Browsing by Author "Belenli, Deniz"
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Item The concentrations of adipokines in goat milk: Relation to plasma levels, inflammatory status, milk quality and composition(Japan Social Veterinary Science, 2016-12-02) Güzel, Saime; Yıbar, Artun; Belenli, Deniz; Çetin, İsmail; Tanrıverdi, Meltem; Uludağ Üniversitesi/Veteriner Fakültesi/Biyokimya Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Gıda Hijyeni ve Teknolojisi Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Hayvan Besleme ve Beslenme Hastalıkları Anabilim Dalı.; 0000-0003-0796-5000; AAH-4275-2021; 55460886200; 38762296900; 56743270100; 55387309300The main objectives of our study were to measure the major adipokines adiponectin, leptin and resistin in goat milk, to assess their interrelationships and to assess their relationships with the plasma and serum concentrations of total protein, cholesterol, total lipids, plasma C-reactive protein (CRP), milk somatic cell count (SCC), milk total aerobic colony and lactobacillus count, and milk components in lactating Saanen goats.The study was performed on eighteen lactating Saanen goats. Milk and blood samples were collected on days 20, 35, 50, 65 and 80 of lactation postpartum. The milk and plasma adiponectin levels on days 50, 65 and 80 postpartum were significantly higher than those on day 20.The milk and plasma leptin levels were lower on day 20 than on days 35, 50, 65 and 80. The milk concentrations of these major adipokines were positively intercorrelated. The milk and plasma concentrations of these three adipokines were also positively correlated. The plasma CRP concentrations correlated positively with milk leptin and resistin concentrations and inversely with milk adiponectin concentration. Milk adiponectin concentration was inversely related with its SCC. These data confirm that adiponectin, leptin and resistin are present in goat milk. The milk concentrations of these three adipokines were interrelated and interacted with the general inflammatory marker, CRP. The inverse relationship between milk adiponectin concentrations and its SCC suggests that variations in milk adiponectin might be involved in the udder health of lactating goats, but clinical trials are needed to support this hypothesis.Item The effects of different monochromatic colors on oxidant antioxidant balance in broilers(Bursa Uludağ Üniversitesi, 2020-02-13) Seyidoğlu, Nilay; Belenli, Deniz; Merhan, Oğuz; Bozukluhan, KadirPoultry management is associated with light due to positive effects of photoreceptors on animal’s growth, immunity and homeostasis. Colors influence the efficiency of poultry performance and behavior. Light-emitting diode (LED) can use for providing monochromatic light source. In this trial, two hundred Ross 308 broiler chicks were studied in four different light groups as follows; White (Control), Red (RL), green (GL) and blue (BL) during 42 trial days. At the end of the study, the serum Superoxide dismutase (SOD), Catalase (CAT), Glutathione Peroxidase (GPx) and malondialdehyde (MDA) enzyme activities were measured to evaluate the oxidant-antioxidant balance. The oxidant marker MDA was found the highest value in group RL (p:0.0001 ; 9.24±0.33). On the other hand, in group GL, all antioxidant parameters were found the highest value (p<0.05). Also, SOD and GPx values were increased in group GL (SOD: 150.61±9.14 ; GPx: 186.96±10.50) than BL (SOD: 139.29±5.19; GPx: 160.02±3.15) statistically (p<0.05). The results showed that red light may be a stressor, and on the contrary, green light may be a positive monochromatic color for growth, oxidant-antioxidant balance and homeostasis for broilers.Item Effects of glucosinolates and their hydrolysis products on biochemical and performance parameters in broiler chicken diets(Indian Council of Agricultural Research, 2016-03-16) Berhow, Mark Alan; Belenli, Deniz; Polat, Ümit; Orman, Abdulkadir; Yesilbağ, Derya; Berhow, Mark A.; Uludağ Üniversitesi/Veteriner Fakültesi/Biyokimya Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Hayvancılık Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Hayvan Besleme ve Beslenme Hastalıkları Anabilim Dalı.; 0000-0001-9138-4422; AAG-9134-2021; AAK-5370-2020; 56743270100; 56235316900; 24335834100; 9246817500; 7003305843Glucosinolates are important bioactive molecules and widely found in Brassicaceae species (cress, brussels sprouts, mustard, broccoli, kale etc.). Depending on the amount of these vegetables consumed, both positive and negative metabolic effects from glucosinolate metabolites may occur. The aim of this study was to investigate inexpensive animal food sources that both increase weight gain and provide enhanced performance parameters without adversely affecting the animal's health and metabolism; to evaluate dose adjustment of food containing glucosinolates in animals; and to evaluate changes in the biochemical and performance status of chickens on the glucosinolate containing diets. Day-old Ross 308 broiler line chicks (624) were divided into 1 control and 3 treatment groups. Cress seed (Lepidium sativum) was added 0.05% for the first treatment group (group 1, 10g/kg), 0.10% for the second treatment group (group 2, 20g/kg) and 0.15% for the last treatment groups (group 3, 30 g/kg) to the diet. Serum samples were evaluated for serum glucose, adiponectin, leptin, growth hormone, estradiol and cortisol levels. Feed intake, live body weight gain and feed conversion ratio were investigated for performance parameters. The results showed that dietary cress seed supplementation as feed additive (10, 20 and 30 g/kg) did not significantly improve the dietary performance, or carcass parameters of broiler chickens. Feed intake was the highest in group 2 (20g/kg), female live weight was the highest in group 2 (20 g/kg) and 3 (30 g/kg).Item Effects of glucosinolates and their hydrolysis products on energy balances and performance parameters and histological parameters in broiler chicken diets(Bursa Uludağ Üniversitesi, 2018-07-17) Belenli, Deniz; Berhow, Mark A.; Polat, Umit; Erdost, Hatice; İlhan, Tuncay; Orman, Abdülkadir; Yesilbağ, Derya; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Biyokimya Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Hayvancılık Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Hayvan Besleme ve Beslenme Hastalıkları Anabilim Dalı.Glucosinolates are important bioactive molecules and widely found in Brassicaceae species (cress, brussels sprouts, mustard, broccoli, kale etc.). Depending on the amount of these vegetables consumed, both positive and negative metabolic effects from glucosinolate metabolites may occur. The aims of this study were to; investigate inexpensive animal food sources that both increases weight gain and provides enhanced performance parameters without adversely affecting the animal’s health and metabolism; to evaluate dose adjustment of food containing glucosinolates in animals; and to evaluate changes in the biochemical and performance status of chickens on these glucosinolate containing diets. A total of 624 one-day-old Ross 308 broiler line chicks were divided into one control and three treatment groups. Cress seed (Lepidium sativum) was added 0.05% for the first treatment groups (Group 1, 10 g/kg), 0.10% for the second treatment groups (Group 2, 20 g/kg) and 0.15% for the last treatment groups (Group 3, 30 g/kg) to the diet. Serum samples were evaluated for serum glucose, adiponectin, leptin, growth hormone, estradiol and cortisol levels. Performance parameters investigated included feed intake, live body weight and feed conversion ratio. . The villus length, number of goblet cells, crypt depth were determined for histological analyses. According to histological results, villus length was significant at p < 0.05 level between control and group 1 and at p < 0.001 level with groups 2 and 3 at 21 days. The depth of the crypts belonging to the control and experimental groups was not significant between the control group and the group 1 when the statistic was evaluated on the 21st day, whereas between the control group and the group 2, p < 0.05 and p < 0.01; statistical significance was found at p < 0.001 level between group 3 and control group. The results showed that dietary glucosinolate supplementation as feed additive (10, 20 and 30 g/kg) did not significantly improve the dietary performance, or carcass parameters of broiler chickens. Feed intake was the highest in group 2 (20 g/kg), female live weight was the highest in group 2 (20 g/kg) and 3 (30 g/kg). In conclusion, the rates of the cress seed (0.05, 0.10 and 0.15%) that contain glucotropaeolin were not affected for feed additive on performance (especially live weight and live weight gain) and carcass parameters.Item Effects of supplementation with rosemary (rosmarinus officinalis l.) volatile oil on growth performance, meat mda level and selected plasma antioxidant parameters in quail diets([1])(Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 2016-10-19) Çetin, İsmail; Yeşilbağ, Derya; Cengiz, Şerife Şule; Belenli, Deniz; Uludağ Üniversitesi/Veteriner Fakültesi/Hayvan Besleme ve Beslenme Hastalıkları Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Biyokimya Anabilim Dalı.; 0000-0003-0708-3833; AAK-5370-2020; B-1526-2018; 56707038100; 9246817500; 56329475100; 56743270100The current study was conducted to evaluate the effects of dietary supplementation with rosemary volatile oil on performance, meat quality and selected plasma antioxidant parameters of quails. A total of 192 1-day-old Pharaoh (Coturnix coturnix Pharaoh) quails, including both males and females, were divided into three groups containing 64 quails and treated as follows: (1) a control group with 0 mg volatile oil/kg of diet; (2) 200 mg/kg rosemary volatile oil plus-RVO1; and (3) 250 mg/kg rosemary volatile oil plus-RVO2. The diets were prepared fresh for each treatment. The experiment was carried out for 42 days. Dietary treatments did not have any significant effect on live weight gain, feed intake, feed conversion rate, hot and cold carcass yield. There were significant differences for the plasma MDA level (P < 0.01) and meat MDA level (P <= 0.001) between the control and treatment groups. Plasma SOD activity not affected by addition of rosemary volatile oil but plasma GPx level was significantly (P < 0.01) affected by dietary treatments. In conclusion, rosemary volatile oil may be a potential natural antioxidant for quails and used to retard lipid oxidation in animal diets to improve meat products quality and animal performance.Item Influence of various volatile oils as a dietary supplement on biochemical and performance parameters in broilers(Uludağ Üniversitesi, 2015-04-28) Belenli, Deniz; Udum, Duygu; Cengiz, S. Şule; Polat, Ümit; Uludağ Üniversitesi/Veteriner Fakültesi.Volatile oils like rosemary, thyme and fennel oils have positive effects on poultry health and yields. The aim of this study were to investigate the effects of volatile oils on some serum biochemical parameters, growth hormone activities and growth performance in broiler chickens. A total of 180 broilers were randomly divided into 6 groups and 3 replicates of 10 animals according to supplementation of thyme oil, rosemary oil, fennel oil, oil mixture 100 ppm dosage and oil mixture 200 ppm dosage. Serum growth hormone activity and glucose concentration were not affected by dietary addition of volatile oils according to groups. However, addition of rosemary oil, thyme oil and fennel oil showed an effect on decreased serum cholesterol and partially lipid concentrations in the present study. In conclusion, supplementation of volatile oils had impressive effects on serum total cholesterol and total lipids levels in broiler diets. Whereas, growth performance results were not affected by dietary addition of volatile oils in our study.Item Kıvırcık ırkı koyunlara ait bazı biyokimyasal değerlerin belirlenmesi üzerine bir araştırma(Uludağ Üniversitesi, 2014-03-26) Udum, Duygu; Üstüner, Hakan; Belenli, Deniz; Uzabacı, Ender; Uludağ Üniversitesi/Veteriner Fakültesi.Bu çalışmanın amacı Kıvırcık ırkı koyunlara ait kan örneklerinde bazı biyokimyasal parametrelerin belirlenmesidir. Araştırma, Manisa ili ilçelerinde yetiştirilen 5 farklı çiftlikten oluşan, 1-6 yaş aralığında, klinik olarak sağlıklı, 30 adet Kıvırcık ırkı koyunlarında yapıldı. Bu hayvanlara ait plazma ve serum örneklerinde, total kolesterol, total protein, trigliserit, fosfolipit, kalsiyum, inorganik fosfor ve glikoz, ticari spektrofotometrik kitler kullanılarak spektrofotometre yardımıyla belirlendi. Kıvırcık ırkı koyunlar arasında çiftliklere göre istatistiksel farkın anlamlı olmadığı saptandı (p<0.05). Bu araştırma bu ırk ile yapılacak olan diğer çalışmalar için temel teşkil edecek niteliktedir.Item Obestatin and ghrelin may have a complementary function during acute and chronic period in mice(Bentham Science Publishers, 2016) Udum, Duygu; Belenli, Deniz; İlhan, Tuncay; Güneş, Nazmiye; Sonat, Füsun; Yalçın, Murat; Uludağ Üniversitesi/Veteriner Fakültesi/Biyokimya Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Histoloji Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Fizyoloji Anabilim Dalı.; 0000-0002-5600-8162; 0000-0001-7052-1694; AAG-6956-2021; AAH-8859-2021; AAI-1930-2021; AAH-5167-2021; EOC-6269-2022; IOT-8965-2023; 31967936400; 56743270100; 16549312600; 6506008074; 26428428000; 57192959734Obestatin is described as an anorexigenic peptide, and has adverse effects of ghrelin. It has no inhibitory effects on acute/chronic food intake, and it has been reported by several researchers. The role of obestatin in metabolism is still not clear. In the present study, the purpose is to determine the effects of chronically administrated obestatin. For this purpose, (1 mu mol/kg; i. p.) or ghrelin (1 mu mol/kg; i. p.) and food restriction (24h fast: 24h fed) on plasma obestatin, ghrelin, leptin, insulin, cholecystokinin (CCK) and glucose levels, and body weight gain were investigated for 14 days in mice. Additionally, mice were treated with acute ip (100 nmol/kg) injections of obestatin or ghrelin to investigate the food consumptions, plasma obestatin and ghrelin levels to determine unknown acute effects of obestatin. Plasma ghrelin levels increased significantly in obestatin administered mice when compared with the control group for chronic treatment. This increase is consistent with immunohistochemical findings which claim that the number of ghrelin and obestatin immunopositive cells in fundus tissue of stomach are considerably high in obestatin treated animals. Plasma obestatin and ghrelin levels has shown an increase endogenously in food restricted mice, but plasma leptin and insulin levels have been found to be lower compared to the control group. Acute administration of obestatin caused a decrease in plasma obestatin level at 60 min after injection and had no effect on the reduction of food intake in each treatment time. These results imply that obestatin may not itself be involved in the metabolism regulation; however, obestatin accompanied by ghrelin may play a role in the long- term regulation of metabolism.Item Stress responses of honey bees to organic acid and essential oil treatments against varroa mites(Taylor & Francis, 2016-12-29) Hranitz, John M.; Mengilig, Sami; Selova, Semih; Güneş, Nazmiye; Aydın, Levent; Belenli, Deniz; Uludağ Üniversitesi/Veteriner Fakültesi/Biyokimya Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Parazitoloji Anabilim Dalı.; AAI-1930-2021; 6506008074; 55808198600; 56743270100Natural medicines such as formic acid, oxalic acid, thymol and menthol have recently been used as alternative treatments against the honey bee parasitic mite varroa. The aim of this study was to observe and examine their impact on Heat Shock Proteins (HSP 70) in the brain tissues of bees. For this purpose, seven different treatment groups were created using various concentrations and administrations of formic and oxalic acid and thymol-menthol mixtures, including positive (untreated) and negative controls. The results showed that in the groups exposed to the varroa treatments, HSP 70 results were lower than those of the non-treated groups. HSP 70 results were lowest in the thymol-menthol mixture exposed group among the treated groups. We conclude that it would be more beneficial to prefer natural medicines against varroa, which lead to lower HSP 70 results which are the molecular determinants of stress.Item Tavuklarda glukosinolat ve hidroliz ürünlerinin enerji dengeleri ve performans parametreleri üzerine etkileri(Uludağ Üniversitesi, 2015-12-11) Belenli, Deniz; Polat, Ümit; Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü/Veteriner Biyokimya Anabilim Dalı.Tavuklarda glukosinolat ve hidroliz ürünlerinin enerji dengeleri ve performans parametreleri üzerine etkilerini incelemek amacıyla yapılan çalışma, 624 adet Ross-308 etçi ırk civciv kullanılarak gerçekleştirildi. 0 gün ile 42. günler arasında bakım ve besleme yapıldı. Civcivler, 1 kontrol ve 3 deneme grubu olmak üzere 4 ana grup ve bunların 3 tekrarlı ve dişi-erkek grupları olarak 24 gruba ayrıldı. Yemlere, yem katkı maddesi olarak tere tohumu (Lepidium sativum) eklendi. 1. deneme grubuna % 0,05, 2. deneme grubuna % 0,10 ve 3. deneme grubuna ise % 0,15 etken madde tere tohumu eklendi. Hayvanların canlı ağırlıkları, canlı ağırlık artışları, yem tüketimi ve yemden yararlanma oranları hesaplandı. Çalışmanın 0., 21. ve 42. günlerinde her gruptan 10 adet olacak şekilde kan örnekleri alındı. Serum örneklerinde, glukoz, büyüme hormonu, kortizol, östrojen, adiponektin ve leptin konsantrasyonları ölçüldü. Kesim günü olan 42. günde karkas ağırlıkları tartıldı, TBA analizi için 40 adet göğüs eti ve histolojik analizler için organlar toplandı. Elde edilen bulgular istatistiki olarak değerlendirildi. Biyokimyasal parametrelerden serum adiponektin, büyüme hormonu, glukoz, kortizol, leptin ve östrojen seviyeleri özellikle 21. ve 42. günlerde erkek ve dişi hayvanlarda kontrol ve deneme grupları arasında istatistiksel olarak p<0,05 düzeyinde önem bulunmuştur. Sonuç olarak; etçi tavuklara verilen tere tohumunun % 0,05, 0,10 ve 0,15 oranlarının performans (özellikle canlı ağırlık ile canlı ağırlık kazancı) ve karkas parametreleri üzerine etkilerinin olmadığı, fakat yem tüketimini % 0,10 oranında tere tohumu eklenen grup 2 dişilerinde azalttığı; yemden yararlanma oranlarının % 0,15 oranında eklenen grup 3 erkeklerinde anlamlı derecede artırdığı tespit edilmiştir. Histolojik parametreler incelendiğinde duodenumun villus intestinalis dokusunun uzunluklarında ve duodenuma ait kriptler derinliğinde 21.ve 42. günlerde kontrol grubuna deneme gruplarında artış görüldü. Aynı zamanda, % 0,10 ve 0,15 oranında etken maddenin eklendiği grup 2 ve 3 dişi ve erkeklerinde MDA düzeylerinde azalma görülmüş ve bu oranların özellikle ticari anlamda etçi tavukların kesildikten sonra raf ömrünü artırmada yardımcı olacağı şeklinde yorumlanmıştır.