Addition of niclosamide to palladium(II) saccharinate complex of terpyridine results in enhanced cytotoxic activity inducing apoptosis on cancer stem cells of breast cancer

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dc.contributor.buuauthorKarakaş, Didem
dc.contributor.buuauthorCevatemre, Buse
dc.contributor.buuauthorAztopal, Nazlıhan
dc.contributor.buuauthorArı, Ferda
dc.contributor.buuauthorYılmaz, Veysel Turan
dc.contributor.buuauthorUlukaya, Engin
dc.contributor.departmentUludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.tr_TR
dc.contributor.departmentUludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü.tr_TR
dc.contributor.departmentUludağ Üniversitesi/Tıp Fakültesi/Klinik Biyokimya Anabilim Dalı.tr_TR
dc.contributor.orcid0000-0003-3118-8061tr_TR
dc.contributor.orcid0000-0002-2849-3332tr_TR
dc.contributor.orcid0000-0002-3781-6834tr_TR
dc.contributor.orcid0000-0002-6729-7908tr_TR
dc.contributor.researcheridL-6687-2018tr_TR
dc.contributor.researcheridK-5792-2018tr_TR
dc.contributor.researcheridAHD-2050-2022tr_TR
dc.contributor.researcheridL-7238-2018tr_TR
dc.contributor.researcheridL-6682-2018tr_TR
dc.contributor.researcheridAAG-7012-2021tr_TR
dc.contributor.researcheridAAV-4886-2020tr_TR
dc.contributor.scopusid56422040600tr_TR
dc.contributor.scopusid55693788600tr_TR
dc.contributor.scopusid55853882900tr_TR
dc.contributor.scopusid24376085300tr_TR
dc.contributor.scopusid56441123900tr_TR
dc.contributor.scopusid6602927353tr_TR
dc.date.accessioned2022-06-13T12:21:44Z
dc.date.available2022-06-13T12:21:44Z
dc.date.issued2015-09-01
dc.description.abstractWnt signaling is one of the core signaling pathways of cancer stem cells (CSCs). It is re-activated in CSCs and plays essential role in the survival, self-renewal and proliferation of these cells. Therefore, we aimed to evaluate the cytotoxic effects of palladium(II) complex which is formulated as [PdCl(terpy)](sac)2H(2)O and its combination with niclosamide which is an inhibitor of Wnt signaling pathway associated with breast cancer stem cells. Characteristic cell surface markers (CD44(+)/CD24(-)) were determined by flow cytometry in CSCs. ATP viability assay was used to determine the cytotoxic activity. The mode of cell death was evaluated morphologically using fluorescence microscopy and biochemically using M30 ELISA assay as well as performing qPCR. Our study demonstrated that the combination of niclosamide (1.5 mu M) and Pd(II) complex (12.5, 25 and 50 mu M) at 48 h has enhanced cytotoxic activity resulted from the induction of apoptosis (indicated by the presence of pyknotic nuclei, increments in M30 and over expression of proapoptotic genes of TNFRSF10A and FAS). Importantly, the addition of niclosamide resulted in the suppression of autophagy (proved by the decrease in ATG5 gene levels) that might have contributed to the enhanced cytotoxicity. In conclusion, the application of this combination may be regarded as a novel and effective approach for the treatment of breast cancer due to its promising cytotoxic effect on cancer stem cells that cause recurrence of the disease.en_US
dc.identifier.citationKarakaş, D. vd. (2015). "Addition of niclosamide to palladium(II) saccharinate complex of terpyridine results in enhanced cytotoxic activity inducing apoptosis on cancer stem cells of breast cancer". Bioorganic and Medicinal Chemistry, 23(17), 5580-5586.en_US
dc.identifier.endpage5586tr_TR
dc.identifier.issn0968-0896
dc.identifier.issue17tr_TR
dc.identifier.pubmed26234907tr_TR
dc.identifier.scopus2-s2.0-84946491675tr_TR
dc.identifier.startpage5580tr_TR
dc.identifier.urihttps://doi.org/10.1016/j.bmc.2015.07.026
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0968089615006100
dc.identifier.urihttp://hdl.handle.net/11452/27109
dc.identifier.volume23tr_TR
dc.identifier.wos000360349900035tr_TR
dc.indexed.pubmedPubMeden_US
dc.indexed.scopusScopusen_US
dc.indexed.wosSCIEen_US
dc.language.isoenen_US
dc.publisherPergamon-Elsevieren_US
dc.relation.journalBioorganic and Medicinal Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergitr_TR
dc.relation.tubitak212T147tr_TR
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBreast cancer stem cellen_US
dc.subjectCytotoxicityen_US
dc.subjectNiclosamideen_US
dc.subjectPalladium(II) complexen_US
dc.subjectTargeted therapyen_US
dc.subjectIn-vitroen_US
dc.subjectPlatinum(II) complexesen_US
dc.subjectAntitumor-activityen_US
dc.subjectInhibitionen_US
dc.subjectCarcinomaen_US
dc.subjectTumorsen_US
dc.subjectAgenten_US
dc.subjectVivoen_US
dc.subjectBiochemistry & molecular biologyen_US
dc.subjectPharmacology & pharmacyen_US
dc.subjectChemistryen_US
dc.subject.emtreeAutophagy protein 5en_US
dc.subject.emtreeCD24 antigenen_US
dc.subject.emtreeFas antigenen_US
dc.subject.emtreeHermes antigenen_US
dc.subject.emtreeNiclosamideen_US
dc.subject.emtreePalladium complexen_US
dc.subject.emtreePalladium saccharinate complexen_US
dc.subject.emtreePyridineen_US
dc.subject.emtreeTerpyridineen_US
dc.subject.emtreeUnclassified drugen_US
dc.subject.emtreeNiclosamideen_US
dc.subject.emtreePalladiumen_US
dc.subject.emtreeAntigen expressionen_US
dc.subject.emtreeAntineoplastic activityen_US
dc.subject.emtreeApoptosisen_US
dc.subject.emtreeArticleen_US
dc.subject.emtreeAssayen_US
dc.subject.emtreeATG5 geneen_US
dc.subject.emtreeATP viability assayen_US
dc.subject.emtreeAutophagyen_US
dc.subject.emtreeBreast canceren_US
dc.subject.emtreeCancer inhibitionen_US
dc.subject.emtreeCancer stem cellen_US
dc.subject.emtreeCell deathen_US
dc.subject.emtreeControlled studyen_US
dc.subject.emtreeDrug cytotoxicityen_US
dc.subject.emtreeDrug efficacyen_US
dc.subject.emtreeDrug potentiationen_US
dc.subject.emtreeEnzyme linked immunosorbent assayen_US
dc.subject.emtreeFAS geneen_US
dc.subject.emtreeFlow cytometryen_US
dc.subject.emtreeFluorescence microscopyen_US
dc.subject.emtreeGeneen_US
dc.subject.emtreeGene overexpressionen_US
dc.subject.emtreeMLKL geneen_US
dc.subject.emtreeNecroptosisen_US
dc.subject.emtreeReal time polymerase chain reactionen_US
dc.subject.emtreeTNFRSF10A geneen_US
dc.subject.emtreeUpregulationen_US
dc.subject.emtreeWnt signaling pathwayen_US
dc.subject.emtreeApoptosisen_US
dc.subject.emtreeBreast neoplasmsen_US
dc.subject.emtreeDrug effectsen_US
dc.subject.emtreeFemaleen_US
dc.subject.emtreeHumanen_US
dc.subject.emtreePathologyen_US
dc.subject.meshApoptosisen_US
dc.subject.meshBreast neoplasmsen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshNeoplastic stem cellsen_US
dc.subject.meshNiclosamideen_US
dc.subject.meshPalladiumen_US
dc.subject.meshWnt signaling pathwayen_US
dc.subject.scopusComplex; Palladium; 2-Phenylpyridineen_US
dc.subject.wosBiochemistry & molecular biologyen_US
dc.subject.wosChemistry, medicinalen_US
dc.subject.wosChemistry, organicen_US
dc.titleAddition of niclosamide to palladium(II) saccharinate complex of terpyridine results in enhanced cytotoxic activity inducing apoptosis on cancer stem cells of breast canceren_US
dc.typeArticle
dc.wos.quartileQ2en_US

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