Addition of niclosamide to palladium(II) saccharinate complex of terpyridine results in enhanced cytotoxic activity inducing apoptosis on cancer stem cells of breast cancer
dc.contributor.buuauthor | Karakaş, Didem | |
dc.contributor.buuauthor | Cevatemre, Buse | |
dc.contributor.buuauthor | Aztopal, Nazlıhan | |
dc.contributor.buuauthor | Arı, Ferda | |
dc.contributor.buuauthor | Yılmaz, Veysel Turan | |
dc.contributor.buuauthor | Ulukaya, Engin | |
dc.contributor.department | Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü. | tr_TR |
dc.contributor.department | Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü. | tr_TR |
dc.contributor.department | Uludağ Üniversitesi/Tıp Fakültesi/Klinik Biyokimya Anabilim Dalı. | tr_TR |
dc.contributor.orcid | 0000-0003-3118-8061 | tr_TR |
dc.contributor.orcid | 0000-0002-2849-3332 | tr_TR |
dc.contributor.orcid | 0000-0002-3781-6834 | tr_TR |
dc.contributor.orcid | 0000-0002-6729-7908 | tr_TR |
dc.contributor.researcherid | L-6687-2018 | tr_TR |
dc.contributor.researcherid | K-5792-2018 | tr_TR |
dc.contributor.researcherid | AHD-2050-2022 | tr_TR |
dc.contributor.researcherid | L-7238-2018 | tr_TR |
dc.contributor.researcherid | L-6682-2018 | tr_TR |
dc.contributor.researcherid | AAG-7012-2021 | tr_TR |
dc.contributor.researcherid | AAV-4886-2020 | tr_TR |
dc.contributor.scopusid | 56422040600 | tr_TR |
dc.contributor.scopusid | 55693788600 | tr_TR |
dc.contributor.scopusid | 55853882900 | tr_TR |
dc.contributor.scopusid | 24376085300 | tr_TR |
dc.contributor.scopusid | 56441123900 | tr_TR |
dc.contributor.scopusid | 6602927353 | tr_TR |
dc.date.accessioned | 2022-06-13T12:21:44Z | |
dc.date.available | 2022-06-13T12:21:44Z | |
dc.date.issued | 2015-09-01 | |
dc.description.abstract | Wnt signaling is one of the core signaling pathways of cancer stem cells (CSCs). It is re-activated in CSCs and plays essential role in the survival, self-renewal and proliferation of these cells. Therefore, we aimed to evaluate the cytotoxic effects of palladium(II) complex which is formulated as [PdCl(terpy)](sac)2H(2)O and its combination with niclosamide which is an inhibitor of Wnt signaling pathway associated with breast cancer stem cells. Characteristic cell surface markers (CD44(+)/CD24(-)) were determined by flow cytometry in CSCs. ATP viability assay was used to determine the cytotoxic activity. The mode of cell death was evaluated morphologically using fluorescence microscopy and biochemically using M30 ELISA assay as well as performing qPCR. Our study demonstrated that the combination of niclosamide (1.5 mu M) and Pd(II) complex (12.5, 25 and 50 mu M) at 48 h has enhanced cytotoxic activity resulted from the induction of apoptosis (indicated by the presence of pyknotic nuclei, increments in M30 and over expression of proapoptotic genes of TNFRSF10A and FAS). Importantly, the addition of niclosamide resulted in the suppression of autophagy (proved by the decrease in ATG5 gene levels) that might have contributed to the enhanced cytotoxicity. In conclusion, the application of this combination may be regarded as a novel and effective approach for the treatment of breast cancer due to its promising cytotoxic effect on cancer stem cells that cause recurrence of the disease. | en_US |
dc.identifier.citation | Karakaş, D. vd. (2015). "Addition of niclosamide to palladium(II) saccharinate complex of terpyridine results in enhanced cytotoxic activity inducing apoptosis on cancer stem cells of breast cancer". Bioorganic and Medicinal Chemistry, 23(17), 5580-5586. | en_US |
dc.identifier.endpage | 5586 | tr_TR |
dc.identifier.issn | 0968-0896 | |
dc.identifier.issue | 17 | tr_TR |
dc.identifier.pubmed | 26234907 | tr_TR |
dc.identifier.scopus | 2-s2.0-84946491675 | tr_TR |
dc.identifier.startpage | 5580 | tr_TR |
dc.identifier.uri | https://doi.org/10.1016/j.bmc.2015.07.026 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0968089615006100 | |
dc.identifier.uri | http://hdl.handle.net/11452/27109 | |
dc.identifier.volume | 23 | tr_TR |
dc.identifier.wos | 000360349900035 | tr_TR |
dc.indexed.pubmed | PubMed | en_US |
dc.indexed.scopus | Scopus | en_US |
dc.indexed.wos | SCIE | en_US |
dc.language.iso | en | en_US |
dc.publisher | Pergamon-Elsevier | en_US |
dc.relation.journal | Bioorganic and Medicinal Chemistry | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | tr_TR |
dc.relation.tubitak | 212T147 | tr_TR |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Breast cancer stem cell | en_US |
dc.subject | Cytotoxicity | en_US |
dc.subject | Niclosamide | en_US |
dc.subject | Palladium(II) complex | en_US |
dc.subject | Targeted therapy | en_US |
dc.subject | In-vitro | en_US |
dc.subject | Platinum(II) complexes | en_US |
dc.subject | Antitumor-activity | en_US |
dc.subject | Inhibition | en_US |
dc.subject | Carcinoma | en_US |
dc.subject | Tumors | en_US |
dc.subject | Agent | en_US |
dc.subject | Vivo | en_US |
dc.subject | Biochemistry & molecular biology | en_US |
dc.subject | Pharmacology & pharmacy | en_US |
dc.subject | Chemistry | en_US |
dc.subject.emtree | Autophagy protein 5 | en_US |
dc.subject.emtree | CD24 antigen | en_US |
dc.subject.emtree | Fas antigen | en_US |
dc.subject.emtree | Hermes antigen | en_US |
dc.subject.emtree | Niclosamide | en_US |
dc.subject.emtree | Palladium complex | en_US |
dc.subject.emtree | Palladium saccharinate complex | en_US |
dc.subject.emtree | Pyridine | en_US |
dc.subject.emtree | Terpyridine | en_US |
dc.subject.emtree | Unclassified drug | en_US |
dc.subject.emtree | Niclosamide | en_US |
dc.subject.emtree | Palladium | en_US |
dc.subject.emtree | Antigen expression | en_US |
dc.subject.emtree | Antineoplastic activity | en_US |
dc.subject.emtree | Apoptosis | en_US |
dc.subject.emtree | Article | en_US |
dc.subject.emtree | Assay | en_US |
dc.subject.emtree | ATG5 gene | en_US |
dc.subject.emtree | ATP viability assay | en_US |
dc.subject.emtree | Autophagy | en_US |
dc.subject.emtree | Breast cancer | en_US |
dc.subject.emtree | Cancer inhibition | en_US |
dc.subject.emtree | Cancer stem cell | en_US |
dc.subject.emtree | Cell death | en_US |
dc.subject.emtree | Controlled study | en_US |
dc.subject.emtree | Drug cytotoxicity | en_US |
dc.subject.emtree | Drug efficacy | en_US |
dc.subject.emtree | Drug potentiation | en_US |
dc.subject.emtree | Enzyme linked immunosorbent assay | en_US |
dc.subject.emtree | FAS gene | en_US |
dc.subject.emtree | Flow cytometry | en_US |
dc.subject.emtree | Fluorescence microscopy | en_US |
dc.subject.emtree | Gene | en_US |
dc.subject.emtree | Gene overexpression | en_US |
dc.subject.emtree | MLKL gene | en_US |
dc.subject.emtree | Necroptosis | en_US |
dc.subject.emtree | Real time polymerase chain reaction | en_US |
dc.subject.emtree | TNFRSF10A gene | en_US |
dc.subject.emtree | Upregulation | en_US |
dc.subject.emtree | Wnt signaling pathway | en_US |
dc.subject.emtree | Apoptosis | en_US |
dc.subject.emtree | Breast neoplasms | en_US |
dc.subject.emtree | Drug effects | en_US |
dc.subject.emtree | Female | en_US |
dc.subject.emtree | Human | en_US |
dc.subject.emtree | Pathology | en_US |
dc.subject.mesh | Apoptosis | en_US |
dc.subject.mesh | Breast neoplasms | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Neoplastic stem cells | en_US |
dc.subject.mesh | Niclosamide | en_US |
dc.subject.mesh | Palladium | en_US |
dc.subject.mesh | Wnt signaling pathway | en_US |
dc.subject.scopus | Complex; Palladium; 2-Phenylpyridine | en_US |
dc.subject.wos | Biochemistry & molecular biology | en_US |
dc.subject.wos | Chemistry, medicinal | en_US |
dc.subject.wos | Chemistry, organic | en_US |
dc.title | Addition of niclosamide to palladium(II) saccharinate complex of terpyridine results in enhanced cytotoxic activity inducing apoptosis on cancer stem cells of breast cancer | en_US |
dc.type | Article | |
dc.wos.quartile | Q2 | en_US |
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