Publication:
The effect of vitamin A on CCl4-induced hepatic injuries in rats: a histochemical, immunohistochemical and ultrastructural study

dc.contributor.buuauthorNoyan, Semiha
dc.contributor.buuauthorÇavuşoğlu, İlkin
dc.contributor.buuauthorMinbay, F. Zehra
dc.contributor.departmentTıp Fakültesi
dc.contributor.departmentHistoloji ve Embriyoloji Ana Bilim Dalı
dc.contributor.orcid0000-0001-5757-8450
dc.contributor.researcheridABC-1475-2020
dc.contributor.scopusid56636269700
dc.contributor.scopusid56009155800
dc.contributor.scopusid12040872100
dc.date.accessioned2021-10-07T06:07:56Z
dc.date.available2021-10-07T06:07:56Z
dc.date.issued2006
dc.description.abstractIn this study, we aimed to investigate the effect of vitamin A on the transformation of the Ito cells to fibrogenic form and suppression of the development of fibrosis. Carbon tetrachloride intoxication was performed on rats for 2, 8, 12 or 20 weeks and 5 x 10(4) IU vitamin A (as retinol palmitate) was injected subcutaneously once every 4 weeks. Ito cells were detected by gold chloride impregnation, as well as desmin and alpha-smooth muscle actin (alpha-SMA) immunohistochemistry. Additionally, all groups were examined ultrastructurally. The number of Ito cells that were labelled positively with gold impregnation decreased in the fibrotic groups; however, alpha-SMA and desmin immunopositive Ito cells increased. The samples from animals that were treated with vitamin A showed an increase in labelling with gold impregnation but a decrease in alpha-SMA immunopositivity. The data showed that vitamin A can prevent hepatic injury, by suppressing the transformation of Ito cells to fibrogenic form. We conclude that vitamin A has potential for the treatment of hepatic fibrotic diseases. alpha-SMA immunohistochemistry was found to be more informative than desmin immunohistochemistry for monitoring Liver fibrosis.
dc.identifier.citationNoyan, S. vd. (2006). ''The effect of vitamin A on CCl4-induced hepatic injuries in rats: a histochemical, immunohistochemical and ultrastructural study''. Acta Histochemica, 107(6), 421-434.
dc.identifier.endpage431
dc.identifier.issn0065-1281
dc.identifier.issue6
dc.identifier.pubmed16256175
dc.identifier.scopus2-s2.0-31544436592
dc.identifier.startpage421
dc.identifier.urihttps://doi.org/10.1016/j.acthis.2005.09.001
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0065128105000838
dc.identifier.urihttp://hdl.handle.net/11452/22258
dc.identifier.volume107
dc.identifier.wos000235733600002
dc.indexed.wosSCIE
dc.language.isoen
dc.publisherElsevier
dc.relation.journalActa Histochemica
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCell biology
dc.subjectIto cells
dc.subjectVitamin A
dc.subjectDesmin
dc.subjectAlpha-smooth muscle actin
dc.subjectAlpha-SMA
dc.subjectFibrosis
dc.subjectCollagen types
dc.subjectTransitional cell
dc.subjectExtracellular-matrix
dc.subjectPerisinusoidal cells
dc.subjectCarbon-tetrachloride
dc.subjectSmooth-muscle-actin
dc.subjectAlcoholic liver-injury
dc.subjectFat-storing cells
dc.subjectAnimalia
dc.subjectLiving systems studies
dc.subjectImpregnation
dc.subjectGold
dc.subjectDiseases
dc.subjectCarbon inorganic compounds
dc.subjectCell culture
dc.subjecta-SMA
dc.subjecta-smooth muscle actin
dc.subjectVitamins
dc.subject.emtreeGold derivative
dc.subject.emtreeSmooth muscle actin
dc.subject.emtreeRetinol
dc.subject.emtreeDesmin
dc.subject.emtreeAntioxidant
dc.subject.emtreeActin
dc.subject.emtreeRetinol
dc.subject.emtreeGold chloride
dc.subject.emtreeDesmin
dc.subject.emtreeCarbon tetrachloride
dc.subject.emtreeAlpha smooth muscle actin
dc.subject.emtreeWistar rat
dc.subject.emtreePathology
dc.subject.emtreeLiver cirrhosis
dc.subject.emtreeLiver cell
dc.subject.emtreeElectron microscopy
dc.subject.emtreeDrug effect
dc.subject.emtreeCytochemistry
dc.subject.emtreeChemistry
dc.subject.emtreeChemically induced disorder
dc.subject.emtreeCell proliferation
dc.subject.emtreeAnimal
dc.subject.emtreeUltrastructure
dc.subject.emtreeRat
dc.subject.emtreeNonhuman
dc.subject.emtreeMicroscopy
dc.subject.emtreeMale
dc.subject.emtreeLiver injury
dc.subject.emtreeLiver fibrosis
dc.subject.emtreeLto cell
dc.subject.emtreeIntoxication
dc.subject.emtreeImmunohistochemistry
dc.subject.emtreeHstopathology
dc.subject.emtreeHistochemistry
dc.subject.emtreeFibrosis
dc.subject.emtreeControlled study
dc.subject.emtreeArticle
dc.subject.emtreeAnimal tissue
dc.subject.emtreeAnimal model
dc.subject.emtreeAnimal experiment
dc.subject.meshVitamin A
dc.subject.meshRats, wistar
dc.subject.meshRats
dc.subject.meshMicroscopy, electron
dc.subject.meshMale
dc.subject.meshLiver cirrhosis
dc.subject.meshImmunohistochemistry
dc.subject.meshHistocytochemistry
dc.subject.meshHepatocytes
dc.subject.meshGold compounds
dc.subject.meshDesmin
dc.subject.meshCell proliferation
dc.subject.meshCarbon tetrachloride
dc.subject.meshAntioxidants
dc.subject.meshAnimals
dc.subject.meshActins
dc.subject.scopusHepatic Stellate Cells; Liver Fibrosis; Thioacetamide
dc.subject.wosCell biology
dc.titleThe effect of vitamin A on CCl4-induced hepatic injuries in rats: a histochemical, immunohistochemical and ultrastructural study
dc.typeArticle
dc.wos.quartileQ4
dspace.entity.typePublication
local.contributor.departmentTıp Fakültesi/Histoloji ve Embriyoloji Ana Bilim Dalı
local.indexed.atPubMed
local.indexed.atWOS

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