Brucella abortus L7/L12 recombinant protein induces strong Th1 response in acute brucellosis patients

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dc.contributor.authorOliveira, Sergio
dc.contributor.buuauthorKazak, Esra
dc.contributor.buuauthorGöral, Güher
dc.contributor.buuauthorAkalın, Halis
dc.contributor.buuauthorYılmaz, Emel
dc.contributor.buuauthorHeper, Yasemin
dc.contributor.buuauthorOral, Haluk Barbaros
dc.contributor.departmentUludağ Üniversitesi/Tıp Fakültesi/Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Anabilim Dalı.tr_TR
dc.contributor.departmentUludağ Üniversitesi/Tıp Fakültesi/ Tıbbi Mikrobiyoloji Anabilim Dalı.tr_TR
dc.contributor.orcid0000-0003-0463-6818tr_TR
dc.contributor.researcheridK-7285-2012tr_TR
dc.contributor.researcheridAAG-8459-2021tr_TR
dc.contributor.researcheridAAH-6506-2021tr_TR
dc.contributor.researcheridAAU-8952-2020tr_TR
dc.contributor.scopusid24921238200tr_TR
dc.contributor.scopusid6603453166tr_TR
dc.contributor.scopusid57207553671tr_TR
dc.contributor.scopusid22037135100tr_TR
dc.contributor.scopusid56191003300tr_TR
dc.contributor.scopusid7004498001tr_TR
dc.date.accessioned2022-04-15T13:05:35Z
dc.date.available2022-04-15T13:05:35Z
dc.date.issued2010-09
dc.description.abstractBackground: Because of high morbidity of the brucellosis in humans and the potential use of the microorganism as an agent of biologic warfare, protection of effective vaccines and specific diagnostic reagents become necessary to eradicate brucellosis. Objective: In this study we aimed to investigate the cytokine responses and changes in peripheral blood lymphocyte subgroups of acute brucellosis patients in response to L7/L12 and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) recombinant proteins derived from Brucella abortus. Methods: levels of IFN-gamma, IL-4 and IL-10 secreted from PBMCs of 25 acute brucellosis patients and 15 healthy controls, stimulated with Phytohemagglutinin (PHA), L7/L12 or GAPDH were measured by ELISA. Furthermore alterations in lymphocyte subgroups in response to these Brucella antigens were determined by flow cytometry. Results: Extracellular IFN-gamma levels were found to be elevated after stimulation with L7/L12 in patients with acute brucellosis, whereas no significant changes were found in IL-4 and IL-10 levels. Similar data was also obtained with GAPDH, but the stimulation of IFN-gamma production was not observed in all patients and was not as strong as that observed for L7/L12. Moreover, when the distribution of lymphocytes subgroups (CD3(+), CD3(+)CD4(+), CD3(+)CD8(+), CD4(+)CD25(+), CD3(+)CD69(+) and CD3(+)CD152(+)) was evaluated, it was found that the stimulation with L7/L12 and GAPDH only led to an increase in the percentage of CD3(+)CD69(+) lymphocytes. Conclusion: These data indicate that Brucella abortus L7/L12 or GAPDH induce a Th1 type immune response in acute brucellosis patients. Additionally, these recombinant proteins, especially L7/L12, may be used in new vaccine preparations and diagnostic tests.en_US
dc.identifier.citationKazak, E. vd. (2010). "Brucella abortus L7/L12 recombinant protein induces strong Th1 response in acute brucellosis patients". Iranian Journal of Immunology, 7(3), 132-141.en_US
dc.identifier.endpage141tr_TR
dc.identifier.issn1735-1383
dc.identifier.issn1735-367X
dc.identifier.issue3tr_TR
dc.identifier.pubmed20876984tr_TR
dc.identifier.scopus2-s2.0-79952666425tr_TR
dc.identifier.startpage132tr_TR
dc.identifier.urihttps://iji.sums.ac.ir/article_17050_0.html
dc.identifier.urihttp://hdl.handle.net/11452/25810
dc.identifier.volume7tr_TR
dc.identifier.wos000208406400001tr_TR
dc.indexed.pubmedPubMeden_US
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherShiraz Institute of Cancer Researchen_US
dc.relation.collaborationYurt dışıtr_TR
dc.relation.journalIranian Journal of Immunologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBrucellosisen_US
dc.subjectCytokineen_US
dc.subjectRecombinant proteinsen_US
dc.subjectT lymphocytesen_US
dc.subjectImmunologyen_US
dc.subject.emtreeGamma interferonen_US
dc.subject.emtreeGlyceraldehyde 3 phosphate dehydrogenaseen_US
dc.subject.emtreeInterleukin 10en_US
dc.subject.emtreeInterleukin 4en_US
dc.subject.emtreeRecombinant proteinen_US
dc.subject.emtreeRecombinant protein L7 L12en_US
dc.subject.emtreeUnclassified drugen_US
dc.subject.emtreeAdulten_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeBrucella abortusen_US
dc.subject.emtreeBrucellosisen_US
dc.subject.emtreeCD3+ T lymphocyteen_US
dc.subject.emtreeCD4+ CD25+ T lymphocyteen_US
dc.subject.emtreeCD4+ T lymphocyteen_US
dc.subject.emtreeCD8+ T lymphocyteen_US
dc.subject.emtreeCellular distributionen_US
dc.subject.emtreeCellular immunityen_US
dc.subject.emtreeClinical articleen_US
dc.subject.emtreeControlled studyen_US
dc.subject.emtreeCytokine releaseen_US
dc.subject.emtreeEnzyme linked immunosorbent assayen_US
dc.subject.emtreeFemaleen_US
dc.subject.emtreeFlow cytometryen_US
dc.subject.emtreeHumanen_US
dc.subject.emtreeHuman cellen_US
dc.subject.emtreeLymphocyteen_US
dc.subject.emtreeMaleen_US
dc.subject.emtreePeripheral lymphocyteen_US
dc.subject.emtreeProtein blood levelen_US
dc.subject.emtreeSerologyen_US
dc.subject.emtreeTh1 cellen_US
dc.subject.meshAdulten_US
dc.subject.meshBrucella abortusen_US
dc.subject.meshBrucellosisen_US
dc.subject.meshCase-control studiesen_US
dc.subject.meshCytokinesen_US
dc.subject.meshFemaleen_US
dc.subject.meshGlyceraldehyde-3-phosphate dehydrogenase (phosphorylating)en_US
dc.subject.meshHumansen_US
dc.subject.meshLeukocytes, mononuclearen_US
dc.subject.meshLymphocyte subsetsen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle ageden_US
dc.subject.meshRecombinant proteinsen_US
dc.subject.meshRibosomal proteinsen_US
dc.subject.meshTh1 cellsen_US
dc.subject.scopusBrucella Abortus; Zoonosis; Bovine Brucellosisen_US
dc.subject.wosImmunologyen_US
dc.titleBrucella abortus L7/L12 recombinant protein induces strong Th1 response in acute brucellosis patientsen_US
dc.typeArticle

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