Determination of total phenolic content of Prunella L. by immobilized enzyme bioreactor

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dc.contributor.buuauthorŞahin, Saliha
dc.contributor.buuauthorDemir, Cevdet
dc.contributor.buuauthorMalyer, Hulusi M.
dc.contributor.departmentUludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Anabilim Dalı.tr_TR
dc.contributor.departmentUludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Anabilim Dalı.tr_TR
dc.contributor.orcid0000-0003-1508-0181tr_TR
dc.contributor.orcid0000-0002-9381-0410tr_TR
dc.contributor.researcheridABA-2005-2020tr_TR
dc.contributor.researcheridAAH-2892-2021tr_TR
dc.contributor.scopusid42960979100tr_TR
dc.contributor.scopusid7003565902tr_TR
dc.contributor.scopusid6602736554tr_TR
dc.date.accessioned2022-01-05T06:42:48Z
dc.date.available2022-01-05T06:42:48Z
dc.date.issued2011-04
dc.description.abstractA new method for determination of total phenolic content of four Prunella L. species was developed by immobilizing horseradish peroxidase (HRP) on styrene-divinylbenzene-polygluteraldehyde (STY-DVB- PGA) polymer. The method is based on the spectrophotometric measurement of the final quinone-imine colored product, absorbing at 510 nm, by horseradish peroxidase oxidation in presence of hydrogen peroxide. The effects of solvents were investigated for the extraction of phenolic compounds in Prunella L. plants. HRP was covalently attached on the STY-DVB-PGA with 90% efficiency at optimum immobilization conditions. The total phenol contents ranged from 4 to 152 mg GAE per g of dried sample for pure solvents and ranged from 19 to 763 mg GAE per g of dried sample for acidic extracts using the immobilized enzymatic method. STY-DVB-PGA beads are a good potential support for the immobilization of HRP enzyme and can be used for determination of the total phenolic content of plants. In the analysis of four Prunella L. species, high correlations were obtained between enzymatic and Folin methods, supporting the validity of the enzymatic method proposed. The enzymatic (free/immobilized) methods are more specific and rapid than the Folin method. Acidic solvents are more effective than pure solvents for extraction of phenolic compounds in Prunella L. species.en_US
dc.identifier.citationŞahin, S. vd. (2011). "Determination of total phenolic content of Prunella L. by immobilized enzyme bioreactor". Analytical Methods, 3(4), 944-950.en_US
dc.identifier.endpage950tr_TR
dc.identifier.issn1759-9660
dc.identifier.issue4tr_TR
dc.identifier.scopus2-s2.0-79959761335tr_TR
dc.identifier.startpage944tr_TR
dc.identifier.urihttps://doi.org/10.1039/c0ay00732c
dc.identifier.urihttps://pubs.rsc.org/en/Content/ArticleLanding/2011/AY/C0AY00732C
dc.identifier.urihttp://hdl.handle.net/11452/23856
dc.identifier.volume3tr_TR
dc.identifier.wos000292164200023tr_TR
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherRoyal Soc Chemistryen_US
dc.relation.bap2009/38tr_TR
dc.relation.journalAnalytical Methodsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectChemistryen_US
dc.subjectFood science & technologyen_US
dc.subjectSpectroscopyen_US
dc.subjectAntioxidant capacityen_US
dc.subjectVulgaris extracten_US
dc.subjectRosmarinic aciden_US
dc.subjectOptimizationen_US
dc.subjectEnzyme immobilizationen_US
dc.subjectHydrogen peroxideen_US
dc.subjectOxidationen_US
dc.subjectSolvent extractionen_US
dc.subjectSolventsen_US
dc.subjectStyreneen_US
dc.subjectAcidic solventen_US
dc.subjectColored productsen_US
dc.subjectEnzymatic methodsen_US
dc.subjectHorse-radish peroxidaseen_US
dc.subjectImmobilization conditionsen_US
dc.subjectImmobilized enzymeen_US
dc.subjectPhenolic compoundsen_US
dc.subjectPure solventsen_US
dc.subjectSpectrophotometric measurementsen_US
dc.subjectTotal phenolic contenten_US
dc.subjectTotal phenolsen_US
dc.subjectPhenolsen_US
dc.subjectFreshen_US
dc.subject.scopusPrunella; Machaeranthera; Derrisen_US
dc.subject.wosChemistry, analyticalen_US
dc.subject.wosFood science & technologyen_US
dc.subject.wosSpectroscopyen_US
dc.titleDetermination of total phenolic content of Prunella L. by immobilized enzyme bioreactoren_US
dc.typeArticle
dc.wos.quartileQ3en_US
dc.wos.quartileQ2 (Food science & technology)en_US

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