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|Title:||Determination of total phenolic content of raspberry and blackberry cultivars by immobilized horseradish peroxidase bioreactor|
|Authors:||Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Anabilim Dalı.|
Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.
Food science & technology
Raspberry (Rubus idaeus)
Blackberry (Rubus fruticosus)
Total phenol content
|Publisher:||Academic Press Inc Elsevier Science|
|Citation:||Işık, E. vd. (2011). "Determination of total phenolic content of raspberry and blackberry cultivars by immobilized horseradish peroxidase bioreactor". Journal of Food Composition and Analysis, 24(7), 944-949.|
|Abstract:||Raspberry and blackberry cultivars were assayed for total phenol content by enzymatic method using immobilized horseradish peroxidase (HRP). The HRP was immobilized on the styrene-divinylbenzene-polygluteraldehyde (STY-DVB-PGA) beads. Total phenol content determined by immobilized and free enzymatic methods was compared with those obtained by applying the Folin-Ciocalteu method. The total phenol content by immobilized HRP method ranged from 254 to 973 mg gallic acid equivalent (GAE) per 100 g fw as well as 105 to 435 mg ellagic acid equivalent (EAE) per 100 g fw in raspberry and blackberry cultivars. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical values were correlated with total phenol content in raspberry using immobilized enzymatic method as GAE (R-2 = 0.7450, P = 0.05) and EAE (R-2 = 0.7449, P = 0.05). All antioxidant activity values were highly correlated with total phenol content in blackberry using free and immobilized enzymatic methods as GAE (R-2 = 0.9777 and 0.9223, P = 0.05 respectively) and EAE (R-2 = 0.9979 and 0.9223, P = 0.05 respectively). A significant additivity was observed using individual phenolic compounds by immobilized enzymatic method as 95% (GAE) and 90% (EAE). These results show that the immobilized enzymatic assay can be applied to determine the total phenol content of berry fruits, which is more specific and not affected by interfering compounds.|
|Appears in Collections:||Scopus|
Web of Science
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