Browsing by Author "Kurnaz, Havva"
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Publication Enzootic posthitis in post-weaning lambs: A case series(Sivar-soc Italiana Veterinari Animali Reddito, 2022-02-01) KAÇAR, YİĞİT; BATMAZ, HASAN; AKKAŞ, MEHMET EMİN; Akkaş, Mehmet Emin; Batmaz, Hasan; Kurnaz, Havva; Bursa Uludağ Üniversitesi/Veteriner Fakültesi; Bursa Uludağ Üniversitesi/Veteriner Fakültesi; AAH-4972-2021; JDD-0943-2023; HOC-6049-2023Enzootic posthitis is a disease that causes ulcerative lesions in the external genitalia of lambs, leading to negative effects on animal welfare, animal health, and profitability. The disease can be observed anywhere in the world where sheep breeding is common. Enzootic posthitis has a multifactorial aetiology but is primarily caused by rations with high protein concentration. Older lambs and adult rams during the mating term are more commonly affected, whereas the occurrence in younger lambs is a rare finding. This report describes the disease detected in 6 Merino male lambs 75-90 days old and compares their clinical and laboratory findings with six healthy lambs. Approximately 100 male lambs in a flock of 300 reportedly had varying degrees of difficulty in urinating, preputial lesions, and bloody urine. Clinical examination of the affected lambs revealed an inflamed prepuce (hot, painful, and swollen) and lesions with slight hyperemia to ulcerative changes. Preputial lesions generally consisted of circular ulcerative lesions and scabs, and the tissue was prone to bleeding when the scabs were removed. Blood and urine samples, as well as preputial swabs, were taken for laboratory analyses. Corynebacterium renale, Staphylococcus intermedius, Streptococcus uberis, and Truperella pyogenes were isolated during the microbiological examination of the preputial samples. A diagnosis of enzootic posthitis was made based upon the clinical and laboratory findings of the affected lambs. For comparison, six healthy lambs were selected and evaluated. Clinical examination and some laboratory analyses performed for all lambs revealed no statistical differences in body temperature (39.78 +/- 0.13 and 39.43 +/- 0.13 degrees C), total leukocyte count (7773.33 +/- 649.13 and 6916.66 +/- 802.25/mm(3)), and hematocrit (PCV%) (47.00% +/- 3.54 and 39.33% +/- 1.45) between the diseased and healthy lambs; however, the mean urine pH values were 8.41 and 7.66, respectively (p <0.05). After the diagnosis, ration was rearranged with addition of ammonium chloride and reduction of protein concentration. Additionally, amoxicillin (15 mg/kg, two doses 48 hours apart, IM), metamizole sodium (20 mg/kg daily for two days, IM), and vitamin C (20 mg/kg for three days, IM) were administered together with local treatment of preputial lesions by using pomade rivanol for three days. With this treatment and management practices, lesions in many animals were healed on the 7th day of treatment and completely healed on the 10th day. Here, for the first time to the best of our knowledge, we document the presence of severe and widespread enzootic posthitis in male lambs aged 75-90 days to emphasize the importance of correct ration planning during the rearing period.Publication First isolation of salmonella duisburg from quail flock(Sivar-soc Italiana Veterinari Animali Reddito, 2021-06-01) ARDIÇLI, ÖZGE; ARDIÇLI, ÖZGE; KAHYA DEMİRBİLEK, SERPİL; KAHYA DEMİRBİLEK, SERPİL; Kurnaz, Havva; Carli, Kamil Tayfun; CARLI, KAMİL TAYFUN; ÇARLI, KAMİL TAYFUN; Bursa Uludağ Üniversitesi/Veteriner Fakültesi.; 0000-0001-6077-0478; HOC-6049-2023; AAG-7421-2021The first isolation of Salmonella enterica subsp. enterica serovar Duisburg (S. Duisburg) (4,12,[27]:d:e,n,z(15)) from quails was presented in this case report. Internal organs and ileocecal parts of intestines were collected from quails at 20-day old age in the flock (total of 150 quails) located in South Marmara region of Turkey. Isolation was performed according to International Organization for Standardization Method 6579. Regarding the identification of Salmonella-suspected colonies, API 20E test strips and Phoenix 100 ID/AST system were used. Serotyping of the isolate was undertaken using the slide serum agglutination test. Minimum inhibitory concentration results showed that Salmonella isolate was susceptible to all the tested antimicrobials. Although the prominent species is chicken in poultry, quail breeding increases its importance and extensiveness. Therefore this study may be useful not only for current antibiotic practices in quail breeding but also for further studies on avian microbiology.Item Kedi ve köpek kökenli stafilokok türlerinde beta-laktam direncinin fenotipik ve genotipik karakterizasyonu(Bursa Uludağ Üniversitesi, 2022-03-01) Kurnaz, Havva; Şen, Ayşin; Bursa Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü/Mikrobiyoloji Anabilim Dalı.Bu çalışmada sağlıklı kedi ve köpeklerde Staphylococcus spp. taşıyıcılığı ile hasta kedi ve köpeklerde Staphylococcus spp. kaynaklı enfeksiyon prevalansının belirlenmesi hedeflendi. Ayrıca Staphylococcus spp.’de beta-laktam grubu antimikrobiyallere direnç profilinin fenotipik ve genotipik yöntemler ile araştırılması amaçlandı. Bu amaçla sağlıklı hayvanlardan (165 kedi ve 85 köpek) sırası ile burun, ağız, kasık ve perineal bölgeden olacak şekilde tek svab örneği, hasta hayvanlardan (56 kedi ve 95 köpek) ise hastalıkla ilişkili bölgelerden (kulak, göz, deri, ağız, burun ve yara) alınan svab örnekleri incelendi. Tüm svablar ön zenginleştirme için %6,5 sodyum klorid içeren Mueller Hinton Broth’a (MHB + %6,5 NaCI) inoküle edildi, sonrasında selektif besiyeri olarak Egg Yolk Tellurite içeren Baird Parker (BP) agar izolasyonda kullanıldı. BD Phoenix 100 otomatize cihazı kullanılarak izolatların identifikasyonu ve antimikrobiyal duyarlılık testleri yapıldı. Staphylococcus intermedius Grup (SIG) olarak belirlenen türlerin ayrımı MALDI-TOF MS ile gerçekleştirildi. Tüm Staphylococcus spp. blaZ, mecA ve mecC genleri yönünden multiplex PCR ile analiz edildi. Sağlıklı kedilerin %53,9 ve sağlıklı köpeklerin %78,8 oranında Staphylococcus spp. taşıyıcılığı bulunurken, Staphylococcus spp. prevalansı hasta kedi ve köpeklerde sırasıyla %83,9 ve %60 idi. Kedilerde izole edilen en yaygın türler S. felis, S. epidermidis ve S. aureus iken, köpeklerde S. pseudintermedius idi. İzole edilen Staphylococcus spp.’de beta-laktamaz aktivitesi fenotipik yöntemler (penisilin direnci ve nitrosefin test sonucu) ile %53,8 ve genotipik yöntemler (blaZ) ile %50,4 bulundu. Metisilin direnci fenotipik yöntemler (sefoksitin ve/veya oksasilin direnci) ile %15,8 genotipik yöntemler (mecA) ile %13,5 bulundu. İzolatlarda mecC geni tespit edilmedi. Metisilin dirençli Staphylococcus spp’nin (MRS) dağılımı S. pseudintermedius (n:18), S. epidermidis (n:8), S. aureus (n:4), S. hominis (n:2), S. cohnii subsp. cohnii (n:1), S. haemolyticus (n:1) ve S. capitis (n:1) olarak saptandı. Kedi ve köpeklerde Staphylococcus spp.’nin güncel prevalansının ve beta-laktam grubu antimikrobiyallere güncel direnç profilinin bilinmesi, hem laboratuvarlarda tanısal yaklaşımlara hem de klinisyen hekimlerin bilinçli terapotik yaklaşımlarına fayda sağlayacağı düşünülmektedir.