İmmobilizasyon stresinin plazma lipit ve lipoprotein fraksiyonları üzerine etkisi
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Date
1997-01-27
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Uludağ Üniversitesi
Abstract
Bu çalışmada 30 gün immobilizasyon stresi uygulanan Wistar türü sıçanların lipit ve lipoprotein fraksiyonları incelendi.Çalışma 3 grupta yapıldı: Kontrol grubu (grup 1), grup 2 ve grup 3. Her grupta 8 sıçan bulunmaktaydı. Grup 2 ve 3 'e 30 gün süresince sırasıyla, günde 5 ve 16 saat immobilizasyon stresi uygulandı. Stres işlemi sonunda plazma total kolesterol (TK), trigliserit (TG), lipoprotein elektroforez fraksiyonları (a, preß ve ß) ve kortikosteron düzeyleri ölçüldü. Grup 2 ve grup 3 kontrol grubu ile karşılaştırıldığında TK düzeyinde anlamlı bir fark gözlenmedi ancak grup 3 'ün TK düzeyleri grup 2 ye göre anlamlı (p < 0. 001) olarak düşük olduğu görüldü. TG düzeyi 2. grupta, kontrol grubuna göre ve 3. gruba göre anlamlı olarak daha düşüktü. Lipoprotein elektroforezi de 3. grup kontrol grubu ile karşılaştırıldığında % a-lipoprotein ve a-lipoprotein -kolesterol (a-Lp-K) 'ün daha düşük; % preß-Lp ve TK/ a-Lp-K oranının daha yüksek olduğu saptandı. Kortikosteron düzeyinin grup 2 'de kontrol grubuna göre anlamlı olarak (p < 0. 001) daha yüksek olduğu bulundu. Kortikosteron plazma tiplerinin korelasyonları incelendi ve kontrol grubunda kortikosteron kolesterol ve aLp-K arasında anlamlı pozitif korelasyonlar (sırasıyla r = 0. 79 ve r = 0. 72) olduğu saptandı. Sonuçta daha uzun süren immobilizasyon stresinin a-Lp-K değerini düşürüp TK/a-Lp-K oranını artırarak lipoprotein metabolizması etkilediği sonucuna varıldı .
In this study, the effects of immobilization stress over a period of 30 days on plasma lipids and lipoprotein fractions have been investigated in male Wistar-strain rats. In each of the experimental groups (group 2 and 3) and the controls (group 1), there were eight rats. The experimental animals in group 2 and 3 have been subjected to immobilization stress for 5 and 16 hours a day respectively. At the end of the stress treatment, plasma total cholesterol (TC), triglyceride (TG), lipoprotein (Lp) electrophoresis fractions ( a, pre B,B) and corticosterone levels have been measured. In the experimental groups, TC values were not more different than the control group 's value. However, it was found to be significantly lower (p < 0. 001) in group 3 when compared to group 2. TG was significantly lower in group 2 compared to control group and group 3 levels. Lp electrophoresis patterns in group 3 when compared to normal ones showed a different redistribution of percentage values and cholesterol contents as folloıving : a-Lp % and a-Lp-cholesterol (a-Lp-C) were tower white pre B-Lp% and TC/ a-Lp-C ratio higher. Corticosterone was significantly (p < 0. 001) higher in group 2 when compared to the control group. The correlations between corticosterone and plasma lipids were evaluated. There were statistically positive correlations between corticosterone and TC and a-Lp-C (r = 0. 79 and r = 0. 72 respectively) in the control group. In the end, it has been concluded that in the group in which immobilization stress lasts longer, stress has influenced lipoprotein metabolism by towering a-Lp-C and increasing the ratio of TC/a-Lp-C.
In this study, the effects of immobilization stress over a period of 30 days on plasma lipids and lipoprotein fractions have been investigated in male Wistar-strain rats. In each of the experimental groups (group 2 and 3) and the controls (group 1), there were eight rats. The experimental animals in group 2 and 3 have been subjected to immobilization stress for 5 and 16 hours a day respectively. At the end of the stress treatment, plasma total cholesterol (TC), triglyceride (TG), lipoprotein (Lp) electrophoresis fractions ( a, pre B,B) and corticosterone levels have been measured. In the experimental groups, TC values were not more different than the control group 's value. However, it was found to be significantly lower (p < 0. 001) in group 3 when compared to group 2. TG was significantly lower in group 2 compared to control group and group 3 levels. Lp electrophoresis patterns in group 3 when compared to normal ones showed a different redistribution of percentage values and cholesterol contents as folloıving : a-Lp % and a-Lp-cholesterol (a-Lp-C) were tower white pre B-Lp% and TC/ a-Lp-C ratio higher. Corticosterone was significantly (p < 0. 001) higher in group 2 when compared to the control group. The correlations between corticosterone and plasma lipids were evaluated. There were statistically positive correlations between corticosterone and TC and a-Lp-C (r = 0. 79 and r = 0. 72 respectively) in the control group. In the end, it has been concluded that in the group in which immobilization stress lasts longer, stress has influenced lipoprotein metabolism by towering a-Lp-C and increasing the ratio of TC/a-Lp-C.
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Keywords
İmmobilizasyon stresi, Lipitler, Sıçan, Immobilization stress, Lipids, Rat
Citation
Dirican, M. vd. (1996). ''İmmobilizasyon stresinin plazma lipit ve lipoprotein fraksiyonları üzerine etkisi''. Uludağ Üniversitesi Tıp Fakültesi Dergisi, 23(1-2-3), 5-9.