Browsing by Author "Elston, Alison F."

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    Identification of medicinal off-flavours generated by Alicyclobacillus species in orange juice using GC-olfactometry and GC-MS
    (Wiley, 2005) Elston, Alison F.; Williams, T; Parish, Mickey E.; Rouseff, Russell L.; Göçmen, D.; Uludağ Üniversitesi/Ziraat Fakültesi/Gıda Mühendisliği Bölümü.
    Aims: The objective of this study was to identify compounds responsible for medicinal off-flavours produced by different species and strains of Alicyclobacillus in orange juice using a combination of chromatographic-coupled olfactometric techniques and gas chromatography-mass spectrometry (GC-MS). Methods and Results: Each of five Alicyclobacillus strains was inoculated into separate juice samples and incubated up to 28 days at 45degreesC. Aroma compounds in the juice were analysed by GC-olfactometry (GC-O) and confirmed using GC-MS. GC-O identified three components that were described as medicinal/antiseptic. Microbial populations were enumerated at timed intervals by spiral plating onto Alicyclobacillus agar. Within 28 days incubation, all five strains produced medicinal off-aromas from guaiacol and at least one halogenated phenol. Significance and Impact of the Study: This is the first study to evaluate individual juice aroma components produced by Alicyclobacillus species using olfactometry and to demonstrate that at least three medicinal off-flavour compounds are associated with the growth of alicyclobacilli in orange juice.