Browsing by Author "Khider, Mohammed"

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Detection of shiga-toxin producing e.coli (stec), enteropathogenic e.coli (epec) and enterotoxigenic e.coli (etec) from animals by m-pcr
(Bursa Uludağ Üniversitesi, 2019-11-10) Khider, Mohammed; Büyükcangaz, Esra; Sen, Aysin; Cengiz, Murat; Sonal, Songül; Bursa Uludağ Üniversitesi/Veteriner Fakültesi.; Bursa Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü.; 0000-0003-4337-577X; 0000-0002-9212-8743; 0000-0001-7601-7640; 0000-0001-9018-1842
This research investigated the presence of virulence genes encoding F41, K99, eae, Stx1, Stx2 and STa and the antimicrobial resistance of animal Escherichia coli (E. coli) isolates. Clinical isolates (n:233) were evaluated from fecal samples of cattle, sheep, goats, horses, cats and dogs collected between the years of 2010 to 2015 from Turkey. Enterohaemorrahic E.coli (EHEC) O157:H7 was detected by using cefixime tellurite sorbitol MacConkey agar (CT-SMAC) and Wellcollex E. coli (Remel®). The Kirby-Bauer disc diffusion test was performed to detect the resistance pattern of the isolates to ampicillin, Amoxycilin/clavulanic acid, enrofloxacin, ceftiofur, trimethoprim/sulfamethoxazole and tetracycline. The results showed that 40% of the ruminant isolates were identified as Shiga-toxin producing Escherichia coli (STEC). Enterotoxigenic E. coli (ETEC) was detected in samples from cattle (0.9%) and sheep (12%). Enteropathogenic E.coli (EPEC) was detected in samples from cattle (0.9%) and dogs (11.4%). EHEC O157:H7 was not detected any of the isolate. Among all E.coli isolates that carried at least one virulence gene, 8 (19%) were resistant to more than three antimicrobials, 7 (16.7%) were resistant to at least one antimicrobial and 27 (64.3%) were susceptible to all antimicrobials.
Pathogenic Escherichia coli detected in food producing ruminants in Turkey
(Parlar Scientific Puplications, 2018) Khider, Mohammed; Büyükcangaz, Esra; Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü.; Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.; 0000-0003-4337-577X; AAL-2323-2020; 57200991560; 25649139400
Clinical Escherichia coli (E.coli) isolates (n:181) recovered from feces of cattle (n:111), sheep (n:25) and goats (n:45) between 2012-2015 in Turkey were screened for the presence of the virulence genes encoding Shiga toxins (Stxl and Stx2), intimin (eae), heat-stabile enterotoxin a (STa), fimbria-related antigens F41 and K99 with multiplex PCR. The presence of E.coli 0157:H7 was also investigated using Cefxime-Tellurite Sorbitol MacConkey Agar (CT-SMAC) and WellcollexEcoli (Remel (R)). Kirby Bauer Disc Diffusion testcarried out todetect resistance pattern of the isolates for enrofloxacin, ampicillin, trimethoprim/sulfamethoxazole, ceftiofur, tetracycline and amoxycillin/clavulanic acid. The results showed that the virulence genes were detected in 9 (8.1%), 13 (52%), and 15 (33.3%) of the isolates recovered from cattle, sheep and goats, respectively. Verotoxin-producing E.coli (VTEC) represents 7.2%, 40% and 33.3% while Enterotoxigenic E.coli (ETEC) represent 0.9%, 8% and 0% of the isolates recovered from cattle, sheep and goats respectively. Enteropathogenic E.coli (EPEC) was detected only in cattle (0.9%) and none of isolate was serotyped as E.coli. 0157:H7. Among all E.coli isolates which carried virulence genes (at least one), 7 (18.9%) were multi-resistant, 6 (16.2%) were resistant for at least one antimicrobial agent and 24 (64.8%) were susceptible to all anti-microbial agents.
Postmortem findings on a group of Pica pica (Passeriformes: Corvidae)
(Ankara Üniversitesi, 2019-01-01) Girişgin, Ahmet Onur; Alasonyalılar Demirer, Aylin; Büyükcangaz, Esra; Khider, Mohammed; Birlik, Sezen; İpek, Volkan; GİRİŞGİN, AHMET ONUR; ALASONYALILAR DEMİRER, AYLİN; BÜYÜKCANGAZ, ESRA; Khider, Mohammed; BİRLİK, SEZEN; İpek, Volkan; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Parazitoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.; 0000-0002-0020-2708; 0000-0001-5874-7797; AAL-2323-2020; B-5286-2017; ABZ-7197-2022; AAB-9963-2020; EKS-2415-2022; DAZ-7569-2022
Common magpies (Corvidae: Pica pica) distribute through rural and urban areas of Turkey. Because of their distribution in urbanised regions, magpies may have some potential infectious agents which may relate to domestic animals and humans. In this study, eight common magpies brought to the animal hospital in need of medical intervention were examined for endoparasites and bacteria in a one-year period. Additionally, histopathologic examinations with related organs were carried out along with endo-parasitical, cytological and microbial examination the following necropsy. As results of the necropsies, three parasite species including two helminths and one protozoan (Passerilepis sp., Brachylaima sp., Isospora rochalimai, respectively) were identified, while Staphylococcus xylosus, S. sciuri, Escherichia coli, Klebsiella pneumonia, Salmonella spp. were isolated after microbiological examination. Histopathology revealed that subacute focal mycotic pneumonia, chronic nonpurulent granulomatous gastroenteritis, verminous enteritis, and the presence of paratyphoid nodules in liver. Both of the parasites and bacteria are the first records for Turkey's helminth/bacterial fauna in wild birds.
Tracheobronchoscopic, cytological and microbiological results of tracheal and bronchial collapse in dogs
(Polish Soc Veterinary Sciences Editorial Office, 2017-07-31) Salcı, Hakan; Çetin, Melike; Kahya, Serpil; Akkoç, Ahmet; Yılmaz, Özge; Canatan, Uygur; Çeşme, Hilal; Aslan, Vildan; Altıncı, Canan; Khider, Mohammed; Yılmaz, Zeki; Bayram, Ahmet Sami; Uludağ Üniversitesi/Veteriner Fakültesi/Cerrahi Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Göğüs Cerrahisi Anabilim Dalı.; 0000-0001-9836-0749; 0000-0003-0684-0900; 0000-0002-6227-1098; AAI-6861-2021; AAH-2842-2021; AAG-7421-2021; T-4623-2019; A-9637-2008; ABB-7580-2020; 8680329000; 56393942900; 35368679200; 55584229300; 56524019400; 57189443508; 56951171800; 57200994296; 57200984827; 57200991560; 35944810500; 8347194000
The aim of this study was to evaluate the tracheobronchoscopic, cytological and microbiological results of tracheal and bronchial collapse in dogs. In total, 8 dogs were included in the study. Clinically, tracheal palpations of the dogs were reflective of tracheal disease, and all dogs coughed on tracheal palpation. Vital parameters and hematological values of the dogs were within the normal ranges. Radiological views of the respiratory tracts and thorax were largely normal, but distinctive tracheal contours were noted in cases 3 and 6. Tracheobronchoscopy was performed under general anesthesia, and endoscopic findings (mucosal surfaces and color, prominent appearance of vessels, chondral ring abnormalities of the trachea, and the presence of bronchial and tracheal collapse) were scored. Bronchoalveolar lavage (BAL) was performed to collect samples for cytological and microbiological analysis. Five cases had tracheal collapse, and two cases had right bronchial collapse. Concurrent tracheal and right bronchial collapse were diagnosed in one case. Cytological results were not indicative of inflammation or infection, but Escherichia coli was isolated from case 2 (bronchial collapse) and case 3 (tracheal collapse). Antibiotic susceptibility results revealed that the organisms were susceptible to sulfamethoxazole/trimethoprim. Statistically, there were no significant differences between the cases in terms of total endoscopic scores. In conclusion, tracheal and/or bronchial collapse should only be diagnosed by tracheobronchoscopic examination. Cytological and microbiological analyses of the BAL fluid in these cases do not always provide valuable data for clinical practitioners.