Browsing by Author "Duman, Fatma Demir"

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    Development of near-infrared region luminescent N-acetyl-L-cysteine-coated Ag2S quantum dots with differential therapeutic effect
    (Future Medicine, 2019-04) Buz, Pelin Turhan; Duman, Fatma Demir; Erkisa, Merve; Demirci, Gözde; Ulukaya, Engin; Acar, Havva Yağcı; Arı, Ferda; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji/Moleküler Biyoloji Bölümü.; 0000-0002-6729-7908; AAG-7012-2021; 24376085300
    Aim: N-acetyl-L-cysteine (NAC) is a free radical scavenger. We developed NAC-coated Ag2S (NAC-Ag2S) quantum dot (QD) as an optical imaging and therapeutic agent. Materials & methods: QDs were synthesized in water. Their optical imaging potential and toxicity were studied in vitro. Results: NAC-Ag2S QDs have strong emission, that is tunable between 748 and 840 nm, and are stable in biologically relevant media. QDs showed significant differences both in cell internalization and toxicity in vitro. QDs were quite toxic to breast and cervical cancer cells but not to lung derived cells despite the higher uptake. NAC-Ag2S reduces reactive oxygen species (ROS) but causes cell death via DNA damage and apoptosis. Conclusion: NAC-Ag2S QDs are stable and strong signal-generating theranostic agents offering selective therapeutic effects.
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    Folic acid-conjugated cationic Ag2S quantum dots for optical imaging and selective doxorubicin delivery to HeLa cells
    (Future Medicine, 2017-10) Duman, Fatma Demir; Khodadust, Rouhullah; Ulukaya, Engin; Acar, Havva Yağcı; Erkısa, Merve; Arı, Ferda; Uludağ Üniversitesi/Fen-Edebiyet Fakültesi/Biyoloji Bölümü.; 0000-0002-3127-742X; 0000-0002-6729-7908; AAM-1001-2020; AAG-7012-2021; 57126208900; 24376085300
    We aim to develop folic acid ( FA)-conjugated cationic Ag2S near-infrared quantum dots (NIRQDs) for the delivery of doxorubicin (DOX) selectively to folate receptor (FR)-positive cancer cells to achieve enhanced drug efficacy and optical tracking in the NIR region. Materials & methods: Cationic Ag2S NIRQDs were decorated with FA using a PEG bridge and loaded with DOX. In vitro studies were performed on FR-positive human cervical carcinoma cells and FR-negative A549 cells. Results: Significantly higher uptake of DOX by human cervical carcinoma cells cells and a greater therapeutic effect along with a strong intracellular optical signal were obtained with DOX-loaded FA-conjugated Ag2S NIRQDs. Conclusion: These Ag2S NIRQDs are promising theranostic nanoparticles for receptor-mediated delivery of DOX with enhanced drug efficacy combined with optical imaging.