L-Karnitin ilave edilmiş sulandırıcıların dondurma-çözdürme sonrası teke spermasının spermatolojik parametleri üzerine etkisis
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Date
2022-07-26
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Bursa Uludağ Üniversitesi
Abstract
Bu çalışmada, L-karnitinin (LC) dondurma-eritme sonrası teke sperması üzerindeki etkilerinin değerlendirilmesi amaçlandı. Ergin tekelerden elde edilen sperma örnekleri pooling yapıldı ve beş eşit hacme bölündü. Farklı konsantrasyonlarda LC içeren (2.5mM /5mM/ 7.5mM/ 10 mM) ve içermeyen (kontrol) sulandırıcılar ile sulandırıldı ve donduruldu. Gruplara ait spermatozoa motilitesi, plazma membran fonksiyonel bütünlüğü (HOST), akrozomal bütünlük (PSA-FITC) ve malondialdehit konsantrasyonu belirlendi. Eritme sonrası motilite değerleri; LC5 (L-karnitin 5mMol) ve LC7,5 (L-karnitin 7,5mMol) gruplarında kontrol grubuna göre daha üstün bulundu (P<0.05). Plazma membran bütünlüğü değerlendirildiğinde LC5 grubu kontrol grubu ve diğer LC gruplarına göre membran bütünlüğünü daha iyi koruduğu görüldü (P<0.05). Akrozomal bütünlük oranları LC5 ve LC7,5 gruplarında diğer tüm gruplara göre daha iyi bulundu (P<0.05). Malondialdehit (MDA) konsantrasyonu, membran lipid peroksidasyonunu analiz etmek için kullanıldı ve eritme sonrası kontrol grubuna kıyasla LC5 grubunda daha iyi bir sonuç elde edildi (P<0.05). Çalışmada, sulandırıcıya LC ilave edilmesinin teke spermatolojik parametreleri üzerinde faydalı etkisi olduğu görüldü. Ayrıca, L-karnitinin 5mM dozda kullanımının, diğer dozlara göre daha etkin koruma sağladığı sonucuna varılmıştır.
In this study, it was aimed to evaluate the effects of L-carnitine (LC) on goat spermatozoa after thawing. Sperm samples from sexually mature goat were pooled and divided into five equal volumes. Diluted with diluents added at different concentrations of LC (2.5mM /5 mM/ 7.5mM/10 mM) and without (control) and frozen. Sperm motility, plasma membrane functional integrity (HOST), acrosomal integrity (PSAFITC) and malondialdehyde concentration of the groups were determined. Motility values post thaw; LC5 (L-Carnitin 5mMol) and LC7,5 (L-Carnitin 7,5mMol) groups had a possitive effect on compared to the the control group (P<0.05). The plasma membrane integrity was evaluated, it was observed that the LC5 group preserved the membrane integrity better than the control group and other LC groups (P<0.05). Acrosome integrity rates were better in the LC5 and LC7.5 groups than in the other groups (P<0.05). The malondialdehyde (MDA) concentration was used to analyze the membrane lipid peroxidation and a better result was obtained in the LC5 group compared to the post-thaw control group. (P<0.05). The study showed that the addition of LC to the extender had a beneficial effect on goat spermatological parameters. In addition, it was concluded that the use of carnitine at a dose of 5mMol provides more effective preservation than other doses.
In this study, it was aimed to evaluate the effects of L-carnitine (LC) on goat spermatozoa after thawing. Sperm samples from sexually mature goat were pooled and divided into five equal volumes. Diluted with diluents added at different concentrations of LC (2.5mM /5 mM/ 7.5mM/10 mM) and without (control) and frozen. Sperm motility, plasma membrane functional integrity (HOST), acrosomal integrity (PSAFITC) and malondialdehyde concentration of the groups were determined. Motility values post thaw; LC5 (L-Carnitin 5mMol) and LC7,5 (L-Carnitin 7,5mMol) groups had a possitive effect on compared to the the control group (P<0.05). The plasma membrane integrity was evaluated, it was observed that the LC5 group preserved the membrane integrity better than the control group and other LC groups (P<0.05). Acrosome integrity rates were better in the LC5 and LC7.5 groups than in the other groups (P<0.05). The malondialdehyde (MDA) concentration was used to analyze the membrane lipid peroxidation and a better result was obtained in the LC5 group compared to the post-thaw control group. (P<0.05). The study showed that the addition of LC to the extender had a beneficial effect on goat spermatological parameters. In addition, it was concluded that the use of carnitine at a dose of 5mMol provides more effective preservation than other doses.
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Keywords
Teke, Sperma, Dondurma, L-karnitin, Goat, Semen, Cryopreservation, L-carnitine
Citation
Aktar, A. ve Alçay, S. (2022). "L-Karnitin ilave edilmiş sulandırıcıların dondurma-çözdürme Sonrası teke spermasının spermatolojik parametleri üzerine etkisi". Veteriner Hekimlikte Araştırma Dergisi, 41(1), 37-42.