Browsing by Author "Alcay, Selim"

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Characterisation of genital mycoplasma species from preputial swabs of bucks and rams
(Kafkas Univ, Veteriner Fakultesi Dergisi, 2020-03-01) Göçmen, Huban; Rosales, Ruben S.; Ridley, Anne; Alcay, Selim; ALÇAY, SELİM; Bursa Uludağ Üniversitesi/Veteriner Fakültesi; JJQ-9411-2023
Mycoplasma species are a major cause of mastitis, arthritis, pneumonia and reproductive disorders in small ruminants. Mycoplasmas in reproductive systems of males have been associated with diseases as orchitis, balanoposthitis and abnormal spermatozoa activity. The aim of this study was to detect Mycoplasma species that cause reproductive infections in bucks and rams. Total of 27 preputial swabs was collected from Saanen bucks and Kivircik rams at the Artificial Insemination (Al) Center of Uludag University in Turkey. Bacteriological culture methods, followed by Polymerase Chain Reaction (PCR) and Denaturing Gradient Gel Electrophoresis (DGGE) were used to detect and identify Mycoplasma species. The PCR-DGGE method identified one M. bovigenitalium and one M. arginini from two cases of orchitis in rams, and another M. bovigenitalium was identified from a buck with no clinical signs. The results showed that Mycoplasma species were present in the testicles of rams and bucks that were negative for Brucellosis, and likely causative organisms of orchitis which lead to reduced fertility.
Comparison and assessment of ovarian follicular dynamics during the breeding and non-breeding season in saanen goats
(B W K Publishing Solutions & Verlag, 2020-01-01) Dogan, İbrahim; DOĞAN, İBRAHİM; Toker, Mehmed Berk; TOKER, MEHMED BERK; Alcay, Selim; ALÇAY, SELİM; Küçüksen, D. Udum; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Biyokimya Bölümü.; 0000-0003-1976-1814; A-2794-2014; R-8366-2018
We describe and compare the ovarian follicular dynamics and plasma progesterone concentrations in nulliparous Saanen does during the breeding and non-breeding seasons. Does were monitored daily using transrectal ovarian ultrasonography for a full oestrous cycle in the breeding (n = 19) and a 21-day period in the non-breeding season (n = 20). At two-day intervals, blood samples were taken to determine plasma progesterone by ELISA. The pattern of follicular growth was characterised by four and five follicular waves in both seasons. Although the number of follicular waves was similar in the two seasons, the number of codominant follicles (p=0.027), the diameter of the largest follicle of waves 1 (p=0.036) and 4 (p=0.031), the duration of waves 3 (p=0.029) and 4 (p=0.034), the diameter of the largest follicle (p=0.042), the sums of the durations of all waves (p=0.037), the interwave interval (p=0.026) and the follicle growth rate (p=0.044) were higher in the breeding than in the non-breeding season. In addition, the numbers of small, medium and large follicles and the total number of all follicles of all waves differed between the seasons (p=0.037). In the breeding season, we found significant differences between the waves in the diameter of the largest follicles (p=0.042) and the duration of the waves (p=0.037); in the non-breeding season, no significant differences were found. Ovulatory follicles were most common in the fifth follicle wave. In the breeding season, the mean inter-oestrus and inter-ovulatory intervals and the numbers of ovulations after a synchronized oestrus with PGF2a were 21.47 +/- 0.19 days, 20.47 +/- 0.19 days and 1.84 +/- 0.86. The results indicate that follicular growth in Saanen goats during the breeding and non-breeding season is characterised by a wave-like pattern that differs between seasons.
Comprehensive effects of fetal calf serum in soybean-lecithin based goat semen cryopreservation extenders and impacts on incubation resilience
(Kafkas Univ, Veteriner Fakultesi Dergisi, 2022-06-24) Toker, Mehmed Berk; TOKER, MEHMED BERK; Alcay, Selim; ALÇAY, SELİM; Bursa Uludağ Üniversitesi/Veteriner Fakültesi.; A-2794-2014
The aim of this study was to investigate the effects of various fetal calf serum (FCS) doses on %1 soybean lecithin-based semen extenders for goat semen cryopreservation and the impacts on spermatological parameters over post-thaw and post-incubation (6 h) time periods. Sperm samples collected via electro-ejaculation method were pooled to overcome the individual differences and were used in the study. The samples then were split into four equal aliquots to create study groups as; 0.25%, 0.50%, 0.75% FCS supplemented and a sample of FCS-free control group. Each sample group was diluted to approximately 150 x 106 spermatozoon/mL final concentration and two step dilution method was used for cryopreservation. Study groups were examined for sperm motility, plasma membrane functional integrity with hypoosmotic swelling test (HOST), acrosome integrity by FITC-Pisum sativum agglutinin (PSA-FITC) and DNA damage by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay (TUNEL). All samples were incubated for further 6 h in a humidified air chamber with 5% CO2 at 39 degrees C. The results indicated that FCS supplementation in soybean lecithin-based extenders for goat sperm cryopreservation had significant effects on post-thawing time point motility (P < 0.05), plasma membrane integrity (P < 0.05) and acrosomal integrity (P < 0.05) parameters. Subsequently to 6 h of incubation period, DNA integrity results yielded better scores comparing to control group in addition with other spermatological parameters (P < 0.05).
Drone semen cryopreservation with protein supplemented tl-hepes based extender
(Kafkas Univ, Veteriner Fakultesi Dergisi, 2019-07-01) Alcay, Selim; ALÇAY, SELİM; Çakmak, Selvinar; Çakmak, İbrahim; ÇAKMAK, İBRAHİM; Mülkpınar, Emine; Toker, Mehmed Berk; TOKER, MEHMED BERK; Üstüner, Burcu; ÜSTÜNER, BURCU; Şen, Hasan; Nur, Zekariya; NUR, ZEKARİYA; Bursa Uludağ Üniversitesi/Veteriner Fakültesi; 0000-0002-1438-221X; AAH-2635-2021; AAH-2558-2021; AAG-7238-2021; A-2794-2014
The aim of the current study was to determine the optimum concentration of bovine serum albumin for post-thawing quality of drone sperm and this is the first study to evaluate the effect of BSA supplemented TL-Hepes based extenders for drone semen cryopreservation. Sexually mature drones were used for semen collection. Pooled semen was diluted with TL-Hepes based extender supplemented with different concentrations of BSA (1 mg/mL, 3 mg/mL, and 5 mg/mL) or without BSA (control), at a final concentration of 100x10(6) spermatozoon/mL. Motility, plasma membrane functional integrity (HOST), and defected acrosome (PSA-FITC) were evaluated in the study. At post thaw, the highest sperm motility rates were obtained in the BSA5 group (P< 0.05). Functional integrity of sperm membrane was better preserved in the BSA3 and BSA5 groups compared to the other groups. The acrosomal integrity rates were higher in BSA5 group than in control group (P< 0.05). The study shows that bovine serum albumin supplemented TL-Hepes based extenders have beneficial effect on drone semen parameters at post-thaw. The results of the study demonstrated a notable advantage of using 5 mg/mL of BSA in TL-Hepes based extender.
Effect of freezing rate on goat sperm morphology and DNA integrity
(TÜBİTAK, 2015) Üstüner, Burcu; Nur, Zekariya; Alcay, Selim; Toker, Mehmet Berk; Sağırkaya, Hakan; Soylu, Mustafa Kemal; Uludağ Üniversitesi/Veteriner Fakültesi/Dölerme ve Suni Tohumlama Anabilim Dalı.; 0000-0002-1438-221X; 0000-0003-4033-9749; AAG-7238-2021; AAH-2635-2021; CBC-7350-2022; A-2794-2014; AAH-8821-2021; HKG-7604-2023; 18937724600; 6508060684; 56099810300; 56480349200; 6602400461; 7003293300
This study investigates the effect of freezing rates on the spermatological parameters of frozen and thawed Saanen goat semen. Equilibrated semen was frozen at 4 different freezing rates from +5 degrees C to -150 degrees C (G10: 10 degrees C/min, G12: 12 degrees C/min, G15: 15 degrees C/min, and G24: 24 degrees C/min) and stored in liquid nitrogen until use. Semen samples were examined for sperm motility, defective acrosomes (FITC-PSA), and DNA integrity using TUNEL after dilution with extender A at equilibration and postthaw stage. There was no significant difference among the freezing stages in terms of DNA fragmentation (P > 0.05). DNA integrity was partially affected by the freezing rate. The increase of freezing rate from 10 degrees C/min to 24 degrees C/min between +5 degrees C and -150 degrees C resulted in higher postthaw DNA damage. The study found that the freeze-thawing process is detrimental to postthawed goat semen motility (P < 0.05), acrosome integrity (P < 0.05), and DNA integrity (P > 0.05). Although the freezing rates used in the present study had no effect on postthaw sperm motility and acrosome integrity (P > 0.05), sperm DNA integrity was affected.
Effect of gnrh treatment following a short-term estrous induction protocol on estrus and ovulation in saanen goats, during the transitional period
(Hellenic Veterinary Medical Soc, 2020-10-01) Doğan, İbrahim; DOĞAN, İBRAHİM; Toker, Mehmed Berk; TOKER, MEHMED BERK; Alcay, Selim; ALÇAY, SELİM; Küçüksen, Duygu Udum; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Biyokimya Anabilim Dalı.; 0000-0003-1976-1814; R-8366-2018; A-2794-2014
The objective of this study was to compare the effect on the ovulation time and estrus parameters of a GnRH agonist (buserelin acetate) administered 24 or 36 h following a short-term estrus induction and synchronization treatment in non-lactating Saanen goats during the transitional period. Goats received 20 mg FGA sponges for 6 days plus 300 IU eCG and 125 mu g d-cloprostenol 24 h prior to sponge removal. After removal of the sponges, goats were given either 1 ml physiological saline (0.9% NaCI) solution (Group 1; n = 9) after 12 h, 0.004 mg GnRH (Group 2; n = 10) after 24 h or 0.004 mg GnRH (Group 3; n = 10) after 36 h. The follicle development and ovulation in the ovaries were monitored by transrectal ultrasonography starting from the sponge application until the fifth day of the estrus cycle. Blood samples were collected on the same days to determine the plasma concentrations of progesterone (P-4) and estradiol (E-2). No statistical differences among groups were detected in any synchronization parameters, ultrasonic evaluations and plasma P-4 and E-2 concentrations. The evaluation of pooled data showed that the response of Saanen goats to treatments was comparable to the results of other published trials. Results of this study indicate that administration of GnRH at 24 h or 36 h after sponge removal, at the end of a short estrus induction and synchronization protocol, does not affect plasma P-4 and E-2 concentrations, estrus parameters and ovulation time in goats, during the transition period.
Effect of rainbow trout (Oncorhynchus mykiss) seminal plasma on the post-thaw quality of ram semen cryopreserved in a soybean lecithin-based or egg yolk-based extender
(Elsevier, 2015-11-15) Üstüner, Burcu; Alcay, Selim; Toker, M. Berk; Nur, Zekariya; Gökce, Elif; Sonat, Füsun Ak; Gül, Zülfiye; Duman, Muhammed; Cenize, Cafer; Uslu, Aydın; Saığrkaya, Hakan; Soylu, Mustafa Kemal; Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Fizyoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Su Hayvanları Hastalıkları Anabilim Dalı.; 0000-0001-7707-2705; 0000-0002-1438-221X; 0000-0002-8872-0074; 0000-0002-7678-3289; AAH-2635-2021; T-1697-2019; AAH-8821-2021; AAF-9939-2020; AAG-7238-2021; 18937724600; 56099810300; 56480349200; 6508060684; 56779799700; 26428428000; 56086542900; 55568071100; 57070339500; 57069822700; 6602400461; 7003293300
The aim of the current study was to evaluate the effects of different concentrations of rainbow trout seminal plasma (RTSP) (0.1%, 1% and 10%) in extenders containing either egg yolk or lecithin for use in Awassi ram semen cryopreservation. Pooled sperm were diluted in a two-step dilution method to a final concentration of 1/5 (semen/extender) in egg yolk or lecithin extender containing no RTSP, 0.1%, 1% or 10% RTSP (v/v). Semen samples were assessed for sperm motility, plasma membrane integrity [hypoosmotic swelling test (HOST) and Hoechst 33258] and defective acrosomes [FITC-conjugated Pisum sativum agglutinin (PSA-FITC)] at the following five time points: after dilution with extender A; after equilibration; and post-thaw at 0 h, 3 h and 5 h. Malondialdehyde (MDA) was examined only after thawing. Freezing and thawing procedures (dilution, equilibration and post-thaw incubation at Oh, 3 h and 5 h) negatively affected the motility (P<0.001) and acrosome integrity (P<0.001). Additionally, freezing and thawing negatively affected the plasma membrane integrity, as determined by the HOST and Hoechst 33258 (P<0.001). The extender group affected the motility (P<0.001) and the HOST results (P<0.001). Levels of MDA in the egg yolk extender with 1% RTSP group were significantly lower than in the lecithin control group (P<0.05). In conclusion, the egg yolk extender groups that were supplemented with 10% and 1% RTSP provided greater cryoprotective effects for semen survivability during 5 h incubation than the other extender groups.
Effects of low molecular weight cryoprotectants on the post-thaw ram sperm quality and fertilizing ability
(Elsevier, 2016-03) Alcay, Selim; Üstüner, Burcu; Nur, Zekariya; Uludağ Üniversitesi/Veteriner Fakültesi/Dölleme ve Suni Tohumlama Bölümü.; 0000-0002-1438-221X; AAH-2635-2021; AAG-7238-2021; 56099810300; 18937724600; 6508060684
The aim of the current study was to compare the cryoprotective effects of low molecular weight cryoprotectants 6% DMSO, 6% ethylene glycol, 6% 1,2 propanediol and 6% glycerol on the efficiency of ram semen cryopreservation and to test the in vitro fertilizing ability of frozen-thawed ram semen. Ejaculates with a thick consistency, rapid wave motion (3-5), and >75% initial motility were pooled. Sperm were diluted to a final concentration of 1/5 (semen/extender) in 6% glycerol, 6% DMSO, 6% ethylene glycol and 6% 1,2 propanediol using a two-step dilution method. Two hours equilibrated semen was frozen in 0.25-ml straws. As expected, the results of the current study showed that motility, HOST values and the rates of defective acrosomes in sperm were negatively affected by the cryopreservation procedure (P<0.01). In conclusion, 6% DMSO had a deleterious effect on the post thaw ram sperm parameters and embryonic development. Ethylene glycol and 1,2 propanediol successfully protected acrosomal and DNA integrities. Glycerol provided a better cryoprotection to sperm motility and plasma membrane integrity. The results of in vitro fertilization, as assessed by the rate of blastocyst formation, in glycerol group were similar to 1,2 propanediol group.
Ovarian follicle dynamics and hormonal changes during early pregnancy in saanen goats
(Czech Academy Agricultural Sciences, 2020-01-01) Doğan, İbrahim; Toker, Mehmed Berk; Alcay, Selim; Küçükşen, Duygu Udum; DOĞAN, İBRAHİM; TOKER, MEHMED BERK; ALÇAY, SELİM; UDUM, DUYGU; 0000-0003-1976-1814; 0000-0003-4033-9749; A-2794-2014; R-8366-2018; CBC-7350-2022; FGX-3813-2022
This study is aimed at describing the ovarian follicular dynamics, corpora lutea and their hormonal control during early pregnancy in Saanen goats. The ovaries of pregnant goats (n = 10) were monitored daily for follicles (>= 2 mm) and corpora lutea by transrectal ultrasound over the 35 days after mating. Blood samples were collected by jugular venipuncture at the time of ultrasonography and were analysed for the concentrations of oestradiol (E-2) and progesterone (P-4), inhibin, FSH (follicle-stimulating hormone) and LH (luteinizing hormone). The pattern of follicular growth was characterised by five (n = 2; 20 %), six (n = 5; 50 %) and seven (n = 3; 30 %) follicular waves during early pregnancy. The first corpora lutea (size 6.8 +/- 0.4 mm, mean + SEM) were detected at 5.0 +/- 0.3 days after the mating with a maximum diameter (12.2 +/- 0.3 mm) observed on day 24.0 +/- 1.1 of pregnancy. The dominant follicle diameter of waves 1 and 4, and the duration of waves 1-4 and 7 were maximal during the early pregnancy period (P < 0.05). In addition, the inter-wave interval of the 5 waves was significantly (P < 0.05) longer compared to the six and seven inter-wave intervals (7.0 +/- 0.1, 5.8 +/- 0.1 and 5.0 +/- 0.2 days, respectively). Although the number of small and medium-sized follicles did not differ with the days post-breeding, the number of large-sized follicles in the same period significantly decreased between 16 and 26 days and was negatively correlated with the period of pregnancy (P < 0.05). The plasma LH and E-2 concentrations were negatively correlated with the P-4 concentration. The inhibin concentrations were negatively correlated with FSH, but positively correlated with the number of large-sized follicles. These results indicate that ovarian follicular growth during the first 35 days of pregnancy in goats was characterised by a wave-like pattern and there was a close relationship between the number of FSH peaks and the number of follicular waves.
Successful cryopreservation of honey bee drone spermatozoa with royal jelly supplemented extenders
(Academic Press, 2019-04) Gökçe, Elif; Alcay, Selim; Çakmak, Selvinar; Cakmak, İbrahim; Mülkpınar, Emine; Üstüner, Burcu; Şen, Hasan; Nur, Zekariya; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Klinik Bilimler/Dölenme ve Suni Tohumlama Bölümü.; 0000-0002-1438-221X; AAH-2635-2021; AAG-7238-2021; AAH-2558-2021; 56099810300; 55184998300; 57207796431; 57207740686; 18937724600; 57207740868; 6508060684
The aim of the present study was to evaluate different concentrations of royal jelly (RJ) supplemented extenders for post-thawing quality of drone sperm. Semen samples were collected from sexually mature drones. Pooled semen was diluted with extender without RJ (control) or supplemented with different concentrations of RJ (1, 2, 4 or 8%). Sperm motility, plasma membrane functional integrity, and acrosomal integrity were evaluated. At post thaw, the highest sperm motility and acrosomal integrity rates were obtained in the RJ1 group. Functional integrity of sperm membrane was better preserved in the RJ1 and RJ2 groups compare to the other groups. The study shows that RJ supplemented extenders have beneficial effects on drone semen parameters. The results of the present study demonstrated advantage of using 1% RJ supplemented extender.