Browsing by Author "Mat, Burak"

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Investigation of contagious agalactia by bacteriological and PCR methods in sheep and goats
(Kafkas Üniversitesi, 2015-01-01) Göçmen, Hüban; Ülgen, Mihriban; Çarlı, K. Tayfun; Onat, Kaan; Kahya, Serpil; Özdemir, Ümit; Mat, Burak; Göçmen, Hüban; ÜLGEN, MİHRİBAN; CARLI, KAMİL TAYFUN; KAHYA DEMİRBİLEK, SERPİL; Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü/Veteriner-Mikrobiyoloji Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakultesi/Mikrobiyoloji Anabilim Dalı.; 0000-0002-2245-5781; 0000-0002-6307-5179; AAG-8117-2021; B-9095-2018; AAH-2842-2021; E-3867-2010
The aim of this study was diagnosis that occurrence of Contagious Agalactia by bacteriological and molecular methods in sheep and goats. A total of 339 samples from sheep and goats in Bursa, Balikesir, Canakkale and Edirne provinces were examined by bacteriological and molecular methods. The samples were 162 milk samples, 147 eye swabs, 15 joint fluids, 11 nasal swabs and 4 lung tissue. In bacteriological examination, 29 isolates were evaluated as Mycoplasma sp.. As a result of biochemical tests and growth inhibition tests, 29 (8.55%) Mycoplasma sp. were identified as 25 (7.37%) Mycoplasma agalactiae, 2 (0.58%) Mycoplasma ovipneumoniae and 2 (0.58%) Mycoplasma arginini. In molecular diagnosis, polC gene-PCR results could be detected M. agalactiae positive with 9.14% rate. As a result of this, 5 milk samples and 1 lung tissue sample were detected positive by polC-PCR while negative by bacteriological examination. The results of polC-PCR detected M. agalactiae positive with 14.19% rate of milk samples, 13.33% rate of joint fluids, 2.72% rate of eye swabs and 50% rate of lung tissue samples but nasal swabs were detected as negative. In this study, presence of Contagious Agalactia were investigated by bacteriological and molecular methods and M. agalactiae was detected as a main agent which cause disease however other Mycoplasma species which cause disease were not observed.
Molecular characterisation of quinolone resistance in Escherichia coli from animals in Turkey
(Wiley, 2012-08-11) Cengiz, Murat; Büyükcangaz, Esra K.; Arslan, Erdem; Mat, Burak; Şahintürk, Pınar; Sonal, Songül; Göçmen, Hüban; Şen, Ayşin; Uludağ Üniversitesi/Veteriner Fakültesi/Farmakoloji ve Toksikoloji Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.; Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü.; 0000-0002-2245-5781; 0000-0001-9018-1842; 0000-0003-4337-577X; 0000-0002-9212-8743; 0000-0002-0455-8736; AAX-7249-2021; B-9095-2018; ABE-5935-2020; ABI-4237-2020; AAL-2323-2020; AAH-1820-2021; K-3299-2019; D-1599-2019; 16635026700; 25649139400; 55236738400; 55342669100; 55342852700; 7801642676; 56414715500; 7401592869
The aim of this study were to detect the gyrA, parC and marR mutations and qnr genes (qnrA, qnrB and qnrS) in 120 strains of Escherichia coli isolated from animals. European Committee on Antimicrobial Susceptibility Testing and Clinical Laboratory Standards Institute disc diffusion and minimum inhibitory concentration (MIC) tests, respectively, were used to determine fluoroquinolone (FQ) resistance, and molecular methods were used to detect the mutations and the genes. E coli isolates with an MIC of >= 8 mg/l had mutation at Ser-80 in parC in addition to mutations at Ser-83, Asp-87 or both in gyrA. The nucleotide change was detected in marR (Ser-3 -> Asn, Ala-53 -> Glu, Gly-103 -> Ser, Tyr-137 -> His). Only four E coli isolates (3.3 per cent) contained qnrA and qnrS, and qnrB was not detected. Two E coli isolates from healthy calves also contained qnrA and qnrS. The MICs of enrofloxacin and danofloxacin for qnr-containing E coli isolates ranged from 32 mg/l to 256 mg/l. The results of this study indicated that the FQ-resistant E coli isolates presented an alteration in gyrA (Ser-83 -> Leu, Asp-87 -> Asn) and parC (Ser-80 -> Ile) with high MICs (8-256 mg/l), and there was a low prevalence of qnr genes among E coli isolated from animals.
Plasmid-mediated quinolone resistance in Escherichia coli isolated from animals
(Wiley, 2012-10) Arslan, Erdem; Cengiz, Murat; Büyükcangaz, Esra; Sonal, Songül; Şen, Ayşin; Şahintürk, Pınar; Mat, Burak; Uludağ Üniversitesi/Veteriner Fakültesi.; 0000-0002-0455-8736; 0000-0001-9018-1842; 0000-0003-4337-577X; 0000-0002-9212-8743; D-1599-2019; ABE-5935-2020; K-3299-2019; ABI-4237-2020; AAL-2323-2020; AAX-7249-2021; AAH-1820-2021
Subklinik mastitisli sığır sütlerinin mikrobiyolojik analizi ve izolatların antimikrobiyal direnç profili
(Uludağ Üniversitesi, 2013-04-19) Büyükcangaz, Esra; Mat, Burak; Alrahim Ahmed, Mohammed Khider Abd; Uludağ Üniversitesi/Veteriner Fakültesi.; Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü.
Bu çalışmada subklinik mastitisli sığır sütlerinin bakteriyolojik ve mikolojik yönden tanımlanması ve elde edilen izolatların antibiyotik duyarlılıklarının belirlenmesi hedeflendi. Bu amaçla Bursa ili ile çevre illerdeki 50 farklı sürüden 1600 adet sığıra ait 6400 meme lobu California Mastitis Testi (CMT) ile tarandı. Subklinik mastitisli olduğu tespit edilen sığırlara ait 480 adet süt örneği bakteriyolojik ve mikolojik yönden incelendi. Gram pozitif koklar API-Staphy ® (bioMérieux) ve Gram negatif basiller ise API- 20E® (bioMérieux) ticari identifikasyon panelleri ile identifiye edildi ve sonuçlar API-web sistemi ile değerlendirildi. Örneklerden izole edilen 201 adet bakterinin 48 adedi (% 23.88) Koagulaz Negatif Staphylococcus spp. (KNS) ve 153 adedi (%76.11) diğer genuslardan bakteriler olarak tanımlandı. S.xylosus ve S.epidemidis en fazla izole edilen KNS ve Streptococcus spp. ve E.coli’de KNS’ler dışında en fazla izole edilen genus olarak tanımlandılar. İncelenen süt örneklerinde ayrıca 58 adet mantar ve maya türü saptandı. Bakteriyel izolatların antimikrobiyallere karşı duyarlılığını saptamak amacıyla sekiz farklı gruptan 14 antibiyotik diski ile Kirby-Bauer Antibiyotik Duyarlılık Testi uygulandı ve sonuçlar Eucast 2012 direktiflerine göre değerlendirildi. S.hominis dışında identifiye edilen KNS’lerin tümünde çeşitli grup antibakteriyellere yüksek oranda direnç geliştiği gözlemlendi. Sonuçta iki veya daha fazla gruptaki antimikrobiyal ajana dirençli olduğu saptanan 23 adet KNS ile 26 adet diğer gruplara ait bakterinin çoklu antibiyotik direncine (MDR) sahip olduğu belirlendi.