Bruselloz tanı yöntemlerinin etkinliğinin araştırılması
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Date
2005-10-18
Journal Title
Journal ISSN
Volume Title
Publisher
Uludağ Üniversitesi
Abstract
Çalışmamızda, Haziran 2000-Mayıs 2003 tarihleri arasında anamnez ve fizik muayene bulgularıyla bruselloz olduğu düşünülen, Rose Bengal lam aglütinasyon testi (RBT) ve Wright tüp aglütinasyon testi (TAT) pozitif bulunarak tanısı kesinleşen 24’ü erkek, 8’i kadın hastada bruselloz tanı yöntemlerinin etkinliği araştırılmıştır. Olgularımızın %100’ünde RBT ve TAT, 12 (%37.5) hastada Brucella IgM pozitif bulundu. ELISA yöntemiyle bakteriye karşı oluşan antikorların tespitinin, hastalığın tanı ve tedavisinin takibinde anlamlı olduğu görüldü. Hastalarımızın %18.7’sinde kanda, %28.1’inde kemik iliğinde üreme saptandı. Kültür sonuçlarının düşük kalmasının nedeni, hastalara tanı konmadan önce başka sağlık kuruluşları tarafından verilen çeşitli antibiyotikler olarak düşünüldü. Çalışmamızda, 3 (%9) olguda PCR tekniği ile serumda Brucella bakterisi saptandı. Hasta serumunun PCR tekniği için uygun örnek olmadığı düşünüldü.
In our study, 24 male and 8 female patients with brucellosis whose diagnosis were confirmed by positive slide agglutination (Rose Bengal) and standard tube agglutination (Wright) tests were enrolled between the dates of June 2000 and May 2003. Both agglutination tests were positive in all patients. In the serological diagnosis of brucellosis, Brucella IgM antibodies detected by using ELISA were positive in 12 (37.5%) patients respectively. Detection of the antibodies by using ELISA method was found to be effective in the diagnosis and follow-up to response to the treatment of Brucella. Blood and bone marrow cultures were positive in 18.75% and 28.1% of patients, respectively. Non-specific antibiotic use before specific diagnosis and treatment was thought as a cause of low positivity rate in culture results. Because polymerase chain reaction (PCR) assay was positive only in 3 (%9) patients, the serum was considered not to be the appropriate material for the analysis
In our study, 24 male and 8 female patients with brucellosis whose diagnosis were confirmed by positive slide agglutination (Rose Bengal) and standard tube agglutination (Wright) tests were enrolled between the dates of June 2000 and May 2003. Both agglutination tests were positive in all patients. In the serological diagnosis of brucellosis, Brucella IgM antibodies detected by using ELISA were positive in 12 (37.5%) patients respectively. Detection of the antibodies by using ELISA method was found to be effective in the diagnosis and follow-up to response to the treatment of Brucella. Blood and bone marrow cultures were positive in 18.75% and 28.1% of patients, respectively. Non-specific antibiotic use before specific diagnosis and treatment was thought as a cause of low positivity rate in culture results. Because polymerase chain reaction (PCR) assay was positive only in 3 (%9) patients, the serum was considered not to be the appropriate material for the analysis
Description
Keywords
Bruselloz, Tanı testleri, Brucellosis, PCR, Diagnostic Tests
Citation
Dizer, U. vd. (2005). ''Bruselloz tanı yöntemlerinin etkinliğinin araştırılması''. Uludağ Üniversitesi Tıp Fakültesi Dergisi, 31(2), 87-93.