Browsing by Author "Onat, Kaan"

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Bacteriological and cytological findings of bronchoalveolar lavage fluids in foals with lower respiratory tract diseases
(Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 2015-05-01) Kasap, Sevim; Göcmen, Huban; Çatık, Serkan; Onat, Kaan; Ülgen, Mihriban; Çetin, Cengiz; Kennerman, Engin; KASAP, SEVİM; Çatık, Serkan; Onat, Kaan; ÜLGEN, MİHRİBAN; Çetin, Cengiz; KENNERMAN, ENGİN; Uludağ Üniversitesi/Veteriner Fakültesi/Dahili Tıp Bölümü; Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobioloji Bölümü; 0000-0002-2245-5781; 0000-0002-3537-354X; AAG-8117-2021; AAP-7998-2020; J-8672-2018; JLJ-9087-2023; FQU-3039-2022; J-8672-2018; FDU-5862-2022
Lower respiratory tract disease is one of the most common causes of economic loss in foals. There are several methods used for the diagnosis of this disease. Bronchoalveolar lavage is one of the most important technique for bacteriological and cytological examination. The aim of this study was to evaluate BAL samples' cytologic and bacteriologic examination of foals with lower respiratory tract disease. In the comparison of cytologic examination of BAL samples with bacterial agents and without bacterial agents statistical differences in the percentage of neutophils, macrophages (P<0.001) and lymphocytes (P<0.05) were defined. Also according to the bacteriologic examination results of the BAL samples in 20 foals following agents were mainly detected: Streptococcus equi subsp. zooepidemicus (10), Staphylococcus aereus (7), Escherichia coli (3). Totally 25 aerobic/facultative gram (+) and gram (-) bacils were isolated. In conclusion, the BAL technic is useful for cytological and bacteriological examination in horses with lower respiratory tract diseases.
Effect of Mentofin application on the clearance of Mycoplasma gallisepticum (MG) from naturally infected layer chickens' trachea
(Ankara Üniversitesi, 2015-01-01) Kahya, Serpil; Onat, Kaan; Erköse, Evren; Temelli, Seran; Eyigör, Ayşegül; Çarlı, Kamil Tayfun; KAHYA DEMİRBİLEK, SERPİL; Erköse, Evren; TEMELLİ, SERAN; Eyigör, Ayşegül; CARLI, KAMİL TAYFUN; Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Gıda Hijyeni ve Teknolojisi Anabilim Dalı.; AAI-1092-2021; AAH-2842-2021; AAI-1101-2021; JPM-2439-2023; E-3867-2010
Aim of this study was to determine if Mentofin would have any effect on Mycoplasma gallisepticum (MG) clearance from the tracheal epithelium of chickens in commercial layer flocks, which were naturally infected with MG. Results indicated that, compared to the control group, there was a significant and continuous decline in MG infection in chickens of Mentofin group determined by culture and Real-Time Polymerase Chain Reaction (MGrPCR) (P<0,05). Serology results in the control group indicated an increase in MG positivity from 25% to 40% (P>0,05), while there was no change in the Mentofin group (P>0,05). Culture results for MG positivity decreased from 85% to 5% in the Mentofin group, while this decrease was from 80% to 35% in the control group (P<0,05). There was a prominent decrease from 100% to 20% in MGrPCR positives in the Mentofin group (P<0,05) compared to a non-significant change observed from 95% to 80% in the control group (P>0,05). Results of this study indicate that Mentofin clearly had an effect on MG clearance from the tracheal epithelium, supported by detection of decline in MG infection in layers.
Investigation of contagious agalactia by bacteriological and PCR methods in sheep and goats
(Kafkas Üniversitesi, 2015-01-01) Göçmen, Hüban; Ülgen, Mihriban; Çarlı, K. Tayfun; Onat, Kaan; Kahya, Serpil; Özdemir, Ümit; Mat, Burak; Göçmen, Hüban; ÜLGEN, MİHRİBAN; CARLI, KAMİL TAYFUN; KAHYA DEMİRBİLEK, SERPİL; Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü/Veteriner-Mikrobiyoloji Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakultesi/Mikrobiyoloji Anabilim Dalı.; 0000-0002-2245-5781; 0000-0002-6307-5179; AAG-8117-2021; B-9095-2018; AAH-2842-2021; E-3867-2010
The aim of this study was diagnosis that occurrence of Contagious Agalactia by bacteriological and molecular methods in sheep and goats. A total of 339 samples from sheep and goats in Bursa, Balikesir, Canakkale and Edirne provinces were examined by bacteriological and molecular methods. The samples were 162 milk samples, 147 eye swabs, 15 joint fluids, 11 nasal swabs and 4 lung tissue. In bacteriological examination, 29 isolates were evaluated as Mycoplasma sp.. As a result of biochemical tests and growth inhibition tests, 29 (8.55%) Mycoplasma sp. were identified as 25 (7.37%) Mycoplasma agalactiae, 2 (0.58%) Mycoplasma ovipneumoniae and 2 (0.58%) Mycoplasma arginini. In molecular diagnosis, polC gene-PCR results could be detected M. agalactiae positive with 9.14% rate. As a result of this, 5 milk samples and 1 lung tissue sample were detected positive by polC-PCR while negative by bacteriological examination. The results of polC-PCR detected M. agalactiae positive with 14.19% rate of milk samples, 13.33% rate of joint fluids, 2.72% rate of eye swabs and 50% rate of lung tissue samples but nasal swabs were detected as negative. In this study, presence of Contagious Agalactia were investigated by bacteriological and molecular methods and M. agalactiae was detected as a main agent which cause disease however other Mycoplasma species which cause disease were not observed.
Isolation of first local coranavirus from cattle with winter dysentery in Turkey
(Uludağ Üniversitesi, 2014-04-30) Ertürk, Arife; Gülyaz, Veli; Gülaçtı, İrem; Özdemir, Sancak; Onat, Kaan; Akgül, Gülşah; Mecitoğlu, Zafer; Çatık, Serkan; Temizel, E. Mutlu; Şentürk, Sezgin; Şenlik, Bayram; Uludağ Üniversitesi/Veteriner Fakültesi.; Uludağ Üniversitesi/Veteriner Fakültesi/Parazitoloji Bölümü.
Winter dysentery (WD) is a very contagious disease of cattle characterized by profuse diarrhea. The precise etiology of disease is still not fully elucidated. Aim of the presented study was investigation of the causative agents of disease in a herd affected by WD and to evaluate routine haematological and biochemical parameters of cows with WD. This study was carried out on blood and fecal samples collected from 12 cows showing typical signs of disease during an outbreak of WD in Bursa, Turkey. Samples were analysed for the presence of bovine coronavirus (BCoV), Bovine viral diarrhea (BVD), Eimeria oocysts and Campylobacter spp. using ELISA, RT-PCR, flotation technique and culture. Faecal samples were inoculated in HRT cell cultures for virus isolation. Isolated viruses were identified as coronaviruses from 25% of the samples by ELISA, PCR and serum neutralisation tests. Campylobacter spp. was isolated from 33.3% cows with dysentery. BVD and Eimeria oocysts were not detected in any of the samples. Results of the presented study indicates that BcoV along with Compylobacter spp. may be the primary agent of WD in cows. However negative results for BCoV and Campylobacter spp of 58 % of feacal samples indicates that etiology of the disease is still not fully elucidated and pathogens other than BCoV and Campylobacter could also be involved in pathogenesis of the disease