plays a role in the pathogenesis of both CEH and pyometra.Item The expressions of pAkt and PTEN in lung cancer patients 24 hours after the cisplatin-based chemotherapy: A prospective pilot study(Akad Doktorlar Yayınevi, 2011) Yağcı, Artay; Sevimli, Alper; Ulukaya, Engin; Oral, Arzu Yılmaztepe; Zık, Berrin; Arı, Ferda; Akgöz, Semra; Ursavaş, Ahmet; Bayer, Sibel; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Anabilim Dalı.; Uludağ Üniversitesi/Veterinerlik Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/TB ve Göğüs Hastalıkları Anabilim Dalı.; 0000-0002-6729-7908; 0000-0003-0463-6818; K-5792-2018; AAI-3169-2021; AAH-9810-2021; AAG-7012-2021; A-5841-2017; 6602927353; 23091316500; 6507763192; 24376085300; 14061863400; 8329319900; 57215362032Akt/PKB is a protooncogen while PTEN is a tumor suppressor gene. Their expressions are of immense importance in the development of lung cancer. However, little is known about their relations to anti-cancer treatments. Therefore, we aimed to elucidate how are these parameters affected by the treatment. Expression of phosphorylated Akt (pAkt) and PTEN have been analysed on tissues of 32 patients (stage Ill and IV) with lung cancer. In addition, the expression of these variables in 14 out of 32 patients have furtherly been analysed in terms of their response to cisplatin-based chemotherapy in vivo. Prior to and 24 h after the treatment, tumor tissues were obtained via broncoscopy and then evaluated immunohistochemically by indirect streptavidin-biotin peroxidase method. Immunoreactivity for pAkt was detected in 29 of 32 cases (91%). pAkt was observed to localize in the nucleus of positively stained cells. However, PTEN expression was found in 27 of 32 cases (84%). In contrast to the localization of pAkt that is nucleus, PTEN was however localized in the cytoplasm of positively stained cells. pAkt and PTEN expression levels of 14 post-chemotherapy patients were compared to those before chemotherapy. There was no statistically significant differences (p>0.05). Although these results do not imply any possible roles of pAkt or PTEN in the late stage lung cancer patients as a biomarker for the prediction of early response to treatment in vivo, this conclusion needs to be analyzed further at later time points in a larger cohort.