Electrophoretic separation of urine proteins of healthy dogs and dogs with nephropathy and detection of some urine proteins of dogs using immunoblotting

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dc.contributor.buuauthorYalçın, Abdullah
dc.contributor.buuauthorÇetin, Meltem
dc.contributor.departmentUludağ Üniversitesi/Veteriner Fakültesi/Bi̇yoki̇mya Anabi̇li̇m Dalı.tr_TR
dc.contributor.scopusid36857831000tr_TR
dc.contributor.scopusid57197086676tr_TR
dc.date.accessioned2022-04-21T06:41:48Z
dc.date.available2022-04-21T06:41:48Z
dc.date.issued2004-02
dc.description.abstractDuring nephropathy, the presence of proteins in urine of dogs is relatively frequent. The detection and characterization of proteinuria may be an important help to establish diagnosis and prognosis in renal diseases. In 50 dogs, 25 of which were healthy (healthy group) and 25 of which were with nepropathy (patient group), the urine proteins and proteinuria were evaluated by urine proteine to creatinine ratio of concentrations (UPr/Cr), sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The UPr/Cr ratio concentrations was significantly increased in patient dogs in comparison to control group (0.558 +/- 0.425 and 0.041 +/- 0.026, p < 0.05 respectively) and 21 dogs of the patient group (84 %) had a UPr/Cr ratio above 0.2, indicating a pathological proteinuria. At least, 10 different bands were evidenced by SDS-PAGE and were divided into 3 groups: LMW (low molecular weight) proteins (below 66 kiloDaltons-kD), MMW (middle molecular weight) proteins (66-76 kD) and HMW (high molecular weight) proteins (above 76 kD). In patient group, 2 types of proteinuria were obtained : Glomerular tubular proteinuria characterized by presence of HMW or MMW and LMW in 67 % of dogs having a marked proteinuria and tubular proteinuria characterized by the presence of only LMW in 33 % of dogs. In healthy dogs, only scarce bands were visiualised by SDS-PAGE. By immunoblotting, transferrin, alpha(1)-microglobulin, beta(2)-microglobulin and retinol binding protein (RBP) were more frequently detected in patient dogs than in healthy dogs (p < 0.05). Moreover, RBP was detected in all the patient dogs with proteinuria, whereas it was detected only in the 2 of the healthy dogs, and this protein could be relatively specific for tubular damage.en_US
dc.identifier.citationYalçın, A. ve Çetin, M. (2004). “Electrophoretic separation of urine proteins of healthy dogs and dogs with nephropathy and detection of some urine proteins of dogs using immunoblotting”. Revue De Medecine Veterinaire, 155(2), 104-112.en_US
dc.identifier.endpage112tr_TR
dc.identifier.issn0035-1555
dc.identifier.issue2tr_TR
dc.identifier.scopus2-s2.0-17044457504tr_TR
dc.identifier.startpage104tr_TR
dc.identifier.urihttp://hdl.handle.net/11452/25928
dc.identifier.volume155tr_TR
dc.identifier.wos000220918200008tr_TR
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherEcole Nationale Veterinaire Toulousefre
dc.relation.journalRevue De Medecine Veterinairefre
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectVeterinary sciencesen_US
dc.subjectAnimaliaen_US
dc.subjectCanis familiarisen_US
dc.subjectGlomerulusen_US
dc.subjectDogen_US
dc.subjectUrine proteinsen_US
dc.subjectProteinuriaen_US
dc.subjectElectrophoresisen_US
dc.subjectImmunoblottingen_US
dc.subjectNephropathyen_US
dc.subjectRetinol-binding-proteinen_US
dc.subjectPolyacrylamide gel-electrophoresisen_US
dc.subjectTo-creatinine ratioen_US
dc.subjectSDS-PAGEen_US
dc.subjectExcretionen_US
dc.subjectAlbuminen_US
dc.subject.emtreeAnimal experimenten_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeConcentration (parameters)en_US
dc.subject.emtreeControlled studyen_US
dc.subject.emtreeDiagnostic procedureen_US
dc.subject.emtreeDisease classificationen_US
dc.subject.emtreeDog diseaseen_US
dc.subject.emtreeEvaluationen_US
dc.subject.emtreeFemaleen_US
dc.subject.emtreeGlomerulusen_US
dc.subject.emtreeHealth statusen_US
dc.subject.emtreeImmunoblottingen_US
dc.subject.emtreeKidney diseaseen_US
dc.subject.emtreeKidney tubuleen_US
dc.subject.emtreeMaleen_US
dc.subject.emtreeMolecular weighten_US
dc.subject.emtreeNonhumanen_US
dc.subject.emtreePathologyen_US
dc.subject.emtreePolyacrylamide gel electrophoresisen_US
dc.subject.emtreeProtein analysisen_US
dc.subject.emtreeProtein urine levelen_US
dc.subject.emtreeProteinuriaen_US
dc.subject.emtreeSeparation techniqueen_US
dc.subject.emtreeStatistical significanceen_US
dc.subject.emtreeUrinalysisen_US
dc.subject.emtreeAlpha 1 microglobulinen_US
dc.subject.emtreeBeta 2 microglobulinen_US
dc.subject.emtreeCreatinineen_US
dc.subject.emtreeRetinol binding proteinen_US
dc.subject.emtreeTransferrinen_US
dc.subject.scopus6 N,N' Dimethylarginine; Oscillometry; Catsen_US
dc.subject.wosVeterinary sciencesen_US
dc.titleElectrophoretic separation of urine proteins of healthy dogs and dogs with nephropathy and detection of some urine proteins of dogs using immunoblottingen_US
dc.typeArticle
dc.wos.quartileQ4en_US

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