Antalya ili kabak üretim alanlarında Fas Karpuz Mozayik Virüsü (Moroccan Watermelon Mosaic Virus)
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Date
2022-06-01
Authors
Kılıç, Handan Çulal
Oran, Muhammed Raşit
Journal Title
Journal ISSN
Volume Title
Publisher
Bursa Uludağ Üniversitesi
Abstract
Bu çalışma Antalya ili kabak üretim alanlarından toplanan kabak örneklerinde Fas karpuz mozayik virüsü (Moroccan watermelon mosaic virus; MWMV)’nün tespit edilmesi amacıyla 2019-2020 yıllarında yürütülmüştür. MWMV’nün varlığı serolojik ve moleküler yöntemlerle belirlenmiştir. Survey çalışmalarında virüs simptomu sergileyen 120 yaprak örneği alınmış, örneklere DAS-ELISA (Double Antibody Sandwich Enzyme Linked Immunosorbent Assay) yöntemi uygulanmıştır. DAS-ELISA testi sonucunda Aksu, Manavgat ve Kumluca ilçelerinden toplanan 9 yaprak örneğinde MWMV belirlenmiştir. RT-PCR (Reverse Transcriptase Polymerase Chain Reaction) çalışmalarında enfekteli bu 9 yaprak örneği kullanılmıştır. RT-PCR uygulamasında MWMV kılıf protein geni için spesifik primerler kullanılarak yaklaşık 627 bç’lik bir kısım amplifiye edilmiş ve beklenen seviyede bant elde edilmiştir. Bu çalışma, Antalya ili kabak alanlarında MWMV’nin serolojik ve moleküler yöntemlerle tanılanması bakımından ilk çalışma niteliğindedir.
This study was carried out to detect the presence of Moraccon watermelon mosaic virus (MWMV) in squash collected from Antalya province during 2019-2020. The presence of MWMV was determined by serological and molecular methods. A total of 120 leaf samples was collected from plants showing diseases symptoms. Plant samples were tested by Double Antibody Sandwich Enzyme Linked Immunosorbent Assay (DAS-ELISA) method. According to DAS-ELISA test results, it was observed that 9 samples out of 120 collected from of Aksu, Manavgat and Kumluca districts of Antalya province were infected with MWMV. 9 infected samples were tested by Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). RT-PCR test showed that bands about 627 bp were amplified from samples using primers spesific for coat protein gene (CP) of MWMV. This study is the first time in detection of MWMV by serological and molecular methods squash areas of Antalya province.
This study was carried out to detect the presence of Moraccon watermelon mosaic virus (MWMV) in squash collected from Antalya province during 2019-2020. The presence of MWMV was determined by serological and molecular methods. A total of 120 leaf samples was collected from plants showing diseases symptoms. Plant samples were tested by Double Antibody Sandwich Enzyme Linked Immunosorbent Assay (DAS-ELISA) method. According to DAS-ELISA test results, it was observed that 9 samples out of 120 collected from of Aksu, Manavgat and Kumluca districts of Antalya province were infected with MWMV. 9 infected samples were tested by Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). RT-PCR test showed that bands about 627 bp were amplified from samples using primers spesific for coat protein gene (CP) of MWMV. This study is the first time in detection of MWMV by serological and molecular methods squash areas of Antalya province.
Description
Keywords
Fas Karpuz Mozayik Virüsü, Kabak, Teşhis, Moroccan Watermelon Mosaic Virus, Squash, Detection
Citation
Kılıç, H. Ç. ve Oran, M. R. (2022). "Antalya ili kabak üretim alanlarında Fas Karpuz Mozayik Virüsü (Moroccan Watermelon Mosaic Virus)". Bursa Uludağ Üniversitesi Ziraat Fakültesi Dergisi, 36(1), 15-24.