Browsing by Author "Nur, Zekariya"

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Comparison of fluorgestone and medroxyprogesterone intravaginal sponges for oestrus synchronization in Saanen does during the transition period
(South African Journal of Animal Sciences, 2004) Sönmez, Cumhur; Doğan, İbrahim; Nur, Zekariya; Günay, Ülgen; Soylu, Mustafa Kemal; Uludağ Üniversitesi/Veteriner Fakültesi/Dölerme Ve Suni̇ Tohumlama Anabi̇li̇m Dalı.; 0000-0003-1976-1814; 0000-0002-1438-221X; R-8366-2018; 36762299000; 6508060684; 6603885276; 7003293300
The efficiency of medroxyprogesterone acetate (MAP) and fluorogestone acetate (FGA) sponges for synchronizing oestrus in lactating Saanen goats was investigated during the transition from non-breeding to natural breeding season. Does were treated for 11 days with 60 mg MAP (n=19) or 40 mg FGA (n=24) sponges. All does received intramuscular injections of 750 IU pregnant mare serum gonadotrophin (PMSG) and 125 μg cloprostenol (PGF2α) 48 h prior to sponge removal. Cervical artificial insemination (AI) with fresh diluted semen was performed at a fixed time (36 and 48 h) followed progestagen withdrawal. The two progestagen treatments showed no significant difference in oestrous response (100% both for MAP and FGA groups), time to the onset (15.8±09 and 15.0±06 h for the MAP and FGA groups, respectively), duration (30.5±1.9 and 34.0±1.4 h for MAP and FGA, respectively) and cessation (42.32±1.6 and 43.25±1.3 h for MAP and FGA, respectively) of the induced oestrus period. No significant difference was observed with respect to pregnancy rates determined at the 53rd day after AI (52.6 and 50.0% for MAP and FGA, respectively). These dates indicate that the use of MAP and FGA intravaginal progestagen treatments are equally efficient in synchronizing oestrus in lactating goats during the transition from the non-breeding to the natural breeding season.
Concentration of trace elements in semen and relation to semen characteristics in Arabian stallions
(Indian Veterinary Journal, 2008-11-01) Doğan, İbrahim; Oruç, Hasan Hüseyin; Nur, Zekariya; Başkaya, Ruhtan; DOĞAN, İBRAHİM; ORUÇ, HASAN HÜSEYİN; NUR, ZEKARİYA; Başkaya, Ruhtan; Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Bölümü; 0000-0003-1976-1814; 0000-0002-1438-221X; AAI-2212-2021; R-8366-2018; AAH-2635-2021; CDX-4593-2022
Environmental pollutants may influence male reproductive function. Heavy metals such as lead and cadmium are abundant environmental toxicants. The purpose of this study was to determine copper, iron, lead, zinc and cadmium concentration in the semen of Arabian stallions during breeding season, and to find correlation of these elements with conventional semen parameters.
Correlations between seminal plasma enzyme activities and semen parameters in seminal fluid of Arabian horses
(Shiraz, 2009) Doğan, İbrahim; Polat, Ümit; Nur, Zekariya; Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Biyokimya Anabilim Dalı.; 0000-0003-1976-1814; 0000-0002-1438-221X; R-8366-2018; AAH-2635-2021; 36762299000; 56235316900; 6508060684
The objective of this study was to investigate aspartate-amino-transferase (AST), gamma-glutamyl-transferase (GGT), alkaline phosphatase (ALP), lactate-dehydrogenase (LDH) and acid phosphatase (AcP) activities and semen parameters (volume, pH, concentration, total sperm number (TSN), progressive motility, dead sperm, total morphological defect (TMD) and hypo-osmotic swelling test (HOST)) in seminal plasma of Arabian horses. Furthermore, correlations between enzyme activities and semen parameters were examined. The study was performed using seven healthy Arabian stallions of proven fertility, between I I and 17 years of age, from the Karacabey Stud Farm in Bursa, Turkey. Overall, 21 semen samples were collected from stallions during the breeding season from March to May. A significant negative correlation was observed between semen volume and concentration of TMD, AST, ALP (P<0.05) and LDH (P<0.01). pH showed a significant correlation with live:dead ratio, GGT activity (P<0.05) and progressive motility (P<0.01). All semen concentrations correlated significantly with TSN, TMD, ALP, AcP (P<0.01). Furthermore, significant correlations were found between live:dead ratio and TSN, HOST (P<0.05); TSN and ALP, AcP (P<0.01); progressive motility and HOST (P<0.01), GGT (P<0.001); AST and ALP, LDH, AcP (P<0.001); GGT and LDH (P<0.05); ALP and LDH, AcP (P<0.01) and LDH and AcP (P<0.001). No significant correlation was found between enzyme activities in stallion seminal plasma and semen parameters in different months, except for pH and HOST.
Different estrus induction protocols and fixed time artificial insemination during the anoestrous period in non-lactating Kivircik ewes
(Hellenic Veterinary Medical Soc, 2018-01) Doğan, İbrahim; Nur, Zekariya; Kılınç, Bahadır; Uludağ Üniversitesi/Veteriner Fakültesi/Döllenme ve Suni Tohumlama Anabilim Dalı.; Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü.; 0000-0003-1976-1814; 0000-0002-1438-221X; R-8366-2018; AAH-2635-2021; FCY-2087-2022; 36762299000; 6508060684; 57350350400
The efficiency of medroxyprogesterone acetate (MAP) sponges or norgestomet ear implants (half or entire) for synchronizing and inducing the estrous cycle in non-lactating Kivircik ewes was investigated during the natural non-breeding period. Ewes were treated for 11 days either with 60 mg MAP sponges (group 1, n=27) or with 1.5 mg norgestomet (group 2, n=25) or with 3 mg norgestomet (group 3, n=27) ear implants. In addition, each ewe received an intramuscular injection of 500 IU of equine chorionic gonadotropin (eCG) and 125 mu g cloprostenol (PGF2 alpha), 48 h prior to progestagen removal. Double Cervical Artificial Insemination (AI) with diluted fresh semen was performed at a fixed time (36 and 48 h) following progestagen withdrawal. Mean values for estrous detection rates at the first 12 +/- 6 h and within 72 h, the time from progestagen removal to the onset of estrous, the duration of the induced estrous and pregnancy rate were found to be 46.8%, 86.1%, 26.1 +/- 7.3 h, 27.0 +/- 10.7 h and 27.8%, respectively. There were significant differences between groups 2 and 3 in the time of induced estrous onset (P<0.05). These results indicate that, each of the three protocols was equally efficient in inducing and synchronizing estrus in non-lactating Kivircik ewes during the natural non-breeding period.
Different progestagen treatment duration on estrous synchronization during the natural breeding season in non-lactating Anatolian black goats
(Brazilian Coll Animal Reproduction, 2016-08-31) Doğan, İbrahim; Nur, Zekariya; Doğan, Selda; Uludağ Üniversitesi/Veteriner Fakültesi/Döprodüksiyon ve Suni Tohumlama Anabilim Dalı.; Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü.; 0000-0002-1438-221X; 0000-0003-1976-1814; 0000-0003-2589-8585; AAH-2635-2021; R-8366-2018; I-7575-2015; 36762299000; 6508060684; 57197017096
The objective of this study was to evaluate the duration of four progestagen treatments on estrous synchronization and pregnancy rate in non-lactating Anatolian black goats during the natural breeding season. All does were divided into four groups according to progestagen treatment duration using intravaginal sponges (60 mg MAP): group 1, 13 days (n = 23), group 2, 11 days (n = 25), group 3, 9 days (n = 25) and group 4, 6 days (n = 25). In addition, 24 h before sponges removal, each doe was injected with 0.075 mg of cloprostenol (PGF2 alpha) and 500 IU eCG. The goats within the same group were naturally mated at fixed time 40 h following progestagen removal, using the same breed fertile bucks (1: 5 mating ratio). The total estrous response for the first 12 h, total estrous response within 60 h, time to onset of the induced estrus, duration of the induced estrus and pregnancy rate were 3.0, 96.9%, 26.5 +/- 0.7 h, 22.6 +/- 0.8 h and 92.0%, respectively. In terms of the onset of induced estrus there were significant differences between group 1 and groups 3 and 4 and between group 2 and group 3 (P < 0.05). The protocols used were equally efficient in synchronizing and inducing estrus in non-lactating Anatolian black does during the natural breeding season. Conclude on pregnancy rate. In addition, pregnancy rates were similar results.
Drone semen cryopreservation with protein supplemented tl-hepes based extender
(Kafkas Univ, Veteriner Fakultesi Dergisi, 2019-07-01) Alcay, Selim; ALÇAY, SELİM; Çakmak, Selvinar; Çakmak, İbrahim; ÇAKMAK, İBRAHİM; Mülkpınar, Emine; Toker, Mehmed Berk; TOKER, MEHMED BERK; Üstüner, Burcu; ÜSTÜNER, BURCU; Şen, Hasan; Nur, Zekariya; NUR, ZEKARİYA; Bursa Uludağ Üniversitesi/Veteriner Fakültesi; 0000-0002-1438-221X; AAH-2635-2021; AAH-2558-2021; AAG-7238-2021; A-2794-2014
The aim of the current study was to determine the optimum concentration of bovine serum albumin for post-thawing quality of drone sperm and this is the first study to evaluate the effect of BSA supplemented TL-Hepes based extenders for drone semen cryopreservation. Sexually mature drones were used for semen collection. Pooled semen was diluted with TL-Hepes based extender supplemented with different concentrations of BSA (1 mg/mL, 3 mg/mL, and 5 mg/mL) or without BSA (control), at a final concentration of 100x10(6) spermatozoon/mL. Motility, plasma membrane functional integrity (HOST), and defected acrosome (PSA-FITC) were evaluated in the study. At post thaw, the highest sperm motility rates were obtained in the BSA5 group (P< 0.05). Functional integrity of sperm membrane was better preserved in the BSA3 and BSA5 groups compared to the other groups. The acrosomal integrity rates were higher in BSA5 group than in control group (P< 0.05). The study shows that bovine serum albumin supplemented TL-Hepes based extenders have beneficial effect on drone semen parameters at post-thaw. The results of the study demonstrated a notable advantage of using 5 mg/mL of BSA in TL-Hepes based extender.
Effect of different thawing procedures on the quality of bull semen
(Ecole Nationale Veterinaire Toulouse, 2003-07) Ak, Kemal; Nur, Zekariya; Doğan, İbrahim; Soylu, Mustafa Kemal; Uludağ Üniversitesi/Veteriner Fakültesi/Dölerme ve Suni̇ Tohumlama Anabi̇li̇m Dalı.; 0000-0002-1438-221X; 0000-0003-1976-1814; AAH-2635-2021; R-8366-2018; 6508060684; 36762299000; 7003293300
The objective of this study was to evaluate effect of different thawing procedure to Hypo-Osmotic Swelling Test (HOST), motility and morphology of bull semen. Straw (0.25 ml) frozen semen from 4 Holstein bulls was used in this study. Post-thaw motility, morphology and hypo-osmotic swelling test (HOST) were carried out at 37degreesC for 30 sec, 50degreesC for 15 sec and 70degreesC for 5 sec. According to these protocols motility, defected acrosome, other morphological defects, total morphological defects, and swollen tail spermatozoa (HOST) values were 56.6 %, 56.7 %, 59.8 %; 3.7 %, 2.6 1.9 %; 10.7 %, 8.8 %, 5.6 %; 14.4 %, 11.5 %, 7.5 %; 37.1 %, 37.4 39.3 % respectively. Thawing protocol has affected all sperm characteristics (P < 0.05) except HOST. There was a high correlation between HOST values and motility (P < 0.01) but no correlation was observed between HOST and acrosome defects, other morphological defects and total morphological defects.
Effect of egg yolk and soybean lecithin on tris-based extender in post-Thaw Ram Semen quality and in vitro fertility
(Kafkas Üniversitesi, 2014) Üstüner, Burcu; Alçay, Selim; Nur, Zekariya; Sağırkaya, Hakan; Soylu, Mustafa Kemal; Uludağ Üniversitesi/Veterinerlik Fakültesi/Klinik Bilimler Bölümü.; 0000-0002-1438-221X; AAG-7238-2021; AAH-2635-2021; AAH-8821-2021; 18937724600; 56099810300; 6508060684; 6602400461; 8237440900
The aim of the current study was to evaluate the effect of egg yolk and different soybean lecithin concentrations on the efficiency of ram semen cryopreservation and to test the fertilizing ability of frozen-thawed ram semen. Ejaculates with a thick consistency, rapid wave motion (3-5), and >70% initial motility were pooled. Pooled semen were then divided into four groups and diluted at 1/5 (semen/extender) with 1%, 3%, 6% lecithin (L1, L3 and L6) or 20% egg yolk (EY20) using the two-step dilution method. As expected, the results of the current study showed that both motility and the rates of defective acrosomes in sperm were negatively affected by the cryopreservation procedure (P<0.001). The motility values of at 5 C and post-thawed semen in the EY20 group were significantly higher than those in the L1, L3 and L6 groups (P<0.05). There were no differences in motility rates among the lecithin groups at the dilution, cooling, equilibration or post thawing stages (P>0.05). The results of in vitro fertilization, as assessed by the rate of blastocyst formation, were more successful in the EY20 group than those noted in different lecithin groups. In conclusion, freezing ram semen with an extender containing egg yolk could yield better post-thaw sperm parameters and embryonic development compared to lecithin containing extenders.
Effect of freezing rate on acrosome and chromatin integrity in ram semen
(Ankara Üniversitesi, 2011) Nur, Zekariya; Zık, Berrin; Üstüner, Burcu; Tütüncü, Şerife; Saǧirkaya, Hakan; Özgüden, Cansel G.; Günay, Ülgen; Doǧan, İbrahim; Uludağ Üniversitesi/Veterinerlik Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı.; Uludağ Üniversitesi/Veterinerlik Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.; 0000-0003-1976-1814; AAH-2635-2021; AAG-7238-2021; AAH-8821-2021; R-8366-2018; AAH-9810-2021; 6508060684; 6507763192; 18937724600; 16551094700; 6602400461; 16550925100; 55901087200; 36762299000
The objective of the present study was to investigate the effect of different freezing rates on post-thaw sperm motility, acrosome defect, and sperm chromatin structure and apoptotic activity in ram semen. Collected semen was diluted at 1:5 (semen/extender) with Bioxel (R) (IMV technologies France) at 30 degrees C and then cooled to 5 degrees C within 1h. Cooled semen was subjected to the equilibration for 2 hours. Equilibrated semen was frozen in 0.25 ml straw at two different cooling rates (slow: 0.5 degrees C/min from 5 to -20 degrees C and fast: 5 degrees C/min from 5 to -20 degrees C). Both groups were frozen from -20 to -120 degrees C at 25 degrees C/min and stored in liquid nitrogen until use. Post-thaw (37 degrees C/30 min) sperm motility, defected acrosome (Pisum sativum agglutinin fluorescein conjugate, FITC PSA), sperm chromatin structure determined by Acridin Orange (AO) and apoptotic activity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) were evaluated. Post-thaw sperm motility, acrosome defect, AO and TUNEL for slow frozen semen were 42.8 +/- 8.8%, 31.6 +/- 12.9%, 2.9 +/- 2.4% and 2.8 +/- 1.6%, and for fast frozen semen were 36.5 +/- 9.9%, 24.7 +/- 11.1%, 3.3 +/- 2.2% and 6.3 +/- 3.4%, respectively. Post-thaw semen analyses showed that there was no significant difference between two freezing curves in terms of acrosome defect, sperm chromatin damage (AO). However, a significant difference was found for post-thaw semen motility between two groups (P<0.05). In conclusion, while the slow freezing procedure improved post-thaw sperm motility, acrosome and chromatin integrities and apoptotic index in ram spermatozoa did not show any significant difference between freezing rates.
Effect of freezing rate on goat sperm morphology and DNA integrity
(TÜBİTAK, 2015) Üstüner, Burcu; Nur, Zekariya; Alcay, Selim; Toker, Mehmet Berk; Sağırkaya, Hakan; Soylu, Mustafa Kemal; Uludağ Üniversitesi/Veteriner Fakültesi/Dölerme ve Suni Tohumlama Anabilim Dalı.; 0000-0002-1438-221X; 0000-0003-4033-9749; AAG-7238-2021; AAH-2635-2021; CBC-7350-2022; A-2794-2014; AAH-8821-2021; HKG-7604-2023; 18937724600; 6508060684; 56099810300; 56480349200; 6602400461; 7003293300
This study investigates the effect of freezing rates on the spermatological parameters of frozen and thawed Saanen goat semen. Equilibrated semen was frozen at 4 different freezing rates from +5 degrees C to -150 degrees C (G10: 10 degrees C/min, G12: 12 degrees C/min, G15: 15 degrees C/min, and G24: 24 degrees C/min) and stored in liquid nitrogen until use. Semen samples were examined for sperm motility, defective acrosomes (FITC-PSA), and DNA integrity using TUNEL after dilution with extender A at equilibration and postthaw stage. There was no significant difference among the freezing stages in terms of DNA fragmentation (P > 0.05). DNA integrity was partially affected by the freezing rate. The increase of freezing rate from 10 degrees C/min to 24 degrees C/min between +5 degrees C and -150 degrees C resulted in higher postthaw DNA damage. The study found that the freeze-thawing process is detrimental to postthawed goat semen motility (P < 0.05), acrosome integrity (P < 0.05), and DNA integrity (P > 0.05). Although the freezing rates used in the present study had no effect on postthaw sperm motility and acrosome integrity (P > 0.05), sperm DNA integrity was affected.
Effect of rainbow trout (Oncorhynchus mykiss) seminal plasma on the post-thaw quality of ram semen cryopreserved in a soybean lecithin-based or egg yolk-based extender
(Elsevier, 2015-11-15) Üstüner, Burcu; Alcay, Selim; Toker, M. Berk; Nur, Zekariya; Gökce, Elif; Sonat, Füsun Ak; Gül, Zülfiye; Duman, Muhammed; Cenize, Cafer; Uslu, Aydın; Saığrkaya, Hakan; Soylu, Mustafa Kemal; Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Fizyoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Su Hayvanları Hastalıkları Anabilim Dalı.; 0000-0001-7707-2705; 0000-0002-1438-221X; 0000-0002-8872-0074; 0000-0002-7678-3289; AAH-2635-2021; T-1697-2019; AAH-8821-2021; AAF-9939-2020; AAG-7238-2021; 18937724600; 56099810300; 56480349200; 6508060684; 56779799700; 26428428000; 56086542900; 55568071100; 57070339500; 57069822700; 6602400461; 7003293300
The aim of the current study was to evaluate the effects of different concentrations of rainbow trout seminal plasma (RTSP) (0.1%, 1% and 10%) in extenders containing either egg yolk or lecithin for use in Awassi ram semen cryopreservation. Pooled sperm were diluted in a two-step dilution method to a final concentration of 1/5 (semen/extender) in egg yolk or lecithin extender containing no RTSP, 0.1%, 1% or 10% RTSP (v/v). Semen samples were assessed for sperm motility, plasma membrane integrity [hypoosmotic swelling test (HOST) and Hoechst 33258] and defective acrosomes [FITC-conjugated Pisum sativum agglutinin (PSA-FITC)] at the following five time points: after dilution with extender A; after equilibration; and post-thaw at 0 h, 3 h and 5 h. Malondialdehyde (MDA) was examined only after thawing. Freezing and thawing procedures (dilution, equilibration and post-thaw incubation at Oh, 3 h and 5 h) negatively affected the motility (P<0.001) and acrosome integrity (P<0.001). Additionally, freezing and thawing negatively affected the plasma membrane integrity, as determined by the HOST and Hoechst 33258 (P<0.001). The extender group affected the motility (P<0.001) and the HOST results (P<0.001). Levels of MDA in the egg yolk extender with 1% RTSP group were significantly lower than in the lecithin control group (P<0.05). In conclusion, the egg yolk extender groups that were supplemented with 10% and 1% RTSP provided greater cryoprotective effects for semen survivability during 5 h incubation than the other extender groups.
Effect of seminal plasma, egg yolk, and season on the freezability of Saanen buck semen
(Natl Veterinary Research, 2009) Üstüner, Burcu; Günay, Ülgen; Nur, Zekariya; Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı.; 0000-0002-1438-221X; AAH-2635-2021; AAG-7238-2021; 18937724600; 55901087200; 6508060684
This study was to find out whether removal of seminal plasma and different egg yolk concentrations in a freezing extender reveals any effect of season on the freezability of Saanen buck semen, Semen was collected from four Saanen bucks during the breeding and non-breeding seasons. Ejaculates (more than 1 ml) with three or more mass motility, >70% initial motility, and at least >2x10(9) spermatozoa/ml were pooled. The pooled semen was firstly divided into two main groups according to removal and not removal of seminal plasma. Then each main group was divided into three subgroups according to different egg yolk concentrations. The semen was used in a Tris-based freezing extender with 6%, 12% or 18% egg yolk. 0.25 ml of straws were frozen in liquid nitrogen vapour. Motility, dead spermatozoa, defected acrosome, and other morphological defects (OMD) were evaluated in equilibrated at 5 degrees C and post-thawed semen. There were seasonal differences in the semen for motility, dead spermatozoa, and OMD. For 5 degrees C equilibrated and post-thaw semen, the presence of buck seminal plasma had a detrimental effect on motility and dead spermatozoa. On the other hand, 18% egg yolk concentration affected post-thaw semen motility (P<0.05). In conclusion, the removal of seminal plasma improved motility following the freezing-thawing procedure in the breeding and the non-breeding seasons. The results of this study showed that the breeding season and removal of seminal plasma had beneficial effects on the freezability of Saanen buck semen.
Effect of sperm pooling with seminal plasma collected in breeding or nonbreeding season on Saanen goat sperm cryosurvival
(Wiley, 2018-05) Üstüner, Burcu; Gökçe, Elif; Toker, M. Berk; Önder, Nail Tekin; Saǧırkaya, Hakan; Nur, Zekariya; Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı.; 0000-0003-4033-9749; 0000-0002-1438-221X; 0000-0001-5141-0008; AAG-7238-2021; A-2794-2014; AAH-8821-2021; AAH-2635-2021; JAC-3152-2023; 18937724600; 56779799700; 56480349200; 55670728900; 6602400461; 6508060684
The aim of this study was to determine the effects of both the removal of seminal plasma (SP) and the pre-freezing addition of seminal plasma collected during the breeding or nonbreeding season on goat sperm survival after thawing. Semen samples were pooled. One aliquot of pooled semen was used as a control group. Four aliquots were then centrifuged, and the SP was removed in Group I, pipetted but not removed in Group II, removed and then pooled for animals collected in the breeding season in Group III and removed and pooled for animals collected in the nonbreeding season in Group IV. Group samples were frozen and then were assessed for rates of sperm motility, plasma membrane functional integrity hypo-osmotic swelling test (HOST), defective acrosomes (FITC-PSA), DNA fragmentation (TUNEL) and mitochondrial membrane damage (Rhodamine 123). The results showed that pre-freezing addition of SP collected in breeding season maintained post-thaw sperm characteristics at 0hr better than SP removal group, but removing seminal plasma showed positive effects on spermatozoa, as incubation time increased to 5hr. In conclusion, the pre-freezing addition of seminal plasma did not maintain post-thaw goat sperm characteristics as successfully as in the groups with seminal plasma removed after an incubation period.
Effects of alpha-lipoic acid on ram semen cryopreservation and post-thaw life span
(Wiley, 2021-09-22) Önder, Nail Tekin; Alçay, Selim; Nur, Zekariya; Önder, Nail Tekin; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı; 0000-0001-5141-0008; JAC-3152-2023
The aim of the present study was to evaluate the effects of an alpha-lipoic acid-supplemented extender on ram semen at a post-thaw stage and after incubation (6 hr). Semen samples were collected from five Kivircik Rams. Pooled semen was diluted with an egg yolk-based extender with different concentrations of alpha-lipoic acid (0.25 mmol L-1, 0.5 mmol L-1 and 1 mmol L-1) and without alpha-lipoic acid. Motility, plasma membrane functional integrity (HOST), acrosome integrity (FITC-Pisum sativum agglutinin) and DNA integrity (TUNEL) were assessed at post-thaw and 6 hr after incubation of the frozen-thawed semen. At the post-thaw stage, 0.25 mmol L-1 alpha-lipoic acid had a positive effect on sperm motility and plasma membrane functional integrity compared to the control group (p < .05). At the post-incubation stage (6 hr), it was determined that the motility and plasma membrane functional integrity of the antioxidant groups were adversely affected compared to the control group (p < .05) and 1 mmol L-1 dose of alpha-lipoic acid had a harmful effect on DNA integrity compared to the control group (p < .05). The results of the study demonstrated that alpha-lipoic acid has positive effects at post-thaw but have harmful effects on long term to ram semen.
Effects of different cryoprotective agents on ram sperm morphology and DNAintegrity
(Elsevier Science, 2010-06) Nur, Zekariya; Zık, Berrin; Üstüner, Burcu; Sağırkaya, Hakan; Özgüden, Cansel G.; Uludağ Üniversitesi/Veterinerlik Fakültesi/Klinik Bilimler Bölümü.; Uludağ Üniversitesi/Veterinerlik Fakültesi/Veteriner Hekimliği Temel Bilimler Bölümü.; 0000-0002-1438-221X; AAH-9810-2021; AAG-7238-2021; AAH-2635-2021; AAH-8821-2021; 6508060684; 6507763192; 18937724600; 6602400461; 16550925100
This study Investigates the effects of glycerol, 1.2 propanediol, sucrose, and trehalose on post-thaw motility, morphology, and genome Integrity of Awassi ram semen. Ejaculates of thick consistency with rapid wave motion (>+++) and >70% Initial motility were pooled Sperm were diluted to a final concentration of 1/5 (semen/extender) in 0% cryoprotectant, 6% glycerol, 6% 1,2 propanediol, 62.5 mM sucrose or 62 5 mM trehalose using a two-step dilution method The equilibrated semen was frozen in 0 25-ml straws. Semen samples were examined for sperm motility, defective acrosomes (FITC-Pisum sativum agglutinin (FITC PSA)), DNA integrity (acridine orange staining (AO)) and apoptotic activity (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) and Caspase-3 activity) at four time points after dilution with extender A, after cooling to 5 C, after equilibration and post-thaw Freezing and thawing procedures (cooling at 5 C, dilution, equilibration, and thawing) had negative effects on motility (P < 0.001), acrosome integrity (P < 0.001), and DNA integrity as determined by AO (P < 0001) and TUNEL (P < 0 001) assays There were positive correlations between sperm with defective acrosomes and apoptotic (AO- and TUNEL-positive) spermatozoa In contrast, a significant negative correlation was found between sperm motility and defective acrosomes and AO- and TUNEL positivity (P < 001) The cryopreservation process acts as an apoptotic inducer in ram semen, all cryoprotectants used in the present study allowed apoptosis to some extent, with negative effects on sperm morphology and DNA integrity. The glycerol group performed better than the propanediol, sucrose, trehalose, and control groups in terms of post-thaw sperm motility but not DNA integrity.
Effects of different temperature treatments applied to deep stored bull semen on post-thaw cold shocked spermatozoa
(National Veterinary Research Institute, 2006) İleri, İrfan Kamuran; Ak, Kemal; Nur, Zekariya; Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı.; 0000-0002-1438-221X; AAH-2635-2021; 6508060684
Various treatments were applied to frozen semen during storage in containers with different nitrogen level and the effects of temperature changes due to these treatments on the resistance of spermatozoa against post-thaw cold shock were studied. Straws with frozen bull semen were placed into two identical field containers. One of these containers was the low nitrogen level container with 3 cm nitrogen level that is 2 cm above the lower end of straws and the other one was the high nitrogen level container with 17 cm nitrogen level that is 2 cm above the upper end of straws. Canister No. 1 in both containers served as control without any manipulation. Canister No. 2 were raised up to the neck of the container and kept at this level for 10 s. Canister No. 3 were taken out to such a position that their base was at the entrance of the container and kept at this level for 20 s. These manipulations were repeated 100 times for canisters No. 2 and 3, in both containers. Motility, defected acrosome, and hypo-osmotic swelling test were carried out at post-thaw (37 degrees C for 30 s) and after cold shock in a cold water bath (5-6 degrees C for 30 s). Post-thaw cold-shock was observed to cause significant damage to motility, membrane integrity, and acrosome integrity of spermatozoa. Low nitrogen level was not capable of protecting viability and morphological structures of spermatozoa. In container with low nitrogen level, raising the canister from the container and keeping it at this level for 20 s made the spermatozoa more sensitive to cold shock. In conclusion, container nitrogen level and canister manipulations had negative effects on both shocked and unshocked spermatozoa. Also the results provide evidence that not only canisters misuse but also low nitrogen level made spermatozoa more sensitive to post-thaw cold shock.
Effects of long and short-term progestagen treatments combined with PMSG on oestrus synchronization and fertility in awassi ewes during the breeding season
(Veterinarni A Farmaceuticka Univerzita Brno, 2007-07-09) Üstüner, Burcu; Günay, Ülgen; Üstüner, Hakan; Nur, Zekariya; Uludağ Üniversitesi/Veteriner Fakültesi/Hayvan Besleme Anabilim Dalı.; 0000-0002-1438-221X; 0000-0002-4341-5842; AAG-7238-2021; AAH-2635-2021; AAG-9127-2021; 18937724600; 6603885276; 6508060684; 16065222700
Synchronization of oestrus has been used to increase reproductive efficiency in most animals, including ewes. The aim of the present study was to compare the effect of the length of a progestagen treatment (12 d vs. 6 d) on synchronization efficiency (oestrus response, time to onset of oestrus and duration of oestrus) and fertility rate using fluorogestone acetate (FGA) progestagen sponge treatment with pregnant mare serum gonadotropin (PMSG) administration applied at different times of sponge removal. Ewes (n = 68) were divided into two groups; long term (LT, n = 33) and short term (ST, n = 35) groups treated with FGA progestagen sponges. At the end of intravaginal sponge treatment period the animals of each group were divided into the 3 subgroups in relation to time of PMSG (300 IU) treatment. PMSG treatment was applied 24 h before sponge removal, at sponge removal and 24 h after sponge removal for LT I and ST 1, LT2 and ST2, and LT3 and ST3, respectively. Each ewe was inseminated intra-cervically twice with skim cow milk-diluted semen (1000 x 101 motile cells/ml) 40 It and 60 h after sponge removal. Non-return rates (NRR-30) were monitored from 12 day after sponge removal to 30 day with the aid of teaser rams. Onsets of oestrus response and oestrus cessation were significantly different (P < 0.05) between the ST and LT treatment groups. Synchronization of oestrus was tighter in LT than ST group. Except for oestrus cessation, other indicators studied were not different in the ST subgroups. In the ST subgroups the oestrus cessation of the STI (88.7 +/- 15.4 h) was the shortest and differed from ST3 (120.0 +/- 14.2 h) (P < 0.05). No statistical difference was observed among all studied indicators for LT groups according to application time of PMSG (P > 0.05). The NRR-30 and lambing rate of the ST and LT after timed AI were 35.7% and 31.0% and 32.1% and 28.6%, respectively (P > 0.05).
Effects of low molecular weight cryoprotectants on the post-thaw ram sperm quality and fertilizing ability
(Elsevier, 2016-03) Alcay, Selim; Üstüner, Burcu; Nur, Zekariya; Uludağ Üniversitesi/Veteriner Fakültesi/Dölleme ve Suni Tohumlama Bölümü.; 0000-0002-1438-221X; AAH-2635-2021; AAG-7238-2021; 56099810300; 18937724600; 6508060684
The aim of the current study was to compare the cryoprotective effects of low molecular weight cryoprotectants 6% DMSO, 6% ethylene glycol, 6% 1,2 propanediol and 6% glycerol on the efficiency of ram semen cryopreservation and to test the in vitro fertilizing ability of frozen-thawed ram semen. Ejaculates with a thick consistency, rapid wave motion (3-5), and >75% initial motility were pooled. Sperm were diluted to a final concentration of 1/5 (semen/extender) in 6% glycerol, 6% DMSO, 6% ethylene glycol and 6% 1,2 propanediol using a two-step dilution method. Two hours equilibrated semen was frozen in 0.25-ml straws. As expected, the results of the current study showed that motility, HOST values and the rates of defective acrosomes in sperm were negatively affected by the cryopreservation procedure (P<0.01). In conclusion, 6% DMSO had a deleterious effect on the post thaw ram sperm parameters and embryonic development. Ethylene glycol and 1,2 propanediol successfully protected acrosomal and DNA integrities. Glycerol provided a better cryoprotection to sperm motility and plasma membrane integrity. The results of in vitro fertilization, as assessed by the rate of blastocyst formation, in glycerol group were similar to 1,2 propanediol group.
Effects of various cryoprotective agents and extender osmolality on post-thawed ram semen
(Bulletin of The Veterinary Institute in Pulawy, 2007) Ak, Kemal; Soylu, Mustafa Kemal; Nur, Zekariya; Üstüner, Burcu; Doğan, İbrahim; Sağırkaya, Hakan; Günay, Ülgen; Uludağ Üniversitesi/Veteriner Fakültesi.; 0000-0002-1438-221X; AAH-2635-2021; AAG-7238-2021; AAH-8821-2021; 7003293300; 6508060684; 18937724600; 36762299000; 6602400461; 55901087200
The influence of different extender osmolality levels and the presence of different cryoprotectants on the post-thawed semen's characteristics and post-thawed plasma membrane integrity of ram spermatozoa were studied. Ram semen was frozen with TRIS-egg yolk based extender according to two-step dilution procedures. The final concentrations of the cryoprotectants: 6% glycerol, 6% 1,2-propanediol, 62.5 mM sucrose, and 62.5 mM trehalose were studied in three different extender B osmolality levels (350, 375, and 400 mOsm). The osmolality affected significantly the post-thawed semen's motility, defected acrosomes (DA), total morphological defect (TMD), along with the sperm's plasma membrane integrity (HOST). Type of cryoprotectant exerted significant effect (P<0.001) on the post-thawed semen's motility, DA, TMD, and HOST. There was a significant interaction between the osmolality and cryoprotectant on the post-thawed motility, DA and TMD, but not on the HOST. In general, post-thawed motility, acrosomal, morphological, and membrane integrity of the semen frozen with semen extender at 400 mOsm were better than those of 350 and 375 mOsm, regardless of the type of cryoprotectant. Glycerol and 1,2-propanediol, compared to sucrose, trehalose, and control groups, did not protect the post-thawed acrosome and morphological integrity, though it did protect motility and HOST. It was concluded that glycerol based extenders with a high osmotic pressure (400 mOsm) was a better choice for ram semen freezing compared to sucrose, trehalose, and cryoprotectant free extenders. The detrimental effect of glycerol on DA and TMD could be overcome by combining glycerol with sugars and by increasing the osmotic pressure of the extender used for semen cryopreservation. Further research on the cryopreservation of ram semen should focus on the extender osmolality and combination of different cryoprotectants.
Effects of various cryoprotective agents on post-thaw drone semen quality
(Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 2015-01-01) Alçay, Selim; Üstüner, Burcu; Çakmak, İbrahim; Çakmak, Selvinar; Nur, Zekariya; ALÇAY, SELİM; ÜSTÜNER, BURCU; ÇAKMAK, İBRAHİM; Çakmak, Selvinar; NUR, ZEKARİYA; Uludağ Üniversitesi/Veteriner Fakültesi/Tohumlama Anabilim Dalı; Uludağ Üniversitesi/Veteriner Fakültesi/Arıcılık Geliştirme Uygulama ve Araştırma Merkezi; 0000-0002-1438-221X; AAH-2558-2021; AAG-7238-2021; AAH-2635-2021; JJQ-9411-2023; JLJ-9258-2023
The aim of the present study was to evaluate the effect of different cryoprotectants on post-thaw semen motility and plasma membrane integrity of drone spermatozoa. Semen was obtained from mature drones (16 days or older) harvested from four colonies. Collected semen was diluted to a final concentration of 1/5 (semen/extender) in 0% cryoprotectant (control), 6% glycerol, 6% Ethylene Glycol, 6% 1,2 propanediol or 6% DMSO using a two-step dilution method. The equilibrated semen was frozen in 0.25-ml straws. The percentage of sperm motility and swollen tails (HOST) spermatozoa were evaluated following dilution with extender A (non-cryoprotectant), equilibration and post-thaw stages. In terms of post-thaw motility and plasma membrane integrity recovery we can rank the used cryoprotectant as DMSO, Ethylene Glycol, Glycerol and 1,2 Propanediol; respectively. In conclusion, post-thaw sperm motility and plasma membrane integrity of the present study was significantly better when sperm was frozen in DMSO with respect to control, glycerol, ethylene glycol, 1,2 propanediol (P<0.05).