Serum aluminyum tayininde standart eğri grafiği ve standart ekleme yöntemlerinin kullanımı
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Date
1995-11-29
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Uludağ Üniversitesi
Abstract
Bu çalışmada; serumda aluminyum (Al) tayini için standart eğri grafiği (StEG) ve standart ekleme (StE, ekstrapolusyon) yöntemleri atomik absorpsiyon spektrofotometre cihazında çalışılarak, incelendi. Ayrıca Al ölçümü yapılacak, serumlara 3 değişik teknikle ön hazırlık uygulandı. Bunlar; serumun direkt kullanımı, matriks değiştirici (MD) ile dilüe edilmesi ve protein presipitasyonu (PP) teknikleriydi. Bunlar da yöntemler içinde birbirleriyle kıyaslandı StEG yöntemiyle direkt serum (n=20) ve PP yapılarak kullanılan serumlarda (n=20) ortalama Al düzeyi (X±SS) sırasıyla 103.4 ±22.3 ve 39.0±8.-1 ug/L olarak bulundu. St E yönteminin kullanıldığı MD serum (n=20) ve direkt serum (n=20)lu tekniklerde ise bu değer 35.9±9.7 ve 68.8± 16.7 idi. StBG ve StE yöntemleriyle-direkt kullanılan serumlarda deney-için % CV değerleri sırasıyla 27. 1 ve 20.3 olarak bulundu. Bu değer St E yöntemiyle MD:'li. teknikte 8. 3, StEG yönteminin kullanıldığı PP tekniğinde 6.3 olarak saptandı; MD 'li (St E- yöntemi) ve PP'tekniklerinin (StEG yöntemi)% "recovery" değerleri sırasıyla normal serum havuzunda 86,7 ve 104; yüksek serum havuzunda se 94.3 ve 101.7 idi. StEG 'nin kullanıldığı PP tekniğiyle daha iyi:% "recovery" ve % CV değerlerinin elde edildiği ve kullanımının daha güvenilir olduğu sonucuna varıldı.
In this study standard addition (StA) and standard curve (StC) methods are investigated and compared with each other for the determination of serum Al levels. Measurements are made in an atomic absorption spectrophotometer after applying the same sera two different preanalytical preparations as well as using the serum directly. These three preanalytical techniques [direct, matrix modifier (MM) and protein precipitation (PP)] are compared with each other, also. The mean Allevels were measured as (X±SD) 103.4±22.3 and 39.0±8.4 ug/L with the techniques of direct serum (n=20) and PP (n=20) in StC method, respectively. ln the method of StA by the techniques of MM and direct serum, the levels were found to be as 35. 9±9.7 rand 68.8± 16.7 ug/L, respectively. Within-day CV % results were 27. 1 and 20.3 for the direct serum technique in StC and StA methods, respectively. On the other hand, these results were 8.3 f or MM technique in StA, and 6.3 f or PP technique in StC. Recovery (%) results were found to be in normal serımı pool as 86.7 and 104 while in high serum pool as 94.3 and 101.7 respectively for the MM (StA method) and PP (StC method) techniques. Finally, it is concluded that the use of PP technique in the StC method might be more reliable because of the beller recovery and CV % results obtained by that way.
In this study standard addition (StA) and standard curve (StC) methods are investigated and compared with each other for the determination of serum Al levels. Measurements are made in an atomic absorption spectrophotometer after applying the same sera two different preanalytical preparations as well as using the serum directly. These three preanalytical techniques [direct, matrix modifier (MM) and protein precipitation (PP)] are compared with each other, also. The mean Allevels were measured as (X±SD) 103.4±22.3 and 39.0±8.4 ug/L with the techniques of direct serum (n=20) and PP (n=20) in StC method, respectively. ln the method of StA by the techniques of MM and direct serum, the levels were found to be as 35. 9±9.7 rand 68.8± 16.7 ug/L, respectively. Within-day CV % results were 27. 1 and 20.3 for the direct serum technique in StC and StA methods, respectively. On the other hand, these results were 8.3 f or MM technique in StA, and 6.3 f or PP technique in StC. Recovery (%) results were found to be in normal serımı pool as 86.7 and 104 while in high serum pool as 94.3 and 101.7 respectively for the MM (StA method) and PP (StC method) techniques. Finally, it is concluded that the use of PP technique in the StC method might be more reliable because of the beller recovery and CV % results obtained by that way.
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Keywords
Aluminyum, Atomik absorpsiyon spektrofotometri, Aluminium, Atomic absorption spectrophotometry
Citation
Dirican, M. ve Güler, A. H. (1995). ''Serum aluminyum tayininde standart eğri grafiği ve standart ekleme yöntemlerinin kullanımı''. Uludağ Üniversitesi Tıp Fakültesi Dergisi, 22(1-2-3), 67-71.